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The map label for this gene is dus1 [H]

Identifier: 50364844

GI number: 50364844

Start: 40465

End: 41442

Strand: Direct

Name: dus1 [H]

Synonym: Mfl029

Alternate gene names: 50364844

Gene position: 40465-41442 (Clockwise)

Preceding gene: 50364843

Following gene: 50364845

Centisome position: 5.1

GC content: 31.29

Gene sequence:

>978_bases
ATGAAAATAGGAAATGTAGAAATAAAAGGTAAATTAGTTCAAGGGCCAATGGCTGGTGTATCTAATCCACCATTTAGATT
AATATCAAAAGAAAAAGGTGCTGATTTAGTTTGTGCTGAAATGGTTTCTGTAGAAGGAATGACACATAACAACAAAAAAA
CATTTAGCATGTTAGCAGTTGAAAAAAAAGAACACCCAATGAGTATGCAAATTTTTGGGAATGATGTTGAATCATTTATT
GTGGCGACAAAGTGAATTGATGAAAATGTTGATTGTGACATTATAGATTTAAACATTGGTTGTCCTGCACCAAAAGTAGC
TGTTAGATCAGCGAGTGGATCAAGTTTATTAAAGACACCGGAATTAATATATGACATTGTAAAAGCAGTTGTTGAAAATA
CATCAAAGCCAGTTACTGCAAAAATTAGATTAGGTTGAGATAAAGAAAGTGTTAATGCTGTTGAAGTTTCTAAATTAATT
GAAAAAGCAGGAGCTAGCGGAATTACTGTTCATGGAAGAACAAGAAGTGAATTTTATTCAGGCCATGCAGATTGAGAAAA
AATAAAAGAAGTTAAAGAAGCTGTAAATATTCCAGTTATTGGTAATGGTGATGTTATTGACGGGCCAAGCGCAAAAGCAA
TGTTAGAATTAACTGGTTGTGATGGAGTAATGATTTCAAGAGGATGCCAAGGTAATCCTTGAATTTTTAGAGAAATTGCA
CACTTTTTAGAGACAGGTGAAGAATTAGATAAGCCAAGTTTTGCAGAATGAAAAGAAACAGTATTGAAGCATGCTAAAAT
GTTAATAGATTATAAACAAGACGAAGGTAGTGCAATTAGAGAATTTAGAAAACATTTAACTTGATATTTTGCAGCATTGC
CAAAAACAGAAATTTTAAATCAGTTAAAAGAAGAAGCAAATAAGATTAACACATTTAGTGATTTAGAAAATATTATAGAA
AAGTATAAAGAGGTATAA

Upstream 100 bases:

>100_bases
ACTTAACCAATTTTTTTGGTAACCAAGGGGTTAATGTATCTGTAAAATTTACTGAAATAAATAATGACTTATACGTTAAT
AACGAAAAAAGAGGATAAGT

Downstream 100 bases:

>100_bases
AATGTCAGAAAGAAAATTTACTGAACAAGAATTAGTTCGTAGAGCTAAACTTGAAAAATTAATTGAAAACAAATCTAATC
CTTATGAAGTTAGCAAGTAT

Product: tRNA dihydrouridine synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MKIGNVEIKGKLVQGPMAGVSNPPFRLISKEKGADLVCAEMVSVEGMTHNNKKTFSMLAVEKKEHPMSMQIFGNDVESFI
VATKWIDENVDCDIIDLNIGCPAPKVAVRSASGSSLLKTPELIYDIVKAVVENTSKPVTAKIRLGWDKESVNAVEVSKLI
EKAGASGITVHGRTRSEFYSGHADWEKIKEVKEAVNIPVIGNGDVIDGPSAKAMLELTGCDGVMISRGCQGNPWIFREIA
HFLETGEELDKPSFAEWKETVLKHAKMLIDYKQDEGSAIREFRKHLTWYFAALPKTEILNQLKEEANKINTFSDLENIIE
KYKEV

Sequences:

>Translated_325_residues
MKIGNVEIKGKLVQGPMAGVSNPPFRLISKEKGADLVCAEMVSVEGMTHNNKKTFSMLAVEKKEHPMSMQIFGNDVESFI
VATK*IDENVDCDIIDLNIGCPAPKVAVRSASGSSLLKTPELIYDIVKAVVENTSKPVTAKIRLG*DKESVNAVEVSKLI
EKAGASGITVHGRTRSEFYSGHAD*EKIKEVKEAVNIPVIGNGDVIDGPSAKAMLELTGCDGVMISRGCQGNP*IFREIA
HFLETGEELDKPSFAE*KETVLKHAKMLIDYKQDEGSAIREFRKHLT*YFAALPKTEILNQLKEEANKINTFSDLENIIE
KYKEV
>Mature_325_residues
MKIGNVEIKGKLVQGPMAGVSNPPFRLISKEKGADLVCAEMVSVEGMTHNNKKTFSMLAVEKKEHPMSMQIFGNDVESFI
VATK*IDENVDCDIIDLNIGCPAPKVAVRSASGSSLLKTPELIYDIVKAVVENTSKPVTAKIRLG*DKESVNAVEVSKLI
EKAGASGITVHGRTRSEFYSGHAD*EKIKEVKEAVNIPVIGNGDVIDGPSAKAMLELTGCDGVMISRGCQGNP*IFREIA
HFLETGEELDKPSFAE*KETVLKHAKMLIDYKQDEGSAIREFRKHLT*YFAALPKTEILNQLKEEANKINTFSDLENIIE
KYKEV

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=250, Percent_Identity=30.4, Blast_Score=118, Evalue=6e-27,
Organism=Homo sapiens, GI239788462, Length=251, Percent_Identity=30.2788844621514, Blast_Score=116, Evalue=4e-26,
Organism=Homo sapiens, GI31742496, Length=311, Percent_Identity=30.2250803858521, Blast_Score=115, Evalue=6e-26,
Organism=Homo sapiens, GI40807366, Length=311, Percent_Identity=29.903536977492, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI8923374, Length=245, Percent_Identity=29.7959183673469, Blast_Score=96, Evalue=4e-20,
Organism=Escherichia coli, GI1789660, Length=324, Percent_Identity=37.3456790123457, Blast_Score=197, Evalue=8e-52,
Organism=Escherichia coli, GI1788462, Length=151, Percent_Identity=39.0728476821192, Blast_Score=110, Evalue=9e-26,
Organism=Escherichia coli, GI145693211, Length=321, Percent_Identity=24.2990654205607, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17507177, Length=309, Percent_Identity=30.7443365695793, Blast_Score=117, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI17543114, Length=238, Percent_Identity=31.0924369747899, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI25144369, Length=219, Percent_Identity=31.0502283105023, Blast_Score=91, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17510279, Length=282, Percent_Identity=30.1418439716312, Blast_Score=79, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6323560, Length=226, Percent_Identity=30.9734513274336, Blast_Score=100, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6323437, Length=225, Percent_Identity=30.6666666666667, Blast_Score=98, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6323433, Length=313, Percent_Identity=26.1980830670927, Blast_Score=86, Evalue=7e-18,
Organism=Saccharomyces cerevisiae, GI6324342, Length=229, Percent_Identity=29.2576419213974, Blast_Score=71, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24585320, Length=240, Percent_Identity=34.5833333333333, Blast_Score=115, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24580595, Length=279, Percent_Identity=29.0322580645161, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI19920448, Length=279, Percent_Identity=29.0322580645161, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI19921524, Length=224, Percent_Identity=29.0178571428571, Blast_Score=95, Evalue=5e-20,
Organism=Drosophila melanogaster, GI45549423, Length=252, Percent_Identity=26.1904761904762, Blast_Score=78, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 34841; Mature: 34841

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIGNVEIKGKLVQGPMAGVSNPPFRLISKEKGADLVCAEMVSVEGMTHNNKKTFSMLAV
CCCCCEEEEEEEEECCCCCCCCCCCEEEECCCCCCHHHHHHHHHCCCCCCCCCEEEEEEE
EKKEHPMSMQIFGNDVESFIVATKIDENVDCDIIDLNIGCPAPKVAVRSASGSSLLKTPE
HHCCCCCEEEEECCCHHHEEEEEECCCCCCCEEEEEECCCCCCHHEEECCCCCCHHCCHH
LIYDIVKAVVENTSKPVTAKIRLGDKESVNAVEVSKLIEKAGASGITVHGRTRSEFYSGH
HHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEECCCHHHHHCCC
ADEKIKEVKEAVNIPVIGNGDVIDGPSAKAMLELTGCDGVMISRGCQGNPIFREIAHFLE
CHHHHHHHHHHHCCCEECCCCEECCCCCCEEEEEECCCCEEECCCCCCCHHHHHHHHHHH
TGEELDKPSFAEKETVLKHAKMLIDYKQDEGSAIREFRKHLTYFAALPKTEILNQLKEEA
CHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
NKINTFSDLENIIEKYKEV
HHCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MKIGNVEIKGKLVQGPMAGVSNPPFRLISKEKGADLVCAEMVSVEGMTHNNKKTFSMLAV
CCCCCEEEEEEEEECCCCCCCCCCCEEEECCCCCCHHHHHHHHHCCCCCCCCCEEEEEEE
EKKEHPMSMQIFGNDVESFIVATKIDENVDCDIIDLNIGCPAPKVAVRSASGSSLLKTPE
HHCCCCCEEEEECCCHHHEEEEEECCCCCCCEEEEEECCCCCCHHEEECCCCCCHHCCHH
LIYDIVKAVVENTSKPVTAKIRLGDKESVNAVEVSKLIEKAGASGITVHGRTRSEFYSGH
HHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEECCCHHHHHCCC
ADEKIKEVKEAVNIPVIGNGDVIDGPSAKAMLELTGCDGVMISRGCQGNPIFREIAHFLE
CHHHHHHHHHHHCCCEECCCCEECCCCCCEEEEEECCCCEEECCCCCCCHHHHHHHHHHH
TGEELDKPSFAEKETVLKHAKMLIDYKQDEGSAIREFRKHLTYFAALPKTEILNQLKEEA
CHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
NKINTFSDLENIIEKYKEV
HHCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]