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The map label for this gene is scrA [H]

Identifier: 50365344

GI number: 50365344

Start: 620061

End: 621593

Strand: Reverse

Name: scrA [H]

Synonym: Mfl527

Alternate gene names: 50365344

Gene position: 621593-620061 (Counterclockwise)

Preceding gene: 50365345

Following gene: 50365343

Centisome position: 78.36

GC content: 28.83

Gene sequence:

>1533_bases
ATGAAACATGCAAAAATAAACAGCCACTTTTACGACGAATGTAAAAAAGTAAGAGAAGCTGTCGGTGGAATAAAAAACAT
CGAGTTAATTAATAGATGTTCAACAAGAATCAGAATCAAAGTTATTGACATAACAAAATTAAATTTGAATAAACTTGAAG
GTTCTAAAATTTTTGTTAAAACATTATTAAAAGATGACTTTGTTCAATTGATTGTTAACGAGAATATTGATGAAGTTTTC
TTAAATTTATTAAAATCGCTAAAAATTTCATATGAAGAGATTCCTAACGATTTTGAAGTAAGAGTTAATAAAGAGGGGAA
CTTTTTTAAAGTAGTAACTGATGGAATTTCTGTTATTGTAAAACCTCTTATACCTCTTTTGATAACAATGGCCATAGTAG
CTACATTATCAAATGTATTTAATGGTATAGATTTTGGATCAGGAACTTTATCAGAAACTGGTCAATTTGCAAAAGCAGTT
GGGGAAATGTTTGATATTTTACAAAAAGCATTAAACTTAGCTTTCTCAATAATGATTCCTTGATCAATTTTTAAACTTAT
GAAAGGTAGTCAAGCAATTGGTATATCAATTGGTATTGTATTATGTTTTCATGGTTTGATAAGCACAAATGACATTATGA
GTGGAGAATATGGTGGCATATTTAAATGATTTGGAGATGCATCATTCCTTGAATCAGGATATCCTTGAAAAATATCATAT
GTTGGTCAAATACTTCCTATAGTTGCGATGAGTTTTATTGCAGTCTATATTGAAAGATTTGCAAACAAATTTGATATACC
TGTATTTAAAGAAATGGTTGCAATACCAATGATTACAATTTCTACATTCTTTATTGGTATTTTATTGGTAGGTCCATTAG
GCTTATTATTAACATATGGAATGAATGAAGGTGTGGTTTGAGCAACTACAGATAATGTTGCTAAATATATATTTAATCCA
ATTTTAGGATTAGCCTTACCTTGGTTAGTTATTACCGGATTAATTCAAGTTTTGGTAGTTATTAACTTACAACAGTTTAC
TACATTTGGTGGAACAACAATGATGCCGATGTTTACACAATTAAATATTGCAGTAGCAACTTCAATCTTAGCTGTAACAA
TTATAAATAGACATAATAAAGAATTAAAAAGAACAGCAATACCTTCATATTCATTAGCATATGTAAGTGGATCGACTGAA
CCAGCATTATTTGGTGTAGGATTAAAATTTGTTTATCCAGTTATTGCAGCAAGTATTGGTGCAACTGTTGGTATTATAAT
AACAACATTCGCTGGAGTGATTTGTACAAATGGTAATGCTTCATTGTTAGTATTTTTATCAATTACAACTAAACCTGAAA
TATTAGATAAATTTAACATACACACAATAGCTGGTGGGCCATACTTATGAATGGCAATAGCTATTGTGGCAACATTTATA
ACAACTTTCTTTGCAACAATTGGTTTATCAAAAATTAAAGTTTTCAAAGAAATGAATGCAAAAGTTTTAGAAAGAGATTT
TTCGGTGAATTAA

Upstream 100 bases:

>100_bases
AACAACTTATTGTAGGATAAGTTAGACTTATAATAAGTTTAGAATAATTTCTAAAATGTTGAAAAGAAATAGAAATTATT
ATTATTTGAAAGGTGAAAAA

Downstream 100 bases:

>100_bases
CATGAAATTAAATAAAGAAACAATATTAAATTTAATAACAGAACATAATCAAGATGATATTAAAAAAGCAAATGAATTAG
TAGCAAGTGATAAATATTAT

Product: sucrose PTS system IIBC component

Products: NA

Alternate protein names: EIIBC-Scr; EII-Scr; Sucrose-specific phosphotransferase enzyme IIB component; PTS system sucrose-specific EIIB component; Sucrose permease IIC component; PTS system sucrose-specific EIIC component [H]

Number of amino acids: Translated: 510; Mature: 510

Protein sequence:

>510_residues
MKHAKINSHFYDECKKVREAVGGIKNIELINRCSTRIRIKVIDITKLNLNKLEGSKIFVKTLLKDDFVQLIVNENIDEVF
LNLLKSLKISYEEIPNDFEVRVNKEGNFFKVVTDGISVIVKPLIPLLITMAIVATLSNVFNGIDFGSGTLSETGQFAKAV
GEMFDILQKALNLAFSIMIPWSIFKLMKGSQAIGISIGIVLCFHGLISTNDIMSGEYGGIFKWFGDASFLESGYPWKISY
VGQILPIVAMSFIAVYIERFANKFDIPVFKEMVAIPMITISTFFIGILLVGPLGLLLTYGMNEGVVWATTDNVAKYIFNP
ILGLALPWLVITGLIQVLVVINLQQFTTFGGTTMMPMFTQLNIAVATSILAVTIINRHNKELKRTAIPSYSLAYVSGSTE
PALFGVGLKFVYPVIAASIGATVGIIITTFAGVICTNGNASLLVFLSITTKPEILDKFNIHTIAGGPYLWMAIAIVATFI
TTFFATIGLSKIKVFKEMNAKVLERDFSVN

Sequences:

>Translated_510_residues
MKHAKINSHFYDECKKVREAVGGIKNIELINRCSTRIRIKVIDITKLNLNKLEGSKIFVKTLLKDDFVQLIVNENIDEVF
LNLLKSLKISYEEIPNDFEVRVNKEGNFFKVVTDGISVIVKPLIPLLITMAIVATLSNVFNGIDFGSGTLSETGQFAKAV
GEMFDILQKALNLAFSIMIP*SIFKLMKGSQAIGISIGIVLCFHGLISTNDIMSGEYGGIFK*FGDASFLESGYP*KISY
VGQILPIVAMSFIAVYIERFANKFDIPVFKEMVAIPMITISTFFIGILLVGPLGLLLTYGMNEGVV*ATTDNVAKYIFNP
ILGLALPWLVITGLIQVLVVINLQQFTTFGGTTMMPMFTQLNIAVATSILAVTIINRHNKELKRTAIPSYSLAYVSGSTE
PALFGVGLKFVYPVIAASIGATVGIIITTFAGVICTNGNASLLVFLSITTKPEILDKFNIHTIAGGPYL*MAIAIVATFI
TTFFATIGLSKIKVFKEMNAKVLERDFSVN
>Mature_510_residues
MKHAKINSHFYDECKKVREAVGGIKNIELINRCSTRIRIKVIDITKLNLNKLEGSKIFVKTLLKDDFVQLIVNENIDEVF
LNLLKSLKISYEEIPNDFEVRVNKEGNFFKVVTDGISVIVKPLIPLLITMAIVATLSNVFNGIDFGSGTLSETGQFAKAV
GEMFDILQKALNLAFSIMIP*SIFKLMKGSQAIGISIGIVLCFHGLISTNDIMSGEYGGIFK*FGDASFLESGYP*KISY
VGQILPIVAMSFIAVYIERFANKFDIPVFKEMVAIPMITISTFFIGILLVGPLGLLLTYGMNEGVV*ATTDNVAKYIFNP
ILGLALPWLVITGLIQVLVVINLQQFTTFGGTTMMPMFTQLNIAVATSILAVTIINRHNKELKRTAIPSYSLAYVSGSTE
PALFGVGLKFVYPVIAASIGATVGIIITTFAGVICTNGNASLLVFLSITTKPEILDKFNIHTIAGGPYL*MAIAIVATFI
TTFFATIGLSKIKVFKEMNAKVLERDFSVN

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1263

COG function: function code G; Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI2367362, Length=441, Percent_Identity=23.8095238095238, Blast_Score=108, Evalue=8e-25,
Organism=Escherichia coli, GI48994906, Length=487, Percent_Identity=24.0246406570842, Blast_Score=87, Evalue=4e-18,
Organism=Escherichia coli, GI1790159, Length=473, Percent_Identity=24.3128964059197, Blast_Score=80, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018113
- InterPro:   IPR001996
- InterPro:   IPR003352
- InterPro:   IPR013013
- InterPro:   IPR011535
- InterPro:   IPR010973 [H]

Pfam domain/function: PF00367 PTS_EIIB; PF02378 PTS_EIIC [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 55096; Mature: 55096

Theoretical pI: Translated: 9.15; Mature: 9.15

Prosite motif: PS01035 PTS_EIIB_TYPE_1_CYS ; PS51098 PTS_EIIB_TYPE_1 ; PS51103 PTS_EIIC_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHAKINSHFYDECKKVREAVGGIKNIELINRCSTRIRIKVIDITKLNLNKLEGSKIFVK
CCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEEEEEEEEEEECCCCCCCCHHHHH
TLLKDDFVQLIVNENIDEVFLNLLKSLKISYEEIPNDFEVRVNKEGNFFKVVTDGISVIV
HHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHCCCCEEEEECCCCCEEEEEHHHHHHHH
KPLIPLLITMAIVATLSNVFNGIDFGSGTLSETGQFAKAVGEMFDILQKALNLAFSIMIP
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SIFKLMKGSQAIGISIGIVLCFHGLISTNDIMSGEYGGIFKFGDASFLESGYPKISYVGQ
HHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCHHHHHCCCCCHHHHHH
ILPIVAMSFIAVYIERFANKFDIPVFKEMVAIPMITISTFFIGILLVGPLGLLLTYGMNE
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GVVATTDNVAKYIFNPILGLALPWLVITGLIQVLVVINLQQFTTFGGTTMMPMFTQLNIA
CEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
VATSILAVTIINRHNKELKRTAIPSYSLAYVSGSTEPALFGVGLKFVYPVIAASIGATVG
HHHHHHHHHHHHHCCHHHHHHCCCCCEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHH
IIITTFAGVICTNGNASLLVFLSITTKPEILDKFNIHTIAGGPYLMAIAIVATFITTFFA
HHHHHHHHHEEECCCCEEEEEEEECCCHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHH
TIGLSKIKVFKEMNAKVLERDFSVN
HHHHHHHHHHHHHCHHHHHHCCCCC
>Mature Secondary Structure
MKHAKINSHFYDECKKVREAVGGIKNIELINRCSTRIRIKVIDITKLNLNKLEGSKIFVK
CCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEEEEEEEEEEECCCCCCCCHHHHH
TLLKDDFVQLIVNENIDEVFLNLLKSLKISYEEIPNDFEVRVNKEGNFFKVVTDGISVIV
HHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHCCCCEEEEECCCCCEEEEEHHHHHHHH
KPLIPLLITMAIVATLSNVFNGIDFGSGTLSETGQFAKAVGEMFDILQKALNLAFSIMIP
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SIFKLMKGSQAIGISIGIVLCFHGLISTNDIMSGEYGGIFKFGDASFLESGYPKISYVGQ
HHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCHHHHHCCCCCHHHHHH
ILPIVAMSFIAVYIERFANKFDIPVFKEMVAIPMITISTFFIGILLVGPLGLLLTYGMNE
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GVVATTDNVAKYIFNPILGLALPWLVITGLIQVLVVINLQQFTTFGGTTMMPMFTQLNIA
CEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
VATSILAVTIINRHNKELKRTAIPSYSLAYVSGSTEPALFGVGLKFVYPVIAASIGATVG
HHHHHHHHHHHHHCCHHHHHHCCCCCEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHH
IIITTFAGVICTNGNASLLVFLSITTKPEILDKFNIHTIAGGPYLMAIAIVATFITTFFA
HHHHHHHHHEEECCCCEEEEEEEECCCHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHH
TIGLSKIKVFKEMNAKVLERDFSVN
HHHHHHHHHHHHHCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8232209 [H]