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The map label for this gene is alsR [H]

Identifier: 50086170

GI number: 50086170

Start: 3097068

End: 3097979

Strand: Reverse

Name: alsR [H]

Synonym: ACIAD3169

Alternate gene names: 50086170

Gene position: 3097979-3097068 (Counterclockwise)

Preceding gene: 50086172

Following gene: 50086168

Centisome position: 86.09

GC content: 42.0

Gene sequence:

>912_bases
ATGGAATTACGACATATCAGATATTTTCTGGTGGTAGCAGAAGAAAAAAGCTTTACCAGAGCAGCAGCTCGCCTTGGCAT
CAGTCAGCCGCCTTTGAGCATGCAGATCAAAAATCTTGAGGACGAGATTGGAACCGAGTTGTTTTATCGTTCAGCCCAAG
GTACAGAACTCACACCAGCAGGAAAAGCTTTTCTGGTGAGTGTGCAACCGATTCAGGAGCGTATTGCAGAAGCGGTACAG
CTTGCACAACAAGTGGCCAATGGTGAGTCGGGACAGCTCCGTTTGGGATTTACAGGCACATCCATGCTCAATCTGATTTT
GCCCAAAAGTATTCGGCTTTTTCAACATCAATACCCCAAAATTAGACTTAAACTTGAGGAAGCAAATTCATTACTGTTAA
TGGATCGATTACTTGATGATCGGCTGGATATTGCATTGGTTCGTCCGCCAGATCATGTACCTGAAGGATTACATTTGCAA
TTATTGATGCACGAACCTTTACTTGCTGTTTTACCGATTGGGCATCCATTTGCGCAGCATCACTCTATTCAGTTAAATGC
GTTGAAAGATGATAGTTTTATTGTCTCTCCACGAGCCATCAGTGCAGGATTATTTGATGCAATTGCCAATGCGTGTCGTA
GTCATGGCTTTGAACCTAAGATTGGTCAAAATGCGCCACAAATTGTTTCGATTGTCTCATTGGTGGCGGCAAACCTTGGC
GTTTCATTGGTGCCAGAATCGACCAAGCAACTCGGAATTACAGGAGTGAAATATCTAAATATTAATGATCCTACTCCGAT
GGTTGGTCTTGCATTGGCATATAGAAAAAATACGCATTCACAAATTGTGATTAATTTTTCTAGCTTAATTCATTCATTAT
TGATTCAGCAATATCAAGGGTATACAGCCTGA

Upstream 100 bases:

>100_bases
TTTGTTGGCAGTGCTTGCCTGACTGCCAACACGGTTAGATTAGCAATTAGGTGTTTATTTGTCTAATATATAAAAATAGG
TAACGTTATATGTTTTATAT

Downstream 100 bases:

>100_bases
CGGCATCTAAGCGTTGTACTAAAAAAGGCCTGATGATCTGATCAGGCCTTAGAGTTTAAATTATTTGGCTTCAAATAAAG
TTGATAATGGAATGGATAAT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Als operon regulatory protein [H]

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MELRHIRYFLVVAEEKSFTRAAARLGISQPPLSMQIKNLEDEIGTELFYRSAQGTELTPAGKAFLVSVQPIQERIAEAVQ
LAQQVANGESGQLRLGFTGTSMLNLILPKSIRLFQHQYPKIRLKLEEANSLLLMDRLLDDRLDIALVRPPDHVPEGLHLQ
LLMHEPLLAVLPIGHPFAQHHSIQLNALKDDSFIVSPRAISAGLFDAIANACRSHGFEPKIGQNAPQIVSIVSLVAANLG
VSLVPESTKQLGITGVKYLNINDPTPMVGLALAYRKNTHSQIVINFSSLIHSLLIQQYQGYTA

Sequences:

>Translated_303_residues
MELRHIRYFLVVAEEKSFTRAAARLGISQPPLSMQIKNLEDEIGTELFYRSAQGTELTPAGKAFLVSVQPIQERIAEAVQ
LAQQVANGESGQLRLGFTGTSMLNLILPKSIRLFQHQYPKIRLKLEEANSLLLMDRLLDDRLDIALVRPPDHVPEGLHLQ
LLMHEPLLAVLPIGHPFAQHHSIQLNALKDDSFIVSPRAISAGLFDAIANACRSHGFEPKIGQNAPQIVSIVSLVAANLG
VSLVPESTKQLGITGVKYLNINDPTPMVGLALAYRKNTHSQIVINFSSLIHSLLIQQYQGYTA
>Mature_303_residues
MELRHIRYFLVVAEEKSFTRAAARLGISQPPLSMQIKNLEDEIGTELFYRSAQGTELTPAGKAFLVSVQPIQERIAEAVQ
LAQQVANGESGQLRLGFTGTSMLNLILPKSIRLFQHQYPKIRLKLEEANSLLLMDRLLDDRLDIALVRPPDHVPEGLHLQ
LLMHEPLLAVLPIGHPFAQHHSIQLNALKDDSFIVSPRAISAGLFDAIANACRSHGFEPKIGQNAPQIVSIVSLVAANLG
VSLVPESTKQLGITGVKYLNINDPTPMVGLALAYRKNTHSQIVINFSSLIHSLLIQQYQGYTA

Specific function: Regulates the expression of the alsSD operon for acetoin biosynthesis [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=281, Percent_Identity=36.2989323843416, Blast_Score=183, Evalue=1e-47,
Organism=Escherichia coli, GI1788887, Length=294, Percent_Identity=31.9727891156463, Blast_Score=141, Evalue=6e-35,
Organism=Escherichia coli, GI2367136, Length=265, Percent_Identity=30.188679245283, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI157672245, Length=275, Percent_Identity=31.6363636363636, Blast_Score=116, Evalue=1e-27,
Organism=Escherichia coli, GI1790399, Length=291, Percent_Identity=27.8350515463918, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI1788297, Length=249, Percent_Identity=27.710843373494, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI87082132, Length=255, Percent_Identity=27.0588235294118, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1787601, Length=285, Percent_Identity=25.9649122807018, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI1788748, Length=243, Percent_Identity=25.1028806584362, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI87081904, Length=204, Percent_Identity=30.3921568627451, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI145693105, Length=187, Percent_Identity=25.668449197861, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787530, Length=246, Percent_Identity=24.7967479674797, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1787806, Length=254, Percent_Identity=28.3464566929134, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1790208, Length=247, Percent_Identity=24.6963562753036, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789173, Length=168, Percent_Identity=30.952380952381, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1787589, Length=124, Percent_Identity=33.0645161290323, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1788481, Length=166, Percent_Identity=28.3132530120482, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1790262, Length=264, Percent_Identity=22.7272727272727, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1789506, Length=281, Percent_Identity=23.4875444839858, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1786401, Length=209, Percent_Identity=26.3157894736842, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1789204, Length=245, Percent_Identity=26.530612244898, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI145693193, Length=120, Percent_Identity=32.5, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33409; Mature: 33409

Theoretical pI: Translated: 7.90; Mature: 7.90

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELRHIRYFLVVAEEKSFTRAAARLGISQPPLSMQIKNLEDEIGTELFYRSAQGTELTPA
CCCCEEEEEEEEECCCHHHHHHHHCCCCCCCCCEECCCHHHHHCHHHHEECCCCCCCCCC
GKAFLVSVQPIQERIAEAVQLAQQVANGESGQLRLGFTGTSMLNLILPKSIRLFQHQYPK
CCEEEEEEHHHHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHCCHHHHHHHCCCCE
IRLKLEEANSLLLMDRLLDDRLDIALVRPPDHVPEGLHLQLLMHEPLLAVLPIGHPFAQH
EEEEEECCCCCHHHHHHHCCCCCEEEECCCCCCCCCCEEEEEECCCCEEEEECCCCCCCC
HSIQLNALKDDSFIVSPRAISAGLFDAIANACRSHGFEPKIGQNAPQIVSIVSLVAANLG
CCEEEEEECCCCEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCC
VSLVPESTKQLGITGVKYLNINDPTPMVGLALAYRKNTHSQIVINFSSLIHSLLIQQYQG
CEECCCCHHHCCCCEEEEEECCCCCCHHHHHEEEECCCCCEEEEEHHHHHHHHHHHHHCC
YTA
CCC
>Mature Secondary Structure
MELRHIRYFLVVAEEKSFTRAAARLGISQPPLSMQIKNLEDEIGTELFYRSAQGTELTPA
CCCCEEEEEEEEECCCHHHHHHHHCCCCCCCCCEECCCHHHHHCHHHHEECCCCCCCCCC
GKAFLVSVQPIQERIAEAVQLAQQVANGESGQLRLGFTGTSMLNLILPKSIRLFQHQYPK
CCEEEEEEHHHHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHCCHHHHHHHCCCCE
IRLKLEEANSLLLMDRLLDDRLDIALVRPPDHVPEGLHLQLLMHEPLLAVLPIGHPFAQH
EEEEEECCCCCHHHHHHHCCCCCEEEECCCCCCCCCCEEEEEECCCCEEEEECCCCCCCC
HSIQLNALKDDSFIVSPRAISAGLFDAIANACRSHGFEPKIGQNAPQIVSIVSLVAANLG
CCEEEEEECCCCEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCC
VSLVPESTKQLGITGVKYLNINDPTPMVGLALAYRKNTHSQIVINFSSLIHSLLIQQYQG
CEECCCCHHHCCCCEEEEEECCCCCCHHHHHEEEECCCCCEEEEEHHHHHHHHHHHHHCC
YTA
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7685336; 9353933; 9384377 [H]