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The map label for this gene is murE [H]

Identifier: 50086343

GI number: 50086343

Start: 3273640

End: 3275139

Strand: Reverse

Name: murE [H]

Synonym: ACIAD3365

Alternate gene names: 50086343

Gene position: 3275139-3273640 (Counterclockwise)

Preceding gene: 50086344

Following gene: 50086342

Centisome position: 91.01

GC content: 45.27

Gene sequence:

>1500_bases
GTGATGAGCGTCTCATTTGAAATGATCTCTGCGATTGATGCAGCGATTGAATGGCGAGATCAGCCTTTTCAGGGATTTTG
CCTCGACAGTCGATATATTCAGAAAGATCAGCTTTTTATTGCGCTAACCAGTTATTCACAACCCGAAAAAACCCGAATTT
TTGCAGAGACAGCACTGGCTCAAGGTGCGCTGGGTATCATCAGTGAGCAGCCTTTAGGGCTTGAGCGTGAGTGGGTTTGC
GCTGATGTGCGTCATCGAATGGGGCAATGGCAGAAAAACTATTTGCAAAAAACAGAGCCTGTCAATGCTGCACGAATTAT
CGCAGTTACGGGCACCAATGGAAAAACCACCATTTCGCGTTTGGTTGCAGAATTACTGACCTTACAGCAGCAGCGTTGTG
CGGTCATGGGAACCACAGGCAATGGTATTTTACCGCATCTTAATCCATCAACACACACCACGCTGGATGCTTTGCAATTG
CAAAGCGCCTTGCATCAATATGTAAAAGAAGGTGCACGTTTTGCATCTTTGGAAGCCAGTTCTCATGGCTTAGAGCAAGG
GCGACTGAATGGCTGTGACATTGAAATTGCGGTTTACAGTAATTTGAGTCGTGATCATCTGGATTATCATGGCACTTTAG
CGGCCTATGCAGAAGCCAAATCACGCTTATTTGCTTTTAAATCTTTAAAGGTCGCCGTGATCAATCAGGATGATGCATAT
GCTGAACTAATGAAAGCAGTAGCAGCTCAAAACCCAGCCCAGCCTCAGATTCTGACTTATTCGCTTACACAGTCTGCGGA
CTATAGCGTAAAAGAATTACAATACAGCTTGTCTGGTGCTCGATTTATTCTGGTGGGCCCAAAAGGTGAGTATTTGGTTG
AAAGTCCATTGCTGGGTCATTTCAATGTTGAAAATCTAATTGCCGCGCTGATTGCAGCCGAGCAAGCTGGTTTTGCGCTT
GAAGAGCTTGTAAAAACTGTACCGCAATTAATTGGGGCACCAGGTCGTATGCAAGTCATTCGGGATCAGGATCGACTTTT
TGTTGTGGACTATGCGCATACACCCGATGCTTTGAATCAGGTACTTAAAACATTACGTCGTCATGTACAGCAGCAATTGT
GGGCAGTGTTTGGGTGTGGTGGAGATCGCGATCGCGGCAAGCGTCCGCTTATGACGCAGGCCGCTTTGAATGGCGCAAAT
CCTGTCATTTTGACCTCAGATAATCCTCGTACTGAAAACCCAAAGCAGATTTTTGCGGATATGAAGACTGGAATCGATTT
TTCACACCATCAAATCTATGAAATTCACGATCGCCGAGAAGCAATCAAATATGTGGTTCAGCATGCACAGGCAGGCGACA
TTGTGGTCATTGCGGGTAAAGGACATGAAAATTATCAGGAAATCCAAGGTGTTCGTCACTGGTTTGATGATGTAACAGAA
GTCCAGTCCGCTATAAATACACAAACTCATTCAGTTCATTCTGCGTATCCTGCACATTAG

Upstream 100 bases:

>100_bases
ACACGTATCATGCAGGAATCTCTCAGACTGATGAATGTGCCGCTGGATAAGCCCCTAGATACACCAAGCAACCCAATTTT
AAAACGCCAGTAAGAGGTAA

Downstream 100 bases:

>100_bases
GATTTTTTATGCATACTTCTACAACCAGCACGGTTCCTTTAGCACCGTGGGATGCTTCTCAGTTACAACAGGCAACGCAA
GGCTACTGGCATAATAATCG

Product: UDP-N-acetylmuramoylalanyl-D-glutamate-2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 499; Mature: 499

Protein sequence:

>499_residues
MMSVSFEMISAIDAAIEWRDQPFQGFCLDSRYIQKDQLFIALTSYSQPEKTRIFAETALAQGALGIISEQPLGLEREWVC
ADVRHRMGQWQKNYLQKTEPVNAARIIAVTGTNGKTTISRLVAELLTLQQQRCAVMGTTGNGILPHLNPSTHTTLDALQL
QSALHQYVKEGARFASLEASSHGLEQGRLNGCDIEIAVYSNLSRDHLDYHGTLAAYAEAKSRLFAFKSLKVAVINQDDAY
AELMKAVAAQNPAQPQILTYSLTQSADYSVKELQYSLSGARFILVGPKGEYLVESPLLGHFNVENLIAALIAAEQAGFAL
EELVKTVPQLIGAPGRMQVIRDQDRLFVVDYAHTPDALNQVLKTLRRHVQQQLWAVFGCGGDRDRGKRPLMTQAALNGAN
PVILTSDNPRTENPKQIFADMKTGIDFSHHQIYEIHDRREAIKYVVQHAQAGDIVVIAGKGHENYQEIQGVRHWFDDVTE
VQSAINTQTHSVHSAYPAH

Sequences:

>Translated_499_residues
MMSVSFEMISAIDAAIEWRDQPFQGFCLDSRYIQKDQLFIALTSYSQPEKTRIFAETALAQGALGIISEQPLGLEREWVC
ADVRHRMGQWQKNYLQKTEPVNAARIIAVTGTNGKTTISRLVAELLTLQQQRCAVMGTTGNGILPHLNPSTHTTLDALQL
QSALHQYVKEGARFASLEASSHGLEQGRLNGCDIEIAVYSNLSRDHLDYHGTLAAYAEAKSRLFAFKSLKVAVINQDDAY
AELMKAVAAQNPAQPQILTYSLTQSADYSVKELQYSLSGARFILVGPKGEYLVESPLLGHFNVENLIAALIAAEQAGFAL
EELVKTVPQLIGAPGRMQVIRDQDRLFVVDYAHTPDALNQVLKTLRRHVQQQLWAVFGCGGDRDRGKRPLMTQAALNGAN
PVILTSDNPRTENPKQIFADMKTGIDFSHHQIYEIHDRREAIKYVVQHAQAGDIVVIAGKGHENYQEIQGVRHWFDDVTE
VQSAINTQTHSVHSAYPAH
>Mature_499_residues
MMSVSFEMISAIDAAIEWRDQPFQGFCLDSRYIQKDQLFIALTSYSQPEKTRIFAETALAQGALGIISEQPLGLEREWVC
ADVRHRMGQWQKNYLQKTEPVNAARIIAVTGTNGKTTISRLVAELLTLQQQRCAVMGTTGNGILPHLNPSTHTTLDALQL
QSALHQYVKEGARFASLEASSHGLEQGRLNGCDIEIAVYSNLSRDHLDYHGTLAAYAEAKSRLFAFKSLKVAVINQDDAY
AELMKAVAAQNPAQPQILTYSLTQSADYSVKELQYSLSGARFILVGPKGEYLVESPLLGHFNVENLIAALIAAEQAGFAL
EELVKTVPQLIGAPGRMQVIRDQDRLFVVDYAHTPDALNQVLKTLRRHVQQQLWAVFGCGGDRDRGKRPLMTQAALNGAN
PVILTSDNPRTENPKQIFADMKTGIDFSHHQIYEIHDRREAIKYVVQHAQAGDIVVIAGKGHENYQEIQGVRHWFDDVTE
VQSAINTQTHSVHSAYPAH

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=476, Percent_Identity=38.2352941176471, Blast_Score=251, Evalue=1e-67,
Organism=Escherichia coli, GI1786274, Length=360, Percent_Identity=23.8888888888889, Blast_Score=65, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 55410; Mature: 55410

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMSVSFEMISAIDAAIEWRDQPFQGFCLDSRYIQKDQLFIALTSYSQPEKTRIFAETALA
CCCCHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHH
QGALGIISEQPLGLEREWVCADVRHRMGQWQKNYLQKTEPVNAARIIAVTGTNGKTTISR
CCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHH
LVAELLTLQQQRCAVMGTTGNGILPHLNPSTHTTLDALQLQSALHQYVKEGARFASLEAS
HHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCC
SHGLEQGRLNGCDIEIAVYSNLSRDHLDYHGTLAAYAEAKSRLFAFKSLKVAVINQDDAY
CCCCCCCCCCCCEEEEEEECCCCHHCCCCCCHHHHHHHHHHHHHHEECEEEEEECCCHHH
AELMKAVAAQNPAQPQILTYSLTQSADYSVKELQYSLSGARFILVGPKGEYLVESPLLGH
HHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHCCCCEEEEECCCCCEEECCCCCCC
FNVENLIAALIAAEQAGFALEELVKTVPQLIGAPGRMQVIRDQDRLFVVDYAHTPDALNQ
CCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEEEECCCCHHHHH
VLKTLRRHVQQQLWAVFGCGGDRDRGKRPLMTQAALNGANPVILTSDNPRTENPKQIFAD
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCCCEEEECCCCCCCCHHHHHHH
MKTGIDFSHHQIYEIHDRREAIKYVVQHAQAGDIVVIAGKGHENYQEIQGVRHWFDDVTE
HHHCCCCCCCHHEEHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHH
VQSAINTQTHSVHSAYPAH
HHHHHCCHHCCCCCCCCCC
>Mature Secondary Structure
MMSVSFEMISAIDAAIEWRDQPFQGFCLDSRYIQKDQLFIALTSYSQPEKTRIFAETALA
CCCCHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHH
QGALGIISEQPLGLEREWVCADVRHRMGQWQKNYLQKTEPVNAARIIAVTGTNGKTTISR
CCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHH
LVAELLTLQQQRCAVMGTTGNGILPHLNPSTHTTLDALQLQSALHQYVKEGARFASLEAS
HHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCC
SHGLEQGRLNGCDIEIAVYSNLSRDHLDYHGTLAAYAEAKSRLFAFKSLKVAVINQDDAY
CCCCCCCCCCCCEEEEEEECCCCHHCCCCCCHHHHHHHHHHHHHHEECEEEEEECCCHHH
AELMKAVAAQNPAQPQILTYSLTQSADYSVKELQYSLSGARFILVGPKGEYLVESPLLGH
HHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHCCCCEEEEECCCCCEEECCCCCCC
FNVENLIAALIAAEQAGFALEELVKTVPQLIGAPGRMQVIRDQDRLFVVDYAHTPDALNQ
CCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEEEECCCCHHHHH
VLKTLRRHVQQQLWAVFGCGGDRDRGKRPLMTQAALNGANPVILTSDNPRTENPKQIFAD
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCCCEEEECCCCCCCCHHHHHHH
MKTGIDFSHHQIYEIHDRREAIKYVVQHAQAGDIVVIAGKGHENYQEIQGVRHWFDDVTE
HHHCCCCCCCHHEEHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHH
VQSAINTQTHSVHSAYPAH
HHHHHCCHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043; 7486937 [H]