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The map label for this gene is purU [H]

Identifier: 50085631

GI number: 50085631

Start: 2514022

End: 2514888

Strand: Reverse

Name: purU [H]

Synonym: ACIAD2554

Alternate gene names: 50085631

Gene position: 2514888-2514022 (Counterclockwise)

Preceding gene: 50085632

Following gene: 50085630

Centisome position: 69.88

GC content: 41.06

Gene sequence:

>867_bases
ATGGCTAACGATCTTGTCGATCAGTATGTTCTAAAAATCTCTTGTCCTGCTGCCTCAGGTATTGTGGCTGCTGTGTCAAA
TTGTCTTGCTGGAAATGATTGCTACATTAGCGAGATGGCACAATATGATGATGAAATTACAGGACGTTTCTTTAGCCGTA
TTGTATTTCGTTTCAATGAAGGTCAGGGCGATTTAGAGTTACTCAAAGAAAAATTTGCAGATGTCACTGCCAAGTTTGCA
ATGAAGGCAGATTTTTACGAAAAGAAAAATCCAATGAAAGTGCTGATCATGGTCAGCAAATTTGATCACTGTTTATTGAA
CTTGCTATATCGACATCATAAAGGTGAGCTGGATTTCCAGATTACTGCCATTGTATCGAATCATCTGGATTTACGTGCGA
TTGCCGAGCGCGAAGGGATTCGCTTCATTTATTTGCCCGTAAGTAAAGACACCAAGCAACAACAAGAACAAGAATTGCTG
AAGATCGTGGATGAAACCAAAACTGAACTGGTGATTTTAGCGCGCTACATGCAGATCTTATCGAATAACTTATGTACTCA
ATTATCTGGACGCGCAATCAATATTCATCATTCATTTTTGCCAGGGTTCAAAGGCGCTAAACCTTACCATCAAGCCTTTG
AACGAGGTGTCAAACTGATTGGTGCGACCGCACACTTTGTCACCAGTGATTTAGATGAAGGTCCGATTATTGAGCAGGAA
GTACAACGTGTAGATCATGCCTATATGCCAGATGATTTGGTCTCTGTGGGACGTGATACCGAAACCGTCGCACTTTCTAA
AGCAGTGAAATACTTTGTAGAGCATCGCGTCTTTATGAATGATGATCGTACGGTGGTGTTCAAATGA

Upstream 100 bases:

>100_bases
CAAAAGGAAAATAAATTTCTTAACAGTAAACTTACCGATGTCTTTAGATTTCTTTCACTACATCGCTAAGTGTCTTGAAT
CTGTACATTGAGGAAATATC

Downstream 100 bases:

>100_bases
GTCTTGCCTTATTACAACAGCACAGTCCCAAAATTATTGATGGTAAAGCCATTGCTCAAAGCGTACTTGAGGAAGTCAAG
CTAGAAGTTGAGCGTTATAA

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 288; Mature: 287

Protein sequence:

>288_residues
MANDLVDQYVLKISCPAASGIVAAVSNCLAGNDCYISEMAQYDDEITGRFFSRIVFRFNEGQGDLELLKEKFADVTAKFA
MKADFYEKKNPMKVLIMVSKFDHCLLNLLYRHHKGELDFQITAIVSNHLDLRAIAEREGIRFIYLPVSKDTKQQQEQELL
KIVDETKTELVILARYMQILSNNLCTQLSGRAINIHHSFLPGFKGAKPYHQAFERGVKLIGATAHFVTSDLDEGPIIEQE
VQRVDHAYMPDDLVSVGRDTETVALSKAVKYFVEHRVFMNDDRTVVFK

Sequences:

>Translated_288_residues
MANDLVDQYVLKISCPAASGIVAAVSNCLAGNDCYISEMAQYDDEITGRFFSRIVFRFNEGQGDLELLKEKFADVTAKFA
MKADFYEKKNPMKVLIMVSKFDHCLLNLLYRHHKGELDFQITAIVSNHLDLRAIAEREGIRFIYLPVSKDTKQQQEQELL
KIVDETKTELVILARYMQILSNNLCTQLSGRAINIHHSFLPGFKGAKPYHQAFERGVKLIGATAHFVTSDLDEGPIIEQE
VQRVDHAYMPDDLVSVGRDTETVALSKAVKYFVEHRVFMNDDRTVVFK
>Mature_287_residues
ANDLVDQYVLKISCPAASGIVAAVSNCLAGNDCYISEMAQYDDEITGRFFSRIVFRFNEGQGDLELLKEKFADVTAKFAM
KADFYEKKNPMKVLIMVSKFDHCLLNLLYRHHKGELDFQITAIVSNHLDLRAIAEREGIRFIYLPVSKDTKQQQEQELLK
IVDETKTELVILARYMQILSNNLCTQLSGRAINIHHSFLPGFKGAKPYHQAFERGVKLIGATAHFVTSDLDEGPIIEQEV
QRVDHAYMPDDLVSVGRDTETVALSKAVKYFVEHRVFMNDDRTVVFK

Specific function: Produces Formate From Formyl-Tetrahydrofolate. Provides The Major Source Of Formate For The Purt-Dependent Synthesis Of 5'-Phosphoribosyl-N-Formylglycinamide (Fgar) During Aerobic Growth. Has A Role In Regulating The One-Carbon Pool. [C]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=197, Percent_Identity=32.48730964467, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI209869995, Length=197, Percent_Identity=32.48730964467, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI209869993, Length=197, Percent_Identity=32.48730964467, Blast_Score=88, Evalue=1e-17,
Organism=Escherichia coli, GI1787483, Length=283, Percent_Identity=38.86925795053, Blast_Score=201, Evalue=4e-53,
Organism=Escherichia coli, GI1788846, Length=194, Percent_Identity=27.8350515463918, Blast_Score=83, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17567511, Length=205, Percent_Identity=30.2439024390244, Blast_Score=88, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 32760; Mature: 32629

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANDLVDQYVLKISCPAASGIVAAVSNCLAGNDCYISEMAQYDDEITGRFFSRIVFRFNE
CCCHHHHHHHHEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
GQGDLELLKEKFADVTAKFAMKADFYEKKNPMKVLIMVSKFDHCLLNLLYRHHKGELDFQ
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHCCCCCCEE
ITAIVSNHLDLRAIAEREGIRFIYLPVSKDTKQQQEQELLKIVDETKTELVILARYMQIL
EEEEECCCCHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SNNLCTQLSGRAINIHHSFLPGFKGAKPYHQAFERGVKLIGATAHFVTSDLDEGPIIEQE
HCCHHHHHCCCEEEEEHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
VQRVDHAYMPDDLVSVGRDTETVALSKAVKYFVEHRVFMNDDRTVVFK
HHHHHHCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHEECCCCEEEEC
>Mature Secondary Structure 
ANDLVDQYVLKISCPAASGIVAAVSNCLAGNDCYISEMAQYDDEITGRFFSRIVFRFNE
CCHHHHHHHHEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
GQGDLELLKEKFADVTAKFAMKADFYEKKNPMKVLIMVSKFDHCLLNLLYRHHKGELDFQ
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHCCCCCCEE
ITAIVSNHLDLRAIAEREGIRFIYLPVSKDTKQQQEQELLKIVDETKTELVILARYMQIL
EEEEECCCCHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SNNLCTQLSGRAINIHHSFLPGFKGAKPYHQAFERGVKLIGATAHFVTSDLDEGPIIEQE
HCCHHHHHCCCEEEEEHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
VQRVDHAYMPDDLVSVGRDTETVALSKAVKYFVEHRVFMNDDRTVVFK
HHHHHHCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHEECCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7543100 [H]