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The map label for this gene is pdxB

Identifier: 50085734

GI number: 50085734

Start: 2621280

End: 2622347

Strand: Reverse

Name: pdxB

Synonym: ACIAD2667

Alternate gene names: 50085734

Gene position: 2622347-2621280 (Counterclockwise)

Preceding gene: 50085735

Following gene: 50085733

Centisome position: 72.87

GC content: 42.51

Gene sequence:

>1068_bases
ATGAAAATTGTCGCAGATGAAAATCTTGCGTTTACAGATTACTTTTTCTCAGAATTTGGAGAAATTCAACATCGGGCAGG
ACGTCTTTTGACTGCGCATGATGTGAGTGATGCTGAAGCCTTATTGGTTCGTTCAGTGACCAAAGTGAATCAGGCACTGA
TTGAACACAGTCAGTTGAAATTTGTAGGCAGTGCTACAATTGGAACAGATCATCTTGATATTTCAGCATTGCAACAGCAA
GATATTTTATGGAGCAATGCACCTGGCTGTAATGCGCAGGCGGTTGCAGAATATGTCATCACGGCATTATATCATCTTGA
TTCGGATGTATTTGAGCGGGGACAGGATTTTACCTTAGGGATTATTGGACTGGGAAATGTAGGACGCCGACTTGCGAAGA
TGGCAGCGCTGTTAGGGTGGAATGTCATTGGTTGTGATCCTTTCGTTCAGTTACCAGACATTCATAATCTGAGCTTTGAT
GATGTGCTCCAAAAAAGTGATGCAATTTCGGTTCATGTTCCATTGACTCACTCAGGTTCACATCCGACCTTCCATTTGTT
CGATCAACACGCTTTTGCATCTATGCCTGCAAGCACTATTTTGATCAATAGTGCGCGCGGCCCAGTGATTGAGGAGCAAG
CCCTGATTCAAGATATCTACCAAACTGGACGTAAAGTGGTGCTGGATGTATTCGAGCATGAACCTGTAATTTCCGAACAA
TTACTGGATGTAGTGAATTTAGTCACGCCGCATATTGCAGGTTATAGTTTGGAGGGAAAGGCACGTGGAACCCAGATGAT
CTATGATGCCTTTTGTAAAGTATTTGGCTATGAAGCCAGTAAAAAATTTGAAACACAATTGCCAGTTTGTGAACCTTTTT
TTCAACAACAGGATTTAAAGCAAATACTTAAAGCTCATTTACGTGCTATTTATGATATTGCACAAGATGATCACAATTTA
CGTGCTTGTGTAAAAGATGGACAAGTTGATCAGTGTGCTTTTGATCAGCTTCGAAAGGAATACCCGCTTCGCCGTGAATG
GGCAGCGCATGGAGGCCCTGTCGCGTGA

Upstream 100 bases:

>100_bases
TTAAGTATGTTGCAATCCAAATCTGTAAATTTCAGAATTTACATGGTCACCTAAATTTTCACGCGTTATCATAGCGATGT
TGAAATCATGAGGCTGGACT

Downstream 100 bases:

>100_bases
ATTTAGAGCATCACTCAAATCATTATCAACCTACGTGTTCTATCGAGGCACTTCAAGCCCGTGCCAAGCTTTACCAAACT
ATTCGCACATTTTTTGCAGA

Product: erythronate-4-phosphate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MKIVADENLAFTDYFFSEFGEIQHRAGRLLTAHDVSDAEALLVRSVTKVNQALIEHSQLKFVGSATIGTDHLDISALQQQ
DILWSNAPGCNAQAVAEYVITALYHLDSDVFERGQDFTLGIIGLGNVGRRLAKMAALLGWNVIGCDPFVQLPDIHNLSFD
DVLQKSDAISVHVPLTHSGSHPTFHLFDQHAFASMPASTILINSARGPVIEEQALIQDIYQTGRKVVLDVFEHEPVISEQ
LLDVVNLVTPHIAGYSLEGKARGTQMIYDAFCKVFGYEASKKFETQLPVCEPFFQQQDLKQILKAHLRAIYDIAQDDHNL
RACVKDGQVDQCAFDQLRKEYPLRREWAAHGGPVA

Sequences:

>Translated_355_residues
MKIVADENLAFTDYFFSEFGEIQHRAGRLLTAHDVSDAEALLVRSVTKVNQALIEHSQLKFVGSATIGTDHLDISALQQQ
DILWSNAPGCNAQAVAEYVITALYHLDSDVFERGQDFTLGIIGLGNVGRRLAKMAALLGWNVIGCDPFVQLPDIHNLSFD
DVLQKSDAISVHVPLTHSGSHPTFHLFDQHAFASMPASTILINSARGPVIEEQALIQDIYQTGRKVVLDVFEHEPVISEQ
LLDVVNLVTPHIAGYSLEGKARGTQMIYDAFCKVFGYEASKKFETQLPVCEPFFQQQDLKQILKAHLRAIYDIAQDDHNL
RACVKDGQVDQCAFDQLRKEYPLRREWAAHGGPVA
>Mature_355_residues
MKIVADENLAFTDYFFSEFGEIQHRAGRLLTAHDVSDAEALLVRSVTKVNQALIEHSQLKFVGSATIGTDHLDISALQQQ
DILWSNAPGCNAQAVAEYVITALYHLDSDVFERGQDFTLGIIGLGNVGRRLAKMAALLGWNVIGCDPFVQLPDIHNLSFD
DVLQKSDAISVHVPLTHSGSHPTFHLFDQHAFASMPASTILINSARGPVIEEQALIQDIYQTGRKVVLDVFEHEPVISEQ
LLDVVNLVTPHIAGYSLEGKARGTQMIYDAFCKVFGYEASKKFETQLPVCEPFFQQQDLKQILKAHLRAIYDIAQDDHNL
RACVKDGQVDQCAFDQLRKEYPLRREWAAHGGPVA

Specific function: Catalyzes the oxidation of erythronate-4-phosphate to 3- hydroxy-2-oxo-4-phosphonooxybutanoate

COG id: COG0111

COG function: function code HE; Phosphoglycerate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. PdxB subfamily

Homologues:

Organism=Homo sapiens, GI23308577, Length=266, Percent_Identity=30.4511278195489, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI145580578, Length=252, Percent_Identity=27.7777777777778, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI4557499, Length=252, Percent_Identity=27.7777777777778, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI145580575, Length=252, Percent_Identity=27.7777777777778, Blast_Score=75, Evalue=9e-14,
Organism=Homo sapiens, GI61743967, Length=252, Percent_Identity=27.3809523809524, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI4557497, Length=252, Percent_Identity=27.3809523809524, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1788660, Length=360, Percent_Identity=41.9444444444444, Blast_Score=229, Evalue=2e-61,
Organism=Escherichia coli, GI87082289, Length=265, Percent_Identity=31.3207547169811, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1789279, Length=259, Percent_Identity=30.8880308880309, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI1787645, Length=199, Percent_Identity=24.1206030150754, Blast_Score=71, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17532191, Length=258, Percent_Identity=29.8449612403101, Blast_Score=108, Evalue=6e-24,
Organism=Saccharomyces cerevisiae, GI6322116, Length=338, Percent_Identity=25.7396449704142, Blast_Score=95, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6320925, Length=340, Percent_Identity=25.2941176470588, Blast_Score=93, Evalue=8e-20,
Organism=Saccharomyces cerevisiae, GI6324055, Length=233, Percent_Identity=27.8969957081545, Blast_Score=87, Evalue=3e-18,
Organism=Drosophila melanogaster, GI19921140, Length=291, Percent_Identity=26.1168384879725, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24646446, Length=254, Percent_Identity=27.5590551181102, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24646448, Length=254, Percent_Identity=27.5590551181102, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24646452, Length=254, Percent_Identity=27.5590551181102, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24646450, Length=254, Percent_Identity=27.5590551181102, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI62472511, Length=254, Percent_Identity=27.5590551181102, Blast_Score=83, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PDXB_ACIAD (Q6F943)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_047244.1
- HSSP:   P0A9T0
- ProteinModelPortal:   Q6F943
- SMR:   Q6F943
- STRING:   Q6F943
- GeneID:   2878298
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD2667
- NMPDR:   fig|62977.3.peg.2437
- eggNOG:   COG0111
- HOGENOM:   HBG289972
- OMA:   SAPGCNA
- PhylomeDB:   Q6F943
- ProtClustDB:   CLSK707643
- BioCyc:   ASP62977:ACIAD2667-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01825
- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR020921
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR10996:SF4

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C

EC number: =1.1.1.290

Molecular weight: Translated: 39464; Mature: 39464

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1; PS00670 D_2_HYDROXYACID_DH_2; PS00671 D_2_HYDROXYACID_DH_3

Important sites: ACT_SITE 206-206 ACT_SITE 234-234 ACT_SITE 251-251 BINDING 45-45 BINDING 66-66 BINDING 146-146 BINDING 229-229 BINDING 254-254 BINDING 255-255

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIVADENLAFTDYFFSEFGEIQHRAGRLLTAHDVSDAEALLVRSVTKVNQALIEHSQLK
CEEECCCCCCHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHE
FVGSATIGTDHLDISALQQQDILWSNAPGCNAQAVAEYVITALYHLDSDVFERGQDFTLG
EEECCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHCCCCCEEE
IIGLGNVGRRLAKMAALLGWNVIGCDPFVQLPDIHNLSFDDVLQKSDAISVHVPLTHSGS
EEECCHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCHHHHHHCCCCEEEEEEEECCCC
HPTFHLFDQHAFASMPASTILINSARGPVIEEQALIQDIYQTGRKVVLDVFEHEPVISEQ
CCEEEEEHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHH
LLDVVNLVTPHIAGYSLEGKARGTQMIYDAFCKVFGYEASKKFETQLPVCEPFFQQQDLK
HHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHCCCCCCCHHHHHHHHH
QILKAHLRAIYDIAQDDHNLRACVKDGQVDQCAFDQLRKEYPLRREWAAHGGPVA
HHHHHHHHHHHHHHCCCCHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHCCCCCCC
>Mature Secondary Structure
MKIVADENLAFTDYFFSEFGEIQHRAGRLLTAHDVSDAEALLVRSVTKVNQALIEHSQLK
CEEECCCCCCHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHE
FVGSATIGTDHLDISALQQQDILWSNAPGCNAQAVAEYVITALYHLDSDVFERGQDFTLG
EEECCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHCCCCCEEE
IIGLGNVGRRLAKMAALLGWNVIGCDPFVQLPDIHNLSFDDVLQKSDAISVHVPLTHSGS
EEECCHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCHHHHHHCCCCEEEEEEEECCCC
HPTFHLFDQHAFASMPASTILINSARGPVIEEQALIQDIYQTGRKVVLDVFEHEPVISEQ
CCEEEEEHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHH
LLDVVNLVTPHIAGYSLEGKARGTQMIYDAFCKVFGYEASKKFETQLPVCEPFFQQQDLK
HHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHCCCCCCCHHHHHHHHH
QILKAHLRAIYDIAQDDHNLRACVKDGQVDQCAFDQLRKEYPLRREWAAHGGPVA
HHHHHHHHHHHHHHCCCCHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA