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The map label for this gene is dapF

Identifier: 50085727

GI number: 50085727

Start: 2613423

End: 2614268

Strand: Reverse

Name: dapF

Synonym: ACIAD2659

Alternate gene names: 50085727

Gene position: 2614268-2613423 (Counterclockwise)

Preceding gene: 50085728

Following gene: 50085726

Centisome position: 72.65

GC content: 44.92

Gene sequence:

>846_bases
ATGTTACTTGAGTTTACCAAAATGCATGGGTTGGGTAACGACTTCGTTGTCCTTGATCTCATCAGTCAGCGTGCCTATTT
AGATACCACCACCATCCAGCGTATGGCCGATCGTCACTTCGGTATTGGTTTTGATCAGTTACTCATTGTTGAGCCACCTG
ATTATCCAAATGTTGATTTCAAATATCGTATTTTTAATGCAGATGGTTCAGAGGTAGAGCAGTGTGGAAATGGTGTACGC
TGTTTTGCACGTTTTGTACATGAGCGCCAACTCACCAAGAAAACCAAAATCAAGGTACAAACTAAAGCGGGTATTGTCGA
GCCAGAGCTAGGGCCAAACGGATGGGTTCGTGTAAATATGGGCTATCCGAAATTTATGCCACATGAAATTCCGTTTGTAA
CCGAAGAATTTGAAGCACTGTATACGCTTGAACTTGCCAATGAGCAAAGTTTAAAAATTGATGTGGTTAATATGGGTAAT
CCACATGCAGTGACCATTGTGCCAGATGTTTTAACAGCAGATGTTGCAACGATGGGGCCACAAGTAGAGTCTCATGTCCG
TTTTCCTCAGCGCGTTAATGCTGGCTTTATGCAGATTGTGGATGAAAAGCACATACGCCTACGAGTGTTTGAACGTGGTG
TGGGTGAAACACTTGCCTGTGGTACAGGTGCATGTGCTGCTGCCGTGAGTGGTATGCGACGTGGTTTACTTGCCAATGAG
GTGGAAGTGGAACTGGCTGGTGGAAAACTGCAAATTGCCTGGCAAGAAGGTGATGTGGTCTGGATGACGGGTCCAACAGC
AAATGTTTATGAAGGGCGTCTGGATTTACGTTACTTCCAAAGCTGA

Upstream 100 bases:

>100_bases
TGAGGTCATGGTCGATGATGAAAAACCTCATCTGATTCGTGAGCGTGAAAGCATTGAATCGTTGTGGGAAAGAGAGCGTT
TGTTGCCTGAGGAGTAAGAC

Downstream 100 bases:

>100_bases
TTGTAGATTAAATCTATACATTAAAAGCACTATTTTTAGGATAGTGCTTTTTTTATGGGGCATTTCAGCGTAAGGTGATT
TTTCAATATCAATACAATGG

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MLLEFTKMHGLGNDFVVLDLISQRAYLDTTTIQRMADRHFGIGFDQLLIVEPPDYPNVDFKYRIFNADGSEVEQCGNGVR
CFARFVHERQLTKKTKIKVQTKAGIVEPELGPNGWVRVNMGYPKFMPHEIPFVTEEFEALYTLELANEQSLKIDVVNMGN
PHAVTIVPDVLTADVATMGPQVESHVRFPQRVNAGFMQIVDEKHIRLRVFERGVGETLACGTGACAAAVSGMRRGLLANE
VEVELAGGKLQIAWQEGDVVWMTGPTANVYEGRLDLRYFQS

Sequences:

>Translated_281_residues
MLLEFTKMHGLGNDFVVLDLISQRAYLDTTTIQRMADRHFGIGFDQLLIVEPPDYPNVDFKYRIFNADGSEVEQCGNGVR
CFARFVHERQLTKKTKIKVQTKAGIVEPELGPNGWVRVNMGYPKFMPHEIPFVTEEFEALYTLELANEQSLKIDVVNMGN
PHAVTIVPDVLTADVATMGPQVESHVRFPQRVNAGFMQIVDEKHIRLRVFERGVGETLACGTGACAAAVSGMRRGLLANE
VEVELAGGKLQIAWQEGDVVWMTGPTANVYEGRLDLRYFQS
>Mature_281_residues
MLLEFTKMHGLGNDFVVLDLISQRAYLDTTTIQRMADRHFGIGFDQLLIVEPPDYPNVDFKYRIFNADGSEVEQCGNGVR
CFARFVHERQLTKKTKIKVQTKAGIVEPELGPNGWVRVNMGYPKFMPHEIPFVTEEFEALYTLELANEQSLKIDVVNMGN
PHAVTIVPDVLTADVATMGPQVESHVRFPQRVNAGFMQIVDEKHIRLRVFERGVGETLACGTGACAAAVSGMRRGLLANE
VEVELAGGKLQIAWQEGDVVWMTGPTANVYEGRLDLRYFQS

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family

Homologues:

Organism=Escherichia coli, GI87082334, Length=275, Percent_Identity=56, Blast_Score=315, Evalue=3e-87,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPF_ACIAD (Q6F950)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_047237.1
- HSSP:   P44859
- ProteinModelPortal:   Q6F950
- SMR:   Q6F950
- STRING:   Q6F950
- GeneID:   2878257
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD2659
- NMPDR:   fig|62977.3.peg.2430
- eggNOG:   COG0253
- HOGENOM:   HBG399442
- OMA:   HTGIGFD
- PhylomeDB:   Q6F950
- ProtClustDB:   PRK00450
- BioCyc:   ASP62977:ACIAD2659-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00197
- InterPro:   IPR001653
- InterPro:   IPR018510
- TIGRFAMs:   TIGR00652

Pfam domain/function: PF01678 DAP_epimerase

EC number: =5.1.1.7

Molecular weight: Translated: 31462; Mature: 31462

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: ACT_SITE 75-75 ACT_SITE 220-220

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLEFTKMHGLGNDFVVLDLISQRAYLDTTTIQRMADRHFGIGFDQLLIVEPPDYPNVDF
CEEEEEHHCCCCCCEEEEEEHHCCHHCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCE
KYRIFNADGSEVEQCGNGVRCFARFVHERQLTKKTKIKVQTKAGIVEPELGPNGWVRVNM
EEEEECCCCHHHHHHCCHHHHHHHHHHHHHHCHHHEEEEEECCCEECCCCCCCCEEEEEC
GYPKFMPHEIPFVTEEFEALYTLELANEQSLKIDVVNMGNPHAVTIVPDVLTADVATMGP
CCCCCCCCCCCCHHHCCCEEEEEEECCCCCEEEEEEECCCCEEEEEECCHHHHHHHHCCC
QVESHVRFPQRVNAGFMQIVDEKHIRLRVFERGVGETLACGTGACAAAVSGMRRGLLANE
CHHHHCCCCHHCCCHHHHHHCCCCEEEEEEECCCCCEECCCCCHHHHHHHHHHCCCCCCE
VEVELAGGKLQIAWQEGDVVWMTGPTANVYEGRLDLRYFQS
EEEEEECCEEEEEEECCCEEEEECCCCCEECCEEEEEEECC
>Mature Secondary Structure
MLLEFTKMHGLGNDFVVLDLISQRAYLDTTTIQRMADRHFGIGFDQLLIVEPPDYPNVDF
CEEEEEHHCCCCCCEEEEEEHHCCHHCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCE
KYRIFNADGSEVEQCGNGVRCFARFVHERQLTKKTKIKVQTKAGIVEPELGPNGWVRVNM
EEEEECCCCHHHHHHCCHHHHHHHHHHHHHHCHHHEEEEEECCCEECCCCCCCCEEEEEC
GYPKFMPHEIPFVTEEFEALYTLELANEQSLKIDVVNMGNPHAVTIVPDVLTADVATMGP
CCCCCCCCCCCCHHHCCCEEEEEEECCCCCEEEEEEECCCCEEEEEECCHHHHHHHHCCC
QVESHVRFPQRVNAGFMQIVDEKHIRLRVFERGVGETLACGTGACAAAVSGMRRGLLANE
CHHHHCCCCHHCCCHHHHHHCCCCEEEEEEECCCCCEECCCCCHHHHHHHHHHCCCCCCE
VEVELAGGKLQIAWQEGDVVWMTGPTANVYEGRLDLRYFQS
EEEEEECCEEEEEEECCCEEEEECCCCCEECCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA