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The map label for this gene is alkK [H]

Identifier: 50086068

GI number: 50086068

Start: 2983528

End: 2985171

Strand: Direct

Name: alkK [H]

Synonym: ACIAD3056

Alternate gene names: 50086068

Gene position: 2983528-2985171 (Clockwise)

Preceding gene: 50086065

Following gene: 50086078

Centisome position: 82.91

GC content: 44.65

Gene sequence:

>1644_bases
ATGGTGAGTGCTTATGATGAACTTCCTCGCACACCTGCCAATTTTGTTGCATTATCACCATTACGTTATTTAGAACGCGC
AGCTTATATTTACCCTCAACAAGCCGCAATTATTCACGGCACACGTGTCATCACATGGCAGGAAACCTATACGCGCTGTC
GTCAGTTTGCGTCACAACTCAAACAACTTGGCATTCAAAAGAACGATACTGTTTCAGTGCTTTTGCCAAATGTGCCTGCC
ATGATTGAAGCTCATTTTGCAGTGCCAATGAGTGGTGCAGTACTCAATACCATCAATACCCGACTCGATGCCAAAACTGT
AGCCTTTATGCTGGAGCATGCAGAGACCAAAGTATTACTAGTCGATCAGGAATTTTCTGAATTAGCCAAAGCAGCGTTAT
CTTTGATCAATCAGGATATCTTTGTGATTGATGTCGATGATGCCGAGTATGAAAAATGTTTTACCCCACCCATTGGTGCA
ATTGAATATGAGGACTGGATTTCTGAGGGTGATGAATATTTTGAATGGATTCTTCCCAACGACGAGTGGGATGCGATTAG
CTTAAACTATACTTCGGGGACCACAGGAAACCCCAAAGGTGTAGTGTATCACCACCGCGGTGCATATATTAATGCAGCCA
GTAATATTATTGCCTGTGGTATGACCCCACGTGCCAAGTATCTCTGGACGCTACCGCTATTTCACTGTAATGGCTGGTGT
TTTGCTTGGACGGTTGCTGCAAATGGCGGCACCAATATTTGTTTGCGCAAAGTCGATCCTGCCCTGGTTTTTAAACTCAT
TGAACAACATAAAATCGATTATTTCTGTGGAGCACCGATTGTTTTATCAATGCTGATTAATGCACCTAAACAACATCAAA
TCAAATTTAATCATCGTGTTGAAGTGATGGTAGCGGGTGCCGCCCCACCTGCTGCCATTATTGAAGGTATGCGTCATCTA
GGGGTCAATGTGACCCATGTATACGGCTTAACCGAAACCTATGGGCCTTCTGGCCTGTGTGCGGCACAGGCAGGCTGGAG
TGATCTTTCTATTCAGGAACAAGCACAACTGCATGCACGTCAAGGGGTTCCATATCCCTTGCAGGACAGTATGCGGGTAC
TCGATCCCAAGACCATGCAACCTGTGCCTGCAGATGGACAAACATTGGGGGAAATTATGTTTCGTGGCAATATCGTGATG
AAGGGTTATCTCAAAAATCCGAAGGCGACAGAAGAAGCGTTTGCTGATGGGTGGTTCCACAGTGGCGATCTGGCAGTTTG
CCAACCTGATGGCTATGCAAAAATTACAGATCGTGCCAAAGATGTCATTATTTCAGGTGGTGAAAATATTTCTTCTCTTG
AGGTTGAGGAGGTGCTATACCGTCATCCTGCCGTGCTGACTGCGGCAGTAGTTGCTAAACCTGATCCGCAATGGCAGGAA
GTCCCATGTGCTTTCATTGAGTTAAAAGCAGGCGTAAATATCACCACGGAAGAGCTTATTGAACATTGCAAAAAGGATTT
GGCTCGCTTTAAGGTTCCTAAAGATATTGTAATCACCGAAATTCCCAAAACCTCAACAGGTAAATTACAAAAATTTATTT
TACGAGATTGGGCAAAAGAGCGCGCTACAGGCCAATTCACTTAG

Upstream 100 bases:

>100_bases
TGAAAAAAAGATCTTATTCTAAGACTAAAGCATAAATGATTTGATGAATTCAAACCCATATCTTTTTAAACAGATTAGAA
CAATTACATGGAGTATCAAT

Downstream 100 bases:

>100_bases
ATCAATTGACCAATAAAAAGCCAGTCCATCGTCAGGAACTGGCTTTTTAATGTGCAACATAGATTCAGTGCATATCACAT
TTGTTTAGTTATCTGACAGA

Product: acyl-CoA synthetase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Medium-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 547; Mature: 547

Protein sequence:

>547_residues
MVSAYDELPRTPANFVALSPLRYLERAAYIYPQQAAIIHGTRVITWQETYTRCRQFASQLKQLGIQKNDTVSVLLPNVPA
MIEAHFAVPMSGAVLNTINTRLDAKTVAFMLEHAETKVLLVDQEFSELAKAALSLINQDIFVIDVDDAEYEKCFTPPIGA
IEYEDWISEGDEYFEWILPNDEWDAISLNYTSGTTGNPKGVVYHHRGAYINAASNIIACGMTPRAKYLWTLPLFHCNGWC
FAWTVAANGGTNICLRKVDPALVFKLIEQHKIDYFCGAPIVLSMLINAPKQHQIKFNHRVEVMVAGAAPPAAIIEGMRHL
GVNVTHVYGLTETYGPSGLCAAQAGWSDLSIQEQAQLHARQGVPYPLQDSMRVLDPKTMQPVPADGQTLGEIMFRGNIVM
KGYLKNPKATEEAFADGWFHSGDLAVCQPDGYAKITDRAKDVIISGGENISSLEVEEVLYRHPAVLTAAVVAKPDPQWQE
VPCAFIELKAGVNITTEELIEHCKKDLARFKVPKDIVITEIPKTSTGKLQKFILRDWAKERATGQFT

Sequences:

>Translated_547_residues
MVSAYDELPRTPANFVALSPLRYLERAAYIYPQQAAIIHGTRVITWQETYTRCRQFASQLKQLGIQKNDTVSVLLPNVPA
MIEAHFAVPMSGAVLNTINTRLDAKTVAFMLEHAETKVLLVDQEFSELAKAALSLINQDIFVIDVDDAEYEKCFTPPIGA
IEYEDWISEGDEYFEWILPNDEWDAISLNYTSGTTGNPKGVVYHHRGAYINAASNIIACGMTPRAKYLWTLPLFHCNGWC
FAWTVAANGGTNICLRKVDPALVFKLIEQHKIDYFCGAPIVLSMLINAPKQHQIKFNHRVEVMVAGAAPPAAIIEGMRHL
GVNVTHVYGLTETYGPSGLCAAQAGWSDLSIQEQAQLHARQGVPYPLQDSMRVLDPKTMQPVPADGQTLGEIMFRGNIVM
KGYLKNPKATEEAFADGWFHSGDLAVCQPDGYAKITDRAKDVIISGGENISSLEVEEVLYRHPAVLTAAVVAKPDPQWQE
VPCAFIELKAGVNITTEELIEHCKKDLARFKVPKDIVITEIPKTSTGKLQKFILRDWAKERATGQFT
>Mature_547_residues
MVSAYDELPRTPANFVALSPLRYLERAAYIYPQQAAIIHGTRVITWQETYTRCRQFASQLKQLGIQKNDTVSVLLPNVPA
MIEAHFAVPMSGAVLNTINTRLDAKTVAFMLEHAETKVLLVDQEFSELAKAALSLINQDIFVIDVDDAEYEKCFTPPIGA
IEYEDWISEGDEYFEWILPNDEWDAISLNYTSGTTGNPKGVVYHHRGAYINAASNIIACGMTPRAKYLWTLPLFHCNGWC
FAWTVAANGGTNICLRKVDPALVFKLIEQHKIDYFCGAPIVLSMLINAPKQHQIKFNHRVEVMVAGAAPPAAIIEGMRHL
GVNVTHVYGLTETYGPSGLCAAQAGWSDLSIQEQAQLHARQGVPYPLQDSMRVLDPKTMQPVPADGQTLGEIMFRGNIVM
KGYLKNPKATEEAFADGWFHSGDLAVCQPDGYAKITDRAKDVIISGGENISSLEVEEVLYRHPAVLTAAVVAKPDPQWQE
VPCAFIELKAGVNITTEELIEHCKKDLARFKVPKDIVITEIPKTSTGKLQKFILRDWAKERATGQFT

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=550, Percent_Identity=26.9090909090909, Blast_Score=167, Evalue=3e-41,
Organism=Homo sapiens, GI187761345, Length=571, Percent_Identity=25.569176882662, Blast_Score=145, Evalue=1e-34,
Organism=Homo sapiens, GI187761343, Length=571, Percent_Identity=25.569176882662, Blast_Score=145, Evalue=1e-34,
Organism=Homo sapiens, GI157311624, Length=488, Percent_Identity=25, Blast_Score=130, Evalue=4e-30,
Organism=Homo sapiens, GI157311622, Length=488, Percent_Identity=25, Blast_Score=130, Evalue=4e-30,
Organism=Homo sapiens, GI38505220, Length=541, Percent_Identity=26.0628465804067, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI58082049, Length=491, Percent_Identity=24.847250509165, Blast_Score=124, Evalue=3e-28,
Organism=Homo sapiens, GI122937307, Length=523, Percent_Identity=25.8126195028681, Blast_Score=122, Evalue=6e-28,
Organism=Homo sapiens, GI42544132, Length=512, Percent_Identity=25.5859375, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI28416953, Length=574, Percent_Identity=23.1707317073171, Blast_Score=104, Evalue=3e-22,
Organism=Homo sapiens, GI115511026, Length=520, Percent_Identity=24.2307692307692, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI13375727, Length=554, Percent_Identity=22.7436823104693, Blast_Score=92, Evalue=2e-18,
Organism=Homo sapiens, GI42794756, Length=543, Percent_Identity=22.6519337016575, Blast_Score=88, Evalue=3e-17,
Organism=Homo sapiens, GI42794760, Length=548, Percent_Identity=23.1751824817518, Blast_Score=88, Evalue=3e-17,
Organism=Homo sapiens, GI42794758, Length=548, Percent_Identity=23.1751824817518, Blast_Score=88, Evalue=3e-17,
Organism=Homo sapiens, GI40807491, Length=391, Percent_Identity=26.5984654731458, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI57165412, Length=510, Percent_Identity=24.9019607843137, Blast_Score=76, Evalue=9e-14,
Organism=Homo sapiens, GI57165410, Length=510, Percent_Identity=24.7058823529412, Blast_Score=75, Evalue=2e-13,
Organism=Escherichia coli, GI1788107, Length=574, Percent_Identity=27.0034843205575, Blast_Score=166, Evalue=4e-42,
Organism=Escherichia coli, GI145693145, Length=359, Percent_Identity=31.7548746518106, Blast_Score=156, Evalue=4e-39,
Organism=Escherichia coli, GI1786810, Length=526, Percent_Identity=26.2357414448669, Blast_Score=149, Evalue=6e-37,
Organism=Escherichia coli, GI221142682, Length=495, Percent_Identity=25.2525252525253, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1788595, Length=360, Percent_Identity=25.8333333333333, Blast_Score=89, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71994694, Length=536, Percent_Identity=27.2388059701493, Blast_Score=173, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI71994690, Length=536, Percent_Identity=27.2388059701493, Blast_Score=173, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI71994703, Length=536, Percent_Identity=27.2388059701493, Blast_Score=173, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17560308, Length=529, Percent_Identity=27.4102079395085, Blast_Score=157, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17559526, Length=525, Percent_Identity=25.7142857142857, Blast_Score=152, Evalue=4e-37,
Organism=Caenorhabditis elegans, GI32563687, Length=519, Percent_Identity=27.3603082851638, Blast_Score=141, Evalue=8e-34,
Organism=Caenorhabditis elegans, GI17558820, Length=364, Percent_Identity=29.1208791208791, Blast_Score=136, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI17531443, Length=515, Percent_Identity=24.0776699029126, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17557194, Length=522, Percent_Identity=25.095785440613, Blast_Score=124, Evalue=9e-29,
Organism=Caenorhabditis elegans, GI17538037, Length=523, Percent_Identity=24.8565965583174, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17560140, Length=510, Percent_Identity=24.5098039215686, Blast_Score=118, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI71996755, Length=516, Percent_Identity=24.6124031007752, Blast_Score=106, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI71983001, Length=583, Percent_Identity=25.7289879931389, Blast_Score=92, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI71982997, Length=583, Percent_Identity=25.7289879931389, Blast_Score=92, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI71985884, Length=366, Percent_Identity=27.5956284153005, Blast_Score=89, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17556552, Length=518, Percent_Identity=24.7104247104247, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17510401, Length=235, Percent_Identity=28.0851063829787, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6319699, Length=502, Percent_Identity=26.0956175298805, Blast_Score=137, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6319264, Length=574, Percent_Identity=25.0871080139373, Blast_Score=100, Evalue=6e-22,
Organism=Saccharomyces cerevisiae, GI6323182, Length=291, Percent_Identity=26.1168384879725, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24581924, Length=563, Percent_Identity=26.2877442273535, Blast_Score=162, Evalue=6e-40,
Organism=Drosophila melanogaster, GI18859661, Length=526, Percent_Identity=27.7566539923954, Blast_Score=141, Evalue=1e-33,
Organism=Drosophila melanogaster, GI21355181, Length=499, Percent_Identity=26.0521042084168, Blast_Score=138, Evalue=9e-33,
Organism=Drosophila melanogaster, GI21358303, Length=503, Percent_Identity=22.8628230616302, Blast_Score=96, Evalue=7e-20,
Organism=Drosophila melanogaster, GI21356947, Length=515, Percent_Identity=23.1067961165049, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI281365686, Length=516, Percent_Identity=22.2868217054264, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24648260, Length=524, Percent_Identity=21.9465648854962, Blast_Score=92, Evalue=9e-19,
Organism=Drosophila melanogaster, GI19921316, Length=537, Percent_Identity=24.0223463687151, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI19922652, Length=500, Percent_Identity=24.6, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24653035, Length=530, Percent_Identity=21.6981132075472, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI161076582, Length=495, Percent_Identity=21.4141414141414, Blast_Score=80, Evalue=5e-15,
Organism=Drosophila melanogaster, GI20130357, Length=357, Percent_Identity=23.249299719888, Blast_Score=72, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 60749; Mature: 60749

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSAYDELPRTPANFVALSPLRYLERAAYIYPQQAAIIHGTRVITWQETYTRCRQFASQL
CCCCCHHCCCCCCCEEEECHHHHHHHHHHCCCCCEEEEECCEEEEEHHHHHHHHHHHHHH
KQLGIQKNDTVSVLLPNVPAMIEAHFAVPMSGAVLNTINTRLDAKTVAFMLEHAETKVLL
HHCCCCCCCEEEEEECCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEE
VDQEFSELAKAALSLINQDIFVIDVDDAEYEKCFTPPIGAIEYEDWISEGDEYFEWILPN
ECHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHCCCCCCCEEHHHHHCCCCCEEEEECCC
DEWDAISLNYTSGTTGNPKGVVYHHRGAYINAASNIIACGMTPRAKYLWTLPLFHCNGWC
CCCCEEEEEECCCCCCCCCEEEEECCCCEEECCCCEEEECCCCCCCEEEEEEEEECCCEE
FAWTVAANGGTNICLRKVDPALVFKLIEQHKIDYFCGAPIVLSMLINAPKQHQIKFNHRV
EEEEEEECCCCEEEEECCCHHHHHHHHHHCCCCEECCCHHHHHHHHCCCCCCEEEECCEE
EVMVAGAAPPAAIIEGMRHLGVNVTHVYGLTETYGPSGLCAAQAGWSDLSIQEQAQLHAR
EEEEECCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCCCEEECCCCCCCCHHHHHHHHHH
QGVPYPLQDSMRVLDPKTMQPVPADGQTLGEIMFRGNIVMKGYLKNPKATEEAFADGWFH
CCCCCCCCCCHHHCCCCCCCCCCCCCHHHHHHHHCCCEEEEEECCCCCHHHHHHHCCCEE
SGDLAVCQPDGYAKITDRAKDVIISGGENISSLEVEEVLYRHPAVLTAAVVAKPDPQWQE
CCCEEEECCCCCEECCCCCCEEEEECCCCCCHHHHHHHHHHCCHHHEEEEEECCCCCHHH
VPCAFIELKAGVNITTEELIEHCKKDLARFKVPKDIVITEIPKTSTGKLQKFILRDWAKE
CCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHH
RATGQFT
CCCCCCC
>Mature Secondary Structure
MVSAYDELPRTPANFVALSPLRYLERAAYIYPQQAAIIHGTRVITWQETYTRCRQFASQL
CCCCCHHCCCCCCCEEEECHHHHHHHHHHCCCCCEEEEECCEEEEEHHHHHHHHHHHHHH
KQLGIQKNDTVSVLLPNVPAMIEAHFAVPMSGAVLNTINTRLDAKTVAFMLEHAETKVLL
HHCCCCCCCEEEEEECCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEE
VDQEFSELAKAALSLINQDIFVIDVDDAEYEKCFTPPIGAIEYEDWISEGDEYFEWILPN
ECHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHCCCCCCCEEHHHHHCCCCCEEEEECCC
DEWDAISLNYTSGTTGNPKGVVYHHRGAYINAASNIIACGMTPRAKYLWTLPLFHCNGWC
CCCCEEEEEECCCCCCCCCEEEEECCCCEEECCCCEEEECCCCCCCEEEEEEEEECCCEE
FAWTVAANGGTNICLRKVDPALVFKLIEQHKIDYFCGAPIVLSMLINAPKQHQIKFNHRV
EEEEEEECCCCEEEEECCCHHHHHHHHHHCCCCEECCCHHHHHHHHCCCCCCEEEECCEE
EVMVAGAAPPAAIIEGMRHLGVNVTHVYGLTETYGPSGLCAAQAGWSDLSIQEQAQLHAR
EEEEECCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCCCEEECCCCCCCCHHHHHHHHHH
QGVPYPLQDSMRVLDPKTMQPVPADGQTLGEIMFRGNIVMKGYLKNPKATEEAFADGWFH
CCCCCCCCCCHHHCCCCCCCCCCCCCHHHHHHHHCCCEEEEEECCCCCHHHHHHHCCCEE
SGDLAVCQPDGYAKITDRAKDVIISGGENISSLEVEEVLYRHPAVLTAAVVAKPDPQWQE
CCCEEEECCCCCEECCCCCCEEEEECCCCCCHHHHHHHHHHCCHHHEEEEEECCCCCHHH
VPCAFIELKAGVNITTEELIEHCKKDLARFKVPKDIVITEIPKTSTGKLQKFILRDWAKE
CCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHH
RATGQFT
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0013 {undecanoate}} 0.0022 {pentadecanoate}} 0.053 {CoA}} 0.035 {CoA}} 0.034 {CoA}} 0.0034 {tridecanoate}} 0.002 {laurate}} 0.0019 {laurate}} 0.0

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1453953 [H]