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The map label for this gene is cysS [H]

Identifier: 50364902

GI number: 50364902

Start: 108974

End: 110299

Strand: Direct

Name: cysS [H]

Synonym: Mfl087

Alternate gene names: 50364902

Gene position: 108974-110299 (Clockwise)

Preceding gene: 50364901

Following gene: 50364903

Centisome position: 13.74

GC content: 22.1

Gene sequence:

>1326_bases
ATGAAATTATTTGATACATTAACTCAAGAACAAAAAAACATAAATAAAGAAGTTATCAATATATATTCATGTGGTCCAAC
AATATATGACTATATTCATATAGGTAATGCTAGACCAATAATTTTAATGGATACTTTAATTAGATTTTTAGAATCAGAAG
GTATCAAAGTAAATTTTTTACAAAACATTACAGATATTGATGACAAAATTATTGAAAAAGCTATACAAGAAAATAAAACA
GAAAAAGAAATAACAGACAAATACTTGTCTGCATTTTTACAAAACTTAAAAGATTTGAAAATTAGAATGCCTGATAAATT
AATTCCCATAAGTGAAAAAATAAGTGAAATGAATTTATTTATTGACGATTTAGTTAAATTAGAAGCTGCATATAATGTTG
ATGGTGATGTTTATTTTGATATACAAAAATTTTCAGATGAATATGGGAAATTATCAAACAAAAAAATAAATGATTTAATT
TCAGGAAATAGAGTCGAAATAGAAGATAAAAAAAATAACCCATTAGATTTTAGCGTGTGAAAAAAAACTGAAAAAGGTAT
AACATTTGATTCTAATTTTGGTAAAGGTAGACCAGGTTGACATACAGAGTGTGCTTTATTGATTGATGAATATTTTAAAG
GTGAAACAATAGACATTCATTCAGGTGGTATTGACTTACAGTTTCCTCATCATGAAAATGAAAGAATTCAATTTATAGCA
AAACATGGAAAAGAAATATCTGATATTTGAGTTCACAATGGACATTTAACTATTAATGGCGAGAAAATGAGCAAATCATT
AGGAAATACAATGACACTTACTAATTTTTTAGCAAATTATAATTCAGATATATTAAGATGAATATTTTTAACAACTTACT
ACAAACAACCTTTAAATATAAACGATGATTTAATTGAACAAGCAAATAAATTTATTCAAAAAATAAATAATTTAAGCAAA
AAAATAAAACAATTATTAATTGAAAATAAATTTGTTAAAACTAACCAATTTGATGAAGAAATTATAAAACAATTTAAAAT
TCATATGAAAAATGACTTAAACACTTCAAGAGTTTTAACTTTATTAGAAGATACACTTAAAGATATTAATAAGCTTTCAA
CATTAAAAGAATTTGATGATTTATTTTTAAAAATAAATTCTTTAAATTACATTTTAAAAACATTAGGTCTTTCAATAAAC
ATAAATACTTTTGTTTCTGATGAAGAACAAAATTTATTTTTATTATGAAAAAAAATAGTAAGTGAAAAAGATTTTGAAAA
AGCAGACGAAATAAGAAAAGTATTAATTAGTAAAGGGATTTTATAA

Upstream 100 bases:

>100_bases
AGTTCAAATCCCATTAAAAATTATTTTTAATACAGCTTTACTAGCAACTGTTTATTCAGTCTTAAGACCATTAATTAAAA
AATAATAAGAAAGGATCTAT

Downstream 100 bases:

>100_bases
AATGGAGAATTTAAGTTTAATTTACGGAAAACATGCAGTTAATGAATTTATAAAAAAGCACCCTAATTTAATTAAAAAAA
TATATATTTCAAGTGACTTT

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS [H]

Number of amino acids: Translated: 441; Mature: 441

Protein sequence:

>441_residues
MKLFDTLTQEQKNINKEVINIYSCGPTIYDYIHIGNARPIILMDTLIRFLESEGIKVNFLQNITDIDDKIIEKAIQENKT
EKEITDKYLSAFLQNLKDLKIRMPDKLIPISEKISEMNLFIDDLVKLEAAYNVDGDVYFDIQKFSDEYGKLSNKKINDLI
SGNRVEIEDKKNNPLDFSVWKKTEKGITFDSNFGKGRPGWHTECALLIDEYFKGETIDIHSGGIDLQFPHHENERIQFIA
KHGKEISDIWVHNGHLTINGEKMSKSLGNTMTLTNFLANYNSDILRWIFLTTYYKQPLNINDDLIEQANKFIQKINNLSK
KIKQLLIENKFVKTNQFDEEIIKQFKIHMKNDLNTSRVLTLLEDTLKDINKLSTLKEFDDLFLKINSLNYILKTLGLSIN
INTFVSDEEQNLFLLWKKIVSEKDFEKADEIRKVLISKGIL

Sequences:

>Translated_441_residues
MKLFDTLTQEQKNINKEVINIYSCGPTIYDYIHIGNARPIILMDTLIRFLESEGIKVNFLQNITDIDDKIIEKAIQENKT
EKEITDKYLSAFLQNLKDLKIRMPDKLIPISEKISEMNLFIDDLVKLEAAYNVDGDVYFDIQKFSDEYGKLSNKKINDLI
SGNRVEIEDKKNNPLDFSV*KKTEKGITFDSNFGKGRPG*HTECALLIDEYFKGETIDIHSGGIDLQFPHHENERIQFIA
KHGKEISDI*VHNGHLTINGEKMSKSLGNTMTLTNFLANYNSDILR*IFLTTYYKQPLNINDDLIEQANKFIQKINNLSK
KIKQLLIENKFVKTNQFDEEIIKQFKIHMKNDLNTSRVLTLLEDTLKDINKLSTLKEFDDLFLKINSLNYILKTLGLSIN
INTFVSDEEQNLFLL*KKIVSEKDFEKADEIRKVLISKGIL
>Mature_441_residues
MKLFDTLTQEQKNINKEVINIYSCGPTIYDYIHIGNARPIILMDTLIRFLESEGIKVNFLQNITDIDDKIIEKAIQENKT
EKEITDKYLSAFLQNLKDLKIRMPDKLIPISEKISEMNLFIDDLVKLEAAYNVDGDVYFDIQKFSDEYGKLSNKKINDLI
SGNRVEIEDKKNNPLDFSV*KKTEKGITFDSNFGKGRPG*HTECALLIDEYFKGETIDIHSGGIDLQFPHHENERIQFIA
KHGKEISDI*VHNGHLTINGEKMSKSLGNTMTLTNFLANYNSDILR*IFLTTYYKQPLNINDDLIEQANKFIQKINNLSK
KIKQLLIENKFVKTNQFDEEIIKQFKIHMKNDLNTSRVLTLLEDTLKDINKLSTLKEFDDLFLKINSLNYILKTLGLSIN
INTFVSDEEQNLFLL*KKIVSEKDFEKADEIRKVLISKGIL

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI13375695, Length=443, Percent_Identity=29.1196388261851, Blast_Score=186, Evalue=5e-47,
Organism=Homo sapiens, GI303304987, Length=305, Percent_Identity=30.4918032786885, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI21269877, Length=305, Percent_Identity=30.4918032786885, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI10835051, Length=305, Percent_Identity=30.4918032786885, Blast_Score=130, Evalue=3e-30,
Organism=Homo sapiens, GI62240992, Length=305, Percent_Identity=30.4918032786885, Blast_Score=130, Evalue=3e-30,
Organism=Escherichia coli, GI1786737, Length=377, Percent_Identity=32.8912466843501, Blast_Score=211, Evalue=7e-56,
Organism=Caenorhabditis elegans, GI17509795, Length=268, Percent_Identity=30.9701492537313, Blast_Score=108, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17509797, Length=268, Percent_Identity=30.9701492537313, Blast_Score=108, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6324082, Length=387, Percent_Identity=29.1989664082687, Blast_Score=124, Evalue=2e-29,
Organism=Drosophila melanogaster, GI19922218, Length=416, Percent_Identity=32.2115384615385, Blast_Score=191, Evalue=7e-49,
Organism=Drosophila melanogaster, GI19922364, Length=277, Percent_Identity=33.2129963898917, Blast_Score=134, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015803
- InterPro:   IPR015273
- InterPro:   IPR014729
- InterPro:   IPR009080 [H]

Pfam domain/function: PF01406 tRNA-synt_1e [H]

EC number: =6.1.1.16 [H]

Molecular weight: Translated: 50326; Mature: 50326

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLFDTLTQEQKNINKEVINIYSCGPTIYDYIHIGNARPIILMDTLIRFLESEGIKVNFL
CCHHHHHHHHHHCCCHHHEEEEECCCHHHHHEEECCCCCCHHHHHHHHHHHCCCCEEEEH
QNITDIDDKIIEKAIQENKTEKEITDKYLSAFLQNLKDLKIRMPDKLIPISEKISEMNLF
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCEEECCCCCCCHHHHHHHHHHH
IDDLVKLEAAYNVDGDVYFDIQKFSDEYGKLSNKKINDLISGNRVEIEDKKNNPLDFSVK
HHHHHHHHHHCCCCCCEEEEHHHHHHHHCCCCCCHHHHHHCCCEEEEECCCCCCCCEEEE
KTEKGITFDSNFGKGRPGHTECALLIDEYFKGETIDIHSGGIDLQFPHHENERIQFIAKH
CCCCCCEECCCCCCCCCCCCHHHHHHHHHHCCCEEEEECCCEEEECCCCCCHHHHHHHHC
GKEISDIVHNGHLTINGEKMSKSLGNTMTLTNFLANYNSDILRIFLTTYYKQPLNINDDL
CCHHHHHHHCCCEEEEHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHH
IEQANKFIQKINNLSKKIKQLLIENKFVKTNQFDEEIIKQFKIHMKNDLNTSRVLTLLED
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHH
TLKDINKLSTLKEFDDLFLKINSLNYILKTLGLSININTFVSDEEQNLFLLKKIVSEKDF
HHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHCCEEEEEEEECCCCCCHHHHHHHHCCCCH
EKADEIRKVLISKGIL
HHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKLFDTLTQEQKNINKEVINIYSCGPTIYDYIHIGNARPIILMDTLIRFLESEGIKVNFL
CCHHHHHHHHHHCCCHHHEEEEECCCHHHHHEEECCCCCCHHHHHHHHHHHCCCCEEEEH
QNITDIDDKIIEKAIQENKTEKEITDKYLSAFLQNLKDLKIRMPDKLIPISEKISEMNLF
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCEEECCCCCCCHHHHHHHHHHH
IDDLVKLEAAYNVDGDVYFDIQKFSDEYGKLSNKKINDLISGNRVEIEDKKNNPLDFSVK
HHHHHHHHHHCCCCCCEEEEHHHHHHHHCCCCCCHHHHHHCCCEEEEECCCCCCCCEEEE
KTEKGITFDSNFGKGRPGHTECALLIDEYFKGETIDIHSGGIDLQFPHHENERIQFIAKH
CCCCCCEECCCCCCCCCCCCHHHHHHHHHHCCCEEEEECCCEEEECCCCCCHHHHHHHHC
GKEISDIVHNGHLTINGEKMSKSLGNTMTLTNFLANYNSDILRIFLTTYYKQPLNINDDL
CCHHHHHHHCCCEEEEHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHH
IEQANKFIQKINNLSKKIKQLLIENKFVKTNQFDEEIIKQFKIHMKNDLNTSRVLTLLED
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHH
TLKDINKLSTLKEFDDLFLKINSLNYILKTLGLSININTFVSDEEQNLFLLKKIVSEKDF
HHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHCCEEEEEEEECCCCCCHHHHHHHHCCCCH
EKADEIRKVLISKGIL
HHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA