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The map label for this gene is bglH [H]

Identifier: 50364827

GI number: 50364827

Start: 16264

End: 17718

Strand: Direct

Name: bglH [H]

Synonym: Mfl012

Alternate gene names: 50364827

Gene position: 16264-17718 (Clockwise)

Preceding gene: 50364826

Following gene: 50364828

Centisome position: 2.05

GC content: 27.77

Gene sequence:

>1455_bases
ATGACTAAAAAATTAGAATTTCCAAGCAATTTTTTATGAGGTGGTGCTACTGCAGCATCTCAAATAGAAGGTGCTTATAA
TGTAGATGGAAAATCTCTTTCTGCTATTGAAATGATACCGTTTATTGAATTAAAAGATAGAAAAGATTTATCACAAACAA
GAAAGTTAACAACCCAAGATTTACTTGACTCTATTGAAAATAAAAAAGGTTTACATTACCCTAAAAGATTTGGAATAGAC
TTTTATCATAAATATAAAGAAGATATTGCTTTGTTTAAAGAAGCTGGAATGAAAGTGTTTAGAATGTCAGTTGCATGAAC
ACGAATTTTTCCCAATGGAGATGAAACAGAACCTAATAGAAAAGGTCTAGAATTTTATAAAAACGTTTTTGAAGAATGCA
AAAAACAAGGTTTAGAAATAATGGTAACTATAAATCATTTTGACATGCCTTTTTCAATAATTCAAAAATATGGCGGATGA
AAAAATAGACAAGTAATTGATATGTATTTTAAATACTCAAAAACTTTAATAGACGAATTTCATAGCTATGTTAGATACTG
ATTACCTTTTAACGAAATCAATTTAGGTGTTTTATTTTCTGCAATGGGATTAGAGGGTAATAACGAAGAAAAAAGTTTAG
CGGAAAGATTAGCAGGAACTTATCAGGATCTTCATAATCAATTTGTAGCGCAAGCAAAATTCATAGAGTATTCAAAAAAA
TTTAGTAATACAAAAGTTGGGTGTATGGTAGCAGATACAACAACATATGCAATTGATTGTAATCCAGTTAATGTTTTAGA
AAATCAAAGAAAAGAACAAATGAAAAAGTTTTTTTACTATGATGTTATGACAAAAGGAGAATATCCAAGCTATTCAAAAA
GATACTTTAAAGAAAATGGGATTAATATTGTTAAAGAAAAAACAGATGATGAATTACTTAAAAATAATACAGTTGAATTT
TTATCATTCAGTTATTATATGTCTAGCACAATTGCTAAAGAAGAAGTTGAATTAACCGAGGGTAATTTAATGAAAATGGG
AAAAAATCCGTTTTTAGTTGCAACTGAATGAGGATGACAAATTGATCCTATTGGACTTAGATATTGCTTAAATCAGTTAT
GAGACAGATATCATTTACCTTTATTTATTTCTGAAAATGGTATGGGTGTTTTAGAAACATTAAATGAAAATAATACTGTA
GATGATGATTATCGAATTGAATATTTGAGTAGACACTTTGAACAAATGAGCGAAGCTATTAAAGACGGAGTAAATTTATT
TGGATATACAATGTGAACACCTATTGATGTTGTTAGTGCAGGATCAAATGAAATGTCAAAAAGATATGGATTAATATTTG
TAGATTATGATGATTATCATAGAGGAACAGGGAATAGATTTAAGAAAAAATCTTTTACTTGATTCCAAAACTTTATGAAA
ACAAGCGAAGTTTAA

Upstream 100 bases:

>100_bases
TGATTTAAAAGATATGAAAAGATATAAAAAAGATAGTTTTCATTGATATAAAGAATTAATTAAAAATAATGGTGAAAATA
TTTAGAGGAGATAGATACAT

Downstream 100 bases:

>100_bases
GGAGATAAAATGAAATATTATACTTTTGATATAGGTGGAACATCTACAAAGTACCTAATTTTTGAAGATAAAAAAGTTAT
TAAATCTGGCGTAATTGAGT

Product: beta-glucosidase

Products: NA

Alternate protein names: 6-phospho-beta-glucosidase [H]

Number of amino acids: Translated: 484; Mature: 483

Protein sequence:

>484_residues
MTKKLEFPSNFLWGGATAASQIEGAYNVDGKSLSAIEMIPFIELKDRKDLSQTRKLTTQDLLDSIENKKGLHYPKRFGID
FYHKYKEDIALFKEAGMKVFRMSVAWTRIFPNGDETEPNRKGLEFYKNVFEECKKQGLEIMVTINHFDMPFSIIQKYGGW
KNRQVIDMYFKYSKTLIDEFHSYVRYWLPFNEINLGVLFSAMGLEGNNEEKSLAERLAGTYQDLHNQFVAQAKFIEYSKK
FSNTKVGCMVADTTTYAIDCNPVNVLENQRKEQMKKFFYYDVMTKGEYPSYSKRYFKENGINIVKEKTDDELLKNNTVEF
LSFSYYMSSTIAKEEVELTEGNLMKMGKNPFLVATEWGWQIDPIGLRYCLNQLWDRYHLPLFISENGMGVLETLNENNTV
DDDYRIEYLSRHFEQMSEAIKDGVNLFGYTMWTPIDVVSAGSNEMSKRYGLIFVDYDDYHRGTGNRFKKKSFTWFQNFMK
TSEV

Sequences:

>Translated_484_residues
MTKKLEFPSNFL*GGATAASQIEGAYNVDGKSLSAIEMIPFIELKDRKDLSQTRKLTTQDLLDSIENKKGLHYPKRFGID
FYHKYKEDIALFKEAGMKVFRMSVA*TRIFPNGDETEPNRKGLEFYKNVFEECKKQGLEIMVTINHFDMPFSIIQKYGG*
KNRQVIDMYFKYSKTLIDEFHSYVRY*LPFNEINLGVLFSAMGLEGNNEEKSLAERLAGTYQDLHNQFVAQAKFIEYSKK
FSNTKVGCMVADTTTYAIDCNPVNVLENQRKEQMKKFFYYDVMTKGEYPSYSKRYFKENGINIVKEKTDDELLKNNTVEF
LSFSYYMSSTIAKEEVELTEGNLMKMGKNPFLVATE*G*QIDPIGLRYCLNQL*DRYHLPLFISENGMGVLETLNENNTV
DDDYRIEYLSRHFEQMSEAIKDGVNLFGYTM*TPIDVVSAGSNEMSKRYGLIFVDYDDYHRGTGNRFKKKSFT*FQNFMK
TSEV
>Mature_483_residues
TKKLEFPSNFL*GGATAASQIEGAYNVDGKSLSAIEMIPFIELKDRKDLSQTRKLTTQDLLDSIENKKGLHYPKRFGIDF
YHKYKEDIALFKEAGMKVFRMSVA*TRIFPNGDETEPNRKGLEFYKNVFEECKKQGLEIMVTINHFDMPFSIIQKYGG*K
NRQVIDMYFKYSKTLIDEFHSYVRY*LPFNEINLGVLFSAMGLEGNNEEKSLAERLAGTYQDLHNQFVAQAKFIEYSKKF
SNTKVGCMVADTTTYAIDCNPVNVLENQRKEQMKKFFYYDVMTKGEYPSYSKRYFKENGINIVKEKTDDELLKNNTVEFL
SFSYYMSSTIAKEEVELTEGNLMKMGKNPFLVATE*G*QIDPIGLRYCLNQL*DRYHLPLFISENGMGVLETLNENNTVD
DDYRIEYLSRHFEQMSEAIKDGVNLFGYTM*TPIDVVSAGSNEMSKRYGLIFVDYDDYHRGTGNRFKKKSFT*FQNFMKT
SEV

Specific function: Catalyzes the hydrolysis of aryl-phospho-beta-D- glucosides such as 4-methylumbelliferyl-phospho-beta-D- glucopyranoside (MUG-P), phosphoarbutin and phosphosalicin. Plays a major role in the utilization of arbutin or salicin as the sole carbon source. Bgl

COG id: COG2723

COG function: function code G; Beta-glucosidase/6-phospho-beta-glucosidase/beta-galactosidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 1 family [H]

Homologues:

Organism=Homo sapiens, GI110681710, Length=494, Percent_Identity=24.4939271255061, Blast_Score=112, Evalue=1e-24,
Organism=Homo sapiens, GI13273313, Length=476, Percent_Identity=24.3697478991597, Blast_Score=110, Evalue=4e-24,
Organism=Homo sapiens, GI32481206, Length=473, Percent_Identity=24.3128964059197, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI28376633, Length=461, Percent_Identity=24.0780911062907, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI24497614, Length=461, Percent_Identity=24.9457700650759, Blast_Score=86, Evalue=5e-17,
Organism=Escherichia coli, GI1789070, Length=476, Percent_Identity=43.2773109243698, Blast_Score=358, Evalue=1e-100,
Organism=Escherichia coli, GI2367270, Length=473, Percent_Identity=41.014799154334, Blast_Score=348, Evalue=3e-97,
Organism=Escherichia coli, GI2367174, Length=496, Percent_Identity=38.1048387096774, Blast_Score=310, Evalue=1e-85,
Organism=Caenorhabditis elegans, GI17539390, Length=508, Percent_Identity=25.5905511811024, Blast_Score=135, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI17552856, Length=501, Percent_Identity=24.9500998003992, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI21356577, Length=507, Percent_Identity=20.7100591715976, Blast_Score=89, Evalue=8e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001360
- InterPro:   IPR018120
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00232 Glyco_hydro_1 [H]

EC number: =3.2.1.86 [H]

Molecular weight: Translated: 54851; Mature: 54720

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS00018 EF_HAND_1 ; PS00572 GLYCOSYL_HYDROL_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKKLEFPSNFLGGATAASQIEGAYNVDGKSLSAIEMIPFIELKDRKDLSQTRKLTTQDL
CCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCEEEECCEEECCCCHHHHHHHHHHHHH
LDSIENKKGLHYPKRFGIDFYHKYKEDIALFKEAGMKVFRMSVATRIFPNGDETEPNRKG
HHHHHCCCCCCCCHHHCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCHH
LEFYKNVFEECKKQGLEIMVTINHFDMPFSIIQKYGGKNRQVIDMYFKYSKTLIDEFHSY
HHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHH
VRYLPFNEINLGVLFSAMGLEGNNEEKSLAERLAGTYQDLHNQFVAQAKFIEYSKKFSNT
HHHCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
KVGCMVADTTTYAIDCNPVNVLENQRKEQMKKFFYYDVMTKGEYPSYSKRYFKENGINIV
EEEEEEECCEEEEEECCCHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHCCCEEE
KEKTDDELLKNNTVEFLSFSYYMSSTIAKEEVELTEGNLMKMGKNPFLVATEGQIDPIGL
ECCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEEECCCCCCHHH
RYCLNQLDRYHLPLFISENGMGVLETLNENNTVDDDYRIEYLSRHFEQMSEAIKDGVNLF
HHHHHHHCCCCCCEEECCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEE
GYTMTPIDVVSAGSNEMSKRYGLIFVDYDDYHRGTGNRFKKKSFTFQNFMKTSEV
EEEECCHHHCCCCCHHHHHHCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TKKLEFPSNFLGGATAASQIEGAYNVDGKSLSAIEMIPFIELKDRKDLSQTRKLTTQDL
CCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCEEEECCEEECCCCHHHHHHHHHHHHH
LDSIENKKGLHYPKRFGIDFYHKYKEDIALFKEAGMKVFRMSVATRIFPNGDETEPNRKG
HHHHHCCCCCCCCHHHCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCHH
LEFYKNVFEECKKQGLEIMVTINHFDMPFSIIQKYGGKNRQVIDMYFKYSKTLIDEFHSY
HHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHH
VRYLPFNEINLGVLFSAMGLEGNNEEKSLAERLAGTYQDLHNQFVAQAKFIEYSKKFSNT
HHHCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
KVGCMVADTTTYAIDCNPVNVLENQRKEQMKKFFYYDVMTKGEYPSYSKRYFKENGINIV
EEEEEEECCEEEEEECCCHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHCCCEEE
KEKTDDELLKNNTVEFLSFSYYMSSTIAKEEVELTEGNLMKMGKNPFLVATEGQIDPIGL
ECCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEEECCCCCCHHH
RYCLNQLDRYHLPLFISENGMGVLETLNENNTVDDDYRIEYLSRHFEQMSEAIKDGVNLF
HHHHHHHCCCCCCEEECCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEE
GYTMTPIDVVSAGSNEMSKRYGLIFVDYDDYHRGTGNRFKKKSFTFQNFMKTSEV
EEEECCHHHCCCCCHHHHHHCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7883710; 7704263; 9384377 [H]