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The map label for this gene is folD

Identifier: 50085630

GI number: 50085630

Start: 2513132

End: 2514025

Strand: Reverse

Name: folD

Synonym: ACIAD2553

Alternate gene names: 50085630

Gene position: 2514025-2513132 (Counterclockwise)

Preceding gene: 50085631

Following gene: 50085629

Centisome position: 69.86

GC content: 45.41

Gene sequence:

>894_bases
ATGAGTCTTGCCTTATTACAACAGCACAGTCCCAAAATTATTGATGGTAAAGCCATTGCTCAAAGCGTACTTGAGGAAGT
CAAGCTAGAAGTTGAGCGTTATAAAGCTCAAGGCATCGAGCCATGTCTGGCCGTTGTACTGGTTGGAGAAGACCCAGCAA
GTCAGGTGTATGTGCGTAATAAAGTTCAAAAAGCGGCATTTGTTGGGATTCGTTCACTCGAATTTCGCTTCGATGCTGAC
CTGAGTGAAGATGAACTGCTTACGCAAATTGATCGGCTAAATACAGAGCGCGGCGTACATGGCATTTTGGTGCAGTTACC
TTTACCCAAACAGATCAATGAAACCCGCGTACTTGAACGGATTCACCCTCAAAAAGATGTGGATGGTTTTCACAGTGAAA
ACGTGGGGGGACTAAGTCAAGGACGTGATGTTTTGACACCATGTACACCAACCGGCTGTATTCGTTTACTTAAGGACAGC
TTGGGTGATGATTTAAGTGGTTTACATGCGGTCGTGATTGGGCGCTCTAACATTGTAGGTAAACCGATGGCGGCATTACT
GCTTAAAGAGAGCTGTACAGTTACAGTGGTTCATTCCAAGACCCGAAACATTGAGCAGGTCTGTCAACAGGCTGACATTG
TGGTGGCGGCTGTGGGCAAAGCCAATATGATTAACGCATCGTATTTAAAAGCCGATGCCGTAGTGATTGATGTGGGCATT
AATCGAATTATGACACATGAAAAAACACGCTTGGTGGGTGATGTCGATTTTGAAGATGCCTTGCCAATGGTCAGTGCGAT
TACGCCTGTACCTGGTGGTGTTGGCCCAATGACCATTGCGTATTTGATGAAAAATACCTTGCAGGCGGTACGTTTGCAGC
ATCCACAAATATAA

Upstream 100 bases:

>100_bases
CTGTGGGACGTGATACCGAAACCGTCGCACTTTCTAAAGCAGTGAAATACTTTGTAGAGCATCGCGTCTTTATGAATGAT
GATCGTACGGTGGTGTTCAA

Downstream 100 bases:

>100_bases
ATGATGAAAAGGCGTTATGACACGCCGCGGAGAAATACACCATGCCTTTGAGTCTGCTGAAGTATGGATTAAGTGATGAG
TATCCTGTGGAAGTGGATTT

Product: bifunctional protein 5,10-methylene-tetrahydrofolate dehydrogenase/5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MSLALLQQHSPKIIDGKAIAQSVLEEVKLEVERYKAQGIEPCLAVVLVGEDPASQVYVRNKVQKAAFVGIRSLEFRFDAD
LSEDELLTQIDRLNTERGVHGILVQLPLPKQINETRVLERIHPQKDVDGFHSENVGGLSQGRDVLTPCTPTGCIRLLKDS
LGDDLSGLHAVVIGRSNIVGKPMAALLLKESCTVTVVHSKTRNIEQVCQQADIVVAAVGKANMINASYLKADAVVIDVGI
NRIMTHEKTRLVGDVDFEDALPMVSAITPVPGGVGPMTIAYLMKNTLQAVRLQHPQI

Sequences:

>Translated_297_residues
MSLALLQQHSPKIIDGKAIAQSVLEEVKLEVERYKAQGIEPCLAVVLVGEDPASQVYVRNKVQKAAFVGIRSLEFRFDAD
LSEDELLTQIDRLNTERGVHGILVQLPLPKQINETRVLERIHPQKDVDGFHSENVGGLSQGRDVLTPCTPTGCIRLLKDS
LGDDLSGLHAVVIGRSNIVGKPMAALLLKESCTVTVVHSKTRNIEQVCQQADIVVAAVGKANMINASYLKADAVVIDVGI
NRIMTHEKTRLVGDVDFEDALPMVSAITPVPGGVGPMTIAYLMKNTLQAVRLQHPQI
>Mature_296_residues
SLALLQQHSPKIIDGKAIAQSVLEEVKLEVERYKAQGIEPCLAVVLVGEDPASQVYVRNKVQKAAFVGIRSLEFRFDADL
SEDELLTQIDRLNTERGVHGILVQLPLPKQINETRVLERIHPQKDVDGFHSENVGGLSQGRDVLTPCTPTGCIRLLKDSL
GDDLSGLHAVVIGRSNIVGKPMAALLLKESCTVTVVHSKTRNIEQVCQQADIVVAAVGKANMINASYLKADAVVIDVGIN
RIMTHEKTRLVGDVDFEDALPMVSAITPVPGGVGPMTIAYLMKNTLQAVRLQHPQI

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI222136639, Length=289, Percent_Identity=41.1764705882353, Blast_Score=216, Evalue=2e-56,
Organism=Homo sapiens, GI94721354, Length=293, Percent_Identity=46.0750853242321, Blast_Score=210, Evalue=1e-54,
Organism=Homo sapiens, GI222418558, Length=300, Percent_Identity=42.6666666666667, Blast_Score=200, Evalue=1e-51,
Organism=Homo sapiens, GI36796743, Length=224, Percent_Identity=25.4464285714286, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1786741, Length=287, Percent_Identity=52.9616724738676, Blast_Score=265, Evalue=2e-72,
Organism=Caenorhabditis elegans, GI17568735, Length=288, Percent_Identity=38.1944444444444, Blast_Score=190, Evalue=7e-49,
Organism=Saccharomyces cerevisiae, GI6319558, Length=288, Percent_Identity=43.4027777777778, Blast_Score=233, Evalue=3e-62,
Organism=Saccharomyces cerevisiae, GI6321643, Length=297, Percent_Identity=42.0875420875421, Blast_Score=217, Evalue=2e-57,
Organism=Saccharomyces cerevisiae, GI6322933, Length=329, Percent_Identity=26.1398176291793, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI62472483, Length=292, Percent_Identity=44.5205479452055, Blast_Score=229, Evalue=2e-60,
Organism=Drosophila melanogaster, GI45551871, Length=292, Percent_Identity=44.5205479452055, Blast_Score=229, Evalue=2e-60,
Organism=Drosophila melanogaster, GI24645718, Length=286, Percent_Identity=45.4545454545455, Blast_Score=229, Evalue=2e-60,
Organism=Drosophila melanogaster, GI17137370, Length=286, Percent_Identity=45.4545454545455, Blast_Score=229, Evalue=2e-60,
Organism=Drosophila melanogaster, GI17136816, Length=295, Percent_Identity=46.1016949152542, Blast_Score=216, Evalue=2e-56,
Organism=Drosophila melanogaster, GI17136818, Length=295, Percent_Identity=46.1016949152542, Blast_Score=216, Evalue=2e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD1_ACIAD (Q6F9E6)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_047140.1
- HSSP:   P24186
- ProteinModelPortal:   Q6F9E6
- SMR:   Q6F9E6
- STRING:   Q6F9E6
- GeneID:   2878591
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD2553
- NMPDR:   fig|62977.3.peg.2333
- eggNOG:   COG0190
- HOGENOM:   HBG328751
- OMA:   CLMMLRD
- PhylomeDB:   Q6F9E6
- BioCyc:   ASP62977:ACIAD2553-MONOMER
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 32299; Mature: 32168

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLALLQQHSPKIIDGKAIAQSVLEEVKLEVERYKAQGIEPCLAVVLVGEDPASQVYVRN
CCCHHHHCCCCCEECHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHH
KVQKAAFVGIRSLEFRFDADLSEDELLTQIDRLNTERGVHGILVQLPLPKQINETRVLER
HHHHHHHHHHEEEEEEECCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHH
IHPQKDVDGFHSENVGGLSQGRDVLTPCTPTGCIRLLKDSLGDDLSGLHAVVIGRSNIVG
HCCCCCCCCCCCCCCCCHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCC
KPMAALLLKESCTVTVVHSKTRNIEQVCQQADIVVAAVGKANMINASYLKADAVVIDVGI
CHHHHHHHHCCCEEEEEECCCCCHHHHHHHCCEEEEECCCCCCCCHHHEEECEEEEECCH
NRIMTHEKTRLVGDVDFEDALPMVSAITPVPGGVGPMTIAYLMKNTLQAVRLQHPQI
HHHHHHHHHHEECCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SLALLQQHSPKIIDGKAIAQSVLEEVKLEVERYKAQGIEPCLAVVLVGEDPASQVYVRN
CCHHHHCCCCCEECHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHH
KVQKAAFVGIRSLEFRFDADLSEDELLTQIDRLNTERGVHGILVQLPLPKQINETRVLER
HHHHHHHHHHEEEEEEECCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHH
IHPQKDVDGFHSENVGGLSQGRDVLTPCTPTGCIRLLKDSLGDDLSGLHAVVIGRSNIVG
HCCCCCCCCCCCCCCCCHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCC
KPMAALLLKESCTVTVVHSKTRNIEQVCQQADIVVAAVGKANMINASYLKADAVVIDVGI
CHHHHHHHHCCCEEEEEECCCCCHHHHHHHCCEEEEECCCCCCCCHHHEEECEEEEECCH
NRIMTHEKTRLVGDVDFEDALPMVSAITPVPGGVGPMTIAYLMKNTLQAVRLQHPQI
HHHHHHHHHHEECCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA