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The map label for this gene is rluB [H]

Identifier: 50365365

GI number: 50365365

Start: 641228

End: 641983

Strand: Reverse

Name: rluB [H]

Synonym: Mfl548

Alternate gene names: 50365365

Gene position: 641983-641228 (Counterclockwise)

Preceding gene: 50365366

Following gene: 50365364

Centisome position: 80.93

GC content: 27.12

Gene sequence:

>756_bases
ATGGAAAGATTACAGAAAATAATTTCAGCTAGGGGTGTTACTTCAAGAAGAAACGCTGAAAAATTAATAGTTGAGGGAAA
AGTTAAAGTAAATGGTGTAGTTATCACTGAACTTGGTTTTAAAACAAGTACTGATGCTGATATTGAAGTTAACGGTAAAC
AAACAACTAAGAACAATGAAAAGTTTTATTATTTATTTAATAAACCAAGACTTGTTTTAACAACAATGTATGATCCTAAA
CAACGTAAAACAGTTGCAGATTATTTTAAAGACGTACCAACTAGAGTTTATCCTGTTGGAAGACTTGATTATGATGTTAG
TGGCTTAATTATAATGACTAATGATGGTGAATTTGCAAATTTTGTTATGCACCCAAGATATGAATTCTTTAAAACTTATC
AAGGTTTATGTAAAGGTAAAGTTTCAAAACAACAAGTTAACCAATTACTTAAAGGTGTAAAAATTGAAGATGATTACTTT
ACAAAAGCAATTCAGGCAGAATTATTAAATTATGATCAAGAAAAAGATCAATCAATTATTGAAATGACAATTGCAGAAGG
TAAAAAACACCACGTTAAACAAATGTTTTCAGCTATTGAAGCTAACTTAATGAAATTAAAAAGAACTAAAATTGAATTTT
TGGAAGTTGATGATTTAGAAGTTGGAAGATATAGAGAATTAAAACCTCATGAGGTTAAAAAATTCTATGGAATTTATCAA
TCAACAAAAAGAAAAGAAGACTTAAAAATTAAATAA

Upstream 100 bases:

>100_bases
AAGATGAAGAAAACAATTTTGAAGAAGAAATTAAAGTTACTGAAGAAATGATCTCAGAAGAAGATGTTTTTGAGGAAGAT
GACTCAGAAGGAATGTTTTA

Downstream 100 bases:

>100_bases
AGAGGTATATATTATGAAAATAGCAATTTTTGGAACAGTAGGAGCAGGTAAATCATCAATATCACAAGAATTATCAGAAA
GATTAAATTATGAAATTTTT

Product: 23S rRNA pseudouridine synthase

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B [H]

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MERLQKIISARGVTSRRNAEKLIVEGKVKVNGVVITELGFKTSTDADIEVNGKQTTKNNEKFYYLFNKPRLVLTTMYDPK
QRKTVADYFKDVPTRVYPVGRLDYDVSGLIIMTNDGEFANFVMHPRYEFFKTYQGLCKGKVSKQQVNQLLKGVKIEDDYF
TKAIQAELLNYDQEKDQSIIEMTIAEGKKHHVKQMFSAIEANLMKLKRTKIEFLEVDDLEVGRYRELKPHEVKKFYGIYQ
STKRKEDLKIK

Sequences:

>Translated_251_residues
MERLQKIISARGVTSRRNAEKLIVEGKVKVNGVVITELGFKTSTDADIEVNGKQTTKNNEKFYYLFNKPRLVLTTMYDPK
QRKTVADYFKDVPTRVYPVGRLDYDVSGLIIMTNDGEFANFVMHPRYEFFKTYQGLCKGKVSKQQVNQLLKGVKIEDDYF
TKAIQAELLNYDQEKDQSIIEMTIAEGKKHHVKQMFSAIEANLMKLKRTKIEFLEVDDLEVGRYRELKPHEVKKFYGIYQ
STKRKEDLKIK
>Mature_251_residues
MERLQKIISARGVTSRRNAEKLIVEGKVKVNGVVITELGFKTSTDADIEVNGKQTTKNNEKFYYLFNKPRLVLTTMYDPK
QRKTVADYFKDVPTRVYPVGRLDYDVSGLIIMTNDGEFANFVMHPRYEFFKTYQGLCKGKVSKQQVNQLLKGVKIEDDYF
TKAIQAELLNYDQEKDQSIIEMTIAEGKKHHVKQMFSAIEANLMKLKRTKIEFLEVDDLEVGRYRELKPHEVKKFYGIYQ
STKRKEDLKIK

Specific function: Responsible for synthesis of pseudouridine from uracil- 2633 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=236, Percent_Identity=30.0847457627119, Blast_Score=117, Evalue=7e-28,
Organism=Escherichia coli, GI1787524, Length=217, Percent_Identity=30.4147465437788, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1790453, Length=242, Percent_Identity=25.2066115702479, Blast_Score=80, Evalue=9e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 29286; Mature: 29286

Theoretical pI: Translated: 9.92; Mature: 9.92

Prosite motif: PS50889 S4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERLQKIISARGVTSRRNAEKLIVEGKVKVNGVVITELGFKTSTDADIEVNGKQTTKNNE
CHHHHHHHHHCCCCCCCCCCEEEEECEEEEEEEEEEEECCCCCCCCCEEECCCCCCCCCC
KFYYLFNKPRLVLTTMYDPKQRKTVADYFKDVPTRVYPVGRLDYDVSGLIIMTNDGEFAN
EEEEEECCCEEEEEECCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEECCCCEEE
FVMHPRYEFFKTYQGLCKGKVSKQQVNQLLKGVKIEDDYFTKAIQAELLNYDQEKDQSII
EEECCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCHHHE
EMTIAEGKKHHVKQMFSAIEANLMKLKRTKIEFLEVDDLEVGRYRELKPHEVKKFYGIYQ
EHHHHCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCHHHHHHHHHHHH
STKRKEDLKIK
HCCCCCCCCCC
>Mature Secondary Structure
MERLQKIISARGVTSRRNAEKLIVEGKVKVNGVVITELGFKTSTDADIEVNGKQTTKNNE
CHHHHHHHHHCCCCCCCCCCEEEEECEEEEEEEEEEEECCCCCCCCCEEECCCCCCCCCC
KFYYLFNKPRLVLTTMYDPKQRKTVADYFKDVPTRVYPVGRLDYDVSGLIIMTNDGEFAN
EEEEEECCCEEEEEECCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEECCCCEEE
FVMHPRYEFFKTYQGLCKGKVSKQQVNQLLKGVKIEDDYFTKAIQAELLNYDQEKDQSII
EEECCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCHHHE
EMTIAEGKKHHVKQMFSAIEANLMKLKRTKIEFLEVDDLEVGRYRELKPHEVKKFYGIYQ
EHHHHCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCHHHHHHHHHHHH
STKRKEDLKIK
HCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934829; 9384377; 9888802 [H]