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The map label for this gene is trmD

Identifier: 50086296

GI number: 50086296

Start: 3217565

End: 3218314

Strand: Reverse

Name: trmD

Synonym: ACIAD3311

Alternate gene names: 50086296

Gene position: 3218314-3217565 (Counterclockwise)

Preceding gene: 50086297

Following gene: 50086295

Centisome position: 89.43

GC content: 42.8

Gene sequence:

>750_bases
ATGTTTTTTGCAGTCATTACACTTTTTCCAGAAATGTTTGAGGCAATCACTGCTTATGGAATCAGCGGACGTGCAACCAA
ACGTCAGATTGCAACAGTTAGCTGTATTAATCCACGTGATTTCGCAGAGGGTAACTACAAACGGGTGGATGAACGTCCAT
TTGGTGGTGGCCCAGGTATGGTGATGATGGCGGAGCCTCTGGCAAAAGCGATTATTCATGCCAAACAGCTTGCTGAGCAA
GCAGGTTGTGTTCATGCGCCAGTGGTATATATGTCGCCACAAGGCAAAACCTTAAATGAACACGCGGTACAACAATTTGT
CGATTATGACGGTTTGATTGTGTTGTGTGGTCGTTATGAAGGTGTCGATGAGCGTTTGATCCAGCACTATGTTGATCAGG
AATGGTCAATTGGAGATTATGTATTATCGGGAGGTGAGCTTCCTGCGATGGTGTTACTGGATAGTATTATTCGGCGGTTA
CCAGATGCGATGTCTGACGAGCAATCTCATATACAAGATTCTTTTGTGGATGGTCTTTTAGATTGTCCACAATATACCAA
GCCTGACCATTTTGAAGGGTTGGATGTTCCTGAAGTTCTAAAATCAGGACATCATGCCAATATTGAAAAATGGCGTTTTT
TGCAGCGGTATCAACGTACTTTAGATCGTCGACCTGAACTGGTTGAAAAAGTGACGTTGACCAAGCAGCAAAGAAAATGG
CTAACATTTTTAGATGACTCAAAAAATTGA

Upstream 100 bases:

>100_bases
GGGTTGATCTGGAAGCAGGCCGTATTTATGTCAATTGGGGCGTAGATTACTAATCCACTTGATGGATGGCAAACGCAGTT
GGAAAATATAGGAGTCTGCG

Downstream 100 bases:

>100_bases
GTGATCTGATATAGGCTCTTTATTTTTATACCTTGAGTATAAGTAATAGAGGGAAAGTTAAACGGGTTGATATGCGTTTT
AGCCTCTGTCCCCAGCGGTG

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MFFAVITLFPEMFEAITAYGISGRATKRQIATVSCINPRDFAEGNYKRVDERPFGGGPGMVMMAEPLAKAIIHAKQLAEQ
AGCVHAPVVYMSPQGKTLNEHAVQQFVDYDGLIVLCGRYEGVDERLIQHYVDQEWSIGDYVLSGGELPAMVLLDSIIRRL
PDAMSDEQSHIQDSFVDGLLDCPQYTKPDHFEGLDVPEVLKSGHHANIEKWRFLQRYQRTLDRRPELVEKVTLTKQQRKW
LTFLDDSKN

Sequences:

>Translated_249_residues
MFFAVITLFPEMFEAITAYGISGRATKRQIATVSCINPRDFAEGNYKRVDERPFGGGPGMVMMAEPLAKAIIHAKQLAEQ
AGCVHAPVVYMSPQGKTLNEHAVQQFVDYDGLIVLCGRYEGVDERLIQHYVDQEWSIGDYVLSGGELPAMVLLDSIIRRL
PDAMSDEQSHIQDSFVDGLLDCPQYTKPDHFEGLDVPEVLKSGHHANIEKWRFLQRYQRTLDRRPELVEKVTLTKQQRKW
LTFLDDSKN
>Mature_249_residues
MFFAVITLFPEMFEAITAYGISGRATKRQIATVSCINPRDFAEGNYKRVDERPFGGGPGMVMMAEPLAKAIIHAKQLAEQ
AGCVHAPVVYMSPQGKTLNEHAVQQFVDYDGLIVLCGRYEGVDERLIQHYVDQEWSIGDYVLSGGELPAMVLLDSIIRRL
PDAMSDEQSHIQDSFVDGLLDCPQYTKPDHFEGLDVPEVLKSGHHANIEKWRFLQRYQRTLDRRPELVEKVTLTKQQRKW
LTFLDDSKN

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=242, Percent_Identity=51.6528925619835, Blast_Score=258, Evalue=2e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_ACIAD (Q43963)

Other databases:

- EMBL:   CR543861
- EMBL:   X80800
- PIR:   S61928
- RefSeq:   YP_047806.1
- ProteinModelPortal:   Q43963
- SMR:   Q43963
- STRING:   Q43963
- GeneID:   2880730
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD3311
- NMPDR:   fig|62977.3.peg.2999
- eggNOG:   COG0336
- HOGENOM:   HBG285805
- OMA:   VCGRFEG
- PhylomeDB:   Q43963
- ProtClustDB:   PRK00026
- BioCyc:   ASP62977:ACIAD3311-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 28288; Mature: 28288

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: NA

Important sites: BINDING 117-117

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFFAVITLFPEMFEAITAYGISGRATKRQIATVSCINPRDFAEGNYKRVDERPFGGGPGM
CHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHEEEECCCHHHCCCCCCCCCCCCCCCCCCC
VMMAEPLAKAIIHAKQLAEQAGCVHAPVVYMSPQGKTLNEHAVQQFVDYDGLIVLCGRYE
EEHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCEEEEECCCC
GVDERLIQHYVDQEWSIGDYVLSGGELPAMVLLDSIIRRLPDAMSDEQSHIQDSFVDGLL
CHHHHHHHHHHCCCCCCCHHEECCCCCHHHHHHHHHHHHCCHHCCCHHHHHHHHHHHHHH
DCPQYTKPDHFEGLDVPEVLKSGHHANIEKWRFLQRYQRTLDRRPELVEKVTLTKQQRKW
CCCCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
LTFLDDSKN
HHHCCCCCC
>Mature Secondary Structure
MFFAVITLFPEMFEAITAYGISGRATKRQIATVSCINPRDFAEGNYKRVDERPFGGGPGM
CHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHEEEECCCHHHCCCCCCCCCCCCCCCCCCC
VMMAEPLAKAIIHAKQLAEQAGCVHAPVVYMSPQGKTLNEHAVQQFVDYDGLIVLCGRYE
EEHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCEEEEECCCC
GVDERLIQHYVDQEWSIGDYVLSGGELPAMVLLDSIIRRLPDAMSDEQSHIQDSFVDGLL
CHHHHHHHHHHCCCCCCCHHEECCCCCHHHHHHHHHHHHCCHHCCCHHHHHHHHHHHHHH
DCPQYTKPDHFEGLDVPEVLKSGHHANIEKWRFLQRYQRTLDRRPELVEKVTLTKQQRKW
CCCCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
LTFLDDSKN
HHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7768830