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The map label for this gene is ftsI [H]

Identifier: 50086344

GI number: 50086344

Start: 3275147

End: 3277012

Strand: Reverse

Name: ftsI [H]

Synonym: ACIAD3366

Alternate gene names: 50086344

Gene position: 3277012-3275147 (Counterclockwise)

Preceding gene: 50086345

Following gene: 50086343

Centisome position: 91.06

GC content: 45.23

Gene sequence:

>1866_bases
CTGAGCAAAAACACTAAGGCCTGCTGTATGATAGACAAGCGAATCAAGCAAACAAGAAAAAAACAGCAATCTATTTCTGA
AAAGCCCTCATTGGCTTTTGATATGTGGCGTTTCTATCTGCTGTGGGGCGTTGTACTTCTATGTTTTGTGGTGTTGATTG
CGCGTGCTTTTTATGTGCAAGTGATCAATAAAGATTTCCTGCAAAATAAAGCCAATGCCAATATCTTAAGAACAGAAAAG
ATTAAGGCAATGCGTGGTGTCATCAGTGATCGTCACGGGGTTCCTTTAGCAATTAGTACGCCTGTGATGAAAGTGGTGAT
TGATCCGCGCGATTATTTTGAAACCAAAAAAGAATATGATGATATATCGGCAGAACTGCGCAAAGATCCAAATAATCGTA
AGCTTAAGCGTCAATTACCTGATAAAAATCTGAACCTGGATGAACTTGCAGATGCTGTAGGTATGGACCGTGCGGAATTG
AAAAAACAGATGTATGCTCGTCCGCGTTCACGTTATCTTGTACTACAAAAGGAAATCCCACCGCAGCAGGCAGATTTGAT
TCTAAAACGTAATTTTCAGGGTGTTTATACCGAGAAAAATTACAAGAGATATTATCCGCAACCACAACCCAATGCACAAA
TTATTGGCCTGACTAACAGTGAAGGCGTTGGTGTCGAAGGTTTGGAAATGCAGTTGAATAAGCAGCTTTCGGGTGTGGAT
GGCGAGCAGAAAGTTATCCGTGACAAGCGCGGAAACCGTCTGAAAATCTCGGAAGTGATTCGAGAAGGTGAGCCTGGCGA
AAATATTACCCTGAGTATTGATTCACGCTTGCAATACATCATGTACCGCGAGTTGACGGCAGCAGGGGTTGCCAATAATG
CTCGTTCTGCTTCAGCCATTGCGGTGGATGTTAAAACAGGCGAAATTCTGGCCATGGCCAGTTGGCCGTCGTATAACCCG
AATGACAAAAATGGTTTGTCCAATAAGGATGCCATGCGTAACCGCGGTGCCATTGACATGTTTGAGCCAGGTTCAACCAT
GAAGCCGTTTACAGTGTCCGCAGCCTTAGAAAGTGGACAGTACACGCCCAATACCATTGTAAACACGTCACCAGGTTCGA
TGCGTCTTGGTTGGCATACCATTCGTGATACGCATAACTACGGCGCTCTTACTTTAACAGGCGTTATTGTAAAATCCTCA
AACGTCGGTTCGGCAAAAATTGCGCTGTCTTTACCCAAAGACGCATTACCGACTTTCTATAGACGGGTTGGATTTGGACA
GCGCTCTGCGGTTCACTTTCCTGGTGAGAGTGGTGGTCTGATTCTGCCAAATAGTAAGCTTACACCTTCTCAGATTGGTA
CGATGGCATATGGCTATGGTTTAAACGCCACAATTCTTCAGTTGGCGCAGGGTTATGCCATGTTGGCCAATCATGGTGTA
AAAATGCCGCTCAGCTTACGTAAGCTGGATGAACCCCCTAAAGGTGAGCAAGTATTAAACCCGAAAATTGCCGATCAGGT
ACTTAGTATGCTTGAGCAAGTGACGATGCCAGGTGGAACCGCCACACAAGCGAATATACCTGGTTATCGTGTGGGTGGTA
AAACAGGAACTGCGCATAAATTACGTCCAGATGGTAAGGGTTATGCACAGAATCAATACCGTGCCTTATTTGCGGGGGTT
GCACCTGTGAGTGATCCGCGTCTGGCAATGATTATTGTGGTGGAAAATCCGCAAGGTCGTTATTACGGTGGTTTGGTCGC
TGCGCCTGTCTTTACACGTATCATGCAGGAATCTCTCAGACTGATGAATGTGCCGCTGGATAAGCCCCTAGATACACCAA
GCAACCCAATTTTAAAACGCCAGTAA

Upstream 100 bases:

>100_bases
TTTGGTGCAACAGCGCAAATTGGTTCACGGGCAGTCACCCAGTTACGCATGTTTTCACCTCCTGCGGCACAAACTGCCGT
TATTTCGTTGCCAATGACCC

Downstream 100 bases:

>100_bases
GAGGTAAGTGATGAGCGTCTCATTTGAAATGATCTCTGCGATTGATGCAGCGATTGAATGGCGAGATCAGCCTTTTCAGG
GATTTTGCCTCGACAGTCGA

Product: septum formation, penicillin binding protein 3, peptidoglycan synthetase

Products: NA

Alternate protein names: Penicillin-binding protein 3; PBP-3; Peptidoglycan glycosyltransferase 3 [H]

Number of amino acids: Translated: 621; Mature: 620

Protein sequence:

>621_residues
MSKNTKACCMIDKRIKQTRKKQQSISEKPSLAFDMWRFYLLWGVVLLCFVVLIARAFYVQVINKDFLQNKANANILRTEK
IKAMRGVISDRHGVPLAISTPVMKVVIDPRDYFETKKEYDDISAELRKDPNNRKLKRQLPDKNLNLDELADAVGMDRAEL
KKQMYARPRSRYLVLQKEIPPQQADLILKRNFQGVYTEKNYKRYYPQPQPNAQIIGLTNSEGVGVEGLEMQLNKQLSGVD
GEQKVIRDKRGNRLKISEVIREGEPGENITLSIDSRLQYIMYRELTAAGVANNARSASAIAVDVKTGEILAMASWPSYNP
NDKNGLSNKDAMRNRGAIDMFEPGSTMKPFTVSAALESGQYTPNTIVNTSPGSMRLGWHTIRDTHNYGALTLTGVIVKSS
NVGSAKIALSLPKDALPTFYRRVGFGQRSAVHFPGESGGLILPNSKLTPSQIGTMAYGYGLNATILQLAQGYAMLANHGV
KMPLSLRKLDEPPKGEQVLNPKIADQVLSMLEQVTMPGGTATQANIPGYRVGGKTGTAHKLRPDGKGYAQNQYRALFAGV
APVSDPRLAMIIVVENPQGRYYGGLVAAPVFTRIMQESLRLMNVPLDKPLDTPSNPILKRQ

Sequences:

>Translated_621_residues
MSKNTKACCMIDKRIKQTRKKQQSISEKPSLAFDMWRFYLLWGVVLLCFVVLIARAFYVQVINKDFLQNKANANILRTEK
IKAMRGVISDRHGVPLAISTPVMKVVIDPRDYFETKKEYDDISAELRKDPNNRKLKRQLPDKNLNLDELADAVGMDRAEL
KKQMYARPRSRYLVLQKEIPPQQADLILKRNFQGVYTEKNYKRYYPQPQPNAQIIGLTNSEGVGVEGLEMQLNKQLSGVD
GEQKVIRDKRGNRLKISEVIREGEPGENITLSIDSRLQYIMYRELTAAGVANNARSASAIAVDVKTGEILAMASWPSYNP
NDKNGLSNKDAMRNRGAIDMFEPGSTMKPFTVSAALESGQYTPNTIVNTSPGSMRLGWHTIRDTHNYGALTLTGVIVKSS
NVGSAKIALSLPKDALPTFYRRVGFGQRSAVHFPGESGGLILPNSKLTPSQIGTMAYGYGLNATILQLAQGYAMLANHGV
KMPLSLRKLDEPPKGEQVLNPKIADQVLSMLEQVTMPGGTATQANIPGYRVGGKTGTAHKLRPDGKGYAQNQYRALFAGV
APVSDPRLAMIIVVENPQGRYYGGLVAAPVFTRIMQESLRLMNVPLDKPLDTPSNPILKRQ
>Mature_620_residues
SKNTKACCMIDKRIKQTRKKQQSISEKPSLAFDMWRFYLLWGVVLLCFVVLIARAFYVQVINKDFLQNKANANILRTEKI
KAMRGVISDRHGVPLAISTPVMKVVIDPRDYFETKKEYDDISAELRKDPNNRKLKRQLPDKNLNLDELADAVGMDRAELK
KQMYARPRSRYLVLQKEIPPQQADLILKRNFQGVYTEKNYKRYYPQPQPNAQIIGLTNSEGVGVEGLEMQLNKQLSGVDG
EQKVIRDKRGNRLKISEVIREGEPGENITLSIDSRLQYIMYRELTAAGVANNARSASAIAVDVKTGEILAMASWPSYNPN
DKNGLSNKDAMRNRGAIDMFEPGSTMKPFTVSAALESGQYTPNTIVNTSPGSMRLGWHTIRDTHNYGALTLTGVIVKSSN
VGSAKIALSLPKDALPTFYRRVGFGQRSAVHFPGESGGLILPNSKLTPSQIGTMAYGYGLNATILQLAQGYAMLANHGVK
MPLSLRKLDEPPKGEQVLNPKIADQVLSMLEQVTMPGGTATQANIPGYRVGGKTGTAHKLRPDGKGYAQNQYRALFAGVA
PVSDPRLAMIIVVENPQGRYYGGLVAAPVFTRIMQESLRLMNVPLDKPLDTPSNPILKRQ

Specific function: Cell wall formation. Essential for the formation of a septum of the murein sacculus. Synthesis of cross-linked peptidoglycan from the lipid intermediates [H]

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein; Periplasmic side. Note=The bulk of the molecule, except for the N-terminal membrane anchor region, protrudes into the periplasmic space, where it acts on murein (By similarity) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786272, Length=595, Percent_Identity=40.3361344537815, Blast_Score=422, Evalue=1e-119,
Organism=Escherichia coli, GI1786854, Length=633, Percent_Identity=24.8025276461295, Blast_Score=112, Evalue=6e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005311
- InterPro:   IPR001460 [H]

Pfam domain/function: PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: =2.4.1.129 [H]

Molecular weight: Translated: 68911; Mature: 68779

Theoretical pI: Translated: 10.28; Mature: 10.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKNTKACCMIDKRIKQTRKKQQSISEKPSLAFDMWRFYLLWGVVLLCFVVLIARAFYVQ
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VINKDFLQNKANANILRTEKIKAMRGVISDRHGVPLAISTPVMKVVIDPRDYFETKKEYD
HHHHHHHHCCCCCCEEEHHHHHHHHHHHCCCCCCCEEECCCEEEEEECCHHHHHHHHHHH
DISAELRKDPNNRKLKRQLPDKNLNLDELADAVGMDRAELKKQMYARPRSRYLVLQKEIP
HHHHHHHCCCCCCHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEECCCC
PQQADLILKRNFQGVYTEKNYKRYYPQPQPNAQIIGLTNSEGVGVEGLEMQLNKQLSGVD
CHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCC
GEQKVIRDKRGNRLKISEVIREGEPGENITLSIDSRLQYIMYRELTAAGVANNARSASAI
CHHHHHHHCCCCEEEHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHCCCCCCCCCCEE
AVDVKTGEILAMASWPSYNPNDKNGLSNKDAMRNRGAIDMFEPGSTMKPFTVSAALESGQ
EEEECCCCEEEEECCCCCCCCCCCCCCCHHHHHCCCCEEEECCCCCCCCEEEEEHHHCCC
YTPNTIVNTSPGSMRLGWHTIRDTHNYGALTLTGVIVKSSNVGSAKIALSLPKDALPTFY
CCCCCEEECCCCCEEECEEEECCCCCCCCEEEEEEEEECCCCCCEEEEEECCHHHHHHHH
RRVGFGQRSAVHFPGESGGLILPNSKLTPSQIGTMAYGYGLNATILQLAQGYAMLANHGV
HHHCCCCCCEEECCCCCCCEECCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCC
KMPLSLRKLDEPPKGEQVLNPKIADQVLSMLEQVTMPGGTATQANIPGYRVGGKTGTAHK
CCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCCCC
LRPDGKGYAQNQYRALFAGVAPVSDPRLAMIIVVENPQGRYYGGLVAAPVFTRIMQESLR
CCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHH
LMNVPLDKPLDTPSNPILKRQ
HHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SKNTKACCMIDKRIKQTRKKQQSISEKPSLAFDMWRFYLLWGVVLLCFVVLIARAFYVQ
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VINKDFLQNKANANILRTEKIKAMRGVISDRHGVPLAISTPVMKVVIDPRDYFETKKEYD
HHHHHHHHCCCCCCEEEHHHHHHHHHHHCCCCCCCEEECCCEEEEEECCHHHHHHHHHHH
DISAELRKDPNNRKLKRQLPDKNLNLDELADAVGMDRAELKKQMYARPRSRYLVLQKEIP
HHHHHHHCCCCCCHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEECCCC
PQQADLILKRNFQGVYTEKNYKRYYPQPQPNAQIIGLTNSEGVGVEGLEMQLNKQLSGVD
CHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCC
GEQKVIRDKRGNRLKISEVIREGEPGENITLSIDSRLQYIMYRELTAAGVANNARSASAI
CHHHHHHHCCCCEEEHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHCCCCCCCCCCEE
AVDVKTGEILAMASWPSYNPNDKNGLSNKDAMRNRGAIDMFEPGSTMKPFTVSAALESGQ
EEEECCCCEEEEECCCCCCCCCCCCCCCHHHHHCCCCEEEECCCCCCCCEEEEEHHHCCC
YTPNTIVNTSPGSMRLGWHTIRDTHNYGALTLTGVIVKSSNVGSAKIALSLPKDALPTFY
CCCCCEEECCCCCEEECEEEECCCCCCCCEEEEEEEEECCCCCCEEEEEECCHHHHHHHH
RRVGFGQRSAVHFPGESGGLILPNSKLTPSQIGTMAYGYGLNATILQLAQGYAMLANHGV
HHHCCCCCCEEECCCCCCCEECCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCC
KMPLSLRKLDEPPKGEQVLNPKIADQVLSMLEQVTMPGGTATQANIPGYRVGGKTGTAHK
CCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCCCC
LRPDGKGYAQNQYRALFAGVAPVSDPRLAMIIVVENPQGRYYGGLVAAPVFTRIMQESLR
CCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHH
LMNVPLDKPLDTPSNPILKRQ
HHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]