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The map label for this gene is glmU

Identifier: 50086528

GI number: 50086528

Start: 3496809

End: 3498173

Strand: Direct

Name: glmU

Synonym: ACIAD3575

Alternate gene names: 50086528

Gene position: 3496809-3498173 (Clockwise)

Preceding gene: 50086527

Following gene: 50086529

Centisome position: 97.17

GC content: 43.96

Gene sequence:

>1365_bases
ATGACAACGACCGTTATTATTCTTGCTGCTGGTAAAGGAACGCGCATGCGTTCCCATCTTCCTAAAGTTTTGCAACCACT
GGCAGGGCGTCCACTGCTTGGGCACGTTATCCAGACGGCAAAACAGCTCAATGCAAATAATATTATTACTATTTTTGGAC
ATGGTGGCGCACAGGTTCAGCAACAATTTCAGCAAGAAAATATTCAGTGGGTCGAACAAACCGAACAACTTGGTACAGGT
CATGCAGTACAAATGACCTTACCCGTATTACCACACGATGGTTTGTCTTTAATTCTTTATGGCGATGTTCCTTTAGTACG
TCAGCAAACCCTTGAAAAATTACTTGCGGTATCGAGCACCACAGGTATTGGCATGATTACGCTTCGTGTTGAAGATCCGA
CGGGCTATGGCCGAATTATCCGTGAAAGTGACAAAATTCAAGCGATTGTTGAACACAAAGATGCTTCTGAGCAACAGCGC
CAGATTCAGGAAATTAATACGGGCATCTATTGTGTAAGCAACCAAAAACTGCATGAATGGCTTCCAAAGCTTTCCAATAA
TAATGCACAAGGTGAATATTACCTCACCGACATCGTGGCAATGGCGGTGGCTGATGGACTTGAAATTGCGTCTATTCAAC
CCGATTTAGCTTTTGAAGTTGAAGGCGTCAATGACCGACTGCAACTGGCAGCGCTTGAACGAGAATTTCAGTTACAGCAA
GCCAAAAGCCTGATGCAGCAAGGCGTTACCCTGACTGACCCATCACGTTTTGATTTGCGTGGTACACTTAAAATTGGGCA
AGATGTCCGCATAGATATTAACGTCATTATCGAAGGCAACTGTGAGCTCGGTGATTTTGTTGAAATTGGTGCAGGTTGTG
TGCTCAAAAATACTAAAATTGCTGCTGGCACCAAAGTACAGCCTTACAGCATTTTTGAAGATGCTGTGGTCGGTGAAAAT
ACCCAGATTGGTCCATTTGCCCGCTTGCGCCCAGGTGCTCATCTGGCGGCCGAGGTACATATTGGCAATTTTGTTGAAGT
CAAAAACACAAGTATTGGTGTGGGTTCAAAAGCCAATCACTTTACTTATTTAGGTGATGCCGAAGTCGGTGCAGGTTCAA
ACATTGGTGCAGGTACCATTACCTGTAATTATGATGGCGCCAACAAGCATAAGACAGTTATTGGTGATGCAGTCTTTATC
GGTTCAAATAGCTCACTGGTTGCACCTGTAAGCATTGGTGATGGTGCAACAGTAGGTGCAGGTTCTGTCATTACACGTAA
TGTGCCAGAAAATACGTTGGCATTTGAGCGTGCGCAACAGATTGAAAAAGCGAATTATCAACGTCCACAAAAGTTGAAAA
AATAA

Upstream 100 bases:

>100_bases
GGCTTTGGCATCATGCTCGACGATATTATCGCTGGATTGTGGGCCGCACTGTGTATTTTGATATATTTATCTCTGGTCTA
AACCGTTAAGTAGGTTATTC

Downstream 100 bases:

>100_bases
GAGGAACTCAGTATGTGTGGTATCGTCGGAGGCGTTGCAGAACGTAATATTGCCGAGATTTTGATCGAGGGATTAAAACG
CCTTGAATACCGTGGATACG

Product: bifunctional UDP-N-acetylglucosamine pyrophosphorylase/glucosamine-1-phosphate N-acetyltransferase

Products: NA

Alternate protein names: UDP-N-acetylglucosamine pyrophosphorylase; N-acetylglucosamine-1-phosphate uridyltransferase; Glucosamine-1-phosphate N-acetyltransferase

Number of amino acids: Translated: 454; Mature: 453

Protein sequence:

>454_residues
MTTTVIILAAGKGTRMRSHLPKVLQPLAGRPLLGHVIQTAKQLNANNIITIFGHGGAQVQQQFQQENIQWVEQTEQLGTG
HAVQMTLPVLPHDGLSLILYGDVPLVRQQTLEKLLAVSSTTGIGMITLRVEDPTGYGRIIRESDKIQAIVEHKDASEQQR
QIQEINTGIYCVSNQKLHEWLPKLSNNNAQGEYYLTDIVAMAVADGLEIASIQPDLAFEVEGVNDRLQLAALEREFQLQQ
AKSLMQQGVTLTDPSRFDLRGTLKIGQDVRIDINVIIEGNCELGDFVEIGAGCVLKNTKIAAGTKVQPYSIFEDAVVGEN
TQIGPFARLRPGAHLAAEVHIGNFVEVKNTSIGVGSKANHFTYLGDAEVGAGSNIGAGTITCNYDGANKHKTVIGDAVFI
GSNSSLVAPVSIGDGATVGAGSVITRNVPENTLAFERAQQIEKANYQRPQKLKK

Sequences:

>Translated_454_residues
MTTTVIILAAGKGTRMRSHLPKVLQPLAGRPLLGHVIQTAKQLNANNIITIFGHGGAQVQQQFQQENIQWVEQTEQLGTG
HAVQMTLPVLPHDGLSLILYGDVPLVRQQTLEKLLAVSSTTGIGMITLRVEDPTGYGRIIRESDKIQAIVEHKDASEQQR
QIQEINTGIYCVSNQKLHEWLPKLSNNNAQGEYYLTDIVAMAVADGLEIASIQPDLAFEVEGVNDRLQLAALEREFQLQQ
AKSLMQQGVTLTDPSRFDLRGTLKIGQDVRIDINVIIEGNCELGDFVEIGAGCVLKNTKIAAGTKVQPYSIFEDAVVGEN
TQIGPFARLRPGAHLAAEVHIGNFVEVKNTSIGVGSKANHFTYLGDAEVGAGSNIGAGTITCNYDGANKHKTVIGDAVFI
GSNSSLVAPVSIGDGATVGAGSVITRNVPENTLAFERAQQIEKANYQRPQKLKK
>Mature_453_residues
TTTVIILAAGKGTRMRSHLPKVLQPLAGRPLLGHVIQTAKQLNANNIITIFGHGGAQVQQQFQQENIQWVEQTEQLGTGH
AVQMTLPVLPHDGLSLILYGDVPLVRQQTLEKLLAVSSTTGIGMITLRVEDPTGYGRIIRESDKIQAIVEHKDASEQQRQ
IQEINTGIYCVSNQKLHEWLPKLSNNNAQGEYYLTDIVAMAVADGLEIASIQPDLAFEVEGVNDRLQLAALEREFQLQQA
KSLMQQGVTLTDPSRFDLRGTLKIGQDVRIDINVIIEGNCELGDFVEIGAGCVLKNTKIAAGTKVQPYSIFEDAVVGENT
QIGPFARLRPGAHLAAEVHIGNFVEVKNTSIGVGSKANHFTYLGDAEVGAGSNIGAGTITCNYDGANKHKTVIGDAVFIG
SNSSLVAPVSIGDGATVGAGSVITRNVPENTLAFERAQQIEKANYQRPQKLKK

Specific function: Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-GlcNAc. Responsible for the acetylation of Glc-N-1-P to give GlcNAc-1-P and for the uridyl transfer from UTP to GlcNAc-1-P which produces UDP-GlcNAc

COG id: COG1207

COG function: function code M; N-acetylglucosamine-1-phosphate uridyltransferase (contains nucleotidyltransferase and I-patch acetyltransferase domains)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transferase hexapeptide repeat family

Homologues:

Organism=Escherichia coli, GI1790168, Length=450, Percent_Identity=56.4444444444444, Blast_Score=532, Evalue=1e-152,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLMU_ACIAD (Q6F6U9)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_048038.1
- ProteinModelPortal:   Q6F6U9
- SMR:   Q6F6U9
- STRING:   Q6F6U9
- GeneID:   2881139
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD3575
- NMPDR:   fig|62977.3.peg.3231
- eggNOG:   COG1207
- HOGENOM:   HBG688195
- OMA:   GSKVNHL
- PhylomeDB:   Q6F6U9
- ProtClustDB:   CLSK2307568
- BioCyc:   ASP62977:ACIAD3575-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01631
- InterPro:   IPR005882
- InterPro:   IPR018357
- InterPro:   IPR005835
- InterPro:   IPR011004
- TIGRFAMs:   TIGR01173

Pfam domain/function: PF00483 NTP_transferase; SSF51161 Trimer_LpxA_like

EC number: =2.7.7.23; =2.3.1.157

Molecular weight: Translated: 49024; Mature: 48892

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: ACT_SITE 360-360 BINDING 73-73 BINDING 137-137 BINDING 151-151 BINDING 166-166 BINDING 384-384 BINDING 402-402 BINDING 420-420 BINDING 437-437

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTTVIILAAGKGTRMRSHLPKVLQPLAGRPLLGHVIQTAKQLNANNIITIFGHGGAQVQ
CCEEEEEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEEEECCCHHHH
QQFQQENIQWVEQTEQLGTGHAVQMTLPVLPHDGLSLILYGDVPLVRQQTLEKLLAVSST
HHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCEEEEECCCCHHHHHHHHHHHHHCCC
TGIGMITLRVEDPTGYGRIIRESDKIQAIVEHKDASEQQRQIQEINTGIYCVSNQKLHEW
CCEEEEEEEEECCCCCCCEEECCCHHEEEECCCCCHHHHHHHHHHCCCEEEECCCHHHHH
LPKLSNNNAQGEYYLTDIVAMAVADGLEIASIQPDLAFEVEGVNDRLQLAALEREFQLQQ
HHHHCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHH
AKSLMQQGVTLTDPSRFDLRGTLKIGQDVRIDINVIIEGNCELGDFVEIGAGCVLKNTKI
HHHHHHCCCEECCCCCCCEEEEEEECCEEEEEEEEEEECCCCCCCHHHHCCCEEEECCEE
AAGTKVQPYSIFEDAVVGENTQIGPFARLRPGAHLAAEVHIGNFVEVKNTSIGVGSKANH
ECCCCCCCHHHHHHHHCCCCCCCCCHHHCCCCCCEEEEEECCCEEEEECCEECCCCCCCC
FTYLGDAEVGAGSNIGAGTITCNYDGANKHKTVIGDAVFIGSNSSLVAPVSIGDGATVGA
EEEECCCCCCCCCCCCCCEEEEECCCCCCCEEEEEEEEEECCCCCEEEEEECCCCCCCCC
GSVITRNVPENTLAFERAQQIEKANYQRPQKLKK
CCEEECCCCCHHHHHHHHHHHHHHCCCCCHHHCC
>Mature Secondary Structure 
TTTVIILAAGKGTRMRSHLPKVLQPLAGRPLLGHVIQTAKQLNANNIITIFGHGGAQVQ
CEEEEEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEEEECCCHHHH
QQFQQENIQWVEQTEQLGTGHAVQMTLPVLPHDGLSLILYGDVPLVRQQTLEKLLAVSST
HHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCEEEEECCCCHHHHHHHHHHHHHCCC
TGIGMITLRVEDPTGYGRIIRESDKIQAIVEHKDASEQQRQIQEINTGIYCVSNQKLHEW
CCEEEEEEEEECCCCCCCEEECCCHHEEEECCCCCHHHHHHHHHHCCCEEEECCCHHHHH
LPKLSNNNAQGEYYLTDIVAMAVADGLEIASIQPDLAFEVEGVNDRLQLAALEREFQLQQ
HHHHCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHH
AKSLMQQGVTLTDPSRFDLRGTLKIGQDVRIDINVIIEGNCELGDFVEIGAGCVLKNTKI
HHHHHHCCCEECCCCCCCEEEEEEECCEEEEEEEEEEECCCCCCCHHHHCCCEEEECCEE
AAGTKVQPYSIFEDAVVGENTQIGPFARLRPGAHLAAEVHIGNFVEVKNTSIGVGSKANH
ECCCCCCCHHHHHHHHCCCCCCCCCHHHCCCCCCEEEEEECCCEEEEECCEECCCCCCCC
FTYLGDAEVGAGSNIGAGTITCNYDGANKHKTVIGDAVFIGSNSSLVAPVSIGDGATVGA
EEEECCCCCCCCCCCCCCEEEEECCCCCCCEEEEEEEEEECCCCCEEEEEECCCCCCCCC
GSVITRNVPENTLAFERAQQIEKANYQRPQKLKK
CCEEECCCCCHHHHHHHHHHHHHHCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA