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The map label for this gene is amiE [H]

Identifier: 50364911

GI number: 50364911

Start: 117070

End: 118638

Strand: Direct

Name: amiE [H]

Synonym: Mfl096

Alternate gene names: 50364911

Gene position: 117070-118638 (Clockwise)

Preceding gene: 50364910

Following gene: 50364912

Centisome position: 14.76

GC content: 29.64

Gene sequence:

>1569_bases
ATGGCTACAAATAAAGATAAAATTATTTCTCTTCAAGATGTTGTTGTTAAATTCAATGTTCGTGGAAGAATGTTAACAGC
TATTAGAAACGTTTCTTTTGATATTTATGATGGAGAAACTGTTGCAATAGTTGGAGAATCTGGATCAGGAAAATCTGTAT
TAACTAAAACATTAACTAATATGTTAGAAAGTAATGGTTATATTTCAAACGGAACAATTATGTACTTTCCAACTGAAGAA
TCAAAAAACAATTCTGAAACAGCTGTTAAAGAAGATGTAAATTTAGTTAACTATCATAAAGGTTCATTAACTTCAGATAC
AAGAAGAAAAATTAAAAAAAGTAATTACAAAACAATTAATAATGCAAAAATTCGTTTAGAGGCACTTAACGCTCGTTTGG
GAGTTAAAGGTGTTGATACAGACGACATATTAGCAAAAATAAAACAAGAAAGTGAAATTATTCATGATGCATATTATGAA
CTTTCAACATTCTCAAGATTAAGAAAAAGAACAGTTTTACGTTTAACTCCAACAATGAAAGAAATTTCAAGTAAAAAACG
AAATCTTTCTCTAATAAAAGGAAGACAAATTCTTGCTGGTCTAAGAATTCTTGCAGAACAAGAGTTTTTAACAGAATTTG
AATTAAACTTGAAACATGTTTTAGAAAAATTAAAAAATGGTGAAACAGTTGAAGAACATGAAGTTAATACTTTATTGGCT
GCATGAAAATTTCAAACAACTCCAGCTTGATTAAACAAGAGAGAAGCAATAAAAAAATTAAAACAAGTTAGAGGGGGAAC
AATTGCTACAATTTTTCAAGACCCAATGACATCATTAAACCCATTACTAAGTGTAGGTTTTCAAATTTCAGAAGCAATCA
GATTGCATAATAAAGTTTCAAGACATGCTGCTAAACAACAAGCAATTGAATTAATGAACAAAGTAGGTATACCAAATGCT
GAAAAACGTTATCATGACATTCCAGGGAAATATTCAGGTGGTATGAGACAACGTATTGTTATTGCTATTGCATTAGCATG
TAAACCAAAAGTTTTAATTTGTGATGAACCAACAACAGCGCTTGATGTTACTATTCAAGCTCAAATTTTAGAGTTAATTA
AAGAACTTAAAAAAGAATTTAATTTAACTGTAATCTTTATTACTCATGACTTAGGTGTAGTTGCAAATGTTGCAGATCGT
GTTGCAGTATTATATGCTGGGCAAATTATTGAATATGGAACTGTTAAAGATATTTTCTTTGATGCAAGACACCCATATAC
TTGAGCATTGTTATCTTCATTACCTCAATTAGGAACAAAAGGTGAAGAATTATTTGCTATTACAGGTACACCTCCAAGTT
TATTTAACAAAATTAAAGGTGATGCATTTGCTCCAAGAAACAAATTTGCTTTAGCAATTGACTTTGAATATAATCCACCA
ATGTTTGAAATTAGTAAAACTCATGGTGCAAGAACTTGATTATTAGACCCAAGAGCACCTAAAGTTGAAAAACCTAAAAT
GTTAGAAAACTTAAAAGAAGCTGTAAGTGAAGCAAAGGTAGGTGAATAG

Upstream 100 bases:

>100_bases
CAATGGCAGTTATTATGATAGTTTCAGTTGTATTCTTCTTAGCTGCTAGAGTATTTGCTGACTCATTAGATCCGAAAAAT
CATAGATAGGAGACCAACTT

Downstream 100 bases:

>100_bases
TATGGCTAAGGAATCAATCATAAAAGTACGTGACCTTTTAATCGAATATGGGCACGGAAAAAATAAAGTTTCTGCTGTTA
AAGAAGTTTCTTTTGATATT

Product: oligopeptide ABC transporter ATP-binding component

Products: ADP; phosphate; oligopeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 522; Mature: 521

Protein sequence:

>522_residues
MATNKDKIISLQDVVVKFNVRGRMLTAIRNVSFDIYDGETVAIVGESGSGKSVLTKTLTNMLESNGYISNGTIMYFPTEE
SKNNSETAVKEDVNLVNYHKGSLTSDTRRKIKKSNYKTINNAKIRLEALNARLGVKGVDTDDILAKIKQESEIIHDAYYE
LSTFSRLRKRTVLRLTPTMKEISSKKRNLSLIKGRQILAGLRILAEQEFLTEFELNLKHVLEKLKNGETVEEHEVNTLLA
AWKFQTTPAWLNKREAIKKLKQVRGGTIATIFQDPMTSLNPLLSVGFQISEAIRLHNKVSRHAAKQQAIELMNKVGIPNA
EKRYHDIPGKYSGGMRQRIVIAIALACKPKVLICDEPTTALDVTIQAQILELIKELKKEFNLTVIFITHDLGVVANVADR
VAVLYAGQIIEYGTVKDIFFDARHPYTWALLSSLPQLGTKGEELFAITGTPPSLFNKIKGDAFAPRNKFALAIDFEYNPP
MFEISKTHGARTWLLDPRAPKVEKPKMLENLKEAVSEAKVGE

Sequences:

>Translated_522_residues
MATNKDKIISLQDVVVKFNVRGRMLTAIRNVSFDIYDGETVAIVGESGSGKSVLTKTLTNMLESNGYISNGTIMYFPTEE
SKNNSETAVKEDVNLVNYHKGSLTSDTRRKIKKSNYKTINNAKIRLEALNARLGVKGVDTDDILAKIKQESEIIHDAYYE
LSTFSRLRKRTVLRLTPTMKEISSKKRNLSLIKGRQILAGLRILAEQEFLTEFELNLKHVLEKLKNGETVEEHEVNTLLA
A*KFQTTPA*LNKREAIKKLKQVRGGTIATIFQDPMTSLNPLLSVGFQISEAIRLHNKVSRHAAKQQAIELMNKVGIPNA
EKRYHDIPGKYSGGMRQRIVIAIALACKPKVLICDEPTTALDVTIQAQILELIKELKKEFNLTVIFITHDLGVVANVADR
VAVLYAGQIIEYGTVKDIFFDARHPYT*ALLSSLPQLGTKGEELFAITGTPPSLFNKIKGDAFAPRNKFALAIDFEYNPP
MFEISKTHGART*LLDPRAPKVEKPKMLENLKEAVSEAKVGE
>Mature_521_residues
ATNKDKIISLQDVVVKFNVRGRMLTAIRNVSFDIYDGETVAIVGESGSGKSVLTKTLTNMLESNGYISNGTIMYFPTEES
KNNSETAVKEDVNLVNYHKGSLTSDTRRKIKKSNYKTINNAKIRLEALNARLGVKGVDTDDILAKIKQESEIIHDAYYEL
STFSRLRKRTVLRLTPTMKEISSKKRNLSLIKGRQILAGLRILAEQEFLTEFELNLKHVLEKLKNGETVEEHEVNTLLAA
*KFQTTPA*LNKREAIKKLKQVRGGTIATIFQDPMTSLNPLLSVGFQISEAIRLHNKVSRHAAKQQAIELMNKVGIPNAE
KRYHDIPGKYSGGMRQRIVIAIALACKPKVLICDEPTTALDVTIQAQILELIKELKKEFNLTVIFITHDLGVVANVADRV
AVLYAGQIIEYGTVKDIFFDARHPYT*ALLSSLPQLGTKGEELFAITGTPPSLFNKIKGDAFAPRNKFALAIDFEYNPPM
FEISKTHGART*LLDPRAPKVEKPKMLENLKEAVSEAKVGE

Specific function: Part of the binding-protein-dependent transport system for oligopeptides. Probably responsible for energy coupling to the transport system [H]

COG id: COG0444

COG function: function code EP; ABC-type dipeptide/oligopeptide/nickel transport system, ATPase component

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Escherichia coli, GI48994883, Length=252, Percent_Identity=46.031746031746, Blast_Score=234, Evalue=1e-62,
Organism=Escherichia coli, GI145693107, Length=181, Percent_Identity=58.0110497237569, Blast_Score=221, Evalue=6e-59,
Organism=Escherichia coli, GI1789963, Length=218, Percent_Identity=48.1651376146789, Blast_Score=221, Evalue=1e-58,
Organism=Escherichia coli, GI1787759, Length=222, Percent_Identity=45.4954954954955, Blast_Score=199, Evalue=3e-52,
Organism=Escherichia coli, GI1788506, Length=178, Percent_Identity=47.752808988764, Blast_Score=173, Evalue=3e-44,
Organism=Escherichia coli, GI1787500, Length=235, Percent_Identity=41.2765957446808, Blast_Score=167, Evalue=2e-42,
Organism=Escherichia coli, GI1789962, Length=209, Percent_Identity=40.6698564593301, Blast_Score=159, Evalue=4e-40,
Organism=Escherichia coli, GI1787758, Length=211, Percent_Identity=39.3364928909953, Blast_Score=146, Evalue=3e-36,
Organism=Escherichia coli, GI1787548, Length=225, Percent_Identity=33.3333333333333, Blast_Score=131, Evalue=9e-32,
Organism=Escherichia coli, GI48994997, Length=166, Percent_Identity=40.3614457831325, Blast_Score=123, Evalue=3e-29,
Organism=Escherichia coli, GI1789890, Length=181, Percent_Identity=39.7790055248619, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1786398, Length=125, Percent_Identity=43.2, Blast_Score=105, Evalue=8e-24,
Organism=Escherichia coli, GI1787547, Length=182, Percent_Identity=31.8681318681319, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1787712, Length=186, Percent_Identity=34.4086021505376, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1788225, Length=176, Percent_Identity=33.5227272727273, Blast_Score=96, Evalue=7e-21,
Organism=Escherichia coli, GI1789891, Length=152, Percent_Identity=34.8684210526316, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI1789032, Length=219, Percent_Identity=28.7671232876712, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI1790162, Length=154, Percent_Identity=33.7662337662338, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI87081709, Length=166, Percent_Identity=31.9277108433735, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI1788644, Length=128, Percent_Identity=35.9375, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1787029, Length=114, Percent_Identity=38.5964912280702, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1786872, Length=124, Percent_Identity=35.4838709677419, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1789672, Length=165, Percent_Identity=31.5151515151515, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1787089, Length=169, Percent_Identity=35.5029585798817, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1788761, Length=107, Percent_Identity=36.4485981308411, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1786345, Length=169, Percent_Identity=30.7692307692308, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI1787370, Length=161, Percent_Identity=27.9503105590062, Blast_Score=76, Evalue=6e-15,
Organism=Escherichia coli, GI1787164, Length=89, Percent_Identity=38.2022471910112, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1786563, Length=100, Percent_Identity=40, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786803, Length=87, Percent_Identity=40.2298850574713, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1786703, Length=153, Percent_Identity=33.3333333333333, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI1790190, Length=113, Percent_Identity=36.283185840708, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI1788450, Length=137, Percent_Identity=30.6569343065693, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1790534, Length=107, Percent_Identity=35.5140186915888, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI1790467, Length=92, Percent_Identity=34.7826086956522, Blast_Score=66, Evalue=6e-12,
Organism=Escherichia coli, GI87081791, Length=100, Percent_Identity=32, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1788165, Length=75, Percent_Identity=40, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1786253, Length=135, Percent_Identity=28.8888888888889, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1790739, Length=90, Percent_Identity=34.4444444444444, Blast_Score=64, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17565938, Length=113, Percent_Identity=40.7079646017699, Blast_Score=68, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593
- InterPro:   IPR010066
- InterPro:   IPR013563 [H]

Pfam domain/function: PF00005 ABC_tran; PF08352 oligo_HPY [H]

EC number: NA

Molecular weight: Translated: 57734; Mature: 57603

Theoretical pI: Translated: 10.02; Mature: 10.02

Prosite motif: PS00211 ABC_TRANSPORTER_1 ; PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATNKDKIISLQDVVVKFNVRGRMLTAIRNVSFDIYDGETVAIVGESGSGKSVLTKTLTN
CCCCCCCEEEEEEEEEEEECCCHHHHHHHHCEEEEECCCEEEEEECCCCCHHHHHHHHHH
MLESNGYISNGTIMYFPTEESKNNSETAVKEDVNLVNYHKGSLTSDTRRKIKKSNYKTIN
HHHCCCEEECCEEEEEECCCCCCCCCHHHHHCCCEEEECCCCCCHHHHHHHHHCCCCEEC
NAKIRLEALNARLGVKGVDTDDILAKIKQESEIIHDAYYELSTFSRLRKRTVLRLTPTMK
CCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCHH
EISSKKRNLSLIKGRQILAGLRILAEQEFLTEFELNLKHVLEKLKNGETVEEHEVNTLLA
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHEE
AKFQTTPALNKREAIKKLKQVRGGTIATIFQDPMTSLNPLLSVGFQISEAIRLHNKVSRH
EHCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAKQQAIELMNKVGIPNAEKRYHDIPGKYSGGMRQRIVIAIALACKPKVLICDEPTTALD
HHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCHHEEEEEEEEECCCEEEEECCCCCEEE
VTIQAQILELIKELKKEFNLTVIFITHDLGVVANVADRVAVLYAGQIIEYGTVKDIFFDA
EEHHHHHHHHHHHHHHHCCEEEEEEECCCHHHHHHHHHHHHHHHCCHHCCCCHHHHHCCC
RHPYTALLSSLPQLGTKGEELFAITGTPPSLFNKIKGDAFAPRNKFALAIDFEYNPPMFE
CCCHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHCCCCCCCCCCEEEEEEECCCCCCEE
ISKTHGARTLLDPRAPKVEKPKMLENLKEAVSEAKVGE
EECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
ATNKDKIISLQDVVVKFNVRGRMLTAIRNVSFDIYDGETVAIVGESGSGKSVLTKTLTN
CCCCCCEEEEEEEEEEEECCCHHHHHHHHCEEEEECCCEEEEEECCCCCHHHHHHHHHH
MLESNGYISNGTIMYFPTEESKNNSETAVKEDVNLVNYHKGSLTSDTRRKIKKSNYKTIN
HHHCCCEEECCEEEEEECCCCCCCCCHHHHHCCCEEEECCCCCCHHHHHHHHHCCCCEEC
NAKIRLEALNARLGVKGVDTDDILAKIKQESEIIHDAYYELSTFSRLRKRTVLRLTPTMK
CCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCHH
EISSKKRNLSLIKGRQILAGLRILAEQEFLTEFELNLKHVLEKLKNGETVEEHEVNTLLA
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHEE
AKFQTTPALNKREAIKKLKQVRGGTIATIFQDPMTSLNPLLSVGFQISEAIRLHNKVSRH
EHCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAKQQAIELMNKVGIPNAEKRYHDIPGKYSGGMRQRIVIAIALACKPKVLICDEPTTALD
HHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCHHEEEEEEEEECCCEEEEECCCCCEEE
VTIQAQILELIKELKKEFNLTVIFITHDLGVVANVADRVAVLYAGQIIEYGTVKDIFFDA
EEHHHHHHHHHHHHHHHCCEEEEEEECCCHHHHHHHHHHHHHHHCCHHCCCCHHHHHCCC
RHPYTALLSSLPQLGTKGEELFAITGTPPSLFNKIKGDAFAPRNKFALAIDFEYNPPMFE
CCCHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHCCCCCCCCCCEEEEEEECCCCCCEE
ISKTHGARTLLDPRAPKVEKPKMLENLKEAVSEAKVGE
EECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; oligopeptides [Periplasm]; H2O [C]

Specific reaction: ATP + oligopeptides [Periplasm] + H2O = ADP + phosphate + oligopeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2352474; 11463916 [H]