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The map label for this gene is proB

Identifier: 50085637

GI number: 50085637

Start: 2519746

End: 2520879

Strand: Reverse

Name: proB

Synonym: ACIAD2560

Alternate gene names: 50085637

Gene position: 2520879-2519746 (Counterclockwise)

Preceding gene: 50085638

Following gene: 50085634

Centisome position: 70.05

GC content: 45.86

Gene sequence:

>1134_bases
ATGATAGAAGTGATCGATGGGCAACGACAGCTCAAGGCGTGCAAACGTATCGTTGTTAAAATCGGATCATCTCTACTGAC
AGCCAATGGACAAGGTTTAGATCTGGATGCGATTTCGCATTGGGCAATGCAAATTGCTGATCTACACAATGCTGGACACG
AAATTATTTTGGTGTCTTCAGGGGCTGTAGCAGAAGGGATGGTGCGTATGAAACTAACGAGTCGACCCACCGATCTACCC
AGTTTGCAAGCCTGTGCGGCGATTGGTCAAATGGGCCTGATTCATACGTGGTCGAGTGTCCTTGAAAAGCATACGATTCA
GACTGCTCAAGTACTGCTCACACATGATGATCTGGCAGATCGTCGTCGTTATTTAAACTCGTGTGATGCCTTACAAAACT
TGATTGAGTGGCGAGTGATTCCTGTTATCAACGAAAATGATACAGTATCTACTGATGAAATTCGTTTTGGTGATAATGAT
ACACTCGCTGCGATGGTGGCAGGTCAGGTTCATGCTGATTTGCTGATCATTCTGACTGATCAGCAAGGGATGTTTGATTC
AGATCCACGTAGTAATCCGAATGCAAAATTACTCACTACTGTTAATGCGATGGATGATGCGTTGTTTGAAATGGCAGGTG
GCGGCGGTGTACTCGGACGTGGTGGTATGGTCACCAAGGTTCGTGCTGCACGTCTGGCGGCAAAATCTGGTTGTCCAACC
TTGATTGCAAGTGGTGAAAGTGATCATGTTTTATCACGCCTGATGTCAGGTGAGTTACTTGGTACTTTATTTAGCACTGA
TAAAGATCGGATTACAGCGCATCAGCAATGGCTGGCTGCCCATCTTCAAACTGCGGGTCGTCTGGTGATTGATGCAGGTG
CAGTTGAAGCGATTAAGCAACGACATCGTAGTTTATTGCCAGTTGGGGTGAAGGCTGTTGAAGGTCATTTTAACCGTGGC
GATGTGGTCGAATGTGTTGATCAGTCTGGGCAGCGTGTTGCAGTCGGGCGCGTGAATTTTAGTTCACGTTCGGCTGAGAT
CATTAAAGGTTTGGCTTCAGATAAAGTACATCAAGTGTTAGGTGAAGCACGTTCTCTGGAAATGATTCATCGCAATCATA
TGGCGATTTATTAA

Upstream 100 bases:

>100_bases
GCGTAAAGCACAGCGTGAAGCAGATCAGGATGATGATTTTGACGATGATGATGACGAAGTGGAAGTAATTTACGTTCGTT
AAGTAGAACTCGAGGAAAAA

Downstream 100 bases:

>100_bases
TTGGATTAAGTCCTGTAAAAAGCTCGCTTAGGCGAGCTTTTTTACAGCTTGAAGTAAAGGTAAGCTTTGATCTTCACCGA
CCATATACGACGTATCAGCC

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK

Number of amino acids: Translated: 377; Mature: 377

Protein sequence:

>377_residues
MIEVIDGQRQLKACKRIVVKIGSSLLTANGQGLDLDAISHWAMQIADLHNAGHEIILVSSGAVAEGMVRMKLTSRPTDLP
SLQACAAIGQMGLIHTWSSVLEKHTIQTAQVLLTHDDLADRRRYLNSCDALQNLIEWRVIPVINENDTVSTDEIRFGDND
TLAAMVAGQVHADLLIILTDQQGMFDSDPRSNPNAKLLTTVNAMDDALFEMAGGGGVLGRGGMVTKVRAARLAAKSGCPT
LIASGESDHVLSRLMSGELLGTLFSTDKDRITAHQQWLAAHLQTAGRLVIDAGAVEAIKQRHRSLLPVGVKAVEGHFNRG
DVVECVDQSGQRVAVGRVNFSSRSAEIIKGLASDKVHQVLGEARSLEMIHRNHMAIY

Sequences:

>Translated_377_residues
MIEVIDGQRQLKACKRIVVKIGSSLLTANGQGLDLDAISHWAMQIADLHNAGHEIILVSSGAVAEGMVRMKLTSRPTDLP
SLQACAAIGQMGLIHTWSSVLEKHTIQTAQVLLTHDDLADRRRYLNSCDALQNLIEWRVIPVINENDTVSTDEIRFGDND
TLAAMVAGQVHADLLIILTDQQGMFDSDPRSNPNAKLLTTVNAMDDALFEMAGGGGVLGRGGMVTKVRAARLAAKSGCPT
LIASGESDHVLSRLMSGELLGTLFSTDKDRITAHQQWLAAHLQTAGRLVIDAGAVEAIKQRHRSLLPVGVKAVEGHFNRG
DVVECVDQSGQRVAVGRVNFSSRSAEIIKGLASDKVHQVLGEARSLEMIHRNHMAIY
>Mature_377_residues
MIEVIDGQRQLKACKRIVVKIGSSLLTANGQGLDLDAISHWAMQIADLHNAGHEIILVSSGAVAEGMVRMKLTSRPTDLP
SLQACAAIGQMGLIHTWSSVLEKHTIQTAQVLLTHDDLADRRRYLNSCDALQNLIEWRVIPVINENDTVSTDEIRFGDND
TLAAMVAGQVHADLLIILTDQQGMFDSDPRSNPNAKLLTTVNAMDDALFEMAGGGGVLGRGGMVTKVRAARLAAKSGCPT
LIASGESDHVLSRLMSGELLGTLFSTDKDRITAHQQWLAAHLQTAGRLVIDAGAVEAIKQRHRSLLPVGVKAVEGHFNRG
DVVECVDQSGQRVAVGRVNFSSRSAEIIKGLASDKVHQVLGEARSLEMIHRNHMAIY

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain

Homologues:

Organism=Homo sapiens, GI62912457, Length=292, Percent_Identity=34.9315068493151, Blast_Score=142, Evalue=6e-34,
Organism=Homo sapiens, GI21361368, Length=294, Percent_Identity=34.6938775510204, Blast_Score=142, Evalue=6e-34,
Organism=Escherichia coli, GI1786437, Length=359, Percent_Identity=39.8328690807799, Blast_Score=258, Evalue=4e-70,
Organism=Caenorhabditis elegans, GI17569967, Length=300, Percent_Identity=31, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17569969, Length=300, Percent_Identity=31, Blast_Score=139, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6320506, Length=417, Percent_Identity=33.3333333333333, Blast_Score=201, Evalue=1e-52,
Organism=Saccharomyces cerevisiae, GI6321822, Length=416, Percent_Identity=31.4903846153846, Blast_Score=191, Evalue=1e-49,
Organism=Drosophila melanogaster, GI21357643, Length=288, Percent_Identity=32.9861111111111, Blast_Score=141, Evalue=8e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROB_ACIAD (Q6F9D9)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_047147.1
- ProteinModelPortal:   Q6F9D9
- SMR:   Q6F9D9
- STRING:   Q6F9D9
- GeneID:   2880046
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD2560
- NMPDR:   fig|62977.3.peg.2340
- eggNOG:   COG0263
- HOGENOM:   HBG507643
- OMA:   TFGDNDM
- PhylomeDB:   Q6F9D9
- ProtClustDB:   PRK05429
- BioCyc:   ASP62977:ACIAD2560-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00456
- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947
- Gene3D:   G3DSA:3.40.1160.10
- PIRSF:   PIRSF000729
- PRINTS:   PR00474
- SMART:   SM00359
- TIGRFAMs:   TIGR01027

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA; SSF53633 Aa_kinase; SSF88697 PUA-like

EC number: =2.7.2.11

Molecular weight: Translated: 40717; Mature: 40717

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00902 GLUTAMATE_5_KINASE; PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEVIDGQRQLKACKRIVVKIGSSLLTANGQGLDLDAISHWAMQIADLHNAGHEIILVSS
CCEEECCHHHHHHHHHHHHHHCCHHEECCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEC
GAVAEGMVRMKLTSRPTDLPSLQACAAIGQMGLIHTWSSVLEKHTIQTAQVLLTHDDLAD
CCHHHCEEEEEECCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHEEEECCCHHH
RRRYLNSCDALQNLIEWRVIPVINENDTVSTDEIRFGDNDTLAAMVAGQVHADLLIILTD
HHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCEEECCCCCCHHHHHCCCCCEEEEEEEC
QQGMFDSDPRSNPNAKLLTTVNAMDDALFEMAGGGGVLGRGGMVTKVRAARLAAKSGCPT
CCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHCCCCCEECCCCCHHHHHHHHHHHCCCCCE
LIASGESDHVLSRLMSGELLGTLFSTDKDRITAHQQWLAAHLQTAGRLVIDAGAVEAIKQ
EEECCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCEEEEECHHHHHHHH
RHRSLLPVGVKAVEGHFNRGDVVECVDQSGQRVAVGRVNFSSRSAEIIKGLASDKVHQVL
HHHCCCCCCHHHHCCCCCCCCHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHH
GEARSLEMIHRNHMAIY
HHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MIEVIDGQRQLKACKRIVVKIGSSLLTANGQGLDLDAISHWAMQIADLHNAGHEIILVSS
CCEEECCHHHHHHHHHHHHHHCCHHEECCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEC
GAVAEGMVRMKLTSRPTDLPSLQACAAIGQMGLIHTWSSVLEKHTIQTAQVLLTHDDLAD
CCHHHCEEEEEECCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHEEEECCCHHH
RRRYLNSCDALQNLIEWRVIPVINENDTVSTDEIRFGDNDTLAAMVAGQVHADLLIILTD
HHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCEEECCCCCCHHHHHCCCCCEEEEEEEC
QQGMFDSDPRSNPNAKLLTTVNAMDDALFEMAGGGGVLGRGGMVTKVRAARLAAKSGCPT
CCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHCCCCCEECCCCCHHHHHHHHHHHCCCCCE
LIASGESDHVLSRLMSGELLGTLFSTDKDRITAHQQWLAAHLQTAGRLVIDAGAVEAIKQ
EEECCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCEEEEECHHHHHHHH
RHRSLLPVGVKAVEGHFNRGDVVECVDQSGQRVAVGRVNFSSRSAEIIKGLASDKVHQVL
HHHCCCCCCHHHHCCCCCCCCHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHH
GEARSLEMIHRNHMAIY
HHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA