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The map label for this gene is ksgA

Identifier: 50364820

GI number: 50364820

Start: 4280

End: 5083

Strand: Direct

Name: ksgA

Synonym: Mfl005

Alternate gene names: 50364820

Gene position: 4280-5083 (Clockwise)

Preceding gene: 50364819

Following gene: 50364821

Centisome position: 0.54

GC content: 24.38

Gene sequence:

>804_bases
ATGAAAGTTGAAGCAAAGAAAAAATTTGGTCAAAACTTTATTAGTGATCAAAATTTAATTAATAAGATAGTTTCAATATT
AGGTAATGATAAAGATCAACTAATTATAGAAATTGGTCCAGGAACAGGAGCACTAACTAAATTACTTGCACAAAAATATA
ACAAAGTAGTAGCAATAGAAATTGATACTGACATGGAGCCTATTTTAAAAAAAGAAATAACAAATGACAATTTCGAATTA
TTTCTTTCAGATGTGTTATTAGTTGATTTTGAAAAATTAATAAAAGAAAAAAGACAACATGAAAATCAAAAAGTTTCAAT
AATTTCTAACATGCCTTACTATATAACAAGTGAAATACTTTTTAGAACATTAAATGTAAGTGATAAGTTAACAAAAGCTG
TTTTTATGATGCAAAAAGAAGTTGCTATTAGAGTGTGTTCATATAAAGGCGAAAATAATTACAATAATCTTTCAGTTGCT
TGTGAATTTTATGCAGATAAAAAATATGAGTTTACAGTTCCTAAACATATGTTCTACCCAGTACCGAAAGTGGATTCTGC
AATAATATCATTAACATTCAACAATAAATACACTGAACAAATTAAAGACAAAGATAAATTCTTAACATTTTTAAGAAAAA
TATTTAATAACAGAAGAAAAACAATATTGAATAATTTATCAAATGTAACTAATGACAAAACAAAAGCTAATGAAATCCTT
GATAATCTTAATATTGATAAATCATTAAGACCTGAAGTAGTTGGTTTAGAAGACTTTATTAGAATTTACAACAAAACTAG
ATAA

Upstream 100 bases:

>100_bases
TTTAATTTGAGTGAAGATATGAGTTCAAAGAGTTTATTCAAATGATTAAATTGAATGAATTTAAAAGTTAAAGATATAGA
AAAAATAATAGGTGAGTAGA

Downstream 100 bases:

>100_bases
TATTTTGTAATTAAAAGTGGCTTCTTGCTACTTTTTTAATTTTTATTTGTGCTAAAAACTTGAAATAAAGCAATTTAAGG
GCATTTTTTAAGCATTTTAT

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MKVEAKKKFGQNFISDQNLINKIVSILGNDKDQLIIEIGPGTGALTKLLAQKYNKVVAIEIDTDMEPILKKEITNDNFEL
FLSDVLLVDFEKLIKEKRQHENQKVSIISNMPYYITSEILFRTLNVSDKLTKAVFMMQKEVAIRVCSYKGENNYNNLSVA
CEFYADKKYEFTVPKHMFYPVPKVDSAIISLTFNNKYTEQIKDKDKFLTFLRKIFNNRRKTILNNLSNVTNDKTKANEIL
DNLNIDKSLRPEVVGLEDFIRIYNKTR

Sequences:

>Translated_267_residues
MKVEAKKKFGQNFISDQNLINKIVSILGNDKDQLIIEIGPGTGALTKLLAQKYNKVVAIEIDTDMEPILKKEITNDNFEL
FLSDVLLVDFEKLIKEKRQHENQKVSIISNMPYYITSEILFRTLNVSDKLTKAVFMMQKEVAIRVCSYKGENNYNNLSVA
CEFYADKKYEFTVPKHMFYPVPKVDSAIISLTFNNKYTEQIKDKDKFLTFLRKIFNNRRKTILNNLSNVTNDKTKANEIL
DNLNIDKSLRPEVVGLEDFIRIYNKTR
>Mature_267_residues
MKVEAKKKFGQNFISDQNLINKIVSILGNDKDQLIIEIGPGTGALTKLLAQKYNKVVAIEIDTDMEPILKKEITNDNFEL
FLSDVLLVDFEKLIKEKRQHENQKVSIISNMPYYITSEILFRTLNVSDKLTKAVFMMQKEVAIRVCSYKGENNYNNLSVA
CEFYADKKYEFTVPKHMFYPVPKVDSAIISLTFNNKYTEQIKDKDKFLTFLRKIFNNRRKTILNNLSNVTNDKTKANEIL
DNLNIDKSLRPEVVGLEDFIRIYNKTR

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI156415992, Length=279, Percent_Identity=27.2401433691756, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI7657198, Length=211, Percent_Identity=27.9620853080569, Blast_Score=85, Evalue=7e-17,
Organism=Escherichia coli, GI1786236, Length=266, Percent_Identity=30.8270676691729, Blast_Score=150, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI25146882, Length=222, Percent_Identity=28.8288288288288, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI25141369, Length=283, Percent_Identity=23.321554770318, Blast_Score=75, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6324989, Length=223, Percent_Identity=28.2511210762332, Blast_Score=91, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21357273, Length=290, Percent_Identity=25.1724137931034, Blast_Score=100, Evalue=9e-22,
Organism=Drosophila melanogaster, GI21358017, Length=212, Percent_Identity=30.6603773584906, Blast_Score=86, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_MESFL (Q6F2B4)

Other databases:

- EMBL:   AE017263
- RefSeq:   YP_053245.1
- ProteinModelPortal:   Q6F2B4
- SMR:   Q6F2B4
- GeneID:   2898270
- GenomeReviews:   AE017263_GR
- KEGG:   mfl:Mfl005
- HOGENOM:   HBG319664
- OMA:   GVLCGWR
- ProtClustDB:   PRK00274
- BioCyc:   MFLO265311:MFL005-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.182

Molecular weight: Translated: 31027; Mature: 31027

Theoretical pI: Translated: 9.59; Mature: 9.59

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 12-12 BINDING 14-14 BINDING 39-39 BINDING 60-60 BINDING 84-84 BINDING 110-110

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVEAKKKFGQNFISDQNLINKIVSILGNDKDQLIIEIGPGTGALTKLLAQKYNKVVAIE
CCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHCCCEEEEE
IDTDMEPILKKEITNDNFELFLSDVLLVDFEKLIKEKRQHENQKVSIISNMPYYITSEIL
ECCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHH
FRTLNVSDKLTKAVFMMQKEVAIRVCSYKGENNYNNLSVACEFYADKKYEFTVPKHMFYP
HHHCCCHHHHHHHHHHHHHHHHHHHEECCCCCCCCCEEEEEEEECCCEEEEECCHHHCCC
VPKVDSAIISLTFNNKYTEQIKDKDKFLTFLRKIFNNRRKTILNNLSNVTNDKTKANEIL
CCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
DNLNIDKSLRPEVVGLEDFIRIYNKTR
HHCCCCCCCCCCEECHHHHHHHHHCCC
>Mature Secondary Structure
MKVEAKKKFGQNFISDQNLINKIVSILGNDKDQLIIEIGPGTGALTKLLAQKYNKVVAIE
CCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHCCCEEEEE
IDTDMEPILKKEITNDNFELFLSDVLLVDFEKLIKEKRQHENQKVSIISNMPYYITSEIL
ECCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHH
FRTLNVSDKLTKAVFMMQKEVAIRVCSYKGENNYNNLSVACEFYADKKYEFTVPKHMFYP
HHHCCCHHHHHHHHHHHHHHHHHHHEECCCCCCCCCEEEEEEEECCCEEEEECCHHHCCC
VPKVDSAIISLTFNNKYTEQIKDKDKFLTFLRKIFNNRRKTILNNLSNVTNDKTKANEIL
CCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
DNLNIDKSLRPEVVGLEDFIRIYNKTR
HHCCCCCCCCCCEECHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA