Click here to switch to the map view.

The map label for this gene is yngF [H]

Identifier: 50086084

GI number: 50086084

Start: 3001345

End: 3002133

Strand: Direct

Name: yngF [H]

Synonym: ACIAD3073

Alternate gene names: 50086084

Gene position: 3001345-3002133 (Clockwise)

Preceding gene: 50086083

Following gene: 50086085

Centisome position: 83.4

GC content: 44.74

Gene sequence:

>789_bases
ATGGAATCGATCAAACTCACGATATCTCAAGGCATCGCACAGGTTACTTTAAACCGTCCAGAAAAACGTAACGCGATGAG
TTTCAATCTGCTTCGTGAGCTTATTCAGACAGCTAAAACCATCAAAAAAGACCGCTCTGTTCGTTGTGTCATCCTAAGTG
GTGAAGCGCATGTCTTTAGTGCAGGTATTGATTTAAGCGACCTGAATAATCCTAAAAATACAGTATATGCGGCATGGGAA
TTAATTAAACCAGGACAAAGCCTGTTCCAAAAGGCATTTTTGATCTGGCAAAGCCTGCCTGTGCCTGTAATTGCTGTCAT
TGAAGGTTATTGTTTTGGCGCAGGCATGCAATTGGCACTCGCTGCCGATATTCGGATTGCTCATCCAGAAACACAAATGT
CAATTATGGAAAGCCGCTGGGGTCTTGTTCCAGATATGGGACTTACTCGATCAATCAAAGGACTAATTGGATCAGATCTT
GCCAAAGAGCTCACACTGACTGCACGAATTTTCGATGCAGAGTACGCACATGACATTGGTCTGGTCACTCATCTTTCAGC
ACAGCCATGGCAGCATGCACAACAACTAGCCAGCGAGTTGCAACAGCGTTCCCCAGATGCACTGGCCGCAGCGAAATTTG
TCTTAAATGCCATGGAACATCAACCTCAGCGTGCCTTACGCCTGGAGAAGCTGTGGCAGTTAAAGTTATTATTGGGTAAA
AATAGTCGACTCGCGCGACAAAAGGACAAACATCCCGAAGTAGAGTTTTTGCCTCGTCAATATCAATAA

Upstream 100 bases:

>100_bases
TTTTATCCGAGCAGCAATAAATCTATGACTGAGCTGTTCTTTTTAAAGAGACAGCTCATGTTATGCTCAGGATTATATAT
TTTTAGATGAGTCCTCAGAC

Downstream 100 bases:

>100_bases
TCAAGGATTACATTATGCGTTTTGATCGTCACACGCCTATTGCTTTTATCGGGATGGGTTTAATGGGTAGCCGCATGGCG
ACACGACTGGTTCAGGCAGG

Product: enoyl-CoA hydratase

Products: trans(or 3)-enoyl-CoA; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MESIKLTISQGIAQVTLNRPEKRNAMSFNLLRELIQTAKTIKKDRSVRCVILSGEAHVFSAGIDLSDLNNPKNTVYAAWE
LIKPGQSLFQKAFLIWQSLPVPVIAVIEGYCFGAGMQLALAADIRIAHPETQMSIMESRWGLVPDMGLTRSIKGLIGSDL
AKELTLTARIFDAEYAHDIGLVTHLSAQPWQHAQQLASELQQRSPDALAAAKFVLNAMEHQPQRALRLEKLWQLKLLLGK
NSRLARQKDKHPEVEFLPRQYQ

Sequences:

>Translated_262_residues
MESIKLTISQGIAQVTLNRPEKRNAMSFNLLRELIQTAKTIKKDRSVRCVILSGEAHVFSAGIDLSDLNNPKNTVYAAWE
LIKPGQSLFQKAFLIWQSLPVPVIAVIEGYCFGAGMQLALAADIRIAHPETQMSIMESRWGLVPDMGLTRSIKGLIGSDL
AKELTLTARIFDAEYAHDIGLVTHLSAQPWQHAQQLASELQQRSPDALAAAKFVLNAMEHQPQRALRLEKLWQLKLLLGK
NSRLARQKDKHPEVEFLPRQYQ
>Mature_262_residues
MESIKLTISQGIAQVTLNRPEKRNAMSFNLLRELIQTAKTIKKDRSVRCVILSGEAHVFSAGIDLSDLNNPKNTVYAAWE
LIKPGQSLFQKAFLIWQSLPVPVIAVIEGYCFGAGMQLALAADIRIAHPETQMSIMESRWGLVPDMGLTRSIKGLIGSDL
AKELTLTARIFDAEYAHDIGLVTHLSAQPWQHAQQLASELQQRSPDALAAAKFVLNAMEHQPQRALRLEKLWQLKLLLGK
NSRLARQKDKHPEVEFLPRQYQ

Specific function: Could Possibly Oxidizes Fatty Acids Using Specific Components (By Similarity). [C]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI70995211, Length=248, Percent_Identity=33.0645161290323, Blast_Score=115, Evalue=5e-26,
Organism=Homo sapiens, GI4502327, Length=215, Percent_Identity=32.5581395348837, Blast_Score=98, Evalue=7e-21,
Organism=Homo sapiens, GI194097323, Length=226, Percent_Identity=30.9734513274336, Blast_Score=93, Evalue=2e-19,
Organism=Homo sapiens, GI157694516, Length=230, Percent_Identity=24.3478260869565, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI213417737, Length=230, Percent_Identity=24.3478260869565, Blast_Score=78, Evalue=9e-15,
Organism=Homo sapiens, GI68989263, Length=175, Percent_Identity=27.4285714285714, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI31542718, Length=181, Percent_Identity=28.1767955801105, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1787659, Length=220, Percent_Identity=30.4545454545455, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI221142681, Length=227, Percent_Identity=30.3964757709251, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1788682, Length=175, Percent_Identity=33.1428571428571, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1787660, Length=243, Percent_Identity=30.8641975308642, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1788597, Length=223, Percent_Identity=26.9058295964126, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1790281, Length=179, Percent_Identity=24.5810055865922, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI87082183, Length=212, Percent_Identity=25.4716981132075, Blast_Score=63, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17536985, Length=243, Percent_Identity=30.4526748971193, Blast_Score=117, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI17540714, Length=209, Percent_Identity=31.1004784688995, Blast_Score=114, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17534483, Length=246, Percent_Identity=28.0487804878049, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17554946, Length=233, Percent_Identity=27.8969957081545, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI25145438, Length=228, Percent_Identity=28.0701754385965, Blast_Score=80, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI25141363, Length=238, Percent_Identity=25.2100840336134, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI25144276, Length=236, Percent_Identity=29.2372881355932, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17508953, Length=236, Percent_Identity=29.2372881355932, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17508951, Length=236, Percent_Identity=29.2372881355932, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17560910, Length=174, Percent_Identity=25.8620689655172, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17558304, Length=245, Percent_Identity=26.9387755102041, Blast_Score=68, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24653139, Length=214, Percent_Identity=34.5794392523364, Blast_Score=125, Evalue=2e-29,
Organism=Drosophila melanogaster, GI19920382, Length=226, Percent_Identity=30.0884955752212, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI20129971, Length=239, Percent_Identity=26.7782426778243, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24653477, Length=239, Percent_Identity=26.7782426778243, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI21357171, Length=219, Percent_Identity=29.2237442922374, Blast_Score=78, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24583165, Length=183, Percent_Identity=25.1366120218579, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI45550169, Length=198, Percent_Identity=24.2424242424242, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI19921018, Length=198, Percent_Identity=21.7171717171717, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI19922422, Length=235, Percent_Identity=25.1063829787234, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.17 [C]

Molecular weight: Translated: 29397; Mature: 29397

Theoretical pI: Translated: 9.86; Mature: 9.86

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESIKLTISQGIAQVTLNRPEKRNAMSFNLLRELIQTAKTIKKDRSVRCVILSGEAHVFS
CCCEEEEHHCCHHEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCEEEE
AGIDLSDLNNPKNTVYAAWELIKPGQSLFQKAFLIWQSLPVPVIAVIEGYCFGAGMQLAL
CCCCHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEE
AADIRIAHPETQMSIMESRWGLVPDMGLTRSIKGLIGSDLAKELTLTARIFDAEYAHDIG
EECEEEECCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVTHLSAQPWQHAQQLASELQQRSPDALAAAKFVLNAMEHQPQRALRLEKLWQLKLLLGK
HHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCC
NSRLARQKDKHPEVEFLPRQYQ
CCHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MESIKLTISQGIAQVTLNRPEKRNAMSFNLLRELIQTAKTIKKDRSVRCVILSGEAHVFS
CCCEEEEHHCCHHEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCEEEE
AGIDLSDLNNPKNTVYAAWELIKPGQSLFQKAFLIWQSLPVPVIAVIEGYCFGAGMQLAL
CCCCHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEE
AADIRIAHPETQMSIMESRWGLVPDMGLTRSIKGLIGSDLAKELTLTARIFDAEYAHDIG
EECEEEECCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVTHLSAQPWQHAQQLASELQQRSPDALAAAKFVLNAMEHQPQRALRLEKLWQLKLLLGK
HHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCC
NSRLARQKDKHPEVEFLPRQYQ
CCHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.05 {crotonyl-CoA}} 0.008 {2-decenoyl-CoA}} [C]

Substrates: (3S)-hydroxyacyl-CoA [C]

Specific reaction: (3S)-hydroxyacyl-CoA = trans(or 3)-enoyl-CoA + H2O [C]

General reaction: addition (of H2O to carbon carbon double bondelimination (of H2O C-O bond cleavagehydration [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387222; 9384377 [H]