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The map label for this gene is recG [H]

Identifier: 50086230

GI number: 50086230

Start: 3150747

End: 3152807

Strand: Direct

Name: recG [H]

Synonym: ACIAD3235

Alternate gene names: 50086230

Gene position: 3150747-3152807 (Clockwise)

Preceding gene: 50086227

Following gene: 50086231

Centisome position: 87.55

GC content: 44.74

Gene sequence:

>2061_bases
CTGATTTGGGGGGACATGACTTCAGTCCATCATTTGCAAGGCGTTGGAACTGCCGCAGCCAGCTTGCTCGAAAAACTTAA
TATCTTTACCACAGATGATTTGTTGTTTCATTTACCACGCGATTATGAAGATCGTAGCACGGTTATTGCAATGAACCAAC
TGATGGTTGGGCGAAGCTATTTGCTTGAAGGTGAAATAAAATCACTCGATTTCCCAGCAGGTAAACGCAAATCTGTAGCG
GCCTTACTGCAAGATGATTGCGGAAAAGTCACCTTACGCTTTTATCATATTTATAAGGGTTTGACCGATAAACTTAAATC
TGGGCAACGGCTACGGGTGTTTGGTGAGGTTCGTGTTGGTGCACGTGGCTTAGAAATGTATCATCCTGAAATTCAGGTTA
TTCACGACTATACGCCACTTCCTAAAACCCAGCTCACGGCCATCTACCCAAGTACCGAAGGTTTAACCCAACCCAAATTA
CGCGAATATATCAAGCAAGCGCTCAAACAGTATAGCGATAACCTACCTGAATTACTCCCCAAACAATTTACCAATGGTTA
TGTCTTAAAAGAAGCGCTGCACTATATTCATGAGCCACCGCTAAATGCCAATATGCAACAACTGGCTTTAGGTTCACATC
CTGCTCAGCAACGTCTGATTTTTGAGGAACTGGTCGCACATCAGATTAGCTTACTTACTCGACGTGCTTATATTCGTCAG
ATTGCCTCACCAAAATTTGAACCCAGCCAAACTTTAGCAAAAGCATTACTGGCACAGCTTCCTTTTCAGATGACAAGCGC
TCAAAAACGTGTTTCAAAAGAAATTGCGCATGATCTTCATCAATCACAACCTATGCTCAGATTGGTACAAGGCGATGTAG
GTGCGGGCAAAACCTTAGTCGCTGCAATAGCCGCCTGTCATGCGCTGGAAGCTGGCTGGCAAGTCGCATTGATGGCACCT
ACCGAAATTTTGGCCGAACAGCATTATCTTAATTTTAAACGCTGGTTTGAACCTTTAAGCATTCAGGTCGGCTGGCTTTC
TGGCAAGCAAAAAGGTAAAGCTCGCACTCAAGCCGAAGAACTGATCCGAACTGGACAAGTCCAACTGGTCATGGGTACGC
ATGCACTCTTTCAGGATAACGTCGAGTTTGCCAAGCTGGGGCTGGTCATTATTGATGAACAGCATCGTTTTGGTGTTGAT
CAACGCTTGGCTCTTCGCAATAAAGGCGTAGACCACTTCACCCCACACCAGTTGGTAATGACAGCAACTCCCATCCCACG
AACACTTGCAATGAGTGCCTATGGTGATTTAGATACTTCAGTGATTGATGAATTACCCCCTGGCCGAACGCCAATTCAAA
CGGTGACGATTCCACTAGATCGGCGCGAAGAAGTCATCAATAGAATTGCAAGCAATTGCCTTGAAGGTAAACAAGCTTAT
TGGGTATGTACTTTGGTTGAACAGTCCGAAACGCTGGATGCTCAAGCCGCAGAGGCCACCTATCAGGAAATACGCTCACG
TTTTCCAGAACTTAAAGTTGGACTTGTACACGGCAAAATGAAAGCCGAAGAAAAACAGAATGTCATGCAAGCCTTTAAAG
ACAACCAGCTACAACTCTTAATTGCCACAACGGTAATTGAAGTAGGTGTTGATGTACCCAATGCATCAATCATGGTCATT
GAAAATGCTGAACGATTAGGGCTATCTCAATTACATCAACTGCGGGGTCGGGTGGGTCGTGGTGCCAAAGCCAGCTTTTG
TGTGCTGCTGTATAAGCATCCACTGTCACAAAATGGGCAAGAGCGTCTTTCTATATTAAGAGAAAGTAACGATGGCTTTG
TCATTGCCGAAAAAGATTTAGAGTTACGTGGCCCAGGTGAATTATTAGGAACCAAGCAAACAGGTGATATGAATTTTCGT
GTTGCAAAGTTAGAACGTGATGATCATCTATTGGTGCAAGCACATTATGTCGCCGAGCAAGTGTTAAAAGATCATGCTCA
ACAGGCAGAGGCTTTATTAAACCGCTGGCTGCCAGACGCCCCCCGATATGCTTATGTCTAA

Upstream 100 bases:

>100_bases
CGGCTTACCGCAGTAATTTGATGGCCTTGTGCAAATAAATGTTTAGCAATACGCTGAGATGTTTTACCATAGCCAATAAA
TAAAATATGCATCGGACATC

Downstream 100 bases:

>100_bases
TTTGCTTGCAACCACACTTCAATGGCTTAAAAAAGAGTGCGTCCCCTGCCCATTATGTGAATTAAGTCCGCAGCAAAAAC
AGCAGATACTCTGTAAGGAT

Product: ATP-dependent DNA helicase RecG

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 686; Mature: 686

Protein sequence:

>686_residues
MIWGDMTSVHHLQGVGTAAASLLEKLNIFTTDDLLFHLPRDYEDRSTVIAMNQLMVGRSYLLEGEIKSLDFPAGKRKSVA
ALLQDDCGKVTLRFYHIYKGLTDKLKSGQRLRVFGEVRVGARGLEMYHPEIQVIHDYTPLPKTQLTAIYPSTEGLTQPKL
REYIKQALKQYSDNLPELLPKQFTNGYVLKEALHYIHEPPLNANMQQLALGSHPAQQRLIFEELVAHQISLLTRRAYIRQ
IASPKFEPSQTLAKALLAQLPFQMTSAQKRVSKEIAHDLHQSQPMLRLVQGDVGAGKTLVAAIAACHALEAGWQVALMAP
TEILAEQHYLNFKRWFEPLSIQVGWLSGKQKGKARTQAEELIRTGQVQLVMGTHALFQDNVEFAKLGLVIIDEQHRFGVD
QRLALRNKGVDHFTPHQLVMTATPIPRTLAMSAYGDLDTSVIDELPPGRTPIQTVTIPLDRREEVINRIASNCLEGKQAY
WVCTLVEQSETLDAQAAEATYQEIRSRFPELKVGLVHGKMKAEEKQNVMQAFKDNQLQLLIATTVIEVGVDVPNASIMVI
ENAERLGLSQLHQLRGRVGRGAKASFCVLLYKHPLSQNGQERLSILRESNDGFVIAEKDLELRGPGELLGTKQTGDMNFR
VAKLERDDHLLVQAHYVAEQVLKDHAQQAEALLNRWLPDAPRYAYV

Sequences:

>Translated_686_residues
MIWGDMTSVHHLQGVGTAAASLLEKLNIFTTDDLLFHLPRDYEDRSTVIAMNQLMVGRSYLLEGEIKSLDFPAGKRKSVA
ALLQDDCGKVTLRFYHIYKGLTDKLKSGQRLRVFGEVRVGARGLEMYHPEIQVIHDYTPLPKTQLTAIYPSTEGLTQPKL
REYIKQALKQYSDNLPELLPKQFTNGYVLKEALHYIHEPPLNANMQQLALGSHPAQQRLIFEELVAHQISLLTRRAYIRQ
IASPKFEPSQTLAKALLAQLPFQMTSAQKRVSKEIAHDLHQSQPMLRLVQGDVGAGKTLVAAIAACHALEAGWQVALMAP
TEILAEQHYLNFKRWFEPLSIQVGWLSGKQKGKARTQAEELIRTGQVQLVMGTHALFQDNVEFAKLGLVIIDEQHRFGVD
QRLALRNKGVDHFTPHQLVMTATPIPRTLAMSAYGDLDTSVIDELPPGRTPIQTVTIPLDRREEVINRIASNCLEGKQAY
WVCTLVEQSETLDAQAAEATYQEIRSRFPELKVGLVHGKMKAEEKQNVMQAFKDNQLQLLIATTVIEVGVDVPNASIMVI
ENAERLGLSQLHQLRGRVGRGAKASFCVLLYKHPLSQNGQERLSILRESNDGFVIAEKDLELRGPGELLGTKQTGDMNFR
VAKLERDDHLLVQAHYVAEQVLKDHAQQAEALLNRWLPDAPRYAYV
>Mature_686_residues
MIWGDMTSVHHLQGVGTAAASLLEKLNIFTTDDLLFHLPRDYEDRSTVIAMNQLMVGRSYLLEGEIKSLDFPAGKRKSVA
ALLQDDCGKVTLRFYHIYKGLTDKLKSGQRLRVFGEVRVGARGLEMYHPEIQVIHDYTPLPKTQLTAIYPSTEGLTQPKL
REYIKQALKQYSDNLPELLPKQFTNGYVLKEALHYIHEPPLNANMQQLALGSHPAQQRLIFEELVAHQISLLTRRAYIRQ
IASPKFEPSQTLAKALLAQLPFQMTSAQKRVSKEIAHDLHQSQPMLRLVQGDVGAGKTLVAAIAACHALEAGWQVALMAP
TEILAEQHYLNFKRWFEPLSIQVGWLSGKQKGKARTQAEELIRTGQVQLVMGTHALFQDNVEFAKLGLVIIDEQHRFGVD
QRLALRNKGVDHFTPHQLVMTATPIPRTLAMSAYGDLDTSVIDELPPGRTPIQTVTIPLDRREEVINRIASNCLEGKQAY
WVCTLVEQSETLDAQAAEATYQEIRSRFPELKVGLVHGKMKAEEKQNVMQAFKDNQLQLLIATTVIEVGVDVPNASIMVI
ENAERLGLSQLHQLRGRVGRGAKASFCVLLYKHPLSQNGQERLSILRESNDGFVIAEKDLELRGPGELLGTKQTGDMNFR
VAKLERDDHLLVQAHYVAEQVLKDHAQQAEALLNRWLPDAPRYAYV

Specific function: Critical role in recombination and DNA repair. Help process Holliday junction intermediates to mature products by catalyzing branch migration. Has a DNA unwinding activity characteristic of a DNA helicase with a 3' to 5' polarity. RecG unwind branched dup

COG id: COG1200

COG function: function code LK; RecG-like helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367254, Length=685, Percent_Identity=54.8905109489051, Blast_Score=727, Evalue=0.0,
Organism=Escherichia coli, GI1787357, Length=388, Percent_Identity=38.4020618556701, Blast_Score=252, Evalue=5e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR004609
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C; PF01336 tRNA_anti [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 77092; Mature: 77092

Theoretical pI: Translated: 7.81; Mature: 7.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIWGDMTSVHHLQGVGTAAASLLEKLNIFTTDDLLFHLPRDYEDRSTVIAMNQLMVGRSY
CCCCCCCHHHHHHCCHHHHHHHHHHHCCCEECHHHEECCCCCCCCHHEEEHHHHHHCHHH
LLEGEIKSLDFPAGKRKSVAALLQDDCGKVTLRFYHIYKGLTDKLKSGQRLRVFGEVRVG
EECCCCCCCCCCCCCHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHCCCCEEEEEEEEECC
ARGLEMYHPEIQVIHDYTPLPKTQLTAIYPSTEGLTQPKLREYIKQALKQYSDNLPELLP
CCCCEEECCCEEEEECCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCHHHHH
KQFTNGYVLKEALHYIHEPPLNANMQQLALGSHPAQQRLIFEELVAHQISLLTRRAYIRQ
HHHCCCHHHHHHHHHHCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
IASPKFEPSQTLAKALLAQLPFQMTSAQKRVSKEIAHDLHQSQPMLRLVQGDVGAGKTLV
HCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCHHHH
AAIAACHALEAGWQVALMAPTEILAEQHYLNFKRWFEPLSIQVGWLSGKQKGKARTQAEE
HHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHH
LIRTGQVQLVMGTHALFQDNVEFAKLGLVIIDEQHRFGVDQRLALRNKGVDHFTPHQLVM
HHHCCCEEEEECCHHHHHCCCCEEHEEEEEEECCHHCCCHHHHHHHHCCCCCCCCCEEEE
TATPIPRTLAMSAYGDLDTSVIDELPPGRTPIQTVTIPLDRREEVINRIASNCLEGKQAY
ECCCCCHHHHHHHCCCCCHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHCCCCEE
WVCTLVEQSETLDAQAAEATYQEIRSRFPELKVGLVHGKMKAEEKQNVMQAFKDNQLQLL
EEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHCCCCEEEE
IATTVIEVGVDVPNASIMVIENAERLGLSQLHQLRGRVGRGAKASFCVLLYKHPLSQNGQ
EEHHHHHHCCCCCCCEEEEEECHHHCCHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCHH
ERLSILRESNDGFVIAEKDLELRGPGELLGTKQTGDMNFRVAKLERDDHLLVQAHYVAEQ
HHHHHHHCCCCCEEEEECCCCCCCCHHHCCCCCCCCCCEEEEEECCCCCEEEHHHHHHHH
VLKDHAQQAEALLNRWLPDAPRYAYV
HHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MIWGDMTSVHHLQGVGTAAASLLEKLNIFTTDDLLFHLPRDYEDRSTVIAMNQLMVGRSY
CCCCCCCHHHHHHCCHHHHHHHHHHHCCCEECHHHEECCCCCCCCHHEEEHHHHHHCHHH
LLEGEIKSLDFPAGKRKSVAALLQDDCGKVTLRFYHIYKGLTDKLKSGQRLRVFGEVRVG
EECCCCCCCCCCCCCHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHCCCCEEEEEEEEECC
ARGLEMYHPEIQVIHDYTPLPKTQLTAIYPSTEGLTQPKLREYIKQALKQYSDNLPELLP
CCCCEEECCCEEEEECCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCHHHHH
KQFTNGYVLKEALHYIHEPPLNANMQQLALGSHPAQQRLIFEELVAHQISLLTRRAYIRQ
HHHCCCHHHHHHHHHHCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
IASPKFEPSQTLAKALLAQLPFQMTSAQKRVSKEIAHDLHQSQPMLRLVQGDVGAGKTLV
HCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCHHHH
AAIAACHALEAGWQVALMAPTEILAEQHYLNFKRWFEPLSIQVGWLSGKQKGKARTQAEE
HHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHH
LIRTGQVQLVMGTHALFQDNVEFAKLGLVIIDEQHRFGVDQRLALRNKGVDHFTPHQLVM
HHHCCCEEEEECCHHHHHCCCCEEHEEEEEEECCHHCCCHHHHHHHHCCCCCCCCCEEEE
TATPIPRTLAMSAYGDLDTSVIDELPPGRTPIQTVTIPLDRREEVINRIASNCLEGKQAY
ECCCCCHHHHHHHCCCCCHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHCCCCEE
WVCTLVEQSETLDAQAAEATYQEIRSRFPELKVGLVHGKMKAEEKQNVMQAFKDNQLQLL
EEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHCCCCEEEE
IATTVIEVGVDVPNASIMVIENAERLGLSQLHQLRGRVGRGAKASFCVLLYKHPLSQNGQ
EEHHHHHHCCCCCCCEEEEEECHHHCCHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCHH
ERLSILRESNDGFVIAEKDLELRGPGELLGTKQTGDMNFRVAKLERDDHLLVQAHYVAEQ
HHHHHHHCCCCCEEEEECCCCCCCCHHHCCCCCCCCCCEEEEEECCCCCEEEHHHHHHHH
VLKDHAQQAEALLNRWLPDAPRYAYV
HHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]