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The map label for this gene is yjeA [H]

Identifier: 50085733

GI number: 50085733

Start: 2620294

End: 2621283

Strand: Reverse

Name: yjeA [H]

Synonym: ACIAD2666

Alternate gene names: 50085733

Gene position: 2621283-2620294 (Counterclockwise)

Preceding gene: 50085734

Following gene: 50085730

Centisome position: 72.84

GC content: 42.02

Gene sequence:

>990_bases
GTGAATTTAGAGCATCACTCAAATCATTATCAACCTACGTGTTCTATCGAGGCACTTCAAGCCCGTGCCAAGCTTTACCA
AACTATTCGCACATTTTTTGCAGAGCGCGGCGTACTTGAAGTTGAAACACCTATTATTTCTCAGGCAGGGGTGACAGATG
TACATTTGGCTTCGGTTAAGGCATTACGCCATATTGAAGGTAAAGAACGTACTCAATATCTGCAAACTTCACCTGAATTT
GCGATGAAGCGTCTACTCGCCAGTGGTAGTGGGCCAATTTATCAGCTCTGTAAAGTATTTCGTGATGATGAACATGGACG
AAAACATAATAGTGAATTTACCATGCTGGAATGGTATCGCCCAGAATTCGATTTGAAAGATTTAATGCATGAAACGGCTG
ATTTACTTGAAACGTGTTTAAAGCAACGTTTTGATGAAATTCGCCCCATGATTTTGAGTTATAAACATGCCTTTCAGGAT
CGTTTAGAGATTAATCCATTACAAGCAACGTTAAAGCAGCTTAAAGATACGGCTGATCGTGTAGGTTTAAATCTCGACTT
GGGTAATGATCGCTTGGCCTATATAGATTTACTATTTTCCCATATTGTTGAACCGAGTCTAGGCTTCGATACGCCTGTAT
TTCTGACCGACTTTCCACCAGAAATGGCATCGCTTGCCAAAGTACGTATAGATGAAGATGGAGAAGAAGTTGCCGCACGT
TTTGAGGTATATATCGAAGGACTGGAGCTTGCCAATGCTTATGACGAGCTTCTTGATGCAAATATACTCAGAGCACGCTT
TGAAGCAGATAATTTAGAACGCAAATTGCAAGGTTTGGCAGAAATGCCACTCGATGAAAACTTGCTGGCTGCTTTGCCTC
ATATGCCAGCCTGTGCGGGTATCGCACTTGGCGTAGATCGGTTACTGATGATTGCAACTCGACAGCTTAAAATTGAACAG
GTGATTGCATTTCCTGCTGCAATTGCTTGA

Upstream 100 bases:

>100_bases
CTTGTGTAAAAGATGGACAAGTTGATCAGTGTGCTTTTGATCAGCTTCGAAAGGAATACCCGCTTCGCCGTGAATGGGCA
GCGCATGGAGGCCCTGTCGC

Downstream 100 bases:

>100_bases
TAAAAAATAAGCCGCTGATCGCGGCTTACTTTTTATGATGTCTTTAATTAATCCTGCTGAATACCAGCCACTTTCCACTC
TTCAGTTGAACCTGCTGGTT

Product: lysyl-tRNA synthetase (lysine--tRNA ligase)(PoxA)

Products: AMP; diphosphate; L-lysyl-tRNA(Lys)

Alternate protein names: NA

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MNLEHHSNHYQPTCSIEALQARAKLYQTIRTFFAERGVLEVETPIISQAGVTDVHLASVKALRHIEGKERTQYLQTSPEF
AMKRLLASGSGPIYQLCKVFRDDEHGRKHNSEFTMLEWYRPEFDLKDLMHETADLLETCLKQRFDEIRPMILSYKHAFQD
RLEINPLQATLKQLKDTADRVGLNLDLGNDRLAYIDLLFSHIVEPSLGFDTPVFLTDFPPEMASLAKVRIDEDGEEVAAR
FEVYIEGLELANAYDELLDANILRARFEADNLERKLQGLAEMPLDENLLAALPHMPACAGIALGVDRLLMIATRQLKIEQ
VIAFPAAIA

Sequences:

>Translated_329_residues
MNLEHHSNHYQPTCSIEALQARAKLYQTIRTFFAERGVLEVETPIISQAGVTDVHLASVKALRHIEGKERTQYLQTSPEF
AMKRLLASGSGPIYQLCKVFRDDEHGRKHNSEFTMLEWYRPEFDLKDLMHETADLLETCLKQRFDEIRPMILSYKHAFQD
RLEINPLQATLKQLKDTADRVGLNLDLGNDRLAYIDLLFSHIVEPSLGFDTPVFLTDFPPEMASLAKVRIDEDGEEVAAR
FEVYIEGLELANAYDELLDANILRARFEADNLERKLQGLAEMPLDENLLAALPHMPACAGIALGVDRLLMIATRQLKIEQ
VIAFPAAIA
>Mature_329_residues
MNLEHHSNHYQPTCSIEALQARAKLYQTIRTFFAERGVLEVETPIISQAGVTDVHLASVKALRHIEGKERTQYLQTSPEF
AMKRLLASGSGPIYQLCKVFRDDEHGRKHNSEFTMLEWYRPEFDLKDLMHETADLLETCLKQRFDEIRPMILSYKHAFQD
RLEINPLQATLKQLKDTADRVGLNLDLGNDRLAYIDLLFSHIVEPSLGFDTPVFLTDFPPEMASLAKVRIDEDGEEVAAR
FEVYIEGLELANAYDELLDANILRARFEADNLERKLQGLAEMPLDENLLAALPHMPACAGIALGVDRLLMIATRQLKIEQ
VIAFPAAIA

Specific function: Could Be A Lysyl-Trna Synthetase. Mutants In Poxa Have A Reduced Pyruvate Oxidase Activity And A Reduced Growth Rate. [C]

COG id: COG2269

COG function: function code J; Truncated, possibly inactive, lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI5031815, Length=341, Percent_Identity=26.6862170087977, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI194272210, Length=341, Percent_Identity=26.6862170087977, Blast_Score=98, Evalue=1e-20,
Organism=Escherichia coli, GI87082379, Length=322, Percent_Identity=47.5155279503106, Blast_Score=276, Evalue=2e-75,
Organism=Escherichia coli, GI1789256, Length=329, Percent_Identity=28.8753799392097, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1790571, Length=331, Percent_Identity=29.3051359516616, Blast_Score=124, Evalue=8e-30,
Organism=Saccharomyces cerevisiae, GI6324256, Length=352, Percent_Identity=27.8409090909091, Blast_Score=99, Evalue=6e-22,
Organism=Saccharomyces cerevisiae, GI6320242, Length=341, Percent_Identity=27.2727272727273, Blast_Score=99, Evalue=8e-22,
Organism=Drosophila melanogaster, GI24640851, Length=340, Percent_Identity=25.5882352941176, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24640849, Length=349, Percent_Identity=26.3610315186246, Blast_Score=96, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR004525
- InterPro:   IPR018149 [H]

Pfam domain/function: PF00152 tRNA-synt_2 [H]

EC number: 6.1.1.6

Molecular weight: Translated: 37374; Mature: 37374

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLEHHSNHYQPTCSIEALQARAKLYQTIRTFFAERGVLEVETPIISQAGVTDVHLASVK
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCHHHHHHHH
ALRHIEGKERTQYLQTSPEFAMKRLLASGSGPIYQLCKVFRDDEHGRKHNSEFTMLEWYR
HHHHCCCHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCEEEEECC
PEFDLKDLMHETADLLETCLKQRFDEIRPMILSYKHAFQDRLEINPLQATLKQLKDTADR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
VGLNLDLGNDRLAYIDLLFSHIVEPSLGFDTPVFLTDFPPEMASLAKVRIDEDGEEVAAR
CCCEEECCCCHHHHHHHHHHHHCCCCCCCCCCEEEECCCHHHHHHHHHHCCCCHHHHHHH
FEVYIEGLELANAYDELLDANILRARFEADNLERKLQGLAEMPLDENLLAALPHMPACAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCHHHH
IALGVDRLLMIATRQLKIEQVIAFPAAIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNLEHHSNHYQPTCSIEALQARAKLYQTIRTFFAERGVLEVETPIISQAGVTDVHLASVK
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCHHHHHHHH
ALRHIEGKERTQYLQTSPEFAMKRLLASGSGPIYQLCKVFRDDEHGRKHNSEFTMLEWYR
HHHHCCCHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCEEEEECC
PEFDLKDLMHETADLLETCLKQRFDEIRPMILSYKHAFQDRLEINPLQATLKQLKDTADR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
VGLNLDLGNDRLAYIDLLFSHIVEPSLGFDTPVFLTDFPPEMASLAKVRIDEDGEEVAAR
CCCEEECCCCHHHHHHHHHHHHCCCCCCCCCCEEEECCCHHHHHHHHHHCCCCHHHHHHH
FEVYIEGLELANAYDELLDANILRARFEADNLERKLQGLAEMPLDENLLAALPHMPACAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCHHHH
IALGVDRLLMIATRQLKIEQVIAFPAAIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 2454 [C]

Specific activity: 1.79

Km value (mM): 0.0236 {L-Lys}} 0.0164 {L-Lys}} 0.0651 {ATP}} 0.0232 {ATP}} [C]

Substrates: ATP; L-lysine; tRNA(Lys)

Specific reaction: ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys)

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 6-Amino-n-hexanoic acid; Adenosine; ATP; Cadaverine; L-Lysineamide; L-Lysinehydroxamate; N-epsilon-Acetyl -L-lysine [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA