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The map label for this gene is amiF [H]

Identifier: 50086321

GI number: 50086321

Start: 3243530

End: 3244393

Strand: Direct

Name: amiF [H]

Synonym: ACIAD3340

Alternate gene names: 50086321

Gene position: 3243530-3244393 (Clockwise)

Preceding gene: 50086313

Following gene: 50086322

Centisome position: 90.13

GC content: 44.56

Gene sequence:

>864_bases
ATGTTAAGCAACTTTGGTATCGCCGGCATACAAATGCAGGTCTCTGCATTTACCAGTAATGTAGAACAAATGGGTAATTA
TATGCGTCATATTCGCATGCGTTATCCATGGGTCAAAATGGTTTTATTTTCAGAACTCGCCCCGTTAGGACCAAAGCATA
GTACCGCAGAAGTTTTTCCAAGCCAGACCGAAGCGCAATTGGCCGAACTTGCCCGTGAAACGGGACTATGGCTGATTCCA
GGTTCATTGTTTGAACGTGTCGAAAGCCCAGAAGGTGACGTCATTTACAACACGTTATCTGTGATTAATCCTCAAGGACA
AATTGTTGCACGTTATCGCAAGATGTTTCCATTTCGTCCTTATGAAAAAAGTGTCGCTGGTGGTAATGAGTTCTGTGTTT
TTGACGTGCCAGAGGTGGGGCGCTTTGGCGTTTCGATCTGCTATGACATGTGGTTCCCAGAAACAACGCGTACTTTGGTC
GCCATGGGCGCGGAAGTGATTTTGCATCCAACCATGACCGATACCATTGACCGTGATATTGAGCTTTCAATTGCACGGGC
ATCCGCTGCACAAAATCAGGTTTATTTTATTGATATTAACGGTGTTGGAGACGGCGGTATTGGTCGATCTATCGTAATTG
ATCCATCTGGTTATGTTCTGCATCAATCTGAGGCAGGCCCAGAAATTATTCCTATTGATGTTGACCTTGAAAAAGTACGC
AGGGAGCGTAGACGTGGCTTACGCAGCCTAGGCCAGCCTCTAAAAAGTTTTAGAGATCGTGACTGTAATTTTACAGTTTA
TCAAGATACGACAGCTTTCCCTTATCTGGATAGTTTAGGTCCATTGGTCAAACCAGACGACTAA

Upstream 100 bases:

>100_bases
GCGGATACAAATCAATTCCAGCGATGATGCATTATGTAATTTAACTGTAGGTTCGATGCTTGATGTTCATGATCTCTGGA
AAAGCAAGTAAGGAGAAAAT

Downstream 100 bases:

>100_bases
ATTGATTAAAAACTAAAACGTAAAAGATAAAGAAAGATTGATAACAAAGATATAAGCTTATGACGAATACAAATTCATTA
AGCCAGCATCGTAGCGTATG

Product: hydrolase

Products: NA

Alternate protein names: Formamide amidohydrolase [H]

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MLSNFGIAGIQMQVSAFTSNVEQMGNYMRHIRMRYPWVKMVLFSELAPLGPKHSTAEVFPSQTEAQLAELARETGLWLIP
GSLFERVESPEGDVIYNTLSVINPQGQIVARYRKMFPFRPYEKSVAGGNEFCVFDVPEVGRFGVSICYDMWFPETTRTLV
AMGAEVILHPTMTDTIDRDIELSIARASAAQNQVYFIDINGVGDGGIGRSIVIDPSGYVLHQSEAGPEIIPIDVDLEKVR
RERRRGLRSLGQPLKSFRDRDCNFTVYQDTTAFPYLDSLGPLVKPDD

Sequences:

>Translated_287_residues
MLSNFGIAGIQMQVSAFTSNVEQMGNYMRHIRMRYPWVKMVLFSELAPLGPKHSTAEVFPSQTEAQLAELARETGLWLIP
GSLFERVESPEGDVIYNTLSVINPQGQIVARYRKMFPFRPYEKSVAGGNEFCVFDVPEVGRFGVSICYDMWFPETTRTLV
AMGAEVILHPTMTDTIDRDIELSIARASAAQNQVYFIDINGVGDGGIGRSIVIDPSGYVLHQSEAGPEIIPIDVDLEKVR
RERRRGLRSLGQPLKSFRDRDCNFTVYQDTTAFPYLDSLGPLVKPDD
>Mature_287_residues
MLSNFGIAGIQMQVSAFTSNVEQMGNYMRHIRMRYPWVKMVLFSELAPLGPKHSTAEVFPSQTEAQLAELARETGLWLIP
GSLFERVESPEGDVIYNTLSVINPQGQIVARYRKMFPFRPYEKSVAGGNEFCVFDVPEVGRFGVSICYDMWFPETTRTLV
AMGAEVILHPTMTDTIDRDIELSIARASAAQNQVYFIDINGVGDGGIGRSIVIDPSGYVLHQSEAGPEIIPIDVDLEKVR
RERRRGLRSLGQPLKSFRDRDCNFTVYQDTTAFPYLDSLGPLVKPDD

Specific function: Is an aliphatic amidase with a restricted substrate specificity, as it only hydrolyzes formamide [H]

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI9910460, Length=267, Percent_Identity=30.3370786516854, Blast_Score=104, Evalue=7e-23,
Organism=Homo sapiens, GI297632348, Length=197, Percent_Identity=30.4568527918782, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI297632350, Length=197, Percent_Identity=30.4568527918782, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI5031947, Length=194, Percent_Identity=30.9278350515464, Blast_Score=91, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6323383, Length=279, Percent_Identity=26.8817204301075, Blast_Score=85, Evalue=1e-17,
Organism=Drosophila melanogaster, GI21355835, Length=217, Percent_Identity=28.5714285714286, Blast_Score=92, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022843
- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.1.49 [H]

Molecular weight: Translated: 32192; Mature: 32192

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS50263 CN_HYDROLASE ; PS01227 UPF0012

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSNFGIAGIQMQVSAFTSNVEQMGNYMRHIRMRYPWVKMVLFSELAPLGPKHSTAEVFP
CCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCHHCCC
SQTEAQLAELARETGLWLIPGSLFERVESPEGDVIYNTLSVINPQGQIVARYRKMFPFRP
CCHHHHHHHHHHHCCCEEECHHHHHHHCCCCCCEEEEHHHHCCCCHHHHHHHHHHCCCCC
YEKSVAGGNEFCVFDVPEVGRFGVSICYDMWFPETTRTLVAMGAEVILHPTMTDTIDRDI
CHHHCCCCCEEEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHCCEEEEECCHHHHCCCCE
ELSIARASAAQNQVYFIDINGVGDGGIGRSIVIDPSGYVLHQSEAGPEIIPIDVDLEKVR
EEEEEEHHCCCCEEEEEEEECCCCCCCCCEEEECCCCCEEECCCCCCEEEEECCCHHHHH
RERRRGLRSLGQPLKSFRDRDCNFTVYQDTTAFPYLDSLGPLVKPDD
HHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHCCCCCCCCC
>Mature Secondary Structure
MLSNFGIAGIQMQVSAFTSNVEQMGNYMRHIRMRYPWVKMVLFSELAPLGPKHSTAEVFP
CCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCHHCCC
SQTEAQLAELARETGLWLIPGSLFERVESPEGDVIYNTLSVINPQGQIVARYRKMFPFRP
CCHHHHHHHHHHHCCCEEECHHHHHHHCCCCCCEEEEHHHHCCCCHHHHHHHHHHCCCCC
YEKSVAGGNEFCVFDVPEVGRFGVSICYDMWFPETTRTLVAMGAEVILHPTMTDTIDRDI
CHHHCCCCCEEEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHCCEEEEECCHHHHCCCCE
ELSIARASAAQNQVYFIDINGVGDGGIGRSIVIDPSGYVLHQSEAGPEIIPIDVDLEKVR
EEEEEEHHCCCCEEEEEEEECCCCCCCCCEEEECCCCCEEECCCCCCEEEEECCCHHHHH
RERRRGLRSLGQPLKSFRDRDCNFTVYQDTTAFPYLDSLGPLVKPDD
HHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA