Click here to switch to the map view.

The map label for this gene is mraW

Identifier: 50086346

GI number: 50086346

Start: 3277338

End: 3278249

Strand: Reverse

Name: mraW

Synonym: ACIAD3368

Alternate gene names: 50086346

Gene position: 3278249-3277338 (Counterclockwise)

Preceding gene: 50086347

Following gene: 50086345

Centisome position: 91.1

GC content: 41.78

Gene sequence:

>912_bases
ATGTCGCATATTTCTGTCTTACTTCACGAAACTGTAGATGCCTTATTGGCCAACCGCGACACAGGAATTTATATTGACGG
CACTTTTGGCCGTGGTGGACATACTCGCTTATTACTTTCAAAACTTGGATCAGACGCGCAAGTATACGGTTTTGATAAAG
ACCCTCAAGCATTGGCTGTTGCTCATCAATTAGAACAAGAAGATCTACGGTTTAAAATTATTCATGCCAGTTTTGCCGAT
ATACAAACAGAACTTGAACAGCGCGGTATTACACAGGTCGAAGGTGTCATGGCTGATTTGGGGGTCTCTTCTCCGCAGTT
GGATCAGGCAGAGCGTGGTTTTAGTTTTATGCAAGATGGTCCACTCGATATGCGTATGGATAATTCACAGGGAAAAACTG
CCGCAGAATGGTTATTAAGTATTGAAGAAGAAGCATTAGCAAATATTATTTATCAGTATGGTGAAGAGCGTTATAGCCGA
CGTATTGCCCGAGCAATCAAACAGGCAGGTGCACTTGAAACCACAGCACAACTTGCTGAGTTGGTAAAAACTGCTCATCC
AAAATGGGAAAAGCACAAACATCCTGCAACACGTACATTTCAGGCGATACGCATTGCGATTAATAAAGAGCTTGAAGATA
TTGAAATTTTCTTGCCTCAGGCGGTCACATTATTAAAGCCAGAAGGTCGTCTCGCAGTAATTACTTTCCACTCGCTTGAA
GATCGTTTAATTAAGCAGTTTATTCAAAAAGAATCGACTTTACCTGAAGATTCTGGCTGGGGACTACCTCAAAAACAAGT
TGACTCAAGACGTTTAAAGAAAATTTCCCGTATACGGGCCAGTGAAGAAGAAGTAAAAGCTAATCCTCGTTCACGTAGTG
CATGGCTTCGTGTGGCTGAACGTTTAGTATAA

Upstream 100 bases:

>100_bases
GATGAAAAGTAATATTTCCCTACAAAAAGACGTTTGGAGCTTTATTAAAACAAGGTAATAATGTGCAGTTAAATTTTTAA
CATTTTTTGACGCCTTACCT

Downstream 100 bases:

>100_bases
AGGCAAATCATGGACAAGAACGAAAAACTTGGAATATCTGAACCGTCACCTGTAAAAAGAGTTGTGGTGTATACAGTGAT
GGTTTTACTGGTATTTTGTA

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 303; Mature: 302

Protein sequence:

>303_residues
MSHISVLLHETVDALLANRDTGIYIDGTFGRGGHTRLLLSKLGSDAQVYGFDKDPQALAVAHQLEQEDLRFKIIHASFAD
IQTELEQRGITQVEGVMADLGVSSPQLDQAERGFSFMQDGPLDMRMDNSQGKTAAEWLLSIEEEALANIIYQYGEERYSR
RIARAIKQAGALETTAQLAELVKTAHPKWEKHKHPATRTFQAIRIAINKELEDIEIFLPQAVTLLKPEGRLAVITFHSLE
DRLIKQFIQKESTLPEDSGWGLPQKQVDSRRLKKISRIRASEEEVKANPRSRSAWLRVAERLV

Sequences:

>Translated_303_residues
MSHISVLLHETVDALLANRDTGIYIDGTFGRGGHTRLLLSKLGSDAQVYGFDKDPQALAVAHQLEQEDLRFKIIHASFAD
IQTELEQRGITQVEGVMADLGVSSPQLDQAERGFSFMQDGPLDMRMDNSQGKTAAEWLLSIEEEALANIIYQYGEERYSR
RIARAIKQAGALETTAQLAELVKTAHPKWEKHKHPATRTFQAIRIAINKELEDIEIFLPQAVTLLKPEGRLAVITFHSLE
DRLIKQFIQKESTLPEDSGWGLPQKQVDSRRLKKISRIRASEEEVKANPRSRSAWLRVAERLV
>Mature_302_residues
SHISVLLHETVDALLANRDTGIYIDGTFGRGGHTRLLLSKLGSDAQVYGFDKDPQALAVAHQLEQEDLRFKIIHASFADI
QTELEQRGITQVEGVMADLGVSSPQLDQAERGFSFMQDGPLDMRMDNSQGKTAAEWLLSIEEEALANIIYQYGEERYSRR
IARAIKQAGALETTAQLAELVKTAHPKWEKHKHPATRTFQAIRIAINKELEDIEIFLPQAVTLLKPEGRLAVITFHSLED
RLIKQFIQKESTLPEDSGWGLPQKQVDSRRLKKISRIRASEEEVKANPRSRSAWLRVAERLV

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=339, Percent_Identity=36.8731563421829, Blast_Score=185, Evalue=6e-47,
Organism=Homo sapiens, GI165377202, Length=189, Percent_Identity=40.7407407407407, Blast_Score=124, Evalue=1e-28,
Organism=Escherichia coli, GI1786270, Length=308, Percent_Identity=50.6493506493506, Blast_Score=301, Evalue=5e-83,
Organism=Drosophila melanogaster, GI62472493, Length=332, Percent_Identity=38.5542168674699, Blast_Score=186, Evalue=2e-47,
Organism=Drosophila melanogaster, GI28571637, Length=332, Percent_Identity=38.5542168674699, Blast_Score=186, Evalue=2e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_ACIAD (Q6F7D1)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_047856.1
- ProteinModelPortal:   Q6F7D1
- SMR:   Q6F7D1
- STRING:   Q6F7D1
- GeneID:   2878500
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD3368
- NMPDR:   fig|62977.3.peg.3049
- eggNOG:   COG0275
- HOGENOM:   HBG302779
- OMA:   PQLDDPE
- PhylomeDB:   Q6F7D1
- ProtClustDB:   PRK00050
- BioCyc:   ASP62977:ACIAD3368-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 34201; Mature: 34070

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: NA

Important sites: BINDING 52-52 BINDING 78-78 BINDING 99-99 BINDING 106-106

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHISVLLHETVDALLANRDTGIYIDGTFGRGGHTRLLLSKLGSDAQVYGFDKDPQALAV
CCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHCCCCCEEECCCCCHHHHHH
AHQLEQEDLRFKIIHASFADIQTELEQRGITQVEGVMADLGVSSPQLDQAERGFSFMQDG
HHHHHHHCCEEEEEEHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHCCCHHHCCC
PLDMRMDNSQGKTAAEWLLSIEEEALANIIYQYGEERYSRRIARAIKQAGALETTAQLAE
CCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
LVKTAHPKWEKHKHPATRTFQAIRIAINKELEDIEIFLPQAVTLLKPEGRLAVITFHSLE
HHHHCCCCHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHEECCCCCEEEEEEHHHH
DRLIKQFIQKESTLPEDSGWGLPQKQVDSRRLKKISRIRASEEEVKANPRSRSAWLRVAE
HHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHH
RLV
HCC
>Mature Secondary Structure 
SHISVLLHETVDALLANRDTGIYIDGTFGRGGHTRLLLSKLGSDAQVYGFDKDPQALAV
CHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHCCCCCEEECCCCCHHHHHH
AHQLEQEDLRFKIIHASFADIQTELEQRGITQVEGVMADLGVSSPQLDQAERGFSFMQDG
HHHHHHHCCEEEEEEHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHCCCHHHCCC
PLDMRMDNSQGKTAAEWLLSIEEEALANIIYQYGEERYSRRIARAIKQAGALETTAQLAE
CCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
LVKTAHPKWEKHKHPATRTFQAIRIAINKELEDIEIFLPQAVTLLKPEGRLAVITFHSLE
HHHHCCCCHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHEECCCCCEEEEEEHHHH
DRLIKQFIQKESTLPEDSGWGLPQKQVDSRRLKKISRIRASEEEVKANPRSRSAWLRVAE
HHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHH
RLV
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA