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The map label for this gene is plsB [H]

Identifier: 50086227

GI number: 50086227

Start: 3146193

End: 3148757

Strand: Direct

Name: plsB [H]

Synonym: ACIAD3232

Alternate gene names: 50086227

Gene position: 3146193-3148757 (Clockwise)

Preceding gene: 50086224

Following gene: 50086230

Centisome position: 87.43

GC content: 43.31

Gene sequence:

>2565_bases
ATGTCCCAGCGTGGCTTCGGTCAGATGTATCGACGGCTTTCCAGTAAGTTACTTGACCTTGTGGTAACTCCACATGTTCT
TGGGGAAATCCCTACTGAACCAGCACAGCATTCAAATGCTACAACACCAGATTCAAAAAAAATCGTGTGCTATGTTTTGC
AGAATTACTCGCGTAGTAATGCCTTGATCGTTGACAGTGAAACTCGTCGTCTCGATTTAAAACCTGCACTTGATCCTTTG
GTTATAGCTGAGCATCAGGAAAAAGCGTCTGTTTTATTTTTGCAGCATCACGATGAGCGTAATCTGCTTAATCCGCCACC
TCATGCTTTTCCGCCTCGTTTGTTGCGATTGATTGAAGTTTTGGAAAAGCATCCTGAATATGATATCGAGCTGGTTCCCG
TGACGGTTCTTTGGGGACGTGCACCAGATAAAGAAGACTCATGGGTTAAGCTTTTATTTACTGATACTTGGGCAACACCA
AGCAAGATCAAGCAGTTGGTCAATATCGGTTTACATGGTCGTCAGTCCTATCTTGAGTTTCATGAGCCACAGTCCTTGCG
TCATTTGGTCGATTATGCCAAAACGCATTATCCGAATTTGTCACCAGCGACCTATATTATTAGTACGCTCAATGATTATC
TGGATCGTCAACGTGAAGTCGTATTGGGCCCAGATTTATCTGACCGTCGCAATGTGATGGAATCATTGATCAAGTCACGC
GATGTTCAGGATGCAATTCGTCGTGAAAGTATTCGTGGCAAAATCAGTATGCTGGAAGCCGAGCGCCGCGCGATTGGTTA
TTTAAACGAAATCGTGTCGGACTATTCGCATTCTGCCGTACGTTTTGCCGATATGGCACTGACTCGTTTATGGACACAGC
TCTATGATGGTGTTGAAGTTCATAATTTTAGTACAGTGCGCGAACTGGCCAAAGATTACGAGATTATTTATACCCCGTGT
CATCGCAGTCATATCGACTATTTGCTGTTGTCCTATGTCATTTACAAACGCGGCTTGATGGTTCCGTACATTGCAGCGGG
TGACAATCTTAACTTGCCATTCGTTGGTCAGCTATTGCGTGGTGGTGGTGCATTCTTCATTCGACGTTCTTTCCGTGGTA
ATGGCTTATATACTTCGGTTTTTAAAGAATATCTATACAGTATTTTGTCACGTAACACGCCGCTTGAATATTTCATTGAA
GGCGGACGTTCACGAACTGGTCGTTTACTTCCACCTAAAACTGGCATGCTGGCCATGACCATTCACGGTCATTTACGTGG
TCGTGCTAAACCAATTGTGTTTTTACCGACTTATATCGGTTATGAACGCCTCATGGAAGGTTCAACTTACGTAGGTGAAA
TGCAAGGTAAACCCAAAGAAGCTGAATCAGTGGTAGGAATCTTAAAAACCTTACGCAAGATTGAGCGAATTTTTGGAAAG
GTCCATGTTAATTTCGGTGAGCCTGTTTTTTTAGATGACATGCTTAAGGCACATGGTGCCGACCAGATGAAAATCGAGAA
AAATGACGATCCACTGCCAAATGAAGTTTCATTGGCAATTAATAGCTCGGCACACGCAATTCTGGAAAATATCAACCGCG
CAGTGGTGATTAATCCTGTGTCGTTGCTCTCTTTGATTCTATTGGCAACCTCCAAGCATACGCTGGATGAAGAACTTTGC
ATCAAACAACTGGACGCATATCGCACGCTTATGGCGAAACAGCCTTATGATGAGCGTAGTCAGGTCACCTCTCTTTCAGG
CAAAGAAATCATTGCTTACGGTTTGAAGTTAAAACTGATCAAACGGGTTCAGCACGCGCTTGGTGATATTATTGCAATTG
AAGACAATCAGGCTGTTTTACTGACCTACTTCCGTAACAATATCCTGCATGCATTTGTATTGCCATCTCTGGTGGCCTCA
CTGGTTGAACATAATGGCAAGATCACCCGAAAAGATCTGCATAATGTCATCCGAACGCTGTATCCATTTTTACAAGCCGA
ATTATTCCTAAAATGGAAGCATGAAGCGCTTAATCAACACATTGATCGTTATGTCGATGCATTGGTTGAATCTGGCTGGA
TTGAGCTGGATCAAGATGGAGACTTAGTGAGCCTAGCGCCAAATTCTGAAGAGCATAATCAGCTTGTAATTCTGGCTGCA
CCTGTAAAACAGAGTCTTGAGCGTTATTACATGACCTTGGCACTTATTGCACAGCGTGGTTCAGGTAATATTTCCGCCAA
GCAGGTTGAAGACCTCAGTCATTTGCTGGGACAACGTCTATCTGTTCTTTACGAATTTAACTCTCCTGAATTTTTTGATA
AAGCACTCTTTCAAAGTTTCTTGAAAGTTCTCACGCAACAAGGTTATATCCGAACCAACAGTGAAGGCTCAATTGAGTTT
GATCGTCAGTTTAGCAATATGGCACAAGGTGCCAAACTGGTGCTTGATGAAGCGACGCTCCAGATGTTGCAGCACATTAC
TACATTTAGTGATGACGAACTCAAAGCTGCACTTGAGGCCATGGCTTCACAACAAGCGAAACGTCGCTTACGCAAGAAAA
AATAA

Upstream 100 bases:

>100_bases
ATTTAGCAGAAAATAGCGCATCGTTTTTGCAAATCAATTCATCAAAAGATACACGTCTTTATTTTCAGCAGTGTGTCTGG
TTGTTTGAGTAGGAGATTTT

Downstream 100 bases:

>100_bases
TTTTTAAATCACTACTCAATAAAGGTCACCACGGTGACCTTTATTTATGAGCGAAACTGGCACTCAAAATAACGCGGTTC
ATCCATACAGCGTAAACGGC

Product: glycerol-3-phosphate acyltransferase

Products: NA

Alternate protein names: GPAT [H]

Number of amino acids: Translated: 854; Mature: 853

Protein sequence:

>854_residues
MSQRGFGQMYRRLSSKLLDLVVTPHVLGEIPTEPAQHSNATTPDSKKIVCYVLQNYSRSNALIVDSETRRLDLKPALDPL
VIAEHQEKASVLFLQHHDERNLLNPPPHAFPPRLLRLIEVLEKHPEYDIELVPVTVLWGRAPDKEDSWVKLLFTDTWATP
SKIKQLVNIGLHGRQSYLEFHEPQSLRHLVDYAKTHYPNLSPATYIISTLNDYLDRQREVVLGPDLSDRRNVMESLIKSR
DVQDAIRRESIRGKISMLEAERRAIGYLNEIVSDYSHSAVRFADMALTRLWTQLYDGVEVHNFSTVRELAKDYEIIYTPC
HRSHIDYLLLSYVIYKRGLMVPYIAAGDNLNLPFVGQLLRGGGAFFIRRSFRGNGLYTSVFKEYLYSILSRNTPLEYFIE
GGRSRTGRLLPPKTGMLAMTIHGHLRGRAKPIVFLPTYIGYERLMEGSTYVGEMQGKPKEAESVVGILKTLRKIERIFGK
VHVNFGEPVFLDDMLKAHGADQMKIEKNDDPLPNEVSLAINSSAHAILENINRAVVINPVSLLSLILLATSKHTLDEELC
IKQLDAYRTLMAKQPYDERSQVTSLSGKEIIAYGLKLKLIKRVQHALGDIIAIEDNQAVLLTYFRNNILHAFVLPSLVAS
LVEHNGKITRKDLHNVIRTLYPFLQAELFLKWKHEALNQHIDRYVDALVESGWIELDQDGDLVSLAPNSEEHNQLVILAA
PVKQSLERYYMTLALIAQRGSGNISAKQVEDLSHLLGQRLSVLYEFNSPEFFDKALFQSFLKVLTQQGYIRTNSEGSIEF
DRQFSNMAQGAKLVLDEATLQMLQHITTFSDDELKAALEAMASQQAKRRLRKKK

Sequences:

>Translated_854_residues
MSQRGFGQMYRRLSSKLLDLVVTPHVLGEIPTEPAQHSNATTPDSKKIVCYVLQNYSRSNALIVDSETRRLDLKPALDPL
VIAEHQEKASVLFLQHHDERNLLNPPPHAFPPRLLRLIEVLEKHPEYDIELVPVTVLWGRAPDKEDSWVKLLFTDTWATP
SKIKQLVNIGLHGRQSYLEFHEPQSLRHLVDYAKTHYPNLSPATYIISTLNDYLDRQREVVLGPDLSDRRNVMESLIKSR
DVQDAIRRESIRGKISMLEAERRAIGYLNEIVSDYSHSAVRFADMALTRLWTQLYDGVEVHNFSTVRELAKDYEIIYTPC
HRSHIDYLLLSYVIYKRGLMVPYIAAGDNLNLPFVGQLLRGGGAFFIRRSFRGNGLYTSVFKEYLYSILSRNTPLEYFIE
GGRSRTGRLLPPKTGMLAMTIHGHLRGRAKPIVFLPTYIGYERLMEGSTYVGEMQGKPKEAESVVGILKTLRKIERIFGK
VHVNFGEPVFLDDMLKAHGADQMKIEKNDDPLPNEVSLAINSSAHAILENINRAVVINPVSLLSLILLATSKHTLDEELC
IKQLDAYRTLMAKQPYDERSQVTSLSGKEIIAYGLKLKLIKRVQHALGDIIAIEDNQAVLLTYFRNNILHAFVLPSLVAS
LVEHNGKITRKDLHNVIRTLYPFLQAELFLKWKHEALNQHIDRYVDALVESGWIELDQDGDLVSLAPNSEEHNQLVILAA
PVKQSLERYYMTLALIAQRGSGNISAKQVEDLSHLLGQRLSVLYEFNSPEFFDKALFQSFLKVLTQQGYIRTNSEGSIEF
DRQFSNMAQGAKLVLDEATLQMLQHITTFSDDELKAALEAMASQQAKRRLRKKK
>Mature_853_residues
SQRGFGQMYRRLSSKLLDLVVTPHVLGEIPTEPAQHSNATTPDSKKIVCYVLQNYSRSNALIVDSETRRLDLKPALDPLV
IAEHQEKASVLFLQHHDERNLLNPPPHAFPPRLLRLIEVLEKHPEYDIELVPVTVLWGRAPDKEDSWVKLLFTDTWATPS
KIKQLVNIGLHGRQSYLEFHEPQSLRHLVDYAKTHYPNLSPATYIISTLNDYLDRQREVVLGPDLSDRRNVMESLIKSRD
VQDAIRRESIRGKISMLEAERRAIGYLNEIVSDYSHSAVRFADMALTRLWTQLYDGVEVHNFSTVRELAKDYEIIYTPCH
RSHIDYLLLSYVIYKRGLMVPYIAAGDNLNLPFVGQLLRGGGAFFIRRSFRGNGLYTSVFKEYLYSILSRNTPLEYFIEG
GRSRTGRLLPPKTGMLAMTIHGHLRGRAKPIVFLPTYIGYERLMEGSTYVGEMQGKPKEAESVVGILKTLRKIERIFGKV
HVNFGEPVFLDDMLKAHGADQMKIEKNDDPLPNEVSLAINSSAHAILENINRAVVINPVSLLSLILLATSKHTLDEELCI
KQLDAYRTLMAKQPYDERSQVTSLSGKEIIAYGLKLKLIKRVQHALGDIIAIEDNQAVLLTYFRNNILHAFVLPSLVASL
VEHNGKITRKDLHNVIRTLYPFLQAELFLKWKHEALNQHIDRYVDALVESGWIELDQDGDLVSLAPNSEEHNQLVILAAP
VKQSLERYYMTLALIAQRGSGNISAKQVEDLSHLLGQRLSVLYEFNSPEFFDKALFQSFLKVLTQQGYIRTNSEGSIEFD
RQFSNMAQGAKLVLDEATLQMLQHITTFSDDELKAALEAMASQQAKRRLRKKK

Specific function: De novo phospholipid biosynthesis; first step. [C]

COG id: COG2937

COG function: function code I; Glycerol-3-phosphate O-acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GPAT/DAPAT family [H]

Homologues:

Organism=Homo sapiens, GI7657134, Length=422, Percent_Identity=29.3838862559242, Blast_Score=182, Evalue=8e-46,
Organism=Homo sapiens, GI190358539, Length=472, Percent_Identity=26.6949152542373, Blast_Score=141, Evalue=2e-33,
Organism=Escherichia coli, GI87082362, Length=794, Percent_Identity=37.4055415617128, Blast_Score=475, Evalue=1e-135,
Organism=Caenorhabditis elegans, GI25147672, Length=355, Percent_Identity=30.1408450704225, Blast_Score=167, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI71988723, Length=253, Percent_Identity=33.596837944664, Blast_Score=144, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI71988728, Length=190, Percent_Identity=37.8947368421053, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI17864692, Length=311, Percent_Identity=34.4051446945338, Blast_Score=180, Evalue=4e-45,
Organism=Drosophila melanogaster, GI24650754, Length=426, Percent_Identity=26.7605633802817, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24650752, Length=426, Percent_Identity=26.7605633802817, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI21357731, Length=426, Percent_Identity=26.7605633802817, Blast_Score=147, Evalue=3e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002123
- InterPro:   IPR022284 [H]

Pfam domain/function: PF01553 Acyltransferase [H]

EC number: =2.3.1.15 [H]

Molecular weight: Translated: 97277; Mature: 97146

Theoretical pI: Translated: 8.36; Mature: 8.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQRGFGQMYRRLSSKLLDLVVTPHVLGEIPTEPAQHSNATTPDSKKIVCYVLQNYSRSN
CCCCCHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCC
ALIVDSETRRLDLKPALDPLVIAEHQEKASVLFLQHHDERNLLNPPPHAFPPRLLRLIEV
EEEEECCCCEEECCCCCCCEEEECCCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHH
LEKHPEYDIELVPVTVLWGRAPDKEDSWVKLLFTDTWATPSKIKQLVNIGLHGRQSYLEF
HHHCCCCCEEEEEEEEEECCCCCCCCCEEEEEEECCCCCHHHHHHHHHCCCCCCHHHHHH
HEPQSLRHLVDYAKTHYPNLSPATYIISTLNDYLDRQREVVLGPDLSDRRNVMESLIKSR
CCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHCC
DVQDAIRRESIRGKISMLEAERRAIGYLNEIVSDYSHSAVRFADMALTRLWTQLYDGVEV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCEE
HNFSTVRELAKDYEIIYTPCHRSHIDYLLLSYVIYKRGLMVPYIAAGDNLNLPFVGQLLR
CCHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHH
GGGAFFIRRSFRGNGLYTSVFKEYLYSILSRNTPLEYFIEGGRSRTGRLLPPKTGMLAMT
CCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCHHHHEECCCCCCCCCCCCCCCEEEEE
IHGHLRGRAKPIVFLPTYIGYERLMEGSTYVGEMQGKPKEAESVVGILKTLRKIERIFGK
EEHHCCCCCCCEEEECHHHCHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHE
VHVNFGEPVFLDDMLKAHGADQMKIEKNDDPLPNEVSLAINSSAHAILENINRAVVINPV
EEECCCCCCHHHHHHHHCCCCCEEEECCCCCCCCCEEEEECCHHHHHHHCCCCEEEECHH
SLLSLILLATSKHTLDEELCIKQLDAYRTLMAKQPYDERSQVTSLSGKEIIAYGLKLKLI
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCHHHHHHHHHHHH
KRVQHALGDIIAIEDNQAVLLTYFRNNILHAFVLPSLVASLVEHNGKITRKDLHNVIRTL
HHHHHHHCCEEEEECCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
YPFLQAELFLKWKHEALNQHIDRYVDALVESGWIELDQDGDLVSLAPNSEEHNQLVILAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCEEEECCCCCCCCEEEEEEC
PVKQSLERYYMTLALIAQRGSGNISAKQVEDLSHLLGQRLSVLYEFNSPEFFDKALFQSF
CHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHH
LKVLTQQGYIRTNSEGSIEFDRQFSNMAQGAKLVLDEATLQMLQHITTFSDDELKAALEA
HHHHHHCCCEEECCCCCEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
MASQQAKRRLRKKK
HHHHHHHHHHHCCC
>Mature Secondary Structure 
SQRGFGQMYRRLSSKLLDLVVTPHVLGEIPTEPAQHSNATTPDSKKIVCYVLQNYSRSN
CCCCHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCC
ALIVDSETRRLDLKPALDPLVIAEHQEKASVLFLQHHDERNLLNPPPHAFPPRLLRLIEV
EEEEECCCCEEECCCCCCCEEEECCCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHH
LEKHPEYDIELVPVTVLWGRAPDKEDSWVKLLFTDTWATPSKIKQLVNIGLHGRQSYLEF
HHHCCCCCEEEEEEEEEECCCCCCCCCEEEEEEECCCCCHHHHHHHHHCCCCCCHHHHHH
HEPQSLRHLVDYAKTHYPNLSPATYIISTLNDYLDRQREVVLGPDLSDRRNVMESLIKSR
CCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHCC
DVQDAIRRESIRGKISMLEAERRAIGYLNEIVSDYSHSAVRFADMALTRLWTQLYDGVEV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCEE
HNFSTVRELAKDYEIIYTPCHRSHIDYLLLSYVIYKRGLMVPYIAAGDNLNLPFVGQLLR
CCHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHH
GGGAFFIRRSFRGNGLYTSVFKEYLYSILSRNTPLEYFIEGGRSRTGRLLPPKTGMLAMT
CCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCHHHHEECCCCCCCCCCCCCCCEEEEE
IHGHLRGRAKPIVFLPTYIGYERLMEGSTYVGEMQGKPKEAESVVGILKTLRKIERIFGK
EEHHCCCCCCCEEEECHHHCHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHE
VHVNFGEPVFLDDMLKAHGADQMKIEKNDDPLPNEVSLAINSSAHAILENINRAVVINPV
EEECCCCCCHHHHHHHHCCCCCEEEECCCCCCCCCEEEEECCHHHHHHHCCCCEEEECHH
SLLSLILLATSKHTLDEELCIKQLDAYRTLMAKQPYDERSQVTSLSGKEIIAYGLKLKLI
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCHHHHHHHHHHHH
KRVQHALGDIIAIEDNQAVLLTYFRNNILHAFVLPSLVASLVEHNGKITRKDLHNVIRTL
HHHHHHHCCEEEEECCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
YPFLQAELFLKWKHEALNQHIDRYVDALVESGWIELDQDGDLVSLAPNSEEHNQLVILAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCEEEECCCCCCCCEEEEEEC
PVKQSLERYYMTLALIAQRGSGNISAKQVEDLSHLLGQRLSVLYEFNSPEFFDKALFQSF
CHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHH
LKVLTQQGYIRTNSEGSIEFDRQFSNMAQGAKLVLDEATLQMLQHITTFSDDELKAALEA
HHHHHHCCCEEECCCCCEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
MASQQAKRRLRKKK
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA