Click here to switch to the map view.

The map label for this gene is pdhA [H]

Identifier: 50364854

GI number: 50364854

Start: 53676

End: 54788

Strand: Direct

Name: pdhA [H]

Synonym: Mfl039

Alternate gene names: 50364854

Gene position: 53676-54788 (Clockwise)

Preceding gene: 50364853

Following gene: 50364855

Centisome position: 6.77

GC content: 32.7

Gene sequence:

>1113_bases
ATGAAATATATAGGAAAATTTGATCCTCAAAAAGATGAAATTGTTCGTGTAATGGATAAAGATGGTAAAATCATTAATCC
TAAATTAGCTCCATCAATTTCTGATGAAGAATTAATAAAAGCATACTCAATTATGAATCTTTCAAGAAGACAAGATGATT
ACCAAAACAAAATGCAAAGACAAGGAAGATTATTATCTTTCTTAAGTTCAACAGGACAAGAAGCTTGTGAAGTTGCATAT
ACAATGGTTATTGATCCAAAGAACGATTTCTTCGTTTCTGGATATAGAAATAACGCAGCTTGATTGACAATGGGTCAAAC
AGTTAGAAACATTATGTTATATTGAGCAGGAAATGAAGCTGGAGCAAAAGCACCAGAAGGTGTTAATTCATTACCACCAA
ACATTATTATTGGTTCACAATACTCTCAAGCAACTGGAATTGCTTTTGCTGAAAAATACCAAGGTAAAAAAGGTGTAGCA
GTTACAACAACTGGAGATGGTGGAATGAGTGAAGGTGAAACTTATGAAGCAATGAACTTCGCTAAGTTACATGAATTACC
AGTTGTATTTGTTTGTGAAAATAACAAATGAGCTATTTCAACTCCAACTGTTCAACAAACAAAATCTTTAAATATTGCAG
TTAAAGCAATAGCAACAGGAACTCCATCAATTAAAGTTGACGGAAATGACTTCTTAGCAAGTTATGCTGTAGCAAAAGAA
GCTGTTGAATTTGCAAGAAGCGGAAACGGTCCTGTATTAATTGAATTTGATACATATAGACTTGGAGCCCACTCTTCATC
AGATGCACCAGACGTTTACCGTCCAAAAGGTGAATTTGAAGATAGAGTTCCTTATGAACCATTAATAAGATTAAAAGCTT
ATATGATTGAAAAAGGAATTTGAAGTGAAGAAAAACAAACTGCTTTAAATGAAGAGCAAGATAAACATATTGCAGCTGAA
TTTGCTTGAGTAGAACAAAATAAAAACTACCCAATTGAAGATATATTCAACTATCAATATGCTGAATTAACAAATGATTT
AAAAGATCAACTAGCAGAAGCTAAAGAGTTTTTTGCTAAATACCCAGAAACTAAAGACGGAGGACACCACTAA

Upstream 100 bases:

>100_bases
AAAAACATTCTAGATAAGTTTGCATTAAAAGAAATATTTGGTGATAACTTTGAAGCAGAAGATATTTTGAATTTAATATT
CAATTAAGAAAGGTATAAAT

Downstream 100 bases:

>100_bases
TGGCAGTTATTAATAATATTAAAGCAGTTACTGAAGCACTTGATGTTGCAATGGACCGTGATAAAAACGTTATTGTTTTT
GGTGAGGACGTTGGTTTAGA

Product: pyruvate dehydrogenase E1 alpha subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MKYIGKFDPQKDEIVRVMDKDGKIINPKLAPSISDEELIKAYSIMNLSRRQDDYQNKMQRQGRLLSFLSSTGQEACEVAY
TMVIDPKNDFFVSGYRNNAAWLTMGQTVRNIMLYWAGNEAGAKAPEGVNSLPPNIIIGSQYSQATGIAFAEKYQGKKGVA
VTTTGDGGMSEGETYEAMNFAKLHELPVVFVCENNKWAISTPTVQQTKSLNIAVKAIATGTPSIKVDGNDFLASYAVAKE
AVEFARSGNGPVLIEFDTYRLGAHSSSDAPDVYRPKGEFEDRVPYEPLIRLKAYMIEKGIWSEEKQTALNEEQDKHIAAE
FAWVEQNKNYPIEDIFNYQYAELTNDLKDQLAEAKEFFAKYPETKDGGHH

Sequences:

>Translated_370_residues
MKYIGKFDPQKDEIVRVMDKDGKIINPKLAPSISDEELIKAYSIMNLSRRQDDYQNKMQRQGRLLSFLSSTGQEACEVAY
TMVIDPKNDFFVSGYRNNAA*LTMGQTVRNIMLY*AGNEAGAKAPEGVNSLPPNIIIGSQYSQATGIAFAEKYQGKKGVA
VTTTGDGGMSEGETYEAMNFAKLHELPVVFVCENNK*AISTPTVQQTKSLNIAVKAIATGTPSIKVDGNDFLASYAVAKE
AVEFARSGNGPVLIEFDTYRLGAHSSSDAPDVYRPKGEFEDRVPYEPLIRLKAYMIEKGI*SEEKQTALNEEQDKHIAAE
FA*VEQNKNYPIEDIFNYQYAELTNDLKDQLAEAKEFFAKYPETKDGGHH
>Mature_370_residues
MKYIGKFDPQKDEIVRVMDKDGKIINPKLAPSISDEELIKAYSIMNLSRRQDDYQNKMQRQGRLLSFLSSTGQEACEVAY
TMVIDPKNDFFVSGYRNNAA*LTMGQTVRNIMLY*AGNEAGAKAPEGVNSLPPNIIIGSQYSQATGIAFAEKYQGKKGVA
VTTTGDGGMSEGETYEAMNFAKLHELPVVFVCENNK*AISTPTVQQTKSLNIAVKAIATGTPSIKVDGNDFLASYAVAKE
AVEFARSGNGPVLIEFDTYRLGAHSSSDAPDVYRPKGEFEDRVPYEPLIRLKAYMIEKGI*SEEKQTALNEEQDKHIAAE
FA*VEQNKNYPIEDIFNYQYAELTNDLKDQLAEAKEFFAKYPETKDGGHH

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG1071

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI11386135, Length=363, Percent_Identity=33.3333333333333, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI258645172, Length=363, Percent_Identity=33.3333333333333, Blast_Score=159, Evalue=4e-39,
Organism=Homo sapiens, GI291084742, Length=335, Percent_Identity=30.4477611940298, Blast_Score=139, Evalue=4e-33,
Organism=Homo sapiens, GI4505685, Length=335, Percent_Identity=30.4477611940298, Blast_Score=139, Evalue=4e-33,
Organism=Homo sapiens, GI4885543, Length=307, Percent_Identity=32.5732899022801, Blast_Score=136, Evalue=3e-32,
Organism=Homo sapiens, GI291084744, Length=342, Percent_Identity=30.7017543859649, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI291084757, Length=316, Percent_Identity=27.5316455696203, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI86563355, Length=361, Percent_Identity=35.1800554016621, Blast_Score=169, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI86563357, Length=361, Percent_Identity=35.1800554016621, Blast_Score=169, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI17536047, Length=299, Percent_Identity=31.438127090301, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI32564172, Length=299, Percent_Identity=31.438127090301, Blast_Score=136, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6321026, Length=288, Percent_Identity=31.9444444444444, Blast_Score=137, Evalue=3e-33,
Organism=Drosophila melanogaster, GI21355903, Length=364, Percent_Identity=31.3186813186813, Blast_Score=142, Evalue=5e-34,
Organism=Drosophila melanogaster, GI24639740, Length=282, Percent_Identity=29.0780141843972, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24639744, Length=282, Percent_Identity=29.0780141843972, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI28571106, Length=282, Percent_Identity=29.0780141843972, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24639746, Length=237, Percent_Identity=31.6455696202532, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24639748, Length=299, Percent_Identity=27.4247491638796, Blast_Score=106, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001017
- InterPro:   IPR017596 [H]

Pfam domain/function: PF00676 E1_dh [H]

EC number: =1.2.4.1 [H]

Molecular weight: Translated: 40378; Mature: 40378

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYIGKFDPQKDEIVRVMDKDGKIINPKLAPSISDEELIKAYSIMNLSRRQDDYQNKMQR
CCCCCCCCCCHHHHHHEECCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
QGRLLSFLSSTGQEACEVAYTMVIDPKNDFFVSGYRNNAALTMGQTVRNIMLYAGNEAGA
HHHHHHHHHHCCHHHHEEEEEEEECCCCCEEEEECCCCEEEEHHHHHHEEEEEECCCCCC
KAPEGVNSLPPNIIIGSQYSQATGIAFAEKYQGKKGVAVTTTGDGGMSEGETYEAMNFAK
CCCCCCCCCCCCEEEECCCCCHHCCHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHH
LHELPVVFVCENNKAISTPTVQQTKSLNIAVKAIATGTPSIKVDGNDFLASYAVAKEAVE
HHCCCEEEEECCCCEECCCCHHHHCCCEEEEEEEECCCCEEEECCHHHHHHHHHHHHHHH
FARSGNGPVLIEFDTYRLGAHSSSDAPDVYRPKGEFEDRVPYEPLIRLKAYMIEKGISEE
HHHCCCCCEEEEECCEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHH
KQTALNEEQDKHIAAEFAVEQNKNYPIEDIFNYQYAELTNDLKDQLAEAKEFFAKYPETK
HHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
DGGHH
CCCCC
>Mature Secondary Structure
MKYIGKFDPQKDEIVRVMDKDGKIINPKLAPSISDEELIKAYSIMNLSRRQDDYQNKMQR
CCCCCCCCCCHHHHHHEECCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
QGRLLSFLSSTGQEACEVAYTMVIDPKNDFFVSGYRNNAALTMGQTVRNIMLYAGNEAGA
HHHHHHHHHHCCHHHHEEEEEEEECCCCCEEEEECCCCEEEEHHHHHHEEEEEECCCCCC
KAPEGVNSLPPNIIIGSQYSQATGIAFAEKYQGKKGVAVTTTGDGGMSEGETYEAMNFAK
CCCCCCCCCCCCEEEECCCCCHHCCHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHH
LHELPVVFVCENNKAISTPTVQQTKSLNIAVKAIATGTPSIKVDGNDFLASYAVAKEAVE
HHCCCEEEEECCCCEECCCCHHHHCCCEEEEEEEECCCCEEEECCHHHHHHHHHHHHHHH
FARSGNGPVLIEFDTYRLGAHSSSDAPDVYRPKGEFEDRVPYEPLIRLKAYMIEKGISEE
HHHCCCCCEEEEECCEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHH
KQTALNEEQDKHIAAEFAVEQNKNYPIEDIFNYQYAELTNDLKDQLAEAKEFFAKYPETK
HHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
DGGHH
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8948633 [H]