Click here to switch to the map view.

The map label for this gene is dnaN [H]

Identifier: 50364817

GI number: 50364817

Start: 1560

End: 2681

Strand: Direct

Name: dnaN [H]

Synonym: Mfl002

Alternate gene names: 50364817

Gene position: 1560-2681 (Clockwise)

Preceding gene: 50364816

Following gene: 50364819

Centisome position: 0.2

GC content: 22.99

Gene sequence:

>1122_bases
ATGAGATTAAGTATAAATAAATCAGTTCTATTAAATGAATTAACAAAGGCAAGTAAATTAATTGAACTTAAAAATGTTAA
CCCAGCATTACAAGGGGTTTATATGGAAGTTAGTTTTGATAAACTTGTTATAGTTTCATCAAACACGTCTACATCTTTTA
AAGCTGAATTAAATAATGAAAACTCAGATTTAATCATAGAACAACCAGGACGTATATTAGTTAAACCTAAATTTATTTTA
GAATTACTTAGAAAGTTAGACAGTGAGTTTGTAACACTTTCTACATTTGCTGAAAATGAATTAGAAATAATAACAGAAAA
ATCAAATTTAAAAGTTTCTATATTAAATGTAGAAGAATTCCCATTAATTGGTTTTGTTGAAAATGGTTTAGAATTATCTA
TTGATTCTCAAGAATTTAAAAGCACACTTACTCAAACTATTAGTTCAATTAATGAATGAAACCAAAAAGTTGTTTTAGCT
GGAATGAATTTAAAAATAAAAGATAACAAAATATCATTTGTTACAACTGACTTATTTAGAGTTAGTTTAAAAGAAATCAT
TTTAAATGAAGCAACTAATCAGGAAGTAGATATTATTATTCCATACAAAACTTTAATAGAATTAAGAAACTTAATAGAAA
ACGTTAAAGAATTTAAAATTATAATACATGATACTAATGCAACATTTAAATTAGATAATGATTTATTACAATCTACTTTA
ATTGATGGAAGATATCCAAATGTTCATTCAGCATTCCCTACAACACATGAAATAAAATTAGAATTAAAAGCTAAAACATT
ATTAAAAGTTTTAAGTAGATTTGAATTAACAAACGATCAAAACATTACTTCAGTTGTTAATTTAGAAATTCAACAAAGTG
GAATTATATTAAAAACAACAATTGCTGAAACAGTTAAATATGAAGAAAAATTTACAGAATATAAATATGAAGGTTCAAGT
AATTTAAATATTAACTTTAATACAAAATATTTAATTGAAGCTATAAGAAGTTTTGATGATCAACTAATTCATTTAACTTT
TAATTCTCAAAATAAACCTGTATTAATAACAGGAGCACAAAAAAGAGATTTGAAACAAGTAGTTTTACCAACTTATGCAT
AA

Upstream 100 bases:

>100_bases
ACTTATATAAAGAATAATATAATATATAAAAGCATGCGTATTTCTTTTGTTTTTATTTTATAAATATGAGATAATATATA
TTGTAAATAAGAGGTGTTAT

Downstream 100 bases:

>100_bases
TAAAAAAATCAGGAAATTTCCTGATTTTATTTTTTTGTATTATTAAACTTTAAAGAACCTTCAATATCTGAATATCCACG
TTTTTTTGAAATAGTAACTG

Product: DNA polymerase III, beta chain

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MRLSINKSVLLNELTKASKLIELKNVNPALQGVYMEVSFDKLVIVSSNTSTSFKAELNNENSDLIIEQPGRILVKPKFIL
ELLRKLDSEFVTLSTFAENELEIITEKSNLKVSILNVEEFPLIGFVENGLELSIDSQEFKSTLTQTISSINEWNQKVVLA
GMNLKIKDNKISFVTTDLFRVSLKEIILNEATNQEVDIIIPYKTLIELRNLIENVKEFKIIIHDTNATFKLDNDLLQSTL
IDGRYPNVHSAFPTTHEIKLELKAKTLLKVLSRFELTNDQNITSVVNLEIQQSGIILKTTIAETVKYEEKFTEYKYEGSS
NLNINFNTKYLIEAIRSFDDQLIHLTFNSQNKPVLITGAQKRDLKQVVLPTYA

Sequences:

>Translated_373_residues
MRLSINKSVLLNELTKASKLIELKNVNPALQGVYMEVSFDKLVIVSSNTSTSFKAELNNENSDLIIEQPGRILVKPKFIL
ELLRKLDSEFVTLSTFAENELEIITEKSNLKVSILNVEEFPLIGFVENGLELSIDSQEFKSTLTQTISSINE*NQKVVLA
GMNLKIKDNKISFVTTDLFRVSLKEIILNEATNQEVDIIIPYKTLIELRNLIENVKEFKIIIHDTNATFKLDNDLLQSTL
IDGRYPNVHSAFPTTHEIKLELKAKTLLKVLSRFELTNDQNITSVVNLEIQQSGIILKTTIAETVKYEEKFTEYKYEGSS
NLNINFNTKYLIEAIRSFDDQLIHLTFNSQNKPVLITGAQKRDLKQVVLPTYA
>Mature_373_residues
MRLSINKSVLLNELTKASKLIELKNVNPALQGVYMEVSFDKLVIVSSNTSTSFKAELNNENSDLIIEQPGRILVKPKFIL
ELLRKLDSEFVTLSTFAENELEIITEKSNLKVSILNVEEFPLIGFVENGLELSIDSQEFKSTLTQTISSINE*NQKVVLA
GMNLKIKDNKISFVTTDLFRVSLKEIILNEATNQEVDIIIPYKTLIELRNLIENVKEFKIIIHDTNATFKLDNDLLQSTL
IDGRYPNVHSAFPTTHEIKLELKAKTLLKVLSRFELTNDQNITSVVNLEIQQSGIILKTTIAETVKYEEKFTEYKYEGSS
NLNINFNTKYLIEAIRSFDDQLIHLTFNSQNKPVLITGAQKRDLKQVVLPTYA

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=372, Percent_Identity=19.6236559139785, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 42299; Mature: 42299

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLSINKSVLLNELTKASKLIELKNVNPALQGVYMEVSFDKLVIVSSNTSTSFKAELNNE
CEEECCHHHHHHHHHHHHHEEEECCCCHHHCEEEEEEEECEEEEEECCCCCEEEEEECCC
NSDLIIEQPGRILVKPKFILELLRKLDSEFVTLSTFAENELEIITEKSNLKVSILNVEEF
CCCEEEECCCCEEECHHHHHHHHHHCCCCEEEEEECCCCCEEEEEECCCCEEEEEEECCC
PLIGFVENGLELSIDSQEFKSTLTQTISSINENQKVVLAGMNLKIKDNKISFVTTDLFRV
CEEEEECCCEEEEECHHHHHHHHHHHHHHCCCCCEEEEEECEEEEECCEEEEEEHHHHHH
SLKEIILNEATNQEVDIIIPYKTLIELRNLIENVKEFKIIIHDTNATFKLDNDLLQSTLI
HHHHHHHCCCCCCCEEEEECHHHHHHHHHHHHHHHEEEEEEEECCCEEEECHHHHHHHHC
DGRYPNVHSAFPTTHEIKLELKAKTLLKVLSRFELTNDQNITSVVNLEIQQSGIILKTTI
CCCCCCCCCCCCCCEEEEEEEEHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCEEEEEHH
AETVKYEEKFTEYKYEGSSNLNINFNTKYLIEAIRSFDDQLIHLTFNSQNKPVLITGAQK
HHHHHHHHHHHHEEECCCCEEEEEECHHHHHHHHHCCCCEEEEEEECCCCCEEEEECCCC
RDLKQVVLPTYA
CCCHHHCCCCCC
>Mature Secondary Structure
MRLSINKSVLLNELTKASKLIELKNVNPALQGVYMEVSFDKLVIVSSNTSTSFKAELNNE
CEEECCHHHHHHHHHHHHHEEEECCCCHHHCEEEEEEEECEEEEEECCCCCEEEEEECCC
NSDLIIEQPGRILVKPKFILELLRKLDSEFVTLSTFAENELEIITEKSNLKVSILNVEEF
CCCEEEECCCCEEECHHHHHHHHHHCCCCEEEEEECCCCCEEEEEECCCCEEEEEEECCC
PLIGFVENGLELSIDSQEFKSTLTQTISSINENQKVVLAGMNLKIKDNKISFVTTDLFRV
CEEEEECCCEEEEECHHHHHHHHHHHHHHCCCCCEEEEEECEEEEECCEEEEEEHHHHHH
SLKEIILNEATNQEVDIIIPYKTLIELRNLIENVKEFKIIIHDTNATFKLDNDLLQSTLI
HHHHHHHCCCCCCCEEEEECHHHHHHHHHHHHHHHEEEEEEEECCCEEEECHHHHHHHHC
DGRYPNVHSAFPTTHEIKLELKAKTLLKVLSRFELTNDQNITSVVNLEIQQSGIILKTTI
CCCCCCCCCCCCCCEEEEEEEEHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCEEEEEHH
AETVKYEEKFTEYKYEGSSNLNINFNTKYLIEAIRSFDDQLIHLTFNSQNKPVLITGAQK
HHHHHHHHHHHHEEECCCCEEEEEECHHHHHHHHHCCCCEEEEEEECCCCCEEEEECCCC
RDLKQVVLPTYA
CCCHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1544573; 8233831 [H]