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The map label for this gene is lysS [H]

Identifier: 50364845

GI number: 50364845

Start: 41444

End: 42943

Strand: Direct

Name: lysS [H]

Synonym: Mfl030

Alternate gene names: 50364845

Gene position: 41444-42943 (Clockwise)

Preceding gene: 50364844

Following gene: 50364847

Centisome position: 5.22

GC content: 31.0

Gene sequence:

>1500_bases
ATGTCAGAAAGAAAATTTACTGAACAAGAATTAGTTCGTAGAGCTAAACTTGAAAAATTAATTGAAAACAAATCTAATCC
TTATGAAGTTAGCAAGTATGTAAGAACACATTCTTTAAAAGAATTGATTGAATTATTTGGTGGATATGAAAAAGATGAGT
TAGCAACTTTACAACAAACAGAAATTAGCATTGCAGGAAGAATTAAACTTTATCGTGAGGCTGGTAATAAAGCAGCATTC
TTAAATATTGAAGATCAAGATTCAGATATTCAAGTTTATGTAAGACAAGACGAAATTGGTGATGCAGCATTTGCTAACTT
CAGAGAACTAGATCTTGGAGATATTGTTGGGTTTAAAGGAATTATGATGAAAACAAACCATGGTGAACTTTCACTAAGAG
CAAAAGAATATACTTTATTATCAAAAGCTTTAAGACCTTTACCAGATAAACATGCTGGTATTCAAGACATTGAAGAAAAA
TACAGAAGAAGATATGTTGACTTAATCACAAACCCTGAATCAAAAAGAGTTTTTCAAGCAAGAACTAAAATTATTAGAAC
AATGCAACAAATTCTTGATGAACGTGGGTATATGGAAGTTGAAACACCTATTTTGCACAGTGTAAAAGGTGGAGCCGCTG
CTAAACCTTTTATTACACATTACAATGCTTTAGATGTTGATGTATATTTAAGAATTGCAACAGAGCTACATTTAAAAAGA
TTAATTGTTGGTGGATTTGATGGAGTTTATGAAATTGGAAGAATCTTTAGAAATGAAGGAATGAGCACAAGACACAATCC
AGAATTTACTTCTTTAGAATTGTATGTTGCATATGAAGATATGTTCTTCTTAATGGACTTAACTGAAGAAATATTCAGAA
AATGTAATGCTGCAGTTAACGAATCATCAGTTATAGAATATGGTGGACATAAACTAGATTTATCAAAACCATTTAAACGC
TTACATATGGTTGATGGAATTAAACAAGTGACAGGAGTAGATTTCTGAAAAGAAATGACTGTTGAAGAAGCTCTAGAGTT
AGCTAAAAAACATAATGTGCATGTTGAAAAACATAATTATACAGTTGGACATATTATTAACTTATTCTATGAAGAATTTG
TTGAGTCTACAATTATTGAACCAACTTTTGTTTATGGACATCCAAAAGAAATATCACCTTTATCTAAATCGAATGCTGAA
GATTCAAGATTCACTGATCGTTTTGAGTTGTTTATTGTCGGAAGAGAATATGCAAATGCATTTAGTGAATTAAATGATCC
AATCGATCAATATGATAGATTTAATGAACAAATAAAAGAAGCTGCGGCGGGAAATGATGAAGCAACTGAAATGGACATTG
ACTTTATTGAAGCGTTGGAACACGCAATGCCACCAACTGCAGGTATCGGATTTGGAATTGATCGTTTAGTTATGTTATTA
ACAAATTCTGAATCAATTAAGGATGTATTATTATTCCCACAGTTAAAACCAAGAGATTAA

Upstream 100 bases:

>100_bases
CCAAAAACAGAAATTTTAAATCAGTTAAAAGAAGAAGCAAATAAGATTAACACATTTAGTGATTTAGAAAATATTATAGA
AAAGTATAAAGAGGTATAAA

Downstream 100 bases:

>100_bases
TATGTTAAAAAACAAACCTTAATGGTTTGTTTTCTTTATGCATTGGCTATACTGTTAGCTTCTTCGTCTAATATTAAGGT
AAAGAAAGGATGTAGTGTTA

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS [H]

Number of amino acids: Translated: 499; Mature: 498

Protein sequence:

>499_residues
MSERKFTEQELVRRAKLEKLIENKSNPYEVSKYVRTHSLKELIELFGGYEKDELATLQQTEISIAGRIKLYREAGNKAAF
LNIEDQDSDIQVYVRQDEIGDAAFANFRELDLGDIVGFKGIMMKTNHGELSLRAKEYTLLSKALRPLPDKHAGIQDIEEK
YRRRYVDLITNPESKRVFQARTKIIRTMQQILDERGYMEVETPILHSVKGGAAAKPFITHYNALDVDVYLRIATELHLKR
LIVGGFDGVYEIGRIFRNEGMSTRHNPEFTSLELYVAYEDMFFLMDLTEEIFRKCNAAVNESSVIEYGGHKLDLSKPFKR
LHMVDGIKQVTGVDFWKEMTVEEALELAKKHNVHVEKHNYTVGHIINLFYEEFVESTIIEPTFVYGHPKEISPLSKSNAE
DSRFTDRFELFIVGREYANAFSELNDPIDQYDRFNEQIKEAAAGNDEATEMDIDFIEALEHAMPPTAGIGFGIDRLVMLL
TNSESIKDVLLFPQLKPRD

Sequences:

>Translated_499_residues
MSERKFTEQELVRRAKLEKLIENKSNPYEVSKYVRTHSLKELIELFGGYEKDELATLQQTEISIAGRIKLYREAGNKAAF
LNIEDQDSDIQVYVRQDEIGDAAFANFRELDLGDIVGFKGIMMKTNHGELSLRAKEYTLLSKALRPLPDKHAGIQDIEEK
YRRRYVDLITNPESKRVFQARTKIIRTMQQILDERGYMEVETPILHSVKGGAAAKPFITHYNALDVDVYLRIATELHLKR
LIVGGFDGVYEIGRIFRNEGMSTRHNPEFTSLELYVAYEDMFFLMDLTEEIFRKCNAAVNESSVIEYGGHKLDLSKPFKR
LHMVDGIKQVTGVDF*KEMTVEEALELAKKHNVHVEKHNYTVGHIINLFYEEFVESTIIEPTFVYGHPKEISPLSKSNAE
DSRFTDRFELFIVGREYANAFSELNDPIDQYDRFNEQIKEAAAGNDEATEMDIDFIEALEHAMPPTAGIGFGIDRLVMLL
TNSESIKDVLLFPQLKPRD
>Mature_498_residues
SERKFTEQELVRRAKLEKLIENKSNPYEVSKYVRTHSLKELIELFGGYEKDELATLQQTEISIAGRIKLYREAGNKAAFL
NIEDQDSDIQVYVRQDEIGDAAFANFRELDLGDIVGFKGIMMKTNHGELSLRAKEYTLLSKALRPLPDKHAGIQDIEEKY
RRRYVDLITNPESKRVFQARTKIIRTMQQILDERGYMEVETPILHSVKGGAAAKPFITHYNALDVDVYLRIATELHLKRL
IVGGFDGVYEIGRIFRNEGMSTRHNPEFTSLELYVAYEDMFFLMDLTEEIFRKCNAAVNESSVIEYGGHKLDLSKPFKRL
HMVDGIKQVTGVDF*KEMTVEEALELAKKHNVHVEKHNYTVGHIINLFYEEFVESTIIEPTFVYGHPKEISPLSKSNAED
SRFTDRFELFIVGREYANAFSELNDPIDQYDRFNEQIKEAAAGNDEATEMDIDFIEALEHAMPPTAGIGFGIDRLVMLLT
NSESIKDVLLFPQLKPRD

Specific function: Unknown

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI194272210, Length=502, Percent_Identity=39.8406374501992, Blast_Score=354, Evalue=1e-97,
Organism=Homo sapiens, GI5031815, Length=502, Percent_Identity=39.8406374501992, Blast_Score=353, Evalue=2e-97,
Organism=Homo sapiens, GI45439306, Length=475, Percent_Identity=20.2105263157895, Blast_Score=76, Evalue=6e-14,
Organism=Escherichia coli, GI1789256, Length=489, Percent_Identity=47.4437627811861, Blast_Score=445, Evalue=1e-126,
Organism=Escherichia coli, GI1790571, Length=468, Percent_Identity=47.6495726495726, Blast_Score=445, Evalue=1e-126,
Organism=Escherichia coli, GI87082379, Length=321, Percent_Identity=30.5295950155763, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI1788173, Length=291, Percent_Identity=23.0240549828179, Blast_Score=74, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17535925, Length=490, Percent_Identity=42.8571428571429, Blast_Score=384, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI17535927, Length=490, Percent_Identity=42.8571428571429, Blast_Score=384, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI71994340, Length=490, Percent_Identity=42.8571428571429, Blast_Score=383, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6320242, Length=495, Percent_Identity=40.8080808080808, Blast_Score=360, Evalue=1e-100,
Organism=Saccharomyces cerevisiae, GI6324256, Length=478, Percent_Identity=32.2175732217573, Blast_Score=205, Evalue=1e-53,
Organism=Saccharomyces cerevisiae, GI6323011, Length=477, Percent_Identity=24.1090146750524, Blast_Score=85, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24640851, Length=504, Percent_Identity=40.0793650793651, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24640849, Length=504, Percent_Identity=40.0793650793651, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI17136276, Length=468, Percent_Identity=23.0769230769231, Blast_Score=87, Evalue=3e-17,

Paralogues:

None

Copy number: 1200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.6 [H]

Molecular weight: Translated: 57241; Mature: 57110

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSERKFTEQELVRRAKLEKLIENKSNPYEVSKYVRTHSLKELIELFGGYEKDELATLQQT
CCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
EISIAGRIKLYREAGNKAAFLNIEDQDSDIQVYVRQDEIGDAAFANFRELDLGDIVGFKG
HEEHHHHEEEEECCCCEEEEEEECCCCCCEEEEEEECCCCCHHHCCCCCCCHHHHCCCCC
IMMKTNHGELSLRAKEYTLLSKALRPLPDKHAGIQDIEEKYRRRYVDLITNPESKRVFQA
EEEECCCCCEEEEHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHH
RTKIIRTMQQILDERGYMEVETPILHSVKGGAAAKPFITHYNALDVDVYLRIATELHLKR
HHHHHHHHHHHHHHCCCEEECCHHHHHCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHH
LIVGGFDGVYEIGRIFRNEGMSTRHNPEFTSLELYVAYEDMFFLMDLTEEIFRKCNAAVN
HHHCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEHHHHHHHHHHHHHHHHHCCCCC
ESSVIEYGGHKLDLSKPFKRLHMVDGIKQVTGVDFKEMTVEEALELAKKHNVHVEKHNYT
CCCHHHCCCCEECHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEECCCH
VGHIINLFYEEFVESTIIEPTFVYGHPKEISPLSKSNAEDSRFTDRFELFIVGREYANAF
HHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCHHCCCEEEEEEEEHHHHHHH
SELNDPIDQYDRFNEQIKEAAAGNDEATEMDIDFIEALEHAMPPTAGIGFGIDRLVMLLT
HHHCCHHHHHHHHHHHHHHHHCCCCCCHHCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHC
NSESIKDVLLFPQLKPRD
CCCHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SERKFTEQELVRRAKLEKLIENKSNPYEVSKYVRTHSLKELIELFGGYEKDELATLQQT
CCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
EISIAGRIKLYREAGNKAAFLNIEDQDSDIQVYVRQDEIGDAAFANFRELDLGDIVGFKG
HEEHHHHEEEEECCCCEEEEEEECCCCCCEEEEEEECCCCCHHHCCCCCCCHHHHCCCCC
IMMKTNHGELSLRAKEYTLLSKALRPLPDKHAGIQDIEEKYRRRYVDLITNPESKRVFQA
EEEECCCCCEEEEHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHH
RTKIIRTMQQILDERGYMEVETPILHSVKGGAAAKPFITHYNALDVDVYLRIATELHLKR
HHHHHHHHHHHHHHCCCEEECCHHHHHCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHH
LIVGGFDGVYEIGRIFRNEGMSTRHNPEFTSLELYVAYEDMFFLMDLTEEIFRKCNAAVN
HHHCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEHHHHHHHHHHHHHHHHHCCCCC
ESSVIEYGGHKLDLSKPFKRLHMVDGIKQVTGVDFKEMTVEEALELAKKHNVHVEKHNYT
CCCHHHCCCCEECHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEECCCH
VGHIINLFYEEFVESTIIEPTFVYGHPKEISPLSKSNAEDSRFTDRFELFIVGREYANAF
HHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCHHCCCEEEEEEEEHHHHHHH
SELNDPIDQYDRFNEQIKEAAAGNDEATEMDIDFIEALEHAMPPTAGIGFGIDRLVMLLT
HHHCCHHHHHHHHHHHHHHHHCCCCCCHHCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHC
NSESIKDVLLFPQLKPRD
CCCHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA