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The map label for this gene is ksgA

Identifier: 50086027

GI number: 50086027

Start: 2936886

End: 2937710

Strand: Reverse

Name: ksgA

Synonym: ACIAD3009

Alternate gene names: 50086027

Gene position: 2937710-2936886 (Counterclockwise)

Preceding gene: 50086028

Following gene: 50086026

Centisome position: 81.63

GC content: 46.06

Gene sequence:

>825_bases
TTGGAAGTCTTTATGTATCAAATTAATGCCCTAAACCCCAAAGAAGAAGGGCATAAAGCTCGGAAACGTTTTGGTCAGAA
CTTTCTACACGACCAACGGGTTATTGCGAAAATCGTCCGCTCGGTCAATCCGCGCCCAGGTGATAATGTTGTCGAAATCG
GACCTGGTCTGGCAGCACTGACTTCACCTTTGATTGGTGAGTGTGATGCCCTAACTGTGGTCGAACTTGACCGCGACTTG
GCGGCAGGTTTGCCTGATCGCGTACCGCATCCAGAGCGTTTAACCATTGTTGAAGCCGATGCGCTTAAATATGATTTTAG
CCAGTTAGCGACGCAAGAGAGTCCACTTCGCGTGGTAGGTAACTTACCATATAACATCTCAACGCCTTTGTTGTTTCATC
TGTTGGAATTTGGCGACAAAGTCAAAGATATGCATTTTATGCTACAAAAAGAAGTGGTTGATCGTATCACTGCCGAGCCT
AATACCAAAGAATATGGTCGTTTGTCGGTCATGATTCAGTATTTCTGTCAGCCCACATTTTTATTTGAGGTGCCTGCTGG
CGCATTTAATCCACCTCCAAAAGTCACAAGTGCGGTATTTCGTTTAGTGCCGTATAAAGAAAAACCGATTGTGGCCAAAG
ATGAAAAAGCATTGTCTCGTCTGGTCGGGCATGTGTTTACGCAGCGTCGTAAAACCTTGCGAAATAGCCTGAAGGGCATG
CTTGCAGACGATGCTTTTGACAAAGCGGGTATCGATCCGATGGCACGCCCAGAAACCTTAACGCTGGCACAATTTGTGGC
ATTGTCTGATCAAATGGTGCCATAA

Upstream 100 bases:

>100_bases
ACCGCGCTGTCTCTGGCTGGAACTGGATTGGCAAAAAGTACAAGTTTACATGTGGCGGTTGAGCTGGCTTTGGCGCTGGC
ACGTCAATAATCGAATTACG

Downstream 100 bases:

>100_bases
GTATGACCACACGCTACAACTATGTGATTGGTGATGTTCAGGGATGTTTTGAAGCGCTCAAAGCATTGCTGAAAGAAATT
CGGTTTGATCCTGATTTAGA

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MEVFMYQINALNPKEEGHKARKRFGQNFLHDQRVIAKIVRSVNPRPGDNVVEIGPGLAALTSPLIGECDALTVVELDRDL
AAGLPDRVPHPERLTIVEADALKYDFSQLATQESPLRVVGNLPYNISTPLLFHLLEFGDKVKDMHFMLQKEVVDRITAEP
NTKEYGRLSVMIQYFCQPTFLFEVPAGAFNPPPKVTSAVFRLVPYKEKPIVAKDEKALSRLVGHVFTQRRKTLRNSLKGM
LADDAFDKAGIDPMARPETLTLAQFVALSDQMVP

Sequences:

>Translated_274_residues
MEVFMYQINALNPKEEGHKARKRFGQNFLHDQRVIAKIVRSVNPRPGDNVVEIGPGLAALTSPLIGECDALTVVELDRDL
AAGLPDRVPHPERLTIVEADALKYDFSQLATQESPLRVVGNLPYNISTPLLFHLLEFGDKVKDMHFMLQKEVVDRITAEP
NTKEYGRLSVMIQYFCQPTFLFEVPAGAFNPPPKVTSAVFRLVPYKEKPIVAKDEKALSRLVGHVFTQRRKTLRNSLKGM
LADDAFDKAGIDPMARPETLTLAQFVALSDQMVP
>Mature_274_residues
MEVFMYQINALNPKEEGHKARKRFGQNFLHDQRVIAKIVRSVNPRPGDNVVEIGPGLAALTSPLIGECDALTVVELDRDL
AAGLPDRVPHPERLTIVEADALKYDFSQLATQESPLRVVGNLPYNISTPLLFHLLEFGDKVKDMHFMLQKEVVDRITAEP
NTKEYGRLSVMIQYFCQPTFLFEVPAGAFNPPPKVTSAVFRLVPYKEKPIVAKDEKALSRLVGHVFTQRRKTLRNSLKGM
LADDAFDKAGIDPMARPETLTLAQFVALSDQMVP

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI156415992, Length=283, Percent_Identity=33.9222614840989, Blast_Score=121, Evalue=8e-28,
Organism=Homo sapiens, GI7657198, Length=247, Percent_Identity=31.5789473684211, Blast_Score=105, Evalue=4e-23,
Organism=Escherichia coli, GI1786236, Length=262, Percent_Identity=53.8167938931298, Blast_Score=282, Evalue=2e-77,
Organism=Caenorhabditis elegans, GI25141369, Length=286, Percent_Identity=27.6223776223776, Blast_Score=104, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI25146882, Length=213, Percent_Identity=33.3333333333333, Blast_Score=97, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6324989, Length=253, Percent_Identity=29.2490118577075, Blast_Score=100, Evalue=4e-22,
Organism=Drosophila melanogaster, GI21357273, Length=277, Percent_Identity=31.0469314079422, Blast_Score=108, Evalue=4e-24,
Organism=Drosophila melanogaster, GI21358017, Length=231, Percent_Identity=33.3333333333333, Blast_Score=103, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_ACIAD (Q6F8A0)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_047537.1
- ProteinModelPortal:   Q6F8A0
- SMR:   Q6F8A0
- STRING:   Q6F8A0
- GeneID:   2880109
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD3009
- NMPDR:   fig|62977.3.peg.2730
- eggNOG:   COG0030
- HOGENOM:   HBG319664
- OMA:   GVLCGWR
- PhylomeDB:   Q6F8A0
- ProtClustDB:   PRK00274
- BioCyc:   ASP62977:ACIAD3009-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.182

Molecular weight: Translated: 30693; Mature: 30693

Theoretical pI: Translated: 7.67; Mature: 7.67

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 27-27 BINDING 29-29 BINDING 54-54 BINDING 75-75 BINDING 100-100 BINDING 121-121

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVFMYQINALNPKEEGHKARKRFGQNFLHDQRVIAKIVRSVNPRPGDNVVEIGPGLAAL
CEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHH
TSPLIGECDALTVVELDRDLAAGLPDRVPHPERLTIVEADALKYDFSQLATQESPLRVVG
HCCCCCCCCCEEEEEECHHHHCCCCCCCCCCCEEEEEEHHHHHHHHHHHHCCCCCEEEEC
NLPYNISTPLLFHLLEFGDKVKDMHFMLQKEVVDRITAEPNTKEYGRLSVMIQYFCQPTF
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEE
LFEVPAGAFNPPPKVTSAVFRLVPYKEKPIVAKDEKALSRLVGHVFTQRRKTLRNSLKGM
EEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LADDAFDKAGIDPMARPETLTLAQFVALSDQMVP
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MEVFMYQINALNPKEEGHKARKRFGQNFLHDQRVIAKIVRSVNPRPGDNVVEIGPGLAAL
CEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHH
TSPLIGECDALTVVELDRDLAAGLPDRVPHPERLTIVEADALKYDFSQLATQESPLRVVG
HCCCCCCCCCEEEEEECHHHHCCCCCCCCCCCEEEEEEHHHHHHHHHHHHCCCCCEEEEC
NLPYNISTPLLFHLLEFGDKVKDMHFMLQKEVVDRITAEPNTKEYGRLSVMIQYFCQPTF
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEE
LFEVPAGAFNPPPKVTSAVFRLVPYKEKPIVAKDEKALSRLVGHVFTQRRKTLRNSLKGM
EEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LADDAFDKAGIDPMARPETLTLAQFVALSDQMVP
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA