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The map label for this gene is yafC [H]

Identifier: 50086563

GI number: 50086563

Start: 3526856

End: 3527764

Strand: Reverse

Name: yafC [H]

Synonym: ACIAD3615

Alternate gene names: 50086563

Gene position: 3527764-3526856 (Counterclockwise)

Preceding gene: 50086566

Following gene: 50086561

Centisome position: 98.03

GC content: 42.57

Gene sequence:

>909_bases
GTGAAATCGACTGTAGAAGAGTTAATTGCGTTTATTACCATTGTAGATACAGGTTCGATTGTCGGCGCGGCAGAGCAGTT
GCAGCAAACACCATCTGGCGTGAGTCGTTCTCTGAACCGACTGGAAAAGAAGCTTAATGTCACCTTACTGGAACGAACCA
CGCGTAAAATTAAACTCACCCAAGAGGGCAGTTTATTTTTGATGCGGGCACGGACGATTTTGGCCGATCTATCAGATGCC
GAAGAAGCACTGTTAAAATCGGATAGCGATACCTCAGGTTTGATTCGCATCGATTCTGCCACACCGTTTGTACTGCATGT
ATTGGCCCCCCTGATGCAAAAGTTTATGCAGCTTTATCCACATATCGAGATTGAACTCAATAGTAATGATCAGGTCATTG
ATTTATTGCAGCACAAAACTGATGTTGCGATTCGCTTTGGTGCGCTGCACGATTCAAGCCTGCATGCCAGACTGATCTGC
AAAAGTCGATTATATATTGTGGCAAGTCCAGACTATCTAAAAAAAGCTGGAACACCGAGCCATCCACAAGAACTGTTTCA
GCATCAATTGGTCGGGTTTAGCAAGCCTGTGTATTTAAATACATGGCCTTTAAAGATTGAAGATGAATACATCACCGTTC
ATCCTCAAATTAAGGCATCGAGTGGCGAAACAGTAAGGCAACTGACCCTGCGTGGTCATGGCATTGCACGTTTATCTGAG
TATGAAATCTGGCAGGATTTACAGGCTGGCAGTTTAATTGCCTTATTTGAAGACCAGATTGAAATTCAGTTGCAACATAT
TCATGCGGTGTATTATCAACAAAAGCATTTACCTAAACGTGTACGTTTATTTATTGATTTTTTAGCCGATGAACTTAGTC
AGCAATTTTCAACCACACAGGCGTTATAG

Upstream 100 bases:

>100_bases
TTATTCATCAGATGCTGAACATTTTGATCCGATTATTGTTGCGGAAAAACACATGAATGAGCAAAATATCTTTGCTTAAT
TGTCACGAATGGTATGACTT

Downstream 100 bases:

>100_bases
TGCTGTATTCGGAAGCTGAACCTTGAGTAACGGTGCTTTTTCATAAGGCCGTTGCAAACGAAAAACATTCTGATCCTGAC
TGCATTCGAATACCAACTGT

Product: transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MKSTVEELIAFITIVDTGSIVGAAEQLQQTPSGVSRSLNRLEKKLNVTLLERTTRKIKLTQEGSLFLMRARTILADLSDA
EEALLKSDSDTSGLIRIDSATPFVLHVLAPLMQKFMQLYPHIEIELNSNDQVIDLLQHKTDVAIRFGALHDSSLHARLIC
KSRLYIVASPDYLKKAGTPSHPQELFQHQLVGFSKPVYLNTWPLKIEDEYITVHPQIKASSGETVRQLTLRGHGIARLSE
YEIWQDLQAGSLIALFEDQIEIQLQHIHAVYYQQKHLPKRVRLFIDFLADELSQQFSTTQAL

Sequences:

>Translated_302_residues
MKSTVEELIAFITIVDTGSIVGAAEQLQQTPSGVSRSLNRLEKKLNVTLLERTTRKIKLTQEGSLFLMRARTILADLSDA
EEALLKSDSDTSGLIRIDSATPFVLHVLAPLMQKFMQLYPHIEIELNSNDQVIDLLQHKTDVAIRFGALHDSSLHARLIC
KSRLYIVASPDYLKKAGTPSHPQELFQHQLVGFSKPVYLNTWPLKIEDEYITVHPQIKASSGETVRQLTLRGHGIARLSE
YEIWQDLQAGSLIALFEDQIEIQLQHIHAVYYQQKHLPKRVRLFIDFLADELSQQFSTTQAL
>Mature_302_residues
MKSTVEELIAFITIVDTGSIVGAAEQLQQTPSGVSRSLNRLEKKLNVTLLERTTRKIKLTQEGSLFLMRARTILADLSDA
EEALLKSDSDTSGLIRIDSATPFVLHVLAPLMQKFMQLYPHIEIELNSNDQVIDLLQHKTDVAIRFGALHDSSLHARLIC
KSRLYIVASPDYLKKAGTPSHPQELFQHQLVGFSKPVYLNTWPLKIEDEYITVHPQIKASSGETVRQLTLRGHGIARLSE
YEIWQDLQAGSLIALFEDQIEIQLQHIHAVYYQQKHLPKRVRLFIDFLADELSQQFSTTQAL

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=291, Percent_Identity=43.6426116838488, Blast_Score=252, Evalue=2e-68,
Organism=Escherichia coli, GI145693193, Length=290, Percent_Identity=31.3793103448276, Blast_Score=137, Evalue=8e-34,
Organism=Escherichia coli, GI1789639, Length=292, Percent_Identity=30.8219178082192, Blast_Score=126, Evalue=1e-30,
Organism=Escherichia coli, GI1789440, Length=295, Percent_Identity=29.4915254237288, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI87081978, Length=297, Percent_Identity=27.2727272727273, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1787128, Length=293, Percent_Identity=25.5972696245734, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1787589, Length=292, Percent_Identity=26.3698630136986, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI87082132, Length=255, Percent_Identity=27.0588235294118, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1789173, Length=240, Percent_Identity=26.25, Blast_Score=81, Evalue=9e-17,
Organism=Escherichia coli, GI1788481, Length=300, Percent_Identity=26, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1786448, Length=298, Percent_Identity=24.8322147651007, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI145693105, Length=195, Percent_Identity=24.1025641025641, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI157672245, Length=162, Percent_Identity=30.2469135802469, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI1788706, Length=157, Percent_Identity=28.0254777070064, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1790783, Length=276, Percent_Identity=25, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI87082024, Length=158, Percent_Identity=29.1139240506329, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1788297, Length=165, Percent_Identity=29.0909090909091, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1790262, Length=184, Percent_Identity=25.5434782608696, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34219; Mature: 34219

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSTVEELIAFITIVDTGSIVGAAEQLQQTPSGVSRSLNRLEKKLNVTLLERTTRKIKLT
CCHHHHHHHHHHHEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHEECCCCEEEEE
QEGSLFLMRARTILADLSDAEEALLKSDSDTSGLIRIDSATPFVLHVLAPLMQKFMQLYP
CCCCEEEEEHHHHHHHCCHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCC
HIEIELNSNDQVIDLLQHKTDVAIRFGALHDSSLHARLICKSRLYIVASPDYLKKAGTPS
CEEEEECCCCHHHHHHHCCCCEEEEEECCCCCCCCEEEEEECCEEEEECCHHHHHCCCCC
HPQELFQHQLVGFSKPVYLNTWPLKIEDEYITVHPQIKASSGETVRQLTLRGHGIARLSE
CHHHHHHHHHHCCCCCEEEEECCEEEECCEEEEECEEECCCCCHHHHHHHCCCCCHHHHH
YEIWQDLQAGSLIALFEDQIEIQLQHIHAVYYQQKHLPKRVRLFIDFLADELSQQFSTTQ
HHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHC
AL
CC
>Mature Secondary Structure
MKSTVEELIAFITIVDTGSIVGAAEQLQQTPSGVSRSLNRLEKKLNVTLLERTTRKIKLT
CCHHHHHHHHHHHEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHEECCCCEEEEE
QEGSLFLMRARTILADLSDAEEALLKSDSDTSGLIRIDSATPFVLHVLAPLMQKFMQLYP
CCCCEEEEEHHHHHHHCCHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCC
HIEIELNSNDQVIDLLQHKTDVAIRFGALHDSSLHARLICKSRLYIVASPDYLKKAGTPS
CEEEEECCCCHHHHHHHCCCCEEEEEECCCCCCCCEEEEEECCEEEEECCHHHHHCCCCC
HPQELFQHQLVGFSKPVYLNTWPLKIEDEYITVHPQIKASSGETVRQLTLRGHGIARLSE
CHHHHHHHHHHCCCCCEEEEECCEEEECCEEEEECEEECCCCCHHHHHHHCCCCCHHHHH
YEIWQDLQAGSLIALFEDQIEIQLQHIHAVYYQQKHLPKRVRLFIDFLADELSQQFSTTQ
HHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHC
AL
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]