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The map label for this gene is argS [H]

Identifier: 50365371

GI number: 50365371

Start: 646410

End: 648071

Strand: Reverse

Name: argS [H]

Synonym: Mfl554

Alternate gene names: 50365371

Gene position: 648071-646410 (Counterclockwise)

Preceding gene: 50365372

Following gene: 50365370

Centisome position: 81.7

GC content: 28.7

Gene sequence:

>1662_bases
ATGGAAAACATAATTAGCATAGTCAAAAAAGACTTAAAAGAAATTGCGCAAAAATTTAATATATCAAAAGACCCAATTGT
TGAAATTAACAAAAATAATATTGATAGTCATTTTTCTACAACTCTTGCTTTAATGAGTGCAAAAGAGTTGAAACAAAATC
CTATTCAATTAGCAGAAAATATTAAAAATGAATTATTACAAAAAGATTACTATGACCAAATAGAAATTGCTGGACCAGGT
TTTATTAACATTAAATTAAAAACAGAATTGCTTTCAACAACAATTAAAAATATTACAACTTTAAAAGAAGCGTACGGTAA
AAATAAAATTAAGAATAAAATTATTAATATTGAATACGTATCAGCTAACCCAACTGGATTTTTACACGTAGGTCATGCAA
GAAATGCGGTAACAGGATCAGTATTAGAAGAGGTTTTGAAATTTGATGGTTATGAAGTTCAGACTGAATTCTACACAAAC
GATGCTGGTAATCAAATAAATATTTTAGCAGTTACTGTTTTTGTTCATTATCTTTGAGCTTTAGGTATTGAAACAGAAAA
ACCTGCTAATACTTATGGTGGAACATTTTATGATGATTTAGCAAATATAATGATTCAAAAATATGGTGATAAATTTAAAA
ATTTAACTTTTACTGAAACAGCTATTTCAGATGAAGAAACTCACCAAATTTTTAGAAAAGAAGCAGTTCAACATTTCTTA
ACTGAAATCAAACAACAATTAAAAGATTTTGGTGTAGTTATTGATCATTATTCAAGTGAACAAGAAATGTATGATACAAA
TCAGATTGAAAAACTTTTAAAAGAATATAAAGAAAAAAATGCTACTTATGAAGCAGATGGAGCTTTATGATTGAAAACAA
CTGAATTTGGGGATGACAAAGATCGTGTTCTTGTTAAAAAAGACGGATCACTAACTTACATTGTTCCTGATCTAGCTACT
CACAACATTAGAATTCAAAGAACTCATGCAGATGTTTTAATTAACATCTGAGGTGGAGATCACCACGGATATATTCCTAG
AATGAGAGCAGGACTACAACTTTTAGGCCATAATCCTGACATTTTAGAAATTGAAATGGTCCAAATGGTAAGGCTTATTA
AAGATGGTAAAGAATACAAAATGAGTAAAAGAAAAGGTACTGCTGTTTGATTAGTTGACATTATGGAAATGGTTGGAAAA
GATGCTTTAAGATATATGTTAGCTTCTAAATCAAGTAGTTCACACATGGATTTAGATTTAGATTTAGTTCAACAAAAAAA
TGCAACAAACCCTGTTTACTATGCTCAATATGCAACAGCAAGATGTCATTCAATTTTAAATCAAGCTGATCAAAAAAATA
TAAAAGCAAATTTAAACGTATCTAACTTATTATCAAACAAAAAAGAAGTTGAGTTATTGTTAACATTAGACAACTTCAAT
CAAGTTATCCAAATGTCTGCAAAAAATAGAGCGCCTCAATTAATATGTGAATACATTCAAACTGTATGTAAACAATTCCA
CTCATACTATGCTGATACAAAAATTCTTGATGAAAATGATGTGCCAACAAGTGAAGCAAGATTGGGATTGGTATTGTCTG
TTTTACAAGTATTGACTAATGCATTTAACATCATTGGTGTCAGTGCTTTAGAAACAATGTAA

Upstream 100 bases:

>100_bases
ATTAGACGAAATATCAAAAGTTAAAGAAAAAGAATTAATGGCAATATAGATTAAAACATTTGTTATACAAATGTTTTTTC
CTTAGAAGGTGGGAATAAAA

Downstream 100 bases:

>100_bases
CATGAATAATTGAGATTTAATTATTGTTGTTCCTTTTGTAATTGTTTTAGGTTTTTTATTCTTATACTTCATTTACAAAA
AACTTAATTTTGAAAAAAAA

Product: arginyl-tRNA synthetase

Products: NA

Alternate protein names: Arginine--tRNA ligase; ArgRS [H]

Number of amino acids: Translated: 553; Mature: 553

Protein sequence:

>553_residues
MENIISIVKKDLKEIAQKFNISKDPIVEINKNNIDSHFSTTLALMSAKELKQNPIQLAENIKNELLQKDYYDQIEIAGPG
FINIKLKTELLSTTIKNITTLKEAYGKNKIKNKIINIEYVSANPTGFLHVGHARNAVTGSVLEEVLKFDGYEVQTEFYTN
DAGNQINILAVTVFVHYLWALGIETEKPANTYGGTFYDDLANIMIQKYGDKFKNLTFTETAISDEETHQIFRKEAVQHFL
TEIKQQLKDFGVVIDHYSSEQEMYDTNQIEKLLKEYKEKNATYEADGALWLKTTEFGDDKDRVLVKKDGSLTYIVPDLAT
HNIRIQRTHADVLINIWGGDHHGYIPRMRAGLQLLGHNPDILEIEMVQMVRLIKDGKEYKMSKRKGTAVWLVDIMEMVGK
DALRYMLASKSSSSHMDLDLDLVQQKNATNPVYYAQYATARCHSILNQADQKNIKANLNVSNLLSNKKEVELLLTLDNFN
QVIQMSAKNRAPQLICEYIQTVCKQFHSYYADTKILDENDVPTSEARLGLVLSVLQVLTNAFNIIGVSALETM

Sequences:

>Translated_553_residues
MENIISIVKKDLKEIAQKFNISKDPIVEINKNNIDSHFSTTLALMSAKELKQNPIQLAENIKNELLQKDYYDQIEIAGPG
FINIKLKTELLSTTIKNITTLKEAYGKNKIKNKIINIEYVSANPTGFLHVGHARNAVTGSVLEEVLKFDGYEVQTEFYTN
DAGNQINILAVTVFVHYL*ALGIETEKPANTYGGTFYDDLANIMIQKYGDKFKNLTFTETAISDEETHQIFRKEAVQHFL
TEIKQQLKDFGVVIDHYSSEQEMYDTNQIEKLLKEYKEKNATYEADGAL*LKTTEFGDDKDRVLVKKDGSLTYIVPDLAT
HNIRIQRTHADVLINI*GGDHHGYIPRMRAGLQLLGHNPDILEIEMVQMVRLIKDGKEYKMSKRKGTAV*LVDIMEMVGK
DALRYMLASKSSSSHMDLDLDLVQQKNATNPVYYAQYATARCHSILNQADQKNIKANLNVSNLLSNKKEVELLLTLDNFN
QVIQMSAKNRAPQLICEYIQTVCKQFHSYYADTKILDENDVPTSEARLGLVLSVLQVLTNAFNIIGVSALETM
>Mature_553_residues
MENIISIVKKDLKEIAQKFNISKDPIVEINKNNIDSHFSTTLALMSAKELKQNPIQLAENIKNELLQKDYYDQIEIAGPG
FINIKLKTELLSTTIKNITTLKEAYGKNKIKNKIINIEYVSANPTGFLHVGHARNAVTGSVLEEVLKFDGYEVQTEFYTN
DAGNQINILAVTVFVHYL*ALGIETEKPANTYGGTFYDDLANIMIQKYGDKFKNLTFTETAISDEETHQIFRKEAVQHFL
TEIKQQLKDFGVVIDHYSSEQEMYDTNQIEKLLKEYKEKNATYEADGAL*LKTTEFGDDKDRVLVKKDGSLTYIVPDLAT
HNIRIQRTHADVLINI*GGDHHGYIPRMRAGLQLLGHNPDILEIEMVQMVRLIKDGKEYKMSKRKGTAV*LVDIMEMVGK
DALRYMLASKSSSSHMDLDLDLVQQKNATNPVYYAQYATARCHSILNQADQKNIKANLNVSNLLSNKKEVELLLTLDNFN
QVIQMSAKNRAPQLICEYIQTVCKQFHSYYADTKILDENDVPTSEARLGLVLSVLQVLTNAFNIIGVSALETM

Specific function: Unknown

COG id: COG0018

COG function: function code J; Arginyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI15149476, Length=557, Percent_Identity=24.4165170556553, Blast_Score=100, Evalue=3e-21,
Organism=Escherichia coli, GI1788184, Length=543, Percent_Identity=24.3093922651934, Blast_Score=118, Evalue=8e-28,
Organism=Saccharomyces cerevisiae, GI6320548, Length=545, Percent_Identity=24.7706422018349, Blast_Score=96, Evalue=1e-20,

Paralogues:

None

Copy number: 789 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR001278
- InterPro:   IPR015945
- InterPro:   IPR005148
- InterPro:   IPR008909
- InterPro:   IPR014729
- InterPro:   IPR009080 [H]

Pfam domain/function: PF03485 Arg_tRNA_synt_N; PF05746 DALR_1; PF00750 tRNA-synt_1d [H]

EC number: =6.1.1.19 [H]

Molecular weight: Translated: 62075; Mature: 62075

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENIISIVKKDLKEIAQKFNISKDPIVEINKNNIDSHFSTTLALMSAKELKQNPIQLAEN
CHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCHHHHHHH
IKNELLQKDYYDQIEIAGPGFINIKLKTELLSTTIKNITTLKEAYGKNKIKNKIINIEYV
HHHHHHHHCCCCCEEECCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCHHHHCEEEEEEEE
SANPTGFLHVGHARNAVTGSVLEEVLKFDGYEVQTEFYTNDAGNQINILAVTVFVHYLAL
ECCCCEEEEECCCCCHHHHHHHHHHHHCCCEEEEEEEEECCCCCEEEEHHHHHHHHHHHH
GIETEKPANTYGGTFYDDLANIMIQKYGDKFKNLTFTETAISDEETHQIFRKEAVQHFLT
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEHHHCCCHHHHHHHHHHHHHHHHH
EIKQQLKDFGVVIDHYSSEQEMYDTNQIEKLLKEYKEKNATYEADGALLKTTEFGDDKDR
HHHHHHHHHCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCEECCCCEEEECCCCCCCCC
VLVKKDGSLTYIVPDLATHNIRIQRTHADVLINIGGDHHGYIPRMRAGLQLLGHNPDILE
EEEEECCCEEEEECCCCCCCEEEEEEEEEEEEECCCCCCCCCHHHHHHHHHHCCCCCEEE
IEMVQMVRLIKDGKEYKMSKRKGTAVLVDIMEMVGKDALRYMLASKSSSSHMDLDLDLVQ
HHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHH
QKNATNPVYYAQYATARCHSILNQADQKNIKANLNVSNLLSNKKEVELLLTLDNFNQVIQ
CCCCCCCEEEEHHHHHHHHHHHHHHHHCCCEECCCHHHHHCCCCEEEEEEEECCHHHHHH
MSAKNRAPQLICEYIQTVCKQFHSYYADTKILDENDVPTSEARLGLVLSVLQVLTNAFNI
HHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHH
IGVSALETM
HHHHHHHCC
>Mature Secondary Structure
MENIISIVKKDLKEIAQKFNISKDPIVEINKNNIDSHFSTTLALMSAKELKQNPIQLAEN
CHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCHHHHHHH
IKNELLQKDYYDQIEIAGPGFINIKLKTELLSTTIKNITTLKEAYGKNKIKNKIINIEYV
HHHHHHHHCCCCCEEECCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCHHHHCEEEEEEEE
SANPTGFLHVGHARNAVTGSVLEEVLKFDGYEVQTEFYTNDAGNQINILAVTVFVHYLAL
ECCCCEEEEECCCCCHHHHHHHHHHHHCCCEEEEEEEEECCCCCEEEEHHHHHHHHHHHH
GIETEKPANTYGGTFYDDLANIMIQKYGDKFKNLTFTETAISDEETHQIFRKEAVQHFLT
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEHHHCCCHHHHHHHHHHHHHHHHH
EIKQQLKDFGVVIDHYSSEQEMYDTNQIEKLLKEYKEKNATYEADGALLKTTEFGDDKDR
HHHHHHHHHCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCEECCCCEEEECCCCCCCCC
VLVKKDGSLTYIVPDLATHNIRIQRTHADVLINIGGDHHGYIPRMRAGLQLLGHNPDILE
EEEEECCCEEEEECCCCCCCEEEEEEEEEEEEECCCCCCCCCHHHHHHHHHHCCCCCEEE
IEMVQMVRLIKDGKEYKMSKRKGTAVLVDIMEMVGKDALRYMLASKSSSSHMDLDLDLVQ
HHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHH
QKNATNPVYYAQYATARCHSILNQADQKNIKANLNVSNLLSNKKEVELLLTLDNFNQVIQ
CCCCCCCEEEEHHHHHHHHHHHHHHHHCCCEECCCHHHHHCCCCEEEEEEEECCHHHHHH
MSAKNRAPQLICEYIQTVCKQFHSYYADTKILDENDVPTSEARLGLVLSVLQVLTNAFNI
HHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHH
IGVSALETM
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA