Click here to switch to the map view.

The map label for this gene is trmD [H]

Identifier: 50365357

GI number: 50365357

Start: 635775

End: 636506

Strand: Reverse

Name: trmD [H]

Synonym: Mfl540

Alternate gene names: 50365357

Gene position: 636506-635775 (Counterclockwise)

Preceding gene: 50365358

Following gene: 50365356

Centisome position: 80.24

GC content: 28.28

Gene sequence:

>732_bases
ATGAAATATTCAATTCTAACGTTATTCCCAAATATTATTAAATCTTATATTTCTGAATCAATTATTAAAAAAGCTCTAGA
GCGTGAAAAGATTGAATTAGAAATTATTGATATAAGAGATTTTACAACACTACCTCATAATCAAGTTGATGAATATCAAT
TTGGAGGGGGAAGAGGAATGGTTTTAATGTGTGATCCAGTTGTTAGTGCAATTGAATCTGTTAAAACAGAAAACTCAATT
GTTGTATTAACATCTCCACAAGGGAAAACTTGAAATCAAAACTTAGCAAAATCATTTGCAACAGAATACAATCACATCAT
TATAGTATGTGGTCATTATGAAGGTTTTGATGAAAGAATATTAAAATATATTGATGTTGAAATATCTATTGGTGACTATG
TGTTAACTGGTGGGGAGTTACCTTCATTAATAATGCTAGATTCTATTACTAGAATTCTACCAGGAGTTATAAATACTGAA
TCACATGAGCAAGAAAGTTTTGAAAATAATTTATTAGATTATCCAGTGTATACAAAACCCCATGATTATAGGGGAGACAA
AGTTCCTGAAGTCCTTTTAAGTGGACATCACGCTAATATTCAAAAGTGAAGAGACGAACAACAATTAATTTCTACTTTTA
ATAAAAGACCAGATTTAATTGATGAGTCCAAATTAAATAAAATACAAATACAATTATTAGAAAATCTTAGAAAATCGAAA
GGAGATAAATAA

Upstream 100 bases:

>100_bases
AAGAAAAAGCATTTTGAGTACCTTTGGTTGATGTGTACACTAACAACATTGATGATGAATCTAGAATTATAACTTTAAAA
AACATTGAAGGATTAAAATA

Downstream 100 bases:

>100_bases
TATGGCATCAAAAGCAACTAAAACAACACAATCAAAATACGCTATTATTAACAACCAATTACGTAACGACTTACCAGATT
TTACATCAGGGGATACTATT

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase [H]

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MKYSILTLFPNIIKSYISESIIKKALEREKIELEIIDIRDFTTLPHNQVDEYQFGGGRGMVLMCDPVVSAIESVKTENSI
VVLTSPQGKTWNQNLAKSFATEYNHIIIVCGHYEGFDERILKYIDVEISIGDYVLTGGELPSLIMLDSITRILPGVINTE
SHEQESFENNLLDYPVYTKPHDYRGDKVPEVLLSGHHANIQKWRDEQQLISTFNKRPDLIDESKLNKIQIQLLENLRKSK
GDK

Sequences:

>Translated_243_residues
MKYSILTLFPNIIKSYISESIIKKALEREKIELEIIDIRDFTTLPHNQVDEYQFGGGRGMVLMCDPVVSAIESVKTENSI
VVLTSPQGKT*NQNLAKSFATEYNHIIIVCGHYEGFDERILKYIDVEISIGDYVLTGGELPSLIMLDSITRILPGVINTE
SHEQESFENNLLDYPVYTKPHDYRGDKVPEVLLSGHHANIQK*RDEQQLISTFNKRPDLIDESKLNKIQIQLLENLRKSK
GDK
>Mature_243_residues
MKYSILTLFPNIIKSYISESIIKKALEREKIELEIIDIRDFTTLPHNQVDEYQFGGGRGMVLMCDPVVSAIESVKTENSI
VVLTSPQGKT*NQNLAKSFATEYNHIIIVCGHYEGFDERILKYIDVEISIGDYVLTGGELPSLIMLDSITRILPGVINTE
SHEQESFENNLLDYPVYTKPHDYRGDKVPEVLLSGHHANIQK*RDEQQLISTFNKRPDLIDESKLNKIQIQLLENLRKSK
GDK

Specific function: Specifically methylates guanosine-37 in various tRNAs [H]

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family [H]

Homologues:

Organism=Escherichia coli, GI1788959, Length=241, Percent_Identity=38.1742738589212, Blast_Score=165, Evalue=2e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148 [H]

Pfam domain/function: PF01746 tRNA_m1G_MT [H]

EC number: =2.1.1.31 [H]

Molecular weight: Translated: 27429; Mature: 27429

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYSILTLFPNIIKSYISESIIKKALEREKIELEIIDIRDFTTLPHNQVDEYQFGGGRGM
CCEEHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCCCCCEEECCCCEE
VLMCDPVVSAIESVKTENSIVVLTSPQGKTNQNLAKSFATEYNHIIIVCGHYEGFDERIL
EEEECHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHCCCEEEEEEECCCCHHHHHH
KYIDVEISIGDYVLTGGELPSLIMLDSITRILPGVINTESHEQESFENNLLDYPVYTKPH
HHHEEEEEECCEEEECCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCCCCC
DYRGDKVPEVLLSGHHANIQKRDEQQLISTFNKRPDLIDESKLNKIQIQLLENLRKSKGD
CCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
K
C
>Mature Secondary Structure
MKYSILTLFPNIIKSYISESIIKKALEREKIELEIIDIRDFTTLPHNQVDEYQFGGGRGM
CCEEHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCCCCCEEECCCCEE
VLMCDPVVSAIESVKTENSIVVLTSPQGKTNQNLAKSFATEYNHIIIVCGHYEGFDERIL
EEEECHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHCCCEEEEEEECCCCHHHHHH
KYIDVEISIGDYVLTGGELPSLIMLDSITRILPGVINTESHEQESFENNLLDYPVYTKPH
HHHEEEEEECCEEEECCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCCCCC
DYRGDKVPEVLLSGHHANIQKRDEQQLISTFNKRPDLIDESKLNKIQIQLLENLRKSKGD
CCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
K
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA