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The map label for this gene is pth [H]

Identifier: 50364892

GI number: 50364892

Start: 99130

End: 99690

Strand: Direct

Name: pth [H]

Synonym: Mfl077

Alternate gene names: 50364892

Gene position: 99130-99690 (Clockwise)

Preceding gene: 50364891

Following gene: 50364894

Centisome position: 12.5

GC content: 27.63

Gene sequence:

>561_bases
ATGAAATTAATAGTAGGATTAGGAAATTATGGTTCTCAATATGAAGCTACAAGACATAATGCTGGTTGAATAGCATTAGA
TCAGCTTATTGAAAAATATGGTTTTACGCAACAAAAAAATGAGCATAACTCAATAATTTTCTTTTCAACTGTTAATAATG
AAAAAGTATTATTCGTAAAACCCCAAACTTATATGAACAATTCAGGAATTGCAATTCAAGCAATCATGCATTACTATAAA
ATTGAAATTAAAGATTTAGTTATTTTGCATGATGAAAAAGATTTTCCTGTTGGGAAAAATCAATTTAAAATGAATGGTTC
TGCAGCAGGCCATAATGGAATAAAATCAGTAATTCAATATTTGGGAACTCAAAATTTTAATAGATACAGAATTGGAATTG
GTCAACCTGAACAGGGCTGAAAAATAATAGACTGAGTTTTATCAAAATTCAAACCTGAGGAATTGGAAAACATTATTTTA
GCCTCAAAAAGTATTTCAAACTTTGTCAATGATTGAACAAAAGGTACAACTTTTCAAAACATAATGAATTTATACAACTA
A

Upstream 100 bases:

>100_bases
GAATCTTTAACTGAAGTTTATAATAATAAAAAACAAATAATTTGAGATAGAGCTCAATGTATTGTTAATAAATTTAAAAA
ATAATTAAGAGGAATATAAA

Downstream 100 bases:

>100_bases
AAGTTGTATTTTTTTATTTTTCAAAAACAAAAGTAAGAGTTTTTAAATTCAAGTTTTTAATGTTGTTGTAATTTTTAATG
GTTTTTATGTTTTTAAATTC

Product: peptidyl-tRNA hydrolase

Products: NA

Alternate protein names: PTH [H]

Number of amino acids: Translated: 186; Mature: 186

Protein sequence:

>186_residues
MKLIVGLGNYGSQYEATRHNAGWIALDQLIEKYGFTQQKNEHNSIIFFSTVNNEKVLFVKPQTYMNNSGIAIQAIMHYYK
IEIKDLVILHDEKDFPVGKNQFKMNGSAAGHNGIKSVIQYLGTQNFNRYRIGIGQPEQGWKIIDWVLSKFKPEELENIIL
ASKSISNFVNDWTKGTTFQNIMNLYN

Sequences:

>Translated_186_residues
MKLIVGLGNYGSQYEATRHNAG*IALDQLIEKYGFTQQKNEHNSIIFFSTVNNEKVLFVKPQTYMNNSGIAIQAIMHYYK
IEIKDLVILHDEKDFPVGKNQFKMNGSAAGHNGIKSVIQYLGTQNFNRYRIGIGQPEQG*KIID*VLSKFKPEELENIIL
ASKSISNFVND*TKGTTFQNIMNLYN
>Mature_186_residues
MKLIVGLGNYGSQYEATRHNAG*IALDQLIEKYGFTQQKNEHNSIIFFSTVNNEKVLFVKPQTYMNNSGIAIQAIMHYYK
IEIKDLVILHDEKDFPVGKNQFKMNGSAAGHNGIKSVIQYLGTQNFNRYRIGIGQPEQG*KIID*VLSKFKPEELENIIL
ASKSISNFVND*TKGTTFQNIMNLYN

Specific function: The natural substrate for this enzyme may be peptidyl- tRNAs which drop off the ribosome during protein synthesis [H]

COG id: COG0193

COG function: function code J; Peptidyl-tRNA hydrolase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PTH family [H]

Homologues:

Organism=Homo sapiens, GI50897284, Length=172, Percent_Identity=29.0697674418605, Blast_Score=100, Evalue=7e-22,
Organism=Escherichia coli, GI1787455, Length=138, Percent_Identity=41.304347826087, Blast_Score=96, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001328
- InterPro:   IPR018171 [H]

Pfam domain/function: PF01195 Pept_tRNA_hydro [H]

EC number: =3.1.1.29 [H]

Molecular weight: Translated: 20554; Mature: 20554

Theoretical pI: Translated: 9.41; Mature: 9.41

Prosite motif: PS01196 PEPT_TRNA_HYDROL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLIVGLGNYGSQYEATRHNAGIALDQLIEKYGFTQQKNEHNSIIFFSTVNNEKVLFVKP
CEEEEEECCCCCCCCCHHCCCCCHHHHHHHHHCCCCCCCCCCCEEEEEECCCCEEEEECC
QTYMNNSGIAIQAIMHYYKIEIKDLVILHDEKDFPVGKNQFKMNGSAAGHNGIKSVIQYL
HHHCCCCCHHHHHHHHHHHEEEEEEEEEECCCCCCCCCCEEEECCCCCCCHHHHHHHHHH
GTQNFNRYRIGIGQPEQGKIIDVLSKFKPEELENIILASKSISNFVNDTKGTTFQNIMNL
CCCCCCEEEEECCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
YN
CC
>Mature Secondary Structure
MKLIVGLGNYGSQYEATRHNAGIALDQLIEKYGFTQQKNEHNSIIFFSTVNNEKVLFVKP
CEEEEEECCCCCCCCCHHCCCCCHHHHHHHHHCCCCCCCCCCCEEEEEECCCCEEEEECC
QTYMNNSGIAIQAIMHYYKIEIKDLVILHDEKDFPVGKNQFKMNGSAAGHNGIKSVIQYL
HHHCCCCCHHHHHHHHHHHEEEEEEEEEECCCCCCCCCCEEEECCCCCCCHHHHHHHHHH
GTQNFNRYRIGIGQPEQGKIIDVLSKFKPEELENIILASKSISNFVNDTKGTTFQNIMNL
CCCCCCEEEEECCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
YN
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA