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The map label for this gene is dusB [H]

Identifier: 50085634

GI number: 50085634

Start: 2516779

End: 2517804

Strand: Reverse

Name: dusB [H]

Synonym: ACIAD2557

Alternate gene names: 50085634

Gene position: 2517804-2516779 (Counterclockwise)

Preceding gene: 50085637

Following gene: 50085632

Centisome position: 69.97

GC content: 43.27

Gene sequence:

>1026_bases
ATGTATATTGGGTCTTATCAACTGTCAAATAATTTAATTGTAGCTCCTATGGCGGGAGTGACAGATCGCCCGTTCCGTAC
ACTGTGTAAATATTTTGGTGCAGGGCATGCGGTAAGTGAAATGATGACCGCAGATAAGACTTTAAGAATGAGTAAAAAAA
GCCTGTATCGGGCCAATTTTGATGGAGAACTCGCACCGATTTCAGCACAAATTGCAGGTTCTGACCCAGAGCAACTCGCT
GAAGCTGCACGCTATCAGGTCGCAAATGGCGCCCAGATTGTGGATATCAACATGGGCTGTCCTGCCAAAAAAGTCTGTAA
CAAGCTTGCTGGTTCTGCACTTTTACAAGATGAAGACTTGGTTGCTCGTATTCTGGATGCAGTAGTTGCAGCGGTGGATG
TTCCTGTGACGCTAAAAACCCGTTTAGGTTTCTTAAATGGGCAGGAAAATATTCTTAGAGTTGCCAAGCAAGCTGAGCAG
TCTGGTATTGCTGCACTGGCACTCCATGGTCGTACACGTGAAGACATGTATTTAAGTACTGCGCGTTATGATTTGATCAA
ACAGGTGAAATCGATTTTAACTATTCCTGTGATTGCAAATGGGGATATCGACAGTCCGCAAAAAGCCAAATATGTTTTGG
ATTATACAGGTGCTGACGCCATTATGATTGGTCGTGCTGCGCAAGGACGCCCTTGGATTTTTCGTGAAATTGCACATTAT
TTGAAAACAGGCGAGCATCTGGCTGCACCTGATATTGCAGAAGTGAAAGAGGTTTTACTGGGACATTTGTCCGAGTTATA
TAGGTTTTATGGTGAATATTCAGGATGTCGAATCGCTCGAAAACACATTGCCTGGTACACCAAAGGTCTGCATTCAAGCA
ATGAGTTTCGTCAAAACATGTATCAAGTGGAAAGTACGGCTGAACAAGCTCGCCTAGTTGAAGCTTATTTCAATCATTTA
TTGATGCAAGGCCAGTTAATGAGCGATGTTCAGACCGAACACGTCGATTTGTTGTCGATTAAATAA

Upstream 100 bases:

>100_bases
AATAGATGAATTATTCATGCAAGCAAAACACCTTGATAAAAAGATGAGTCAAAAAGGCGGATGAAGTATACTATGTCGAT
CTTAAAATGTATGAATATAT

Downstream 100 bases:

>100_bases
TTTAGGCACTCATTTCCAAAAGCAATTGATTAATGAGATCAGTTGCCTCTAGCTCGCGAATTTGTGCTACATGACTTCCA
GCCCAAAAAGCACCAAAGCC

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MYIGSYQLSNNLIVAPMAGVTDRPFRTLCKYFGAGHAVSEMMTADKTLRMSKKSLYRANFDGELAPISAQIAGSDPEQLA
EAARYQVANGAQIVDINMGCPAKKVCNKLAGSALLQDEDLVARILDAVVAAVDVPVTLKTRLGFLNGQENILRVAKQAEQ
SGIAALALHGRTREDMYLSTARYDLIKQVKSILTIPVIANGDIDSPQKAKYVLDYTGADAIMIGRAAQGRPWIFREIAHY
LKTGEHLAAPDIAEVKEVLLGHLSELYRFYGEYSGCRIARKHIAWYTKGLHSSNEFRQNMYQVESTAEQARLVEAYFNHL
LMQGQLMSDVQTEHVDLLSIK

Sequences:

>Translated_341_residues
MYIGSYQLSNNLIVAPMAGVTDRPFRTLCKYFGAGHAVSEMMTADKTLRMSKKSLYRANFDGELAPISAQIAGSDPEQLA
EAARYQVANGAQIVDINMGCPAKKVCNKLAGSALLQDEDLVARILDAVVAAVDVPVTLKTRLGFLNGQENILRVAKQAEQ
SGIAALALHGRTREDMYLSTARYDLIKQVKSILTIPVIANGDIDSPQKAKYVLDYTGADAIMIGRAAQGRPWIFREIAHY
LKTGEHLAAPDIAEVKEVLLGHLSELYRFYGEYSGCRIARKHIAWYTKGLHSSNEFRQNMYQVESTAEQARLVEAYFNHL
LMQGQLMSDVQTEHVDLLSIK
>Mature_341_residues
MYIGSYQLSNNLIVAPMAGVTDRPFRTLCKYFGAGHAVSEMMTADKTLRMSKKSLYRANFDGELAPISAQIAGSDPEQLA
EAARYQVANGAQIVDINMGCPAKKVCNKLAGSALLQDEDLVARILDAVVAAVDVPVTLKTRLGFLNGQENILRVAKQAEQ
SGIAALALHGRTREDMYLSTARYDLIKQVKSILTIPVIANGDIDSPQKAKYVLDYTGADAIMIGRAAQGRPWIFREIAHY
LKTGEHLAAPDIAEVKEVLLGHLSELYRFYGEYSGCRIARKHIAWYTKGLHSSNEFRQNMYQVESTAEQARLVEAYFNHL
LMQGQLMSDVQTEHVDLLSIK

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusB subfamily [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=234, Percent_Identity=31.1965811965812, Blast_Score=112, Evalue=7e-25,
Organism=Homo sapiens, GI8923374, Length=255, Percent_Identity=33.7254901960784, Blast_Score=111, Evalue=8e-25,
Organism=Homo sapiens, GI239788462, Length=235, Percent_Identity=31.063829787234, Blast_Score=108, Evalue=5e-24,
Organism=Homo sapiens, GI31742496, Length=225, Percent_Identity=30.2222222222222, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI40807366, Length=228, Percent_Identity=30.2631578947368, Blast_Score=90, Evalue=2e-18,
Organism=Escherichia coli, GI1789660, Length=320, Percent_Identity=56.5625, Blast_Score=355, Evalue=2e-99,
Organism=Escherichia coli, GI1788462, Length=316, Percent_Identity=28.4810126582279, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI145693211, Length=252, Percent_Identity=31.3492063492063, Blast_Score=86, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17510279, Length=245, Percent_Identity=31.8367346938775, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17543114, Length=242, Percent_Identity=29.3388429752066, Blast_Score=87, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI25144369, Length=216, Percent_Identity=30.0925925925926, Blast_Score=87, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI17507177, Length=241, Percent_Identity=27.8008298755187, Blast_Score=78, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6323560, Length=232, Percent_Identity=31.8965517241379, Blast_Score=96, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6323433, Length=310, Percent_Identity=26.1290322580645, Blast_Score=91, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6323437, Length=225, Percent_Identity=25.3333333333333, Blast_Score=86, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24585320, Length=234, Percent_Identity=31.6239316239316, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24580595, Length=273, Percent_Identity=30.4029304029304, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI19920448, Length=273, Percent_Identity=30.4029304029304, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI19921524, Length=230, Percent_Identity=30, Blast_Score=95, Evalue=8e-20,
Organism=Drosophila melanogaster, GI45549423, Length=245, Percent_Identity=31.0204081632653, Blast_Score=93, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 37714; Mature: 37714

Theoretical pI: Translated: 7.63; Mature: 7.63

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYIGSYQLSNNLIVAPMAGVTDRPFRTLCKYFGAGHAVSEMMTADKTLRMSKKSLYRANF
CCCCCEEECCCEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCC
DGELAPISAQIAGSDPEQLAEAARYQVANGAQIVDINMGCPAKKVCNKLAGSALLQDEDL
CCCCCCEEEEECCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHCCCHHH
VARILDAVVAAVDVPVTLKTRLGFLNGQENILRVAKQAEQSGIAALALHGRTREDMYLST
HHHHHHHHHHHHCCCEEHHHHHCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHH
ARYDLIKQVKSILTIPVIANGDIDSPQKAKYVLDYTGADAIMIGRAAQGRPWIFREIAHY
HHHHHHHHHHHHHEEEEEECCCCCCCCCCEEEEEECCCCEEEEECCCCCCCHHHHHHHHH
LKTGEHLAAPDIAEVKEVLLGHLSELYRFYGEYSGCRIARKHIAWYTKGLHSSNEFRQNM
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHH
YQVESTAEQARLVEAYFNHLLMQGQLMSDVQTEHVDLLSIK
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCEEEEECC
>Mature Secondary Structure
MYIGSYQLSNNLIVAPMAGVTDRPFRTLCKYFGAGHAVSEMMTADKTLRMSKKSLYRANF
CCCCCEEECCCEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCC
DGELAPISAQIAGSDPEQLAEAARYQVANGAQIVDINMGCPAKKVCNKLAGSALLQDEDL
CCCCCCEEEEECCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHCCCHHH
VARILDAVVAAVDVPVTLKTRLGFLNGQENILRVAKQAEQSGIAALALHGRTREDMYLST
HHHHHHHHHHHHCCCEEHHHHHCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHH
ARYDLIKQVKSILTIPVIANGDIDSPQKAKYVLDYTGADAIMIGRAAQGRPWIFREIAHY
HHHHHHHHHHHHHEEEEEECCCCCCCCCCEEEEEECCCCEEEEECCCCCCCHHHHHHHHH
LKTGEHLAAPDIAEVKEVLLGHLSELYRFYGEYSGCRIARKHIAWYTKGLHSSNEFRQNM
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHH
YQVESTAEQARLVEAYFNHLLMQGQLMSDVQTEHVDLLSIK
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]