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The map label for this gene is prfC

Identifier: 50086104

GI number: 50086104

Start: 3020131

End: 3021720

Strand: Reverse

Name: prfC

Synonym: ACIAD3095

Alternate gene names: 50086104

Gene position: 3021720-3020131 (Counterclockwise)

Preceding gene: 50086105

Following gene: 50086103

Centisome position: 83.97

GC content: 43.84

Gene sequence:

>1590_bases
ATGTTTAAAGACCAATTAGCGAAAGAAGTCAATTCGCGCCGTACTTTTGCCATTATTTCCCACCCCGATGCGGGTAAAAC
CACCATGACAGAAAAATTGCTGTTATGGGGCAAGGCTATTCAGGTTGCAGGTATGGTGAAAAGCCGTAAATCTGATCGCT
CTGCAACTTCCGACTGGATGGAAATGGAAAAAGAACGTGGGATTTCGATCACCACTTCGGTGATGCAGTTTCCCTATAAA
CAGCATACGATCAACTTGCTAGATACCCCAGGACATGAGGACTTTTCGGAAGATACCTATCGTACTTTGACTGCGGTAGA
CTCTGCACTGATGGTCATTGATGGGGCTAAAGGGGTAGAGGAACGTACTATTAAATTGATGGAAGTGTGTCGTATGCGCG
ATACGCCAATCATCTCTTTTGTCAATAAAATGGACCGTGAGATTCGTGAACCCTTAGAACTTTTGGACGAAATTGAAAAC
GTTCTGAATATTCGCTGTGTGCCAATTACATGGCCTTTAGGGATGGGGCGTGATTTTGCAGGCGTCTATAACCTGCTTGA
AAATAAACTTTATCTTTACAAGGCAGGATTTGGCTCAACCATTACCGATATCGAAGTACGTGATGGCTATGACCATGCCG
ATGTTCGTGAAAAAGTAGGTGAGTTGGCTTGGGCTTCTTTTGAGGAATCGCTTGAGCTGGTGCAAATGGCCAATGAGCCA
CTTGATCGGGAATTATTTTTACAAGGTAAGCAAACGCCTGTTTTATTTGGAACCGCACTGGGTAATTTTGCGGTGGATCA
TGTGCTGGATGCTTTTATCAATTGGGCACCAGAACCCAAAGCACATCCAGCACAAGAGCGGAATGTGGACGCAACCGAAG
AAGGTTTTAGCGGTTTTGTATTTAAAATTCAGGCCAATATGGATCCGAAACACCGAGATCGTATTGCCTTTATGCGGATT
TGTTCAGGCAAATATGAAAAAGGCCTGAAAATGAACCATGTGCGTATTGGTAAAGATGTTCGTATTTCTGATGCGCTGAC
TTTTTTGGCAGGTGAGCGTGAACATCTTGAAGAAGCATGGCCAGGCGACATTATTGGTTTGCACAACCATGGCACCATTC
AGATTGGTGATACATTCACAAGCGGTGAAAAATTACATTTCACAGGAATTCCGCACTTTGCCCCTGAAATGTTCCGTCGT
GTTCGCTTACGTGATCCATTAAAGTCTAAACAGTTGCAAAAAGGCTTGAAAGAACTGTCAGAAGAAGGTGCAACACAGGT
GTTTATGCCACAAATCAGTAATGACCTGATTGTGGGGGCAGTGGGGGTGCTACAGTTTGATGTGGTGGCTTACCGCTTAA
AAGAAGAATACAAAGTCGATTGTATTTACGAGCCGATCAGTGTGAATACCGTGCGTTGGGTTCATTGCGATGATGAAAAG
ATTTTTAATGAATTTAAGAAAAAAGCCCACGATCAACTTTCGGTCGATGGTGGTGGACACTTAACTTATTTGGCACCAAG
TCGTGTCAATCTACAACTGATGCAGGAGCGCTGGCCTGATATTCAGTTCCGAAATACACGAGAACACTAA

Upstream 100 bases:

>100_bases
CGCCAAATCTGCTACAATTGCGGCAATTTTGAATTCCCACTTTTCAGCGCTGACATCATACGCTGTGATGACAGGCCGTA
GATAGATTGAAGAACTTTTC

Downstream 100 bases:

>100_bases
ATAATGTAAAAAACAGCGTCTAAACAGACGCTGTTTTTTATTCTAGAGTAAAGCTTTTAATTGGCATGGATAATAATAAT
GCGTGGGCTAAGATGGTTAT

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3

Number of amino acids: Translated: 529; Mature: 529

Protein sequence:

>529_residues
MFKDQLAKEVNSRRTFAIISHPDAGKTTMTEKLLLWGKAIQVAGMVKSRKSDRSATSDWMEMEKERGISITTSVMQFPYK
QHTINLLDTPGHEDFSEDTYRTLTAVDSALMVIDGAKGVEERTIKLMEVCRMRDTPIISFVNKMDREIREPLELLDEIEN
VLNIRCVPITWPLGMGRDFAGVYNLLENKLYLYKAGFGSTITDIEVRDGYDHADVREKVGELAWASFEESLELVQMANEP
LDRELFLQGKQTPVLFGTALGNFAVDHVLDAFINWAPEPKAHPAQERNVDATEEGFSGFVFKIQANMDPKHRDRIAFMRI
CSGKYEKGLKMNHVRIGKDVRISDALTFLAGEREHLEEAWPGDIIGLHNHGTIQIGDTFTSGEKLHFTGIPHFAPEMFRR
VRLRDPLKSKQLQKGLKELSEEGATQVFMPQISNDLIVGAVGVLQFDVVAYRLKEEYKVDCIYEPISVNTVRWVHCDDEK
IFNEFKKKAHDQLSVDGGGHLTYLAPSRVNLQLMQERWPDIQFRNTREH

Sequences:

>Translated_529_residues
MFKDQLAKEVNSRRTFAIISHPDAGKTTMTEKLLLWGKAIQVAGMVKSRKSDRSATSDWMEMEKERGISITTSVMQFPYK
QHTINLLDTPGHEDFSEDTYRTLTAVDSALMVIDGAKGVEERTIKLMEVCRMRDTPIISFVNKMDREIREPLELLDEIEN
VLNIRCVPITWPLGMGRDFAGVYNLLENKLYLYKAGFGSTITDIEVRDGYDHADVREKVGELAWASFEESLELVQMANEP
LDRELFLQGKQTPVLFGTALGNFAVDHVLDAFINWAPEPKAHPAQERNVDATEEGFSGFVFKIQANMDPKHRDRIAFMRI
CSGKYEKGLKMNHVRIGKDVRISDALTFLAGEREHLEEAWPGDIIGLHNHGTIQIGDTFTSGEKLHFTGIPHFAPEMFRR
VRLRDPLKSKQLQKGLKELSEEGATQVFMPQISNDLIVGAVGVLQFDVVAYRLKEEYKVDCIYEPISVNTVRWVHCDDEK
IFNEFKKKAHDQLSVDGGGHLTYLAPSRVNLQLMQERWPDIQFRNTREH
>Mature_529_residues
MFKDQLAKEVNSRRTFAIISHPDAGKTTMTEKLLLWGKAIQVAGMVKSRKSDRSATSDWMEMEKERGISITTSVMQFPYK
QHTINLLDTPGHEDFSEDTYRTLTAVDSALMVIDGAKGVEERTIKLMEVCRMRDTPIISFVNKMDREIREPLELLDEIEN
VLNIRCVPITWPLGMGRDFAGVYNLLENKLYLYKAGFGSTITDIEVRDGYDHADVREKVGELAWASFEESLELVQMANEP
LDRELFLQGKQTPVLFGTALGNFAVDHVLDAFINWAPEPKAHPAQERNVDATEEGFSGFVFKIQANMDPKHRDRIAFMRI
CSGKYEKGLKMNHVRIGKDVRISDALTFLAGEREHLEEAWPGDIIGLHNHGTIQIGDTFTSGEKLHFTGIPHFAPEMFRR
VRLRDPLKSKQLQKGLKELSEEGATQVFMPQISNDLIVGAVGVLQFDVVAYRLKEEYKVDCIYEPISVNTVRWVHCDDEK
IFNEFKKKAHDQLSVDGGGHLTYLAPSRVNLQLMQERWPDIQFRNTREH

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily

Homologues:

Organism=Homo sapiens, GI18390331, Length=483, Percent_Identity=26.7080745341615, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI19923640, Length=509, Percent_Identity=25.5402750491159, Blast_Score=141, Evalue=2e-33,
Organism=Homo sapiens, GI25306283, Length=394, Percent_Identity=27.4111675126904, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI25306287, Length=478, Percent_Identity=23.6401673640167, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI157426893, Length=172, Percent_Identity=30.8139534883721, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI94966754, Length=166, Percent_Identity=29.5180722891566, Blast_Score=79, Evalue=8e-15,
Organism=Homo sapiens, GI4503483, Length=150, Percent_Identity=32, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI310132016, Length=119, Percent_Identity=31.9327731092437, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI310110807, Length=119, Percent_Identity=31.9327731092437, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI310123363, Length=119, Percent_Identity=31.9327731092437, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1790835, Length=522, Percent_Identity=65.9003831417624, Blast_Score=732, Evalue=0.0,
Organism=Escherichia coli, GI1789738, Length=494, Percent_Identity=28.5425101214575, Blast_Score=161, Evalue=9e-41,
Organism=Escherichia coli, GI1788922, Length=156, Percent_Identity=33.3333333333333, Blast_Score=99, Evalue=8e-22,
Organism=Escherichia coli, GI48994988, Length=145, Percent_Identity=34.4827586206897, Blast_Score=85, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17533571, Length=498, Percent_Identity=27.9116465863454, Blast_Score=173, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17556745, Length=179, Percent_Identity=36.8715083798883, Blast_Score=118, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI17557151, Length=132, Percent_Identity=31.0606060606061, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI71988819, Length=133, Percent_Identity=30.0751879699248, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71988811, Length=133, Percent_Identity=30.0751879699248, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323098, Length=484, Percent_Identity=27.0661157024793, Blast_Score=180, Evalue=5e-46,
Organism=Saccharomyces cerevisiae, GI6322359, Length=134, Percent_Identity=38.8059701492537, Blast_Score=100, Evalue=8e-22,
Organism=Saccharomyces cerevisiae, GI6324707, Length=162, Percent_Identity=32.0987654320988, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6320593, Length=162, Percent_Identity=32.0987654320988, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323320, Length=153, Percent_Identity=26.1437908496732, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24582462, Length=488, Percent_Identity=27.0491803278689, Blast_Score=168, Evalue=9e-42,
Organism=Drosophila melanogaster, GI221458488, Length=503, Percent_Identity=25.6461232604374, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI78706572, Length=170, Percent_Identity=27.6470588235294, Blast_Score=88, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24585709, Length=158, Percent_Identity=31.0126582278481, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24585711, Length=158, Percent_Identity=31.0126582278481, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24585713, Length=158, Percent_Identity=31.0126582278481, Blast_Score=71, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RF3_ACIAD (Q6F823)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_047614.1
- ProteinModelPortal:   Q6F823
- SMR:   Q6F823
- STRING:   Q6F823
- GeneID:   2880041
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD3095
- NMPDR:   fig|62977.3.peg.2807
- eggNOG:   COG4108
- HOGENOM:   HBG285678
- OMA:   HERTVEP
- PhylomeDB:   Q6F823
- ProtClustDB:   PRK00741
- BioCyc:   ASP62977:ACIAD3095-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00072
- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000
- PRINTS:   PR00315
- TIGRFAMs:   TIGR00503
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; SSF54980 EFG_III_V; SSF50447 Translat_factor

EC number: NA

Molecular weight: Translated: 60376; Mature: 60376

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKDQLAKEVNSRRTFAIISHPDAGKTTMTEKLLLWGKAIQVAGMVKSRKSDRSATSDWM
CCHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
EMEKERGISITTSVMQFPYKQHTINLLDTPGHEDFSEDTYRTLTAVDSALMVIDGAKGVE
HHHHHCCCEEEHHHHHCCCHHCEEEEEECCCCCCCCHHHHHHHHHHCCEEEEEECCCCHH
ERTIKLMEVCRMRDTPIISFVNKMDREIREPLELLDEIENVLNIRCVPITWPLGMGRDFA
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEECCCCCCHHH
GVYNLLENKLYLYKAGFGSTITDIEVRDGYDHADVREKVGELAWASFEESLELVQMANEP
HHHHHHHCCEEEEECCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LDRELFLQGKQTPVLFGTALGNFAVDHVLDAFINWAPEPKAHPAQERNVDATEEGFSGFV
CCHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEE
FKIQANMDPKHRDRIAFMRICSGKYEKGLKMNHVRIGKDVRISDALTFLAGEREHLEEAW
EEEECCCCCCHHHHHHHHHHHCCHHHCCCEECEEEECCCCCHHHHHHHHCCCHHHHHHCC
PGDIIGLHNHGTIQIGDTFTSGEKLHFTGIPHFAPEMFRRVRLRDPLKSKQLQKGLKELS
CCCEEEECCCCEEEECCEECCCCEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
EEGATQVFMPQISNDLIVGAVGVLQFDVVAYRLKEEYKVDCIYEPISVNTVRWVHCDDEK
HCCCCEEEECCCCCCEEEHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEEECCHHH
IFNEFKKKAHDQLSVDGGGHLTYLAPSRVNLQLMQERWPDIQFRNTREH
HHHHHHHHHCCEEEECCCCEEEEECCCCCHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MFKDQLAKEVNSRRTFAIISHPDAGKTTMTEKLLLWGKAIQVAGMVKSRKSDRSATSDWM
CCHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
EMEKERGISITTSVMQFPYKQHTINLLDTPGHEDFSEDTYRTLTAVDSALMVIDGAKGVE
HHHHHCCCEEEHHHHHCCCHHCEEEEEECCCCCCCCHHHHHHHHHHCCEEEEEECCCCHH
ERTIKLMEVCRMRDTPIISFVNKMDREIREPLELLDEIENVLNIRCVPITWPLGMGRDFA
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEECCCCCCHHH
GVYNLLENKLYLYKAGFGSTITDIEVRDGYDHADVREKVGELAWASFEESLELVQMANEP
HHHHHHHCCEEEEECCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LDRELFLQGKQTPVLFGTALGNFAVDHVLDAFINWAPEPKAHPAQERNVDATEEGFSGFV
CCHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEE
FKIQANMDPKHRDRIAFMRICSGKYEKGLKMNHVRIGKDVRISDALTFLAGEREHLEEAW
EEEECCCCCCHHHHHHHHHHHCCHHHCCCEECEEEECCCCCHHHHHHHHCCCHHHHHHCC
PGDIIGLHNHGTIQIGDTFTSGEKLHFTGIPHFAPEMFRRVRLRDPLKSKQLQKGLKELS
CCCEEEECCCCEEEECCEECCCCEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
EEGATQVFMPQISNDLIVGAVGVLQFDVVAYRLKEEYKVDCIYEPISVNTVRWVHCDDEK
HCCCCEEEECCCCCCEEEHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEEECCHHH
IFNEFKKKAHDQLSVDGGGHLTYLAPSRVNLQLMQERWPDIQFRNTREH
HHHHHHHHHCCEEEECCCCEEEEECCCCCHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA