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The map label for this gene is sbp [H]

Identifier: 50086347

GI number: 50086347

Start: 3278627

End: 3279628

Strand: Reverse

Name: sbp [H]

Synonym: ACIAD3369

Alternate gene names: 50086347

Gene position: 3279628-3278627 (Counterclockwise)

Preceding gene: 50086351

Following gene: 50086346

Centisome position: 91.14

GC content: 42.12

Gene sequence:

>1002_bases
GTGAAAAAAATATGGGGTGTAACGTTTGCGATCTTGCTAGGCTTTGGAGCGGGGACTGCCAACGCAGCGAAAGATTTTTT
AAATGTGTCCTATGATCCGACACGTGAATTTTATCAAGAATATAATCAGAGTTTTTACACTTACTGGAAAGGCCGGACAG
GTCAGGAAGTCGATTTTAAGCAATCTCATGGCGGCTCGGGTAAGCAAGCTCGTGCGGTTATTGATGGTTTACAAGCGGAC
GTTGTGACATTAGCACTTGCCAATGATATTGACGAAATTGCCAACGCAGGTTTAATCAAAAAGAACTGGCAAAAGGATTT
TCCCAATAATTCGGCTCCCTACACTTCAACTATTGTATTTCTGGTACGAAAAGGTAATCCCAAAAATATTAAAGATTGGA
ATGATCTGACCAAGCCGGGTGTCGAAATTATTACACCAAACCCGAAAACTGGCGGAGCACCAAGATGGATTTATTTGGCG
GCATGGGGATATGCATTGAAGCAGCCAGGTGGCAACGACAGCAAAGCCAGAGAGCTGGTTAAAAAGCTTTATCATAACGT
GAAAGTTCTTGATTCTGGTGCAAGAGGATCGCTGACCACCTTTGCAGAACGTGGTATAGGCGATGTGCTGTTATCGTGGG
AAAACGAAGCATTGTTGGCAACACAAGGTCCGGGCAAAGATAAATATGAAATTATTTATCCTTCGATTTCAATTTTGGCA
GAACCTTCCGTTGCAATTGTGGATAAAACCGTTGACCGCGATGGAAATAGAAATCTAGCCAAAGCTTATTTAAACTATTT
GTATTCGCCACTTGGACAAGAATTGGCTGCCAAATACAATTTTAGACCTCGTAATGCAAAGGCGGCTGCCAAATACGCGT
ATAAGTTTCCAAAGCTTAAACTGTTCACCATTGGAGATGTCTTTGGTGGCTGGGCCAAAGCACAAAAAACGCATTTTGTG
AATGGTGCGATTTTTGATCAAATTTATGCGGAAAAACCATAA

Upstream 100 bases:

>100_bases
AAAACAGATTTGCAATGTTGTTATTGATATAACTATATGATTGTAAATTTATTTATTTTTTATATATTTAAAATAGGGTA
CATAATTTGAGCATATATCA

Downstream 100 bases:

>100_bases
TTTAAATTGAATCCATTAAACACGAATTACAACTTTGCGATTCGTGTTTTTCTTTCATGATGAAATCTAAATGAGCTTTA
TATGTTTAAAGCTGACTTTG

Product: sulfate ABC transporter periplasmic substrate-binding protein

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: Sulfate starvation-induced protein 2; SSI2 [H]

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MKKIWGVTFAILLGFGAGTANAAKDFLNVSYDPTREFYQEYNQSFYTYWKGRTGQEVDFKQSHGGSGKQARAVIDGLQAD
VVTLALANDIDEIANAGLIKKNWQKDFPNNSAPYTSTIVFLVRKGNPKNIKDWNDLTKPGVEIITPNPKTGGAPRWIYLA
AWGYALKQPGGNDSKARELVKKLYHNVKVLDSGARGSLTTFAERGIGDVLLSWENEALLATQGPGKDKYEIIYPSISILA
EPSVAIVDKTVDRDGNRNLAKAYLNYLYSPLGQELAAKYNFRPRNAKAAAKYAYKFPKLKLFTIGDVFGGWAKAQKTHFV
NGAIFDQIYAEKP

Sequences:

>Translated_333_residues
MKKIWGVTFAILLGFGAGTANAAKDFLNVSYDPTREFYQEYNQSFYTYWKGRTGQEVDFKQSHGGSGKQARAVIDGLQAD
VVTLALANDIDEIANAGLIKKNWQKDFPNNSAPYTSTIVFLVRKGNPKNIKDWNDLTKPGVEIITPNPKTGGAPRWIYLA
AWGYALKQPGGNDSKARELVKKLYHNVKVLDSGARGSLTTFAERGIGDVLLSWENEALLATQGPGKDKYEIIYPSISILA
EPSVAIVDKTVDRDGNRNLAKAYLNYLYSPLGQELAAKYNFRPRNAKAAAKYAYKFPKLKLFTIGDVFGGWAKAQKTHFV
NGAIFDQIYAEKP
>Mature_333_residues
MKKIWGVTFAILLGFGAGTANAAKDFLNVSYDPTREFYQEYNQSFYTYWKGRTGQEVDFKQSHGGSGKQARAVIDGLQAD
VVTLALANDIDEIANAGLIKKNWQKDFPNNSAPYTSTIVFLVRKGNPKNIKDWNDLTKPGVEIITPNPKTGGAPRWIYLA
AWGYALKQPGGNDSKARELVKKLYHNVKVLDSGARGSLTTFAERGIGDVLLSWENEALLATQGPGKDKYEIIYPSISILA
EPSVAIVDKTVDRDGNRNLAKAYLNYLYSPLGQELAAKYNFRPRNAKAAAKYAYKFPKLKLFTIGDVFGGWAKAQKTHFV
NGAIFDQIYAEKP

Specific function: This protein specifically binds sulfate and is involved in its transmembrane transport [H]

COG id: COG1613

COG function: function code P; ABC-type sulfate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic sulfate-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1790351, Length=326, Percent_Identity=64.1104294478528, Blast_Score=426, Evalue=1e-121,
Organism=Escherichia coli, GI1788765, Length=310, Percent_Identity=46.4516129032258, Blast_Score=277, Evalue=7e-76,

Paralogues:

None

Copy number: 1240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR000957
- InterPro:   IPR005669 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 36906; Mature: 36906

Theoretical pI: Translated: 9.93; Mature: 9.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
0.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
0.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIWGVTFAILLGFGAGTANAAKDFLNVSYDPTREFYQEYNQSFYTYWKGRTGQEVDFK
CCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHCCCEEEEECCCCCCCCCCH
QSHGGSGKQARAVIDGLQADVVTLALANDIDEIANAGLIKKNWQKDFPNNSAPYTSTIVF
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCCCCCCEEEEEE
LVRKGNPKNIKDWNDLTKPGVEIITPNPKTGGAPRWIYLAAWGYALKQPGGNDSKARELV
EEECCCCCCCCCCHHHCCCCCEEECCCCCCCCCCCEEEEEECCHHHCCCCCCHHHHHHHH
KKLYHNVKVLDSGARGSLTTFAERGIGDVLLSWENEALLATQGPGKDKYEIIYPSISILA
HHHHCCEEEEECCCCCCHHHHHHCCCCCEEEECCCCEEEEECCCCCCCEEEECCCCEEEE
EPSVAIVDKTVDRDGNRNLAKAYLNYLYSPLGQELAAKYNFRPRNAKAAAKYAYKFPKLK
CCCEEEEEHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCEE
LFTIGDVFGGWAKAQKTHFVNGAIFDQIYAEKP
EEEECCHHCCHHHHHHHHCCCHHHHHHHHCCCC
>Mature Secondary Structure
MKKIWGVTFAILLGFGAGTANAAKDFLNVSYDPTREFYQEYNQSFYTYWKGRTGQEVDFK
CCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHCCCEEEEECCCCCCCCCCH
QSHGGSGKQARAVIDGLQADVVTLALANDIDEIANAGLIKKNWQKDFPNNSAPYTSTIVF
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCCCCCCEEEEEE
LVRKGNPKNIKDWNDLTKPGVEIITPNPKTGGAPRWIYLAAWGYALKQPGGNDSKARELV
EEECCCCCCCCCCHHHCCCCCEEECCCCCCCCCCCEEEEEECCHHHCCCCCCHHHHHHHH
KKLYHNVKVLDSGARGSLTTFAERGIGDVLLSWENEALLATQGPGKDKYEIIYPSISILA
HHHHCCEEEEECCCCCCHHHHHHCCCCCEEEECCCCEEEEECCCCCCCEEEECCCCEEEE
EPSVAIVDKTVDRDGNRNLAKAYLNYLYSPLGQELAAKYNFRPRNAKAAAKYAYKFPKLK
CCCEEEEEHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCEE
LFTIGDVFGGWAKAQKTHFVNGAIFDQIYAEKP
EEEECCHHCCHHHHHHHHCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3158524; 2002055; 8346018; 9278503; 9298646; 8774726 [H]