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The map label for this gene is lpd [H]

Identifier: 50085914

GI number: 50085914

Start: 2814788

End: 2816221

Strand: Reverse

Name: lpd [H]

Synonym: ACIAD2874

Alternate gene names: 50085914

Gene position: 2816221-2814788 (Counterclockwise)

Preceding gene: 50085915

Following gene: 50085913

Centisome position: 78.26

GC content: 43.03

Gene sequence:

>1434_bases
ATGTCTCAACAATTTGATCTTGTTGTAATTGGCGGTGGACCAGGCGGTTATGAAGCGGCGATTCGTGCTGCTCAGCTTGG
TTTTAAAGTTGCTTGTATTGAGAAACGTGTTCATAAAGGTAAACCATCTTTAGGTGGTACGTGCTTAAACGTTGGATGTA
TTCCTTCTAAAGCACTCCTTGATTCGTCTCATCATTATGAAGATACGATTCATCATCTTGGTGATCATGGTATTACAACA
GGCGAAGTTAATATTGACATCGCAAAAATGTTGGCACGCAAAGATAAAGTTGTAGAGAATTTGACTGGTGGTGTTGCCCA
ATTATTAAAAGGCAATGGCATTGAGTGGTTGCAAGGTACGGGTAAGTTACTTGCGGGTAAGAAAGTTGAGTTTGTTCCTT
TCGAAGGTGAAACTCAGGTTTTAGAACCTAAATATGTGATCCTCGCTTCTGGTTCTGTTCCAGTCAATATCCCAGTTGCC
CCTGTTGATCAAGACTTGATTGTTGACTCAACTGGTGCACTCGAGTTTGCTGAAGTACCTAAGCGTTTGGGTGTGATTGG
TGCAGGTGTGATTGGTCTGGAACTTGGTTCTGTATGGCGCCGTTTGGGTTCTGAAGTGGTTGTATTTGAAGCATTGGATG
CATTCTTGCCAATGGCAGATAAAGCACTTTCAAAAGAATTCCAAAAAATTCTGACCAAGCAAGGCCTTGATATTCGCATT
GGTGCTAAAGTTTCTGGTACTGAGGTGAATGGTCGTGAAGTGACGGTTAAATACAATCAGGCTGGCGAAGACAAAGAACA
AGTGTTTGACAAGTTGATCGTATGCGTAGGTCGTAAGCCTTATGCAGAAGGTTTGTTGGCAGAAGATGCTGGCATTAAAC
TGACTGATCGTGGCTTTATCGAAGTAAACGACCATTGTGCAACTTCTCTTGAAGGTGTGTATGCGATTGGTGACCTTGTC
CGTGGTCCTATGCTTGCGCATAAAGCCATGGAAGAAGGTGTGATGGCAGTTGAGCGTATTCACGGTCATGCAGCACAAGT
GAACTACGATACAATTATCAGCGTTATTTACACGCACCCTGAAGCAGCTTGGGTTGGCTTAACTGAAGAGCAAGCTGTTG
CAAATGGTCATGAAGTAAAAACAGGTCAATTCCCATTTGCCGTGAATGGTCGTGCTCTTGCTGCTGGTGAAGCTGCTGGT
TTTGTAAAATTCGTTGCTGATGCTAAAACTGATCGCTTGTTAGGAATGCATGTGATTGGTCCTGGTGCTTCAGATATTGT
ACATCAAGGGATGATTGCACTTGAGTTTGTATCTTCTGTAGAAGATTTACAATTAATGACATTTGGCCATCCGACTTATT
CTGAGGTGGTTCATGAGGCTGCTTTAGCTGTTGATGGTCGTGCAATTCATGCGATCCAGCGTAAACGTAAATAA

Upstream 100 bases:

>100_bases
AGAGCTTCTAGAAGAGCCTGCAAAACTTATTCTTGATCTTTAATTCATTCCGGACAATAGCCCAGATTGAGATGATTCAT
CTGGGTTTATGGAGATAAAA

Downstream 100 bases:

>100_bases
TTGAAAAAAGAGCGGTGTAAACCGCTCTTTTTTGTTTAAGGTGGTTGTTTATATCTGAATAATCATCTAAAAATAAGTAC
AAACGAAATTGAAAAAATGA

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: E3 component of 2-oxoglutarate dehydrogenase complex; Glycine oxidation system L-factor; LPD-GLC [H]

Number of amino acids: Translated: 477; Mature: 476

Protein sequence:

>477_residues
MSQQFDLVVIGGGPGGYEAAIRAAQLGFKVACIEKRVHKGKPSLGGTCLNVGCIPSKALLDSSHHYEDTIHHLGDHGITT
GEVNIDIAKMLARKDKVVENLTGGVAQLLKGNGIEWLQGTGKLLAGKKVEFVPFEGETQVLEPKYVILASGSVPVNIPVA
PVDQDLIVDSTGALEFAEVPKRLGVIGAGVIGLELGSVWRRLGSEVVVFEALDAFLPMADKALSKEFQKILTKQGLDIRI
GAKVSGTEVNGREVTVKYNQAGEDKEQVFDKLIVCVGRKPYAEGLLAEDAGIKLTDRGFIEVNDHCATSLEGVYAIGDLV
RGPMLAHKAMEEGVMAVERIHGHAAQVNYDTIISVIYTHPEAAWVGLTEEQAVANGHEVKTGQFPFAVNGRALAAGEAAG
FVKFVADAKTDRLLGMHVIGPGASDIVHQGMIALEFVSSVEDLQLMTFGHPTYSEVVHEAALAVDGRAIHAIQRKRK

Sequences:

>Translated_477_residues
MSQQFDLVVIGGGPGGYEAAIRAAQLGFKVACIEKRVHKGKPSLGGTCLNVGCIPSKALLDSSHHYEDTIHHLGDHGITT
GEVNIDIAKMLARKDKVVENLTGGVAQLLKGNGIEWLQGTGKLLAGKKVEFVPFEGETQVLEPKYVILASGSVPVNIPVA
PVDQDLIVDSTGALEFAEVPKRLGVIGAGVIGLELGSVWRRLGSEVVVFEALDAFLPMADKALSKEFQKILTKQGLDIRI
GAKVSGTEVNGREVTVKYNQAGEDKEQVFDKLIVCVGRKPYAEGLLAEDAGIKLTDRGFIEVNDHCATSLEGVYAIGDLV
RGPMLAHKAMEEGVMAVERIHGHAAQVNYDTIISVIYTHPEAAWVGLTEEQAVANGHEVKTGQFPFAVNGRALAAGEAAG
FVKFVADAKTDRLLGMHVIGPGASDIVHQGMIALEFVSSVEDLQLMTFGHPTYSEVVHEAALAVDGRAIHAIQRKRK
>Mature_476_residues
SQQFDLVVIGGGPGGYEAAIRAAQLGFKVACIEKRVHKGKPSLGGTCLNVGCIPSKALLDSSHHYEDTIHHLGDHGITTG
EVNIDIAKMLARKDKVVENLTGGVAQLLKGNGIEWLQGTGKLLAGKKVEFVPFEGETQVLEPKYVILASGSVPVNIPVAP
VDQDLIVDSTGALEFAEVPKRLGVIGAGVIGLELGSVWRRLGSEVVVFEALDAFLPMADKALSKEFQKILTKQGLDIRIG
AKVSGTEVNGREVTVKYNQAGEDKEQVFDKLIVCVGRKPYAEGLLAEDAGIKLTDRGFIEVNDHCATSLEGVYAIGDLVR
GPMLAHKAMEEGVMAVERIHGHAAQVNYDTIISVIYTHPEAAWVGLTEEQAVANGHEVKTGQFPFAVNGRALAAGEAAGF
VKFVADAKTDRLLGMHVIGPGASDIVHQGMIALEFVSSVEDLQLMTFGHPTYSEVVHEAALAVDGRAIHAIQRKRK

Specific function: Also acts in the glycine cleavage system [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=473, Percent_Identity=45.6659619450317, Blast_Score=402, Evalue=1e-112,
Organism=Homo sapiens, GI50301238, Length=467, Percent_Identity=27.1948608137045, Blast_Score=148, Evalue=9e-36,
Organism=Homo sapiens, GI22035672, Length=459, Percent_Identity=26.797385620915, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI33519430, Length=476, Percent_Identity=23.3193277310924, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI33519428, Length=476, Percent_Identity=23.3193277310924, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI33519426, Length=476, Percent_Identity=23.3193277310924, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI148277065, Length=476, Percent_Identity=23.3193277310924, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI148277071, Length=476, Percent_Identity=23.3193277310924, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI291045266, Length=463, Percent_Identity=24.4060475161987, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI291045268, Length=455, Percent_Identity=23.0769230769231, Blast_Score=83, Evalue=5e-16,
Organism=Escherichia coli, GI1786307, Length=462, Percent_Identity=39.6103896103896, Blast_Score=308, Evalue=4e-85,
Organism=Escherichia coli, GI87081717, Length=461, Percent_Identity=26.2472885032538, Blast_Score=161, Evalue=8e-41,
Organism=Escherichia coli, GI87082354, Length=472, Percent_Identity=28.3898305084746, Blast_Score=158, Evalue=8e-40,
Organism=Escherichia coli, GI1789915, Length=460, Percent_Identity=26.9565217391304, Blast_Score=154, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI32565766, Length=475, Percent_Identity=45.0526315789474, Blast_Score=402, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI17557007, Length=476, Percent_Identity=27.1008403361345, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI71983419, Length=378, Percent_Identity=29.6296296296296, Blast_Score=124, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI71983429, Length=378, Percent_Identity=29.6296296296296, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI71982272, Length=475, Percent_Identity=23.1578947368421, Blast_Score=94, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6321091, Length=485, Percent_Identity=43.5051546391753, Blast_Score=369, Evalue=1e-103,
Organism=Saccharomyces cerevisiae, GI6325240, Length=472, Percent_Identity=28.8135593220339, Blast_Score=190, Evalue=5e-49,
Organism=Saccharomyces cerevisiae, GI6325166, Length=469, Percent_Identity=26.4392324093817, Blast_Score=154, Evalue=3e-38,
Organism=Drosophila melanogaster, GI21358499, Length=468, Percent_Identity=47.008547008547, Blast_Score=418, Evalue=1e-117,
Organism=Drosophila melanogaster, GI17737741, Length=472, Percent_Identity=28.3898305084746, Blast_Score=142, Evalue=5e-34,
Organism=Drosophila melanogaster, GI24640553, Length=474, Percent_Identity=27.6371308016878, Blast_Score=135, Evalue=9e-32,
Organism=Drosophila melanogaster, GI24640551, Length=475, Percent_Identity=28, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24640549, Length=474, Percent_Identity=27.6371308016878, Blast_Score=134, Evalue=1e-31,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 50910; Mature: 50779

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: PS00076 PYRIDINE_REDOX_1 ; PS00098 THIOLASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQQFDLVVIGGGPGGYEAAIRAAQLGFKVACIEKRVHKGKPSLGGTCLNVGCIPSKALL
CCCCEEEEEEECCCCCHHHHHHHHHHCEEEEHHHHHHHCCCCCCCCEEEEECCCCCHHHH
DSSHHYEDTIHHLGDHGITTGEVNIDIAKMLARKDKVVENLTGGVAQLLKGNGIEWLQGT
CCCCCHHHHHHHCCCCCCEECEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCC
GKLLAGKKVEFVPFEGETQVLEPKYVILASGSVPVNIPVAPVDQDLIVDSTGALEFAEVP
CCEECCCEEEEEECCCCCEEECCCEEEEECCCCEEEEECCCCCCCEEEECCCCCHHHHHH
KRLGVIGAGVIGLELGSVWRRLGSEVVVFEALDAFLPMADKALSKEFQKILTKQGLDIRI
HHHCHHCCCHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
GAKVSGTEVNGREVTVKYNQAGEDKEQVFDKLIVCVGRKPYAEGLLAEDAGIKLTDRGFI
CCEEECCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCHHCCCEECCCCCEEECCCEE
EVNDHCATSLEGVYAIGDLVRGPMLAHKAMEEGVMAVERIHGHAAQVNYDTIISVIYTHP
EECCCHHHHHCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCEEECHHHEEEEEEECC
EAAWVGLTEEQAVANGHEVKTGQFPFAVNGRALAAGEAAGFVKFVADAKTDRLLGMHVIG
CEEEECCCHHHHHCCCCEEECCCCCEEECCCEEECCCHHHHHHHHHCCCCCCEEEEEEEC
PGASDIVHQGMIALEFVSSVEDLQLMTFGHPTYSEVVHEAALAVDGRAIHAIQRKRK
CCHHHHHHHHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHCCC
>Mature Secondary Structure 
SQQFDLVVIGGGPGGYEAAIRAAQLGFKVACIEKRVHKGKPSLGGTCLNVGCIPSKALL
CCCEEEEEEECCCCCHHHHHHHHHHCEEEEHHHHHHHCCCCCCCCEEEEECCCCCHHHH
DSSHHYEDTIHHLGDHGITTGEVNIDIAKMLARKDKVVENLTGGVAQLLKGNGIEWLQGT
CCCCCHHHHHHHCCCCCCEECEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCC
GKLLAGKKVEFVPFEGETQVLEPKYVILASGSVPVNIPVAPVDQDLIVDSTGALEFAEVP
CCEECCCEEEEEECCCCCEEECCCEEEEECCCCEEEEECCCCCCCEEEECCCCCHHHHHH
KRLGVIGAGVIGLELGSVWRRLGSEVVVFEALDAFLPMADKALSKEFQKILTKQGLDIRI
HHHCHHCCCHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
GAKVSGTEVNGREVTVKYNQAGEDKEQVFDKLIVCVGRKPYAEGLLAEDAGIKLTDRGFI
CCEEECCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCHHCCCEECCCCCEEECCCEE
EVNDHCATSLEGVYAIGDLVRGPMLAHKAMEEGVMAVERIHGHAAQVNYDTIISVIYTHP
EECCCHHHHHCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCEEECHHHEEEEEEECC
EAAWVGLTEEQAVANGHEVKTGQFPFAVNGRALAAGEAAGFVKFVADAKTDRLLGMHVIG
CEEEECCCHHHHHCCCCEEECCCCCEEECCCEEECCCHHHHHHHHHCCCCCCEEEEEEEC
PGASDIVHQGMIALEFVSSVEDLQLMTFGHPTYSEVVHEAALAVDGRAIHAIQRKRK
CCHHHHHHHHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1902462; 2914869 [H]