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The map label for this gene is est [H]

Identifier: 50086485

GI number: 50086485

Start: 3456055

End: 3456960

Strand: Direct

Name: est [H]

Synonym: ACIAD3530

Alternate gene names: 50086485

Gene position: 3456055-3456960 (Clockwise)

Preceding gene: 50086483

Following gene: 50086486

Centisome position: 96.04

GC content: 42.6

Gene sequence:

>906_bases
TTGTATATTATGTGGAAACAATATTTAACAGAAGCTGTTTTAAAAACCACCATTCGAGCCCCAAGCCGGTTTAGCCTTCC
ACCCGCAACCCTTCGTGTGGCCCTGAATCAGATGTGCCGAGTTTTTCCGCTCAATAAAGAAGTGAGTATTCGCCCAATTA
GGCTGGCAGGGATACGTGCGGAAGAAATTAAACCTCAGTCGTCATCGACTCAACTGATTTTTCATATCCATGGCGGGGCT
TTTTTTCTTGGCAGTTTAAATACGCATCGTGCATTGATGACAGAGCTGGCAGCACGGACACAAATGCAAGTTTTACATGT
GGATTATCCATTGGCTCCCGAAGCAGGATTTCCAGATGCTTTAGATGCGCTTTACGATATTTACCAAACTTTGCTTGATC
AGGGTGTACTTCCCAAAGACATTATCTTGTCAGGTGATTCCTGTGGCGCAAATCTGGCCCTCGCGTTGGCATTACGTCTG
AAAGATGATCCAGAACAGCTACCAAGTGGCCTGATTTTAATGTCACCTTTTTTAGATCTGACATTAAGCAGTGAATCATT
ACGACTCAACAAAAAACACGATGCCTTGCTTTGTATAGAAGCACTTGAAGCTGGCATAGATTACTATCTTGGTGACAGTA
TGCCAAAAGATGATCCGCGTGTTTCGCCTATTTTTGACAATTTACGTGGGCTCCCTCCGACAATGATTCAAGTTGGATCC
AAAGAAATTTTACTGGACGATGCCAAACGCTTTCGAGATAAAGCCAAAGAAGCAGATGTAAAAGTTGAATTTAAGCTATA
TACAGGGATGTGGCATAATTTTCAGATGTTTAGTGCATGGTTTGATGAGGCCAAACAGGCCATTTCAGATCTCGCAGTAT
TTGCTCATCAACTTGATCGTGATTAA

Upstream 100 bases:

>100_bases
CAATTTTTGTGTGTGACTATTTAGTGTAGCCTTGTAAAGTCTGAGGATAAATACACACGCATTGCGAATAAACTGTAAGA
ATCCGCAACAATAATAAAGG

Downstream 100 bases:

>100_bases
ATGCAAGCTGGCATTTTGCCAGCGACATTTAAAAAAAAATAAGAAGAAGAAATATCAGATAGATCATAATAAATGCTATC
TTAAACGCTGAAATCTAATA

Product: esterase precursor

Products: alcohol; a carboxylate [C]

Alternate protein names: NA

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MYIMWKQYLTEAVLKTTIRAPSRFSLPPATLRVALNQMCRVFPLNKEVSIRPIRLAGIRAEEIKPQSSSTQLIFHIHGGA
FFLGSLNTHRALMTELAARTQMQVLHVDYPLAPEAGFPDALDALYDIYQTLLDQGVLPKDIILSGDSCGANLALALALRL
KDDPEQLPSGLILMSPFLDLTLSSESLRLNKKHDALLCIEALEAGIDYYLGDSMPKDDPRVSPIFDNLRGLPPTMIQVGS
KEILLDDAKRFRDKAKEADVKVEFKLYTGMWHNFQMFSAWFDEAKQAISDLAVFAHQLDRD

Sequences:

>Translated_301_residues
MYIMWKQYLTEAVLKTTIRAPSRFSLPPATLRVALNQMCRVFPLNKEVSIRPIRLAGIRAEEIKPQSSSTQLIFHIHGGA
FFLGSLNTHRALMTELAARTQMQVLHVDYPLAPEAGFPDALDALYDIYQTLLDQGVLPKDIILSGDSCGANLALALALRL
KDDPEQLPSGLILMSPFLDLTLSSESLRLNKKHDALLCIEALEAGIDYYLGDSMPKDDPRVSPIFDNLRGLPPTMIQVGS
KEILLDDAKRFRDKAKEADVKVEFKLYTGMWHNFQMFSAWFDEAKQAISDLAVFAHQLDRD
>Mature_301_residues
MYIMWKQYLTEAVLKTTIRAPSRFSLPPATLRVALNQMCRVFPLNKEVSIRPIRLAGIRAEEIKPQSSSTQLIFHIHGGA
FFLGSLNTHRALMTELAARTQMQVLHVDYPLAPEAGFPDALDALYDIYQTLLDQGVLPKDIILSGDSCGANLALALALRL
KDDPEQLPSGLILMSPFLDLTLSSESLRLNKKHDALLCIEALEAGIDYYLGDSMPKDDPRVSPIFDNLRGLPPTMIQVGS
KEILLDDAKRFRDKAKEADVKVEFKLYTGMWHNFQMFSAWFDEAKQAISDLAVFAHQLDRD

Specific function: Has An Esterase Activity. Triacetyl Glycerol (Triacetin) Is A Substrate Of The Enzyme. [C]

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Secreted (Potential). Note=External to the cytoplasmic membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI21328446, Length=167, Percent_Identity=31.1377245508982, Blast_Score=74, Evalue=1e-13,
Organism=Escherichia coli, GI1786682, Length=252, Percent_Identity=32.1428571428571, Blast_Score=104, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI17567059, Length=307, Percent_Identity=26.3843648208469, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24656084, Length=114, Percent_Identity=37.719298245614, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24656076, Length=114, Percent_Identity=37.719298245614, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI20130169, Length=114, Percent_Identity=37.719298245614, Blast_Score=81, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.- [C]

Molecular weight: Translated: 33790; Mature: 33790

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: PS01173 LIPASE_GDXG_HIS ; PS01174 LIPASE_GDXG_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYIMWKQYLTEAVLKTTIRAPSRFSLPPATLRVALNQMCRVFPLNKEVSIRPIRLAGIRA
CEEEHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCH
EEIKPQSSSTQLIFHIHGGAFFLGSLNTHRALMTELAARTQMQVLHVDYPLAPEAGFPDA
HHCCCCCCCCEEEEEEECCEEEEECCCHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCHH
LDALYDIYQTLLDQGVLPKDIILSGDSCGANLALALALRLKDDPEQLPSGLILMSPFLDL
HHHHHHHHHHHHHCCCCCHHEEEECCCCCCCEEEEEEECCCCCHHHCCCCEEEECCHHHE
TLSSESLRLNKKHDALLCIEALEAGIDYYLGDSMPKDDPRVSPIFDNLRGLPPTMIQVGS
EECCCCEEECCCCCHHHHHHHHHCCCCEEECCCCCCCCCCCCHHHHHHCCCCHHHHHCCC
KEILLDDAKRFRDKAKEADVKVEFKLYTGMWHNFQMFSAWFDEAKQAISDLAVFAHQLDR
CHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
D
C
>Mature Secondary Structure
MYIMWKQYLTEAVLKTTIRAPSRFSLPPATLRVALNQMCRVFPLNKEVSIRPIRLAGIRA
CEEEHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCH
EEIKPQSSSTQLIFHIHGGAFFLGSLNTHRALMTELAARTQMQVLHVDYPLAPEAGFPDA
HHCCCCCCCCEEEEEEECCEEEEECCCHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCHH
LDALYDIYQTLLDQGVLPKDIILSGDSCGANLALALALRLKDDPEQLPSGLILMSPFLDL
HHHHHHHHHHHHHCCCCCHHEEEECCCCCCCEEEEEEECCCCCHHHCCCCEEEECCHHHE
TLSSESLRLNKKHDALLCIEALEAGIDYYLGDSMPKDDPRVSPIFDNLRGLPPTMIQVGS
EECCCCEEECCCCCHHHHHHHHHCCCCEEECCCCCCCCCCCCHHHHHHCCCCHHHHHCCC
KEILLDDAKRFRDKAKEADVKVEFKLYTGMWHNFQMFSAWFDEAKQAISDLAVFAHQLDR
CHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
D
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Carboxylic ester; H2O [C]

Specific reaction: Carboxylic ester + H2O = an alcohol + carboxylate [C]

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2545531 [H]