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The map label for this gene is nrdE [H]

Identifier: 50365345

GI number: 50365345

Start: 621769

End: 623934

Strand: Reverse

Name: nrdE [H]

Synonym: Mfl528

Alternate gene names: 50365345

Gene position: 623934-621769 (Counterclockwise)

Preceding gene: 50365346

Following gene: 50365344

Centisome position: 78.66

GC content: 31.21

Gene sequence:

>2166_bases
ATGGAAGATAAAAAAATAAATACATTATCTGGAACAGATGAAAGTGACGAATATGTTACTTTAAATGCCAGAGCTAAAAT
ATTAGCTGCTGGATCAGATAACTTCAAATTAGATGTTGATGCTGCTAAAGCTTATTTAAAACATCATGTTGAACCTAGAT
TAGTAAAATTTGGTTCAGTTAAAGAAAGAATAGAATACCTAGTTTCTAATGGATACTACGAAGAAGAACTTATAAATAAA
TATACAATAGAACAAATTGAAGAAATTACTGATATTGCTTATGGATTTCACCGTGAATTCCCAAGTTTTATGGGAGCTAT
GAAATTTTACAATTCATATGGTTTAAAAACTTTTGATGGACAAAAATATTTAGAAAATTTTGAGGATAGAGCAGTAATGA
ATGCTTTATTCTTAGGAAATGGTAATTTTGAATCTGCAACAAAAACATTAAGACAATTAATGCTGGGTAGATTTCAACCA
GCTACACCTACATTCTTAAATGCAGGTAAAAAACAACGTGGTGAATATGTTTCTTGTTACTTATTAAGAGTAGAAGACAA
CATGGAATCAATTTGTCGTGCTGTTACAACATCACTACAATTATCAAAACGTGGTGGTGGAGTTGCTTTATGTTTAACTA
ATCTACGTGAATTAGGCGCACCAATAAAACAAATCGCTAACCAAGCAACTGGTGTTATCCCAGTTATGAAAATATTAGAA
GATTCATTCTCATATGCAAATCAATTAGGTCAAAGACAAGGTGCTGGAGCTGTTTATTTAAATGCTCACCACCCAGATGT
TATGTCATTCTTAGATACTAAAAGAGAAAATGCTGATGAAAAAATCCGTATTAAATCATTATCACTAGGATTAGTTATTC
CAGATATTACTTTTGAATTAGCTCAAAAAAATGAAGAAATGGCTTTATTTAGTCCTTATGATGTTGAAAGAGAATATGGA
AAAGCAATGTCAGATATTTCAATTACTCAAGAGTATCAAAATCTAGTTAAAAACCCTAATATTAAAAAAACTTATATTAA
TGCGAGAAAATTCTTTTTAACTGTTGCTGAATTACACTTTGAAAGTGGTTACCCATACATCTTATTTGATGATACAGTAA
ACAACAATAATGCTCACGCAGCAACTGGAAGAATTGTTATGAGTAACTTATGTAGTGAAATTGCACAAGTTTCAACACCA
AGTACATTTAACGATGATTTAAGTTTCAAAAGCATGGGAGACGATATCTGTTGTAATTTAGGAAGTCTTAACATTGCTAA
AGTAATGGAAAGTGGAGCTGAATTTGCAGAAGTTATTGAACACTCAATTAATGCTTTAGATCTAGTTTCAAGAACAAGTG
ATTTAAGTAGTGCTCCTTCAATTCAACAAGGAAACAAAAAAAATCATGCTGTAGGATTAGGTGCTATGAACTTACATGGT
TTCTTAGCTACAAACTTCATTTGATATAACTCAATTGAAGCGGTTGATTTTACAAACATGTTCTTCTATGCAATGGCATA
TAACGCATTTAAAGCATCTCACAAATTAGCTTTACAATATGGCACATTTGATGGATTTGAAACATCACAATTTGCTGATG
GAAGTTGATTTAACAAATATACTAAATGCGCAGAAGATAAATGAACTCCTGAAACTCAAAGAGTTAGAGAATTATTTGCT
CAATATAATGTAACTATTCCAACTCAAAAAGATTGAATTGAATTAGTTGAACTAATTAAAAAAGATGGAATTGCTAACTC
ACATTTAATGGCTGTTGCTCCTACAGGATCAATTAGTTACTTATCTTCATGTACTCCAAGTTTACAACCAGTTGTAGCAC
CAATTGAAGTACGTAAAGAAGGAAAATTAGGAAGAATTTATGTTCCAGCATACAACATTAATTTTGATAACATGGCTTAC
TATGCTTTAGGAGCATATGAAGTTGGACCAATGCCAATTATTGATATTGTTGCAGCAGCTCAACAACACGTTGATCAAGC
TATATCATTAACATTATTTATGACAGACCAAGTTACAACTCGTGACTTAAACAAAGCATACATTCATGCATTTAAGAAAA
AATGTGCATCAATCTACTACGTACGTGTAAGACAAGAAGTATTAGAAGATAGTGAAAACTATGAATGCGACGCATGTGTA
ATTTAA

Upstream 100 bases:

>100_bases
AACTTAATGTTCCATTGTTGTATCAATTTGAATTATTAGGAACTCAAAACGATGTAGAAAAAATCAATGAAATATTGAAA
GAATTTTGAGGGAAATAAAA

Downstream 100 bases:

>100_bases
TAAAAAACTAAGTGGTTATTAGCCACTTTTTTTGTTTGCATTTTATCCAACTTATAATTATAGGTTTTTACTTTTAACAA
CTTATTGTAGGATAAGTTAG

Product: ribonucleotide-diphosphate reductase subunit alpha

Products: NA

Alternate protein names: Ribonucleotide reductase [H]

Number of amino acids: Translated: 721; Mature: 721

Protein sequence:

>721_residues
MEDKKINTLSGTDESDEYVTLNARAKILAAGSDNFKLDVDAAKAYLKHHVEPRLVKFGSVKERIEYLVSNGYYEEELINK
YTIEQIEEITDIAYGFHREFPSFMGAMKFYNSYGLKTFDGQKYLENFEDRAVMNALFLGNGNFESATKTLRQLMLGRFQP
ATPTFLNAGKKQRGEYVSCYLLRVEDNMESICRAVTTSLQLSKRGGGVALCLTNLRELGAPIKQIANQATGVIPVMKILE
DSFSYANQLGQRQGAGAVYLNAHHPDVMSFLDTKRENADEKIRIKSLSLGLVIPDITFELAQKNEEMALFSPYDVEREYG
KAMSDISITQEYQNLVKNPNIKKTYINARKFFLTVAELHFESGYPYILFDDTVNNNNAHAATGRIVMSNLCSEIAQVSTP
STFNDDLSFKSMGDDICCNLGSLNIAKVMESGAEFAEVIEHSINALDLVSRTSDLSSAPSIQQGNKKNHAVGLGAMNLHG
FLATNFIWYNSIEAVDFTNMFFYAMAYNAFKASHKLALQYGTFDGFETSQFADGSWFNKYTKCAEDKWTPETQRVRELFA
QYNVTIPTQKDWIELVELIKKDGIANSHLMAVAPTGSISYLSSCTPSLQPVVAPIEVRKEGKLGRIYVPAYNINFDNMAY
YALGAYEVGPMPIIDIVAAAQQHVDQAISLTLFMTDQVTTRDLNKAYIHAFKKKCASIYYVRVRQEVLEDSENYECDACV
I

Sequences:

>Translated_721_residues
MEDKKINTLSGTDESDEYVTLNARAKILAAGSDNFKLDVDAAKAYLKHHVEPRLVKFGSVKERIEYLVSNGYYEEELINK
YTIEQIEEITDIAYGFHREFPSFMGAMKFYNSYGLKTFDGQKYLENFEDRAVMNALFLGNGNFESATKTLRQLMLGRFQP
ATPTFLNAGKKQRGEYVSCYLLRVEDNMESICRAVTTSLQLSKRGGGVALCLTNLRELGAPIKQIANQATGVIPVMKILE
DSFSYANQLGQRQGAGAVYLNAHHPDVMSFLDTKRENADEKIRIKSLSLGLVIPDITFELAQKNEEMALFSPYDVEREYG
KAMSDISITQEYQNLVKNPNIKKTYINARKFFLTVAELHFESGYPYILFDDTVNNNNAHAATGRIVMSNLCSEIAQVSTP
STFNDDLSFKSMGDDICCNLGSLNIAKVMESGAEFAEVIEHSINALDLVSRTSDLSSAPSIQQGNKKNHAVGLGAMNLHG
FLATNFI*YNSIEAVDFTNMFFYAMAYNAFKASHKLALQYGTFDGFETSQFADGS*FNKYTKCAEDK*TPETQRVRELFA
QYNVTIPTQKD*IELVELIKKDGIANSHLMAVAPTGSISYLSSCTPSLQPVVAPIEVRKEGKLGRIYVPAYNINFDNMAY
YALGAYEVGPMPIIDIVAAAQQHVDQAISLTLFMTDQVTTRDLNKAYIHAFKKKCASIYYVRVRQEVLEDSENYECDACV
I
>Mature_721_residues
MEDKKINTLSGTDESDEYVTLNARAKILAAGSDNFKLDVDAAKAYLKHHVEPRLVKFGSVKERIEYLVSNGYYEEELINK
YTIEQIEEITDIAYGFHREFPSFMGAMKFYNSYGLKTFDGQKYLENFEDRAVMNALFLGNGNFESATKTLRQLMLGRFQP
ATPTFLNAGKKQRGEYVSCYLLRVEDNMESICRAVTTSLQLSKRGGGVALCLTNLRELGAPIKQIANQATGVIPVMKILE
DSFSYANQLGQRQGAGAVYLNAHHPDVMSFLDTKRENADEKIRIKSLSLGLVIPDITFELAQKNEEMALFSPYDVEREYG
KAMSDISITQEYQNLVKNPNIKKTYINARKFFLTVAELHFESGYPYILFDDTVNNNNAHAATGRIVMSNLCSEIAQVSTP
STFNDDLSFKSMGDDICCNLGSLNIAKVMESGAEFAEVIEHSINALDLVSRTSDLSSAPSIQQGNKKNHAVGLGAMNLHG
FLATNFI*YNSIEAVDFTNMFFYAMAYNAFKASHKLALQYGTFDGFETSQFADGS*FNKYTKCAEDK*TPETQRVRELFA
QYNVTIPTQKD*IELVELIKKDGIANSHLMAVAPTGSISYLSSCTPSLQPVVAPIEVRKEGKLGRIYVPAYNINFDNMAY
YALGAYEVGPMPIIDIVAAAQQHVDQAISLTLFMTDQVTTRDLNKAYIHAFKKKCASIYYVRVRQEVLEDSENYECDACV
I

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0209

COG function: function code F; Ribonucleotide reductase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase large chain family [H]

Homologues:

Organism=Homo sapiens, GI4506749, Length=524, Percent_Identity=25.1908396946565, Blast_Score=170, Evalue=5e-42,
Organism=Escherichia coli, GI1789030, Length=711, Percent_Identity=53.4458509142053, Blast_Score=756, Evalue=0.0,
Organism=Escherichia coli, GI2367133, Length=683, Percent_Identity=24.8901903367496, Blast_Score=143, Evalue=5e-35,
Organism=Caenorhabditis elegans, GI17555242, Length=525, Percent_Identity=25.9047619047619, Blast_Score=160, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6322123, Length=649, Percent_Identity=24.3451463790447, Blast_Score=164, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6320914, Length=659, Percent_Identity=24.5827010622155, Blast_Score=159, Evalue=2e-39,
Organism=Drosophila melanogaster, GI17352481, Length=526, Percent_Identity=25.8555133079848, Blast_Score=155, Evalue=9e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013346
- InterPro:   IPR013509
- InterPro:   IPR000788
- InterPro:   IPR008926
- InterPro:   IPR013554 [H]

Pfam domain/function: PF02867 Ribonuc_red_lgC; PF00317 Ribonuc_red_lgN; PF08343 RNR_N [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 80100; Mature: 80100

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEDKKINTLSGTDESDEYVTLNARAKILAAGSDNFKLDVDAAKAYLKHHVEPRLVKFGSV
CCCCCCCCCCCCCCCCCEEEEECCEEEEEECCCCEEEEHHHHHHHHHHCCCCHHHHHCCH
KERIEYLVSNGYYEEELINKYTIEQIEEITDIAYGFHREFPSFMGAMKFYNSYGLKTFDG
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
QKYLENFEDRAVMNALFLGNGNFESATKTLRQLMLGRFQPATPTFLNAGKKQRGEYVSCY
HHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHCCCCCCCCHHHCCCCHHCCCEEEEE
LLRVEDNMESICRAVTTSLQLSKRGGGVALCLTNLRELGAPIKQIANQATGVIPVMKILE
EEEECCCHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHCCHHHHHHHHHCCHHHHHHHHH
DSFSYANQLGQRQGAGAVYLNAHHPDVMSFLDTKRENADEKIRIKSLSLGLVIPDITFEL
HHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCCCCCEEEEEEEEEEEEECCCHHHH
AQKNEEMALFSPYDVEREYGKAMSDISITQEYQNLVKNPNIKKTYINARKFFLTVAELHF
HCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHH
ESGYPYILFDDTVNNNNAHAATGRIVMSNLCSEIAQVSTPSTFNDDLSFKSMGDDICCNL
CCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCHHHCCC
GSLNIAKVMESGAEFAEVIEHSINALDLVSRTSDLSSAPSIQQGNKKNHAVGLGAMNLHG
CCCHHHHHHHCCHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECHHHHH
FLATNFIYNSIEAVDFTNMFFYAMAYNAFKASHKLALQYGTFDGFETSQFADGSFNKYTK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCHHHHH
CAEDKTPETQRVRELFAQYNVTIPTQKDIELVELIKKDGIANSHLMAVAPTGSISYLSSC
HCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHC
TPSLQPVVAPIEVRKEGKLGRIYVPAYNINFDNMAYYALGAYEVGPMPIIDIVAAAQQHV
CCCCCCEECCEEECCCCCCCEEEEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHHHH
DQAISLTLFMTDQVTTRDLNKAYIHAFKKKCASIYYVRVRQEVLEDSENYECDACVI
HHHHEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEC
>Mature Secondary Structure
MEDKKINTLSGTDESDEYVTLNARAKILAAGSDNFKLDVDAAKAYLKHHVEPRLVKFGSV
CCCCCCCCCCCCCCCCCEEEEECCEEEEEECCCCEEEEHHHHHHHHHHCCCCHHHHHCCH
KERIEYLVSNGYYEEELINKYTIEQIEEITDIAYGFHREFPSFMGAMKFYNSYGLKTFDG
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
QKYLENFEDRAVMNALFLGNGNFESATKTLRQLMLGRFQPATPTFLNAGKKQRGEYVSCY
HHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHCCCCCCCCHHHCCCCHHCCCEEEEE
LLRVEDNMESICRAVTTSLQLSKRGGGVALCLTNLRELGAPIKQIANQATGVIPVMKILE
EEEECCCHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHCCHHHHHHHHHCCHHHHHHHHH
DSFSYANQLGQRQGAGAVYLNAHHPDVMSFLDTKRENADEKIRIKSLSLGLVIPDITFEL
HHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCCCCCEEEEEEEEEEEEECCCHHHH
AQKNEEMALFSPYDVEREYGKAMSDISITQEYQNLVKNPNIKKTYINARKFFLTVAELHF
HCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHH
ESGYPYILFDDTVNNNNAHAATGRIVMSNLCSEIAQVSTPSTFNDDLSFKSMGDDICCNL
CCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCHHHCCC
GSLNIAKVMESGAEFAEVIEHSINALDLVSRTSDLSSAPSIQQGNKKNHAVGLGAMNLHG
CCCHHHHHHHCCHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECHHHHH
FLATNFIYNSIEAVDFTNMFFYAMAYNAFKASHKLALQYGTFDGFETSQFADGSFNKYTK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCHHHHH
CAEDKTPETQRVRELFAQYNVTIPTQKDIELVELIKKDGIANSHLMAVAPTGSISYLSSC
HCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHC
TPSLQPVVAPIEVRKEGKLGRIYVPAYNINFDNMAYYALGAYEVGPMPIIDIVAAAQQHV
CCCCCCEECCEEECCCCCCCEEEEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHHHH
DQAISLTLFMTDQVTTRDLNKAYIHAFKKKCASIYYVRVRQEVLEDSENYECDACVI
HHHHEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8948633 [H]