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The map label for this gene is sucB [H]

Identifier: 50085915

GI number: 50085915

Start: 2816279

End: 2817487

Strand: Reverse

Name: sucB [H]

Synonym: ACIAD2875

Alternate gene names: 50085915

Gene position: 2817487-2816279 (Counterclockwise)

Preceding gene: 50085916

Following gene: 50085914

Centisome position: 78.29

GC content: 43.18

Gene sequence:

>1209_bases
ATGGCAACCGAAATTAAAGCACCGGTATTCCCAGAGTCAGTTGCAGATGGAACCATTGCAACATGGCATAAGAAAGTTGG
GGAAGCAGTATCACGTGATGAAGTCATTTGTGATATTGAAACTGATAAAGTTGTTTTAGAAGTGGTTGCTCCAGCTGATG
GTCAACTTGTTTCTATTATCAAAGACGAAGGCGAAACAGTTTTATCTGACGAAGTGATTGCACAATTCCAAGAAGGTGCA
GTGTCAGGTGCAAATGAAACTCAAGCTGTTCAAAGCGACTCTAAAGTAGAGCAGGCTGCGGCTAAAACTGAAGCGGGTGC
AGCTCCAGTTGTTGAGCGTGCGCAACCCGTTTCTGATCAAGCTCCAGCAGTTCGCAAGGCTTTGACTGAGTCTGGTATCA
GTGCATCAGACGTTCAAGGTACTGGTCGTGGTGGTCGTATCACCAAAGAAGATGTCGTCAATCATCAATCTAAACCAGCT
GCACAACCACTGAGTGTTGCAGTGGGTGAGCGTATCGAAAAACGTGTTCCAATGACACGTCTTCGTAAGCGTGTTGCTGA
ACGTCTTCTTGCTGCCACTCAAGAAACAGCAATGTTGACAACGTTTAACGAAGTCAACATGAAACCAATCATGGAAATGC
GTGCACAGTATAAAGATGCATTTGAAAAACGTCATGGTGCGCGTCTTGGCTTTATGTCTTTCTTTGTTAAAGCAGCAACT
GAAGCATTGAAGCGTTATCCTGCGGTGAATGCATCTATCGATGGCGATGACATCGTTTATCACGGTTTCTACGATATTGG
TGTAGCTGTATCTTCTGATCGTGGTCTCGTGGTTCCAGTACTACGTGATACTGATCGCATGAACTATGCTGAAGTTGAAA
ATGGTATCCGTGACTATGCTGTCAAAGCGCGTGATGGCAAGTTGGGTATTGAAGATATGACTGGCGGTACATTTACCATC
ACCAATGGTGGTACATTCGGTTCATTGCTTTCTACTCCAATTTTAAATACACCACAAACTGCAATCTTGGGTATGCATAA
AATTCAAGAACGTCCAATGGCAGTGAATGGTCAGGTTGAAATTCTTCCAATGATGTATTTGGCTCTTTCTTATGACCATC
GCTTAATTGATGGTAAAGAAGCAGTTGGCTTCTTGGTTACTATTAAAGAGCTTCTAGAAGAGCCTGCAAAACTTATTCTT
GATCTTTAA

Upstream 100 bases:

>100_bases
CACCAGCGTGTGGCTCACCATATTTGCATGCAAAACAACAAGCTCAGCTCATTCATGATGCATTAGCAATCGAAGCTGAA
CAATCAGGAGAGACAAAATA

Downstream 100 bases:

>100_bases
TTCATTCCGGACAATAGCCCAGATTGAGATGATTCATCTGGGTTTATGGAGATAAAAATGTCTCAACAATTTGATCTTGT
TGTAATTGGCGGTGGACCAG

Product: dihydrolipoamide succinyltransferase, component of 2-oxoglutarate dehydrogenase complex (E2)

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 402; Mature: 401

Protein sequence:

>402_residues
MATEIKAPVFPESVADGTIATWHKKVGEAVSRDEVICDIETDKVVLEVVAPADGQLVSIIKDEGETVLSDEVIAQFQEGA
VSGANETQAVQSDSKVEQAAAKTEAGAAPVVERAQPVSDQAPAVRKALTESGISASDVQGTGRGGRITKEDVVNHQSKPA
AQPLSVAVGERIEKRVPMTRLRKRVAERLLAATQETAMLTTFNEVNMKPIMEMRAQYKDAFEKRHGARLGFMSFFVKAAT
EALKRYPAVNASIDGDDIVYHGFYDIGVAVSSDRGLVVPVLRDTDRMNYAEVENGIRDYAVKARDGKLGIEDMTGGTFTI
TNGGTFGSLLSTPILNTPQTAILGMHKIQERPMAVNGQVEILPMMYLALSYDHRLIDGKEAVGFLVTIKELLEEPAKLIL
DL

Sequences:

>Translated_402_residues
MATEIKAPVFPESVADGTIATWHKKVGEAVSRDEVICDIETDKVVLEVVAPADGQLVSIIKDEGETVLSDEVIAQFQEGA
VSGANETQAVQSDSKVEQAAAKTEAGAAPVVERAQPVSDQAPAVRKALTESGISASDVQGTGRGGRITKEDVVNHQSKPA
AQPLSVAVGERIEKRVPMTRLRKRVAERLLAATQETAMLTTFNEVNMKPIMEMRAQYKDAFEKRHGARLGFMSFFVKAAT
EALKRYPAVNASIDGDDIVYHGFYDIGVAVSSDRGLVVPVLRDTDRMNYAEVENGIRDYAVKARDGKLGIEDMTGGTFTI
TNGGTFGSLLSTPILNTPQTAILGMHKIQERPMAVNGQVEILPMMYLALSYDHRLIDGKEAVGFLVTIKELLEEPAKLIL
DL
>Mature_401_residues
ATEIKAPVFPESVADGTIATWHKKVGEAVSRDEVICDIETDKVVLEVVAPADGQLVSIIKDEGETVLSDEVIAQFQEGAV
SGANETQAVQSDSKVEQAAAKTEAGAAPVVERAQPVSDQAPAVRKALTESGISASDVQGTGRGGRITKEDVVNHQSKPAA
QPLSVAVGERIEKRVPMTRLRKRVAERLLAATQETAMLTTFNEVNMKPIMEMRAQYKDAFEKRHGARLGFMSFFVKAATE
ALKRYPAVNASIDGDDIVYHGFYDIGVAVSSDRGLVVPVLRDTDRMNYAEVENGIRDYAVKARDGKLGIEDMTGGTFTIT
NGGTFGSLLSTPILNTPQTAILGMHKIQERPMAVNGQVEILPMMYLALSYDHRLIDGKEAVGFLVTIKELLEEPAKLILD
L

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=246, Percent_Identity=56.0975609756098, Blast_Score=280, Evalue=2e-75,
Organism=Homo sapiens, GI110671329, Length=424, Percent_Identity=26.6509433962264, Blast_Score=153, Evalue=2e-37,
Organism=Homo sapiens, GI31711992, Length=438, Percent_Identity=26.7123287671233, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI203098753, Length=465, Percent_Identity=26.4516129032258, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI203098816, Length=465, Percent_Identity=26.4516129032258, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI260898739, Length=161, Percent_Identity=34.1614906832298, Blast_Score=95, Evalue=9e-20,
Organism=Escherichia coli, GI1786946, Length=416, Percent_Identity=59.6153846153846, Blast_Score=497, Evalue=1e-142,
Organism=Escherichia coli, GI1786305, Length=407, Percent_Identity=29.2383292383292, Blast_Score=141, Evalue=9e-35,
Organism=Caenorhabditis elegans, GI25146366, Length=405, Percent_Identity=43.2098765432099, Blast_Score=315, Evalue=2e-86,
Organism=Caenorhabditis elegans, GI17560088, Length=426, Percent_Identity=27.9342723004695, Blast_Score=152, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17537937, Length=414, Percent_Identity=25.8454106280193, Blast_Score=149, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17538894, Length=305, Percent_Identity=29.5081967213115, Blast_Score=118, Evalue=5e-27,
Organism=Saccharomyces cerevisiae, GI6320352, Length=402, Percent_Identity=43.0348258706468, Blast_Score=322, Evalue=9e-89,
Organism=Saccharomyces cerevisiae, GI6324258, Length=456, Percent_Identity=26.5350877192982, Blast_Score=142, Evalue=8e-35,
Organism=Drosophila melanogaster, GI24645909, Length=235, Percent_Identity=56.5957446808511, Blast_Score=273, Evalue=2e-73,
Organism=Drosophila melanogaster, GI18859875, Length=416, Percent_Identity=26.6826923076923, Blast_Score=144, Evalue=8e-35,
Organism=Drosophila melanogaster, GI24582497, Length=229, Percent_Identity=29.6943231441048, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI20129315, Length=229, Percent_Identity=29.6943231441048, Blast_Score=120, Evalue=2e-27,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 43482; Mature: 43351

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATEIKAPVFPESVADGTIATWHKKVGEAVSRDEVICDIETDKVVLEVVAPADGQLVSII
CCCCCCCCCCCCHHCCCCHHHHHHHHHHHHCCCCEEEEECCCEEEEEEECCCCCCEEEEE
KDEGETVLSDEVIAQFQEGAVSGANETQAVQSDSKVEQAAAKTEAGAAPVVERAQPVSDQ
CCCCCEEHHHHHHHHHHHCCCCCCCHHHHHHCHHHHHHHHHHHCCCCCCHHHHCCCCCCC
APAVRKALTESGISASDVQGTGRGGRITKEDVVNHQSKPAAQPLSVAVGERIEKRVPMTR
CHHHHHHHHHCCCCHHCCCCCCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHCCCHHH
LRKRVAERLLAATQETAMLTTFNEVNMKPIMEMRAQYKDAFEKRHGARLGFMSFFVKAAT
HHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
EALKRYPAVNASIDGDDIVYHGFYDIGVAVSSDRGLVVPVLRDTDRMNYAEVENGIRDYA
HHHHHCCCCCCCCCCCCEEEECEEEEEEEEECCCCEEEEEECCCCCCCHHHHHCCHHHHE
VKARDGKLGIEDMTGGTFTITNGGTFGSLLSTPILNTPQTAILGMHKIQERPMAVNGQVE
EECCCCCCCCEECCCCEEEEECCCCHHHHHHCCCCCCCHHHHHHHHHHHCCCCEECCCEE
ILPMMYLALSYDHRLIDGKEAVGFLVTIKELLEEPAKLILDL
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ATEIKAPVFPESVADGTIATWHKKVGEAVSRDEVICDIETDKVVLEVVAPADGQLVSII
CCCCCCCCCCCHHCCCCHHHHHHHHHHHHCCCCEEEEECCCEEEEEEECCCCCCEEEEE
KDEGETVLSDEVIAQFQEGAVSGANETQAVQSDSKVEQAAAKTEAGAAPVVERAQPVSDQ
CCCCCEEHHHHHHHHHHHCCCCCCCHHHHHHCHHHHHHHHHHHCCCCCCHHHHCCCCCCC
APAVRKALTESGISASDVQGTGRGGRITKEDVVNHQSKPAAQPLSVAVGERIEKRVPMTR
CHHHHHHHHHCCCCHHCCCCCCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHCCCHHH
LRKRVAERLLAATQETAMLTTFNEVNMKPIMEMRAQYKDAFEKRHGARLGFMSFFVKAAT
HHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
EALKRYPAVNASIDGDDIVYHGFYDIGVAVSSDRGLVVPVLRDTDRMNYAEVENGIRDYA
HHHHHCCCCCCCCCCCCEEEECEEEEEEEEECCCCEEEEEECCCCCCCHHHHHCCHHHHE
VKARDGKLGIEDMTGGTFTITNGGTFGSLLSTPILNTPQTAILGMHKIQERPMAVNGQVE
EECCCCCCCCEECCCCEEEEECCCCHHHHHHCCCCCCCHHHHHHHHHHHCCCCEECCCEE
ILPMMYLALSYDHRLIDGKEAVGFLVTIKELLEEPAKLILDL
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]