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The map label for this gene is bglH [H]

Identifier: 50365431

GI number: 50365431

Start: 719951

End: 721402

Strand: Reverse

Name: bglH [H]

Synonym: Mfl615

Alternate gene names: 50365431

Gene position: 721402-719951 (Counterclockwise)

Preceding gene: 50365432

Following gene: 50365429

Centisome position: 90.95

GC content: 29.34

Gene sequence:

>1452_bases
ATGAAATTTATTAAAGACGATTTTTTATGAGGTGGAGCAACAGCAGCATCTCAGGTAGAAGGAGCATATAATGAAGGCGG
AAAAACTTTAACTCTACTTGAAATGAAACCTTTCTTGCCAATCAAAAATAGAAAAGCTATACATTTTGGAGGTCAAACAA
AAGAAGAAATGCTTGACTCTATTGAAAACAAACAAAATTTGCATTACCCAAAAAGGTTTGGAATTGACTTTTATCATAGA
TATAAAGAAGATATTGCTTTGTTTAAAGAAGCAGGAATGAACATTTATAGAATGTCAATTGCTTGATCAAGAATATTTCC
TAATGGTGATGATGAAAAACCAAATGAAGCGGGTATAGATTTTTATAGAAATGTTTTTGAAGAATGCAAAAAGGCAGGAA
TTAAAGTAATGGTAACTATGCACCACTTTGATACACCTTATGAAATTACAAAAAAATACGGTGGCTGAACAAACAGAAAA
GTAATAGATTTATTTGTAAAGTATGCTAAAACGTTATTTGAAGAATTTGATGATGTTGTAGATTTTTGATTACCATTTAA
CGAAATTAATGTTGCAATTTGAAGTACAGAAGTTGGTTTGGGAGTTTTTGAATCAGATTTTAAAAGTAAAAATGAAAGAG
ATCAAGCGGCTTATCAGGGACTTCACCATCAATTTATTGCACAAGCAAAAGTTATTGAATTAGGTAAAAAGATGTGTAAG
CTAAAAACTAAATTTGGGTGTATGGTTGCAAACATAACAACTTACGCACTAGACTGTAATCCTATAAATGAAATTGAAAA
CTTGAAATCACAACAAATATCTCGTTGATTTTTCTATGATGTAGTTGCTAAAGGTGAGTATCCTACTTACATTAAAAGAT
ATTTTACTGAAAATAATATAAATATTATAATGGAAAATGATGATGAAAAAGTTTTAAAAAATAATACAGTTGATTTCATT
TCATTTAGTTATTACATGTCTGCTACTGTTGCCGTTCAAGGAAAAGACAAGGCATCAGGCAATGTTTCAACTGGAGGAAA
AAACCCATTTCTAGAAGCAACAGAATGAGGATGACAAATTGACCCTATTGGTTTAAGAGTCTCTTTAAATCAAATGTGAG
ATAGATATCAAAAACCATTATTTATTTCAGAAAATGGTATAGGTGTATTAGAAAATCTGGACTCTAATAATACTGTTAAA
GACGATTATAGAATCGATTATTTAGGAAAACATTTTGAACAAATCAATGAGGCAATCAAGGATGGTATTGATGTATTTGG
TTATACAATGTGAACGCCAATTGATGTAGTGTCTGCTTCAACTAATGAAATGTCAAAACGTTATGGAATGATTTATGTTG
ATTATGATGATTATCATAATGGAACAGGAAATAGGTTTAAAAAGAAATCTTTTAACTGATTTCAAAATTTTATGAAAACA
AAAGAATTTTAA

Upstream 100 bases:

>100_bases
GTTAACAACTTTCTATTTATAAAAGTTAAATTTATTTAATACGTAAAATATGCTAAATTTTAAATATTTGAGAAAATCAA
AATATAAAGGAAGTGAAAAT

Downstream 100 bases:

>100_bases
TAGAAAAAACATCAGCTTATTGGACTGATGTTTTTTCTATTTTAAAAGTTTATTAAGTGTGTCACCAAGACCGCCATCTT
CATTTGATAAATCAGTTATA

Product: beta-glucosidase

Products: NA

Alternate protein names: 6-phospho-beta-glucosidase [H]

Number of amino acids: Translated: 483; Mature: 483

Protein sequence:

>483_residues
MKFIKDDFLWGGATAASQVEGAYNEGGKTLTLLEMKPFLPIKNRKAIHFGGQTKEEMLDSIENKQNLHYPKRFGIDFYHR
YKEDIALFKEAGMNIYRMSIAWSRIFPNGDDEKPNEAGIDFYRNVFEECKKAGIKVMVTMHHFDTPYEITKKYGGWTNRK
VIDLFVKYAKTLFEEFDDVVDFWLPFNEINVAIWSTEVGLGVFESDFKSKNERDQAAYQGLHHQFIAQAKVIELGKKMCK
LKTKFGCMVANITTYALDCNPINEIENLKSQQISRWFFYDVVAKGEYPTYIKRYFTENNINIIMENDDEKVLKNNTVDFI
SFSYYMSATVAVQGKDKASGNVSTGGKNPFLEATEWGWQIDPIGLRVSLNQMWDRYQKPLFISENGIGVLENLDSNNTVK
DDYRIDYLGKHFEQINEAIKDGIDVFGYTMWTPIDVVSASTNEMSKRYGMIYVDYDDYHNGTGNRFKKKSFNWFQNFMKT
KEF

Sequences:

>Translated_483_residues
MKFIKDDFL*GGATAASQVEGAYNEGGKTLTLLEMKPFLPIKNRKAIHFGGQTKEEMLDSIENKQNLHYPKRFGIDFYHR
YKEDIALFKEAGMNIYRMSIA*SRIFPNGDDEKPNEAGIDFYRNVFEECKKAGIKVMVTMHHFDTPYEITKKYGG*TNRK
VIDLFVKYAKTLFEEFDDVVDF*LPFNEINVAI*STEVGLGVFESDFKSKNERDQAAYQGLHHQFIAQAKVIELGKKMCK
LKTKFGCMVANITTYALDCNPINEIENLKSQQISR*FFYDVVAKGEYPTYIKRYFTENNINIIMENDDEKVLKNNTVDFI
SFSYYMSATVAVQGKDKASGNVSTGGKNPFLEATE*G*QIDPIGLRVSLNQM*DRYQKPLFISENGIGVLENLDSNNTVK
DDYRIDYLGKHFEQINEAIKDGIDVFGYTM*TPIDVVSASTNEMSKRYGMIYVDYDDYHNGTGNRFKKKSFN*FQNFMKT
KEF
>Mature_483_residues
MKFIKDDFL*GGATAASQVEGAYNEGGKTLTLLEMKPFLPIKNRKAIHFGGQTKEEMLDSIENKQNLHYPKRFGIDFYHR
YKEDIALFKEAGMNIYRMSIA*SRIFPNGDDEKPNEAGIDFYRNVFEECKKAGIKVMVTMHHFDTPYEITKKYGG*TNRK
VIDLFVKYAKTLFEEFDDVVDF*LPFNEINVAI*STEVGLGVFESDFKSKNERDQAAYQGLHHQFIAQAKVIELGKKMCK
LKTKFGCMVANITTYALDCNPINEIENLKSQQISR*FFYDVVAKGEYPTYIKRYFTENNINIIMENDDEKVLKNNTVDFI
SFSYYMSATVAVQGKDKASGNVSTGGKNPFLEATE*G*QIDPIGLRVSLNQM*DRYQKPLFISENGIGVLENLDSNNTVK
DDYRIDYLGKHFEQINEAIKDGIDVFGYTM*TPIDVVSASTNEMSKRYGMIYVDYDDYHNGTGNRFKKKSFN*FQNFMKT
KEF

Specific function: Catalyzes the hydrolysis of aryl-phospho-beta-D- glucosides such as 4-methylumbelliferyl-phospho-beta-D- glucopyranoside (MUG-P), phosphoarbutin and phosphosalicin. Plays a major role in the utilization of arbutin or salicin as the sole carbon source. Bgl

COG id: COG2723

COG function: function code G; Beta-glucosidase/6-phospho-beta-glucosidase/beta-galactosidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 1 family [H]

Homologues:

Organism=Homo sapiens, GI32481206, Length=479, Percent_Identity=25.0521920668058, Blast_Score=124, Evalue=3e-28,
Organism=Homo sapiens, GI110681710, Length=488, Percent_Identity=23.7704918032787, Blast_Score=108, Evalue=7e-24,
Organism=Homo sapiens, GI13273313, Length=464, Percent_Identity=23.2758620689655, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI24497614, Length=461, Percent_Identity=25.5965292841649, Blast_Score=92, Evalue=1e-18,
Organism=Escherichia coli, GI1789070, Length=474, Percent_Identity=43.8818565400844, Blast_Score=375, Evalue=1e-105,
Organism=Escherichia coli, GI2367270, Length=474, Percent_Identity=44.0928270042194, Blast_Score=372, Evalue=1e-104,
Organism=Escherichia coli, GI2367174, Length=481, Percent_Identity=38.2536382536383, Blast_Score=315, Evalue=3e-87,
Organism=Caenorhabditis elegans, GI17552856, Length=496, Percent_Identity=27.8225806451613, Blast_Score=131, Evalue=9e-31,
Organism=Caenorhabditis elegans, GI17539390, Length=477, Percent_Identity=27.0440251572327, Blast_Score=121, Evalue=6e-28,
Organism=Drosophila melanogaster, GI21356577, Length=483, Percent_Identity=23.6024844720497, Blast_Score=95, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001360
- InterPro:   IPR018120
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00232 Glyco_hydro_1 [H]

EC number: =3.2.1.86 [H]

Molecular weight: Translated: 53907; Mature: 53907

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: PS00572 GLYCOSYL_HYDROL_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFIKDDFLGGATAASQVEGAYNEGGKTLTLLEMKPFLPIKNRKAIHFGGQTKEEMLDSI
CCCCHHHCCCCCHHHHHHCCHHCCCCCEEEEEEECCCCCCCCCCEEEECCCCHHHHHHHH
ENKQNLHYPKRFGIDFYHRYKEDIALFKEAGMNIYRMSIASRIFPNGDDEKPNEAGIDFY
HCCCCCCCCHHHCCHHHHHHHHHHHHHHHCCCCEEEEHHHHHCCCCCCCCCCCCCCHHHH
RNVFEECKKAGIKVMVTMHHFDTPYEITKKYGGTNRKVIDLFVKYAKTLFEEFDDVVDFL
HHHHHHHHHCCCEEEEEEECCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
PFNEINVAISTEVGLGVFESDFKSKNERDQAAYQGLHHQFIAQAKVIELGKKMCKLKTKF
CCCCEEEEEEECCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GCMVANITTYALDCNPINEIENLKSQQISRFFYDVVAKGEYPTYIKRYFTENNINIIMEN
CEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEEC
DDEKVLKNNTVDFISFSYYMSATVAVQGKDKASGNVSTGGKNPFLEATEGQIDPIGLRVS
CCHHHHHCCCCCEEEEEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEE
LNQMDRYQKPLFISENGIGVLENLDSNNTVKDDYRIDYLGKHFEQINEAIKDGIDVFGYT
HHHHHHHCCCEEEECCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHCCEEE
MTPIDVVSASTNEMSKRYGMIYVDYDDYHNGTGNRFKKKSFNFQNFMKTKEF
ECCHHHCCCCHHHHHHHCCEEEEEECCCCCCCCCCCHHCCCCHHHHHHHCCC
>Mature Secondary Structure
MKFIKDDFLGGATAASQVEGAYNEGGKTLTLLEMKPFLPIKNRKAIHFGGQTKEEMLDSI
CCCCHHHCCCCCHHHHHHCCHHCCCCCEEEEEEECCCCCCCCCCEEEECCCCHHHHHHHH
ENKQNLHYPKRFGIDFYHRYKEDIALFKEAGMNIYRMSIASRIFPNGDDEKPNEAGIDFY
HCCCCCCCCHHHCCHHHHHHHHHHHHHHHCCCCEEEEHHHHHCCCCCCCCCCCCCCHHHH
RNVFEECKKAGIKVMVTMHHFDTPYEITKKYGGTNRKVIDLFVKYAKTLFEEFDDVVDFL
HHHHHHHHHCCCEEEEEEECCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
PFNEINVAISTEVGLGVFESDFKSKNERDQAAYQGLHHQFIAQAKVIELGKKMCKLKTKF
CCCCEEEEEEECCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GCMVANITTYALDCNPINEIENLKSQQISRFFYDVVAKGEYPTYIKRYFTENNINIIMEN
CEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEEC
DDEKVLKNNTVDFISFSYYMSATVAVQGKDKASGNVSTGGKNPFLEATEGQIDPIGLRVS
CCHHHHHCCCCCEEEEEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEE
LNQMDRYQKPLFISENGIGVLENLDSNNTVKDDYRIDYLGKHFEQINEAIKDGIDVFGYT
HHHHHHHCCCEEEECCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHCCEEE
MTPIDVVSASTNEMSKRYGMIYVDYDDYHNGTGNRFKKKSFNFQNFMKTKEF
ECCHHHCCCCHHHHHHHCCEEEEEECCCCCCCCCCCHHCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7883710; 7704263; 9384377 [H]