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The map label for this gene is nagX [H]

Identifier: 50086495

GI number: 50086495

Start: 3465147

End: 3466304

Strand: Reverse

Name: nagX [H]

Synonym: ACIAD3540

Alternate gene names: 50086495

Gene position: 3466304-3465147 (Counterclockwise)

Preceding gene: 50086499

Following gene: 50086490

Centisome position: 96.32

GC content: 43.78

Gene sequence:

>1158_bases
ATGAATGTGGTCATTATTGGTGCAGGTATGGGCGGCTTAACCACAGGAATTGCCTTAAAAAAATTTGGTCATCAAGTTAC
AATTTTTGAACAAGCTGAACAAATTCTTCCTGTTGGTGCTGCAATTTCATTGTGGTCCAATGGTGTTAAATGCTTGAACT
ATCTTGGATTGAATGAACAAATTGCCAAACTCGGCGGTCAGATGGACAACCTCGCTTATGTTGATGGTTTGACTGGCGAT
GTAATGACTGAATTTAGTCTTCAGCCCTTAATTGAGGAAGTCGGGCAGCGTCCTTATCCTGTGTCACGTGCCGAATTGCA
AAATATGTTAATGGATGAGTTTGGTCGTGAAGATATTCATTTGGGTAAACGTATGGTGGCACTGCAACAAAAGGATGATC
AGGTTGAAATTGAATTTGCAGATGGCAGTTCAATTCTGGCTGATGTACTGGTTGGAGCAGATGGTACACATTCGATTACC
CGTACTTATGTGCTGGGTGAGAAAGTTGAGCGTCGCTATGCGGGTTACGTCAACTGGAATGGTCTGGTTGATATTTCTTC
AGATTTGGCGCCTGCCGATCAATGGACCACTTATGTAGGTGAAGGCAAACGTGCTTCGCTTATGCCTGTAGCAGATAATC
GTTTTTATTTCTTTCTGGATGTGCCTTTGGAAGCAGGTCTGGAAAATGATAAATGTAAATATAAAGAAACACTGCAATCT
TATTTTAAGGGTTGGTGCCCGCAAGTACAGACCTTGATTGAGCGTCTTGATCCTCAAAAAACCAATCGAGTCGAAATTTG
CGATATTGAGCCTTTTGCCCAGTTCTATAAAGGTCGCGTGGTGTTGGTGGGCGATGCAGCACATAGCACAACACCAGATA
TTGGTCAGGGTGGCTGTCAGGCCATGGAAGATGCCATCTATCTGGCACGTTCTTTACAGATCAATACACTCAGTGTGGAG
GATGCACTCAGACGTTATCAGGAAAAGCGCAATCAACGTGCCAATGAACTGGTTTTGCGTGCCAGAAAGCGTTGTGATGT
GACCCATATGAAGGATGAAGCCGTGACTACAGCATGGTATGCCGAATTACGTCAGGAGAAAGGCTTGCATATCATGAACG
GCATTATCAGTAATATTGTAGGCAACCCGCTCGATTAA

Upstream 100 bases:

>100_bases
AGGGTATATGCCAATTCCAATAATCCTGTCAATTTCCCTACACGTGGCATGGATTTAGCTTAATTAATTATAAAATTGTA
GTAAAAATAAGGAGTAAGAC

Downstream 100 bases:

>100_bases
TAGTTGATTAAAAAAATCAAGGTCAATCCAGACCTTGATTCTGATCAGTTTAGAAATGGTATTTGATGAGTGCGCTGACA
TTACGCTCATCTCGCCCTTC

Product: flavoprotein monooxygenase acting on aromatic compound

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 385; Mature: 385

Protein sequence:

>385_residues
MNVVIIGAGMGGLTTGIALKKFGHQVTIFEQAEQILPVGAAISLWSNGVKCLNYLGLNEQIAKLGGQMDNLAYVDGLTGD
VMTEFSLQPLIEEVGQRPYPVSRAELQNMLMDEFGREDIHLGKRMVALQQKDDQVEIEFADGSSILADVLVGADGTHSIT
RTYVLGEKVERRYAGYVNWNGLVDISSDLAPADQWTTYVGEGKRASLMPVADNRFYFFLDVPLEAGLENDKCKYKETLQS
YFKGWCPQVQTLIERLDPQKTNRVEICDIEPFAQFYKGRVVLVGDAAHSTTPDIGQGGCQAMEDAIYLARSLQINTLSVE
DALRRYQEKRNQRANELVLRARKRCDVTHMKDEAVTTAWYAELRQEKGLHIMNGIISNIVGNPLD

Sequences:

>Translated_385_residues
MNVVIIGAGMGGLTTGIALKKFGHQVTIFEQAEQILPVGAAISLWSNGVKCLNYLGLNEQIAKLGGQMDNLAYVDGLTGD
VMTEFSLQPLIEEVGQRPYPVSRAELQNMLMDEFGREDIHLGKRMVALQQKDDQVEIEFADGSSILADVLVGADGTHSIT
RTYVLGEKVERRYAGYVNWNGLVDISSDLAPADQWTTYVGEGKRASLMPVADNRFYFFLDVPLEAGLENDKCKYKETLQS
YFKGWCPQVQTLIERLDPQKTNRVEICDIEPFAQFYKGRVVLVGDAAHSTTPDIGQGGCQAMEDAIYLARSLQINTLSVE
DALRRYQEKRNQRANELVLRARKRCDVTHMKDEAVTTAWYAELRQEKGLHIMNGIISNIVGNPLD
>Mature_385_residues
MNVVIIGAGMGGLTTGIALKKFGHQVTIFEQAEQILPVGAAISLWSNGVKCLNYLGLNEQIAKLGGQMDNLAYVDGLTGD
VMTEFSLQPLIEEVGQRPYPVSRAELQNMLMDEFGREDIHLGKRMVALQQKDDQVEIEFADGSSILADVLVGADGTHSIT
RTYVLGEKVERRYAGYVNWNGLVDISSDLAPADQWTTYVGEGKRASLMPVADNRFYFFLDVPLEAGLENDKCKYKETLQS
YFKGWCPQVQTLIERLDPQKTNRVEICDIEPFAQFYKGRVVLVGDAAHSTTPDIGQGGCQAMEDAIYLARSLQINTLSVE
DALRRYQEKRNQRANELVLRARKRCDVTHMKDEAVTTAWYAELRQEKGLHIMNGIISNIVGNPLD

Specific function: Catalyzes the NAD- or NADP-dependent conversion of 3- hydroxybenzoate to gentisate. The affinity of the enzyme toward NAD is twice as high as for NADP [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxybenzoate 6-hydroxylase family [H]

Homologues:

Organism=Escherichia coli, GI1789274, Length=357, Percent_Identity=23.8095238095238, Blast_Score=73, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: =1.14.13.24 [H]

Molecular weight: Translated: 42916; Mature: 42916

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVVIIGAGMGGLTTGIALKKFGHQVTIFEQAEQILPVGAAISLWSNGVKCLNYLGLNEQ
CEEEEEECCCCHHHHHHHHHHHCCEEEEHHHHHHHCCCHHHHHHHHCCHHHHHHCCCCHH
IAKLGGQMDNLAYVDGLTGDVMTEFSLQPLIEEVGQRPYPVSRAELQNMLMDEFGREDIH
HHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCHHHH
LGKRMVALQQKDDQVEIEFADGSSILADVLVGADGTHSITRTYVLGEKVERRYAGYVNWN
HHHHHHHHCCCCCEEEEEECCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCEECCC
GLVDISSDLAPADQWTTYVGEGKRASLMPVADNRFYFFLDVPLEAGLENDKCKYKETLQS
CEEEECCCCCCCHHHHHHHCCCCCCEEEEEECCCEEEEEECCHHCCCCCCCHHHHHHHHH
YFKGWCPQVQTLIERLDPQKTNRVEICDIEPFAQFYKGRVVLVGDAAHSTTPDIGQGGCQ
HHHCCCHHHHHHHHHCCCCCCCCEEEECCCHHHHHHCCCEEEEECCCCCCCCCCCCCHHH
AMEDAIYLARSLQINTLSVEDALRRYQEKRNQRANELVLRARKRCDVTHMKDEAVTTAWY
HHHHHHHHHHHCEEEEECHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCHHHHHHHHH
AELRQEKGLHIMNGIISNIVGNPLD
HHHHHHCCHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MNVVIIGAGMGGLTTGIALKKFGHQVTIFEQAEQILPVGAAISLWSNGVKCLNYLGLNEQ
CEEEEEECCCCHHHHHHHHHHHCCEEEEHHHHHHHCCCHHHHHHHHCCHHHHHHCCCCHH
IAKLGGQMDNLAYVDGLTGDVMTEFSLQPLIEEVGQRPYPVSRAELQNMLMDEFGREDIH
HHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCHHHH
LGKRMVALQQKDDQVEIEFADGSSILADVLVGADGTHSITRTYVLGEKVERRYAGYVNWN
HHHHHHHHCCCCCEEEEEECCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCEECCC
GLVDISSDLAPADQWTTYVGEGKRASLMPVADNRFYFFLDVPLEAGLENDKCKYKETLQS
CEEEECCCCCCCHHHHHHHCCCCCCEEEEEECCCEEEEEECCHHCCCCCCCHHHHHHHHH
YFKGWCPQVQTLIERLDPQKTNRVEICDIEPFAQFYKGRVVLVGDAAHSTTPDIGQGGCQ
HHHCCCHHHHHHHHHCCCCCCCCEEEECCCHHHHHHCCCEEEEECCCCCCCCCCCCCHHH
AMEDAIYLARSLQINTLSVEDALRRYQEKRNQRANELVLRARKRCDVTHMKDEAVTTAWY
HHHHHHHHHHHCEEEEECHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCHHHHHHHHH
AELRQEKGLHIMNGIISNIVGNPLD
HHHHHHCCHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA