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The map label for this gene is purD [H]

Identifier: 50085532

GI number: 50085532

Start: 2412387

End: 2413694

Strand: Direct

Name: purD [H]

Synonym: ACIAD2448

Alternate gene names: 50085532

Gene position: 2412387-2413694 (Clockwise)

Preceding gene: 50085531

Following gene: 50085533

Centisome position: 67.04

GC content: 45.03

Gene sequence:

>1308_bases
CTGGATTCATGGAGTATGAACCCAATGAATATTCTCGTTCTCGGTAGCGGTGGTCGTGAACATGCACTTGCATGGAAAAT
CGCACAAGATGCACAAGTGACTCAGGTTTTTGTTGCGCCAGGCAATGCAGGTACTGCAACTGAAGACAAATGCAAGAATG
TTGAACTCGATATTTTAGATAATCCAGCCATCATTGAATTTGCACAATCGAATCAGATTGAATTGATTATTGTGGGGCCA
GAAGCTCCCCTTGTAAATGGCGTGGTCAATGCATGCCGTGAAGCGGATTTAAAAATCTGGGGTCCAACACAATATGCTGC
CCAACTTGAAGGCTCAAAAGCATTTGCTAAAGATTTTCTAAAAAAACATCAGATTCCAACTGCATTTTATGATGTATTCA
CCGAAGTTGATGCAGCGAAAGCTTATATAGAACAACATGGTGCACCTATTGTAATTAAGGCAGATGGCCTTGCAGCAGGG
AAAGGCGTCATCGTTGCGATGTCTAATGACGAAGCATTTGCTGCAATTGATGACATGCTTGCTGGCAATAAATTTGGTGA
TGCGGGTTCACGTGTCGTGATCGAAGAGTTTCTCGCAGGCGAAGAAGCCAGCTTTATTTGCATGATTGACGGTCAAAACA
TCTTACCAATGGCAACTTCGCAAGATCATAAACGTATCTTTGAAGCTGACCAAGGTCCAAATACGGGCGGTATGGGTGCA
TATTCCCCTGCTCCTGTGGTAACAGCAGACGTATTTGAACGCGTGATGAATGAAATCATGCGTCCTACAGTCGATGGCAT
GGCTCAAGATGGTCACGTCTATACAGGCTTTTTGTATGCGGGTCTTATGATTGATGAAAGCGGTCAACCGCGTGTGATTG
AGTTTAACTGTCGCTTCGGTGATCCAGAAACTCAACCGATCATGATGCGTTTGAAGTCATCTTTGGTTGATCTGGTACAA
GCAGGCCTTGAAGGTCAGCTTCCAAGTGAAGCCGAGTGGGATTCCCGCAAGTCAATTGGAATCGTATTGGCTGCAGAAGG
CTATCCTGAAACGGTACGTAAAGGCGATGTCATCTCTGGTTTAGGACAGTCGCCTGAAGATACCAAAATCTTTCATGCTG
GCACAGCAACCACAGAAGATGGTCATATCATCACCGCAGGTGGTCGTGTACTTTGCGTAACAGCATTGGGGGATACAGTA
CTTGAAGCTCAAATTAATGCGCTTGAAGTATGTGGACAAGTGACCTTTACAGGCATGCAATATCGTAGCGATATTGGTTA
CCGTGCGATTGCACGCGAACAAGCTTAA

Upstream 100 bases:

>100_bases
AAAGCACAAGAAACTTTCCGCGCTTTAGTTTTGTTTTATTACCCTCTCTTATAGAGAGGGTCTTGTACAAAAGAAATAAA
CTAAAAGTCAGGATTAGGTT

Downstream 100 bases:

>100_bases
CCACATTCTGATTATGAAGAAACGCGCATCTTTGCGCGTTTCTTCATTTAAATTACTATTTATCCATAGTCCGGTTCTAA
AAAAGTATTTCGAAAAAACT

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MDSWSMNPMNILVLGSGGREHALAWKIAQDAQVTQVFVAPGNAGTATEDKCKNVELDILDNPAIIEFAQSNQIELIIVGP
EAPLVNGVVNACREADLKIWGPTQYAAQLEGSKAFAKDFLKKHQIPTAFYDVFTEVDAAKAYIEQHGAPIVIKADGLAAG
KGVIVAMSNDEAFAAIDDMLAGNKFGDAGSRVVIEEFLAGEEASFICMIDGQNILPMATSQDHKRIFEADQGPNTGGMGA
YSPAPVVTADVFERVMNEIMRPTVDGMAQDGHVYTGFLYAGLMIDESGQPRVIEFNCRFGDPETQPIMMRLKSSLVDLVQ
AGLEGQLPSEAEWDSRKSIGIVLAAEGYPETVRKGDVISGLGQSPEDTKIFHAGTATTEDGHIITAGGRVLCVTALGDTV
LEAQINALEVCGQVTFTGMQYRSDIGYRAIAREQA

Sequences:

>Translated_435_residues
MDSWSMNPMNILVLGSGGREHALAWKIAQDAQVTQVFVAPGNAGTATEDKCKNVELDILDNPAIIEFAQSNQIELIIVGP
EAPLVNGVVNACREADLKIWGPTQYAAQLEGSKAFAKDFLKKHQIPTAFYDVFTEVDAAKAYIEQHGAPIVIKADGLAAG
KGVIVAMSNDEAFAAIDDMLAGNKFGDAGSRVVIEEFLAGEEASFICMIDGQNILPMATSQDHKRIFEADQGPNTGGMGA
YSPAPVVTADVFERVMNEIMRPTVDGMAQDGHVYTGFLYAGLMIDESGQPRVIEFNCRFGDPETQPIMMRLKSSLVDLVQ
AGLEGQLPSEAEWDSRKSIGIVLAAEGYPETVRKGDVISGLGQSPEDTKIFHAGTATTEDGHIITAGGRVLCVTALGDTV
LEAQINALEVCGQVTFTGMQYRSDIGYRAIAREQA
>Mature_435_residues
MDSWSMNPMNILVLGSGGREHALAWKIAQDAQVTQVFVAPGNAGTATEDKCKNVELDILDNPAIIEFAQSNQIELIIVGP
EAPLVNGVVNACREADLKIWGPTQYAAQLEGSKAFAKDFLKKHQIPTAFYDVFTEVDAAKAYIEQHGAPIVIKADGLAAG
KGVIVAMSNDEAFAAIDDMLAGNKFGDAGSRVVIEEFLAGEEASFICMIDGQNILPMATSQDHKRIFEADQGPNTGGMGA
YSPAPVVTADVFERVMNEIMRPTVDGMAQDGHVYTGFLYAGLMIDESGQPRVIEFNCRFGDPETQPIMMRLKSSLVDLVQ
AGLEGQLPSEAEWDSRKSIGIVLAAEGYPETVRKGDVISGLGQSPEDTKIFHAGTATTEDGHIITAGGRVLCVTALGDTV
LEAQINALEVCGQVTFTGMQYRSDIGYRAIAREQA

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI28416899, Length=429, Percent_Identity=47.7855477855478, Blast_Score=400, Evalue=1e-111,
Organism=Homo sapiens, GI4503915, Length=429, Percent_Identity=47.7855477855478, Blast_Score=400, Evalue=1e-111,
Organism=Homo sapiens, GI209869995, Length=429, Percent_Identity=47.7855477855478, Blast_Score=400, Evalue=1e-111,
Organism=Homo sapiens, GI209869993, Length=429, Percent_Identity=47.7855477855478, Blast_Score=400, Evalue=1e-111,
Organism=Escherichia coli, GI1790438, Length=428, Percent_Identity=64.018691588785, Blast_Score=543, Evalue=1e-156,
Organism=Caenorhabditis elegans, GI17567511, Length=425, Percent_Identity=41.8823529411765, Blast_Score=305, Evalue=2e-83,
Organism=Saccharomyces cerevisiae, GI6321203, Length=446, Percent_Identity=42.6008968609865, Blast_Score=345, Evalue=1e-95,
Organism=Drosophila melanogaster, GI62471619, Length=430, Percent_Identity=43.7209302325581, Blast_Score=342, Evalue=2e-94,
Organism=Drosophila melanogaster, GI24582400, Length=430, Percent_Identity=43.7209302325581, Blast_Score=342, Evalue=3e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 46607; Mature: 46607

Theoretical pI: Translated: 4.28; Mature: 4.28

Prosite motif: PS50975 ATP_GRASP ; PS00178 AA_TRNA_LIGASE_I ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSWSMNPMNILVLGSGGREHALAWKIAQDAQVTQVFVAPGNAGTATEDKCKNVELDILD
CCCCCCCCEEEEEECCCCCCCEEEEEECCCCEEEEEEEECCCCCCCCCHHHCCEEEEEEC
NPAIIEFAQSNQIELIIVGPEAPLVNGVVNACREADLKIWGPTQYAAQLEGSKAFAKDFL
CCCEEEEECCCCEEEEEECCCCCHHHHHHHHHHHCCEEEECCCHHHHHCCCCHHHHHHHH
KKHQIPTAFYDVFTEVDAAKAYIEQHGAPIVIKADGLAAGKGVIVAMSNDEAFAAIDDML
HHCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEECCCCCHHHHHHHH
AGNKFGDAGSRVVIEEFLAGEEASFICMIDGQNILPMATSQDHKRIFEADQGPNTGGMGA
CCCCCCCCCCHHHHHHHHCCCCCCEEEEECCCCEEECCCCCHHHHHHCCCCCCCCCCCCC
YSPAPVVTADVFERVMNEIMRPTVDGMAQDGHVYTGFLYAGLMIDESGQPRVIEFNCRFG
CCCCCEEHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEEECCCCCCEEEEEEECCC
DPETQPIMMRLKSSLVDLVQAGLEGQLPSEAEWDSRKSIGIVLAAEGYPETVRKGDVISG
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCHHHHCCCHHHC
LGQSPEDTKIFHAGTATTEDGHIITAGGRVLCVTALGDTVLEAQINALEVCGQVTFTGMQ
CCCCCCCCEEEECCCCCCCCCEEEEECCCEEEEEECCCHHHHHHHHHHHHHCCEEEECEE
YRSDIGYRAIAREQA
HHCCCCHHHHHCCCC
>Mature Secondary Structure
MDSWSMNPMNILVLGSGGREHALAWKIAQDAQVTQVFVAPGNAGTATEDKCKNVELDILD
CCCCCCCCEEEEEECCCCCCCEEEEEECCCCEEEEEEEECCCCCCCCCHHHCCEEEEEEC
NPAIIEFAQSNQIELIIVGPEAPLVNGVVNACREADLKIWGPTQYAAQLEGSKAFAKDFL
CCCEEEEECCCCEEEEEECCCCCHHHHHHHHHHHCCEEEECCCHHHHHCCCCHHHHHHHH
KKHQIPTAFYDVFTEVDAAKAYIEQHGAPIVIKADGLAAGKGVIVAMSNDEAFAAIDDML
HHCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEECCCCCHHHHHHHH
AGNKFGDAGSRVVIEEFLAGEEASFICMIDGQNILPMATSQDHKRIFEADQGPNTGGMGA
CCCCCCCCCCHHHHHHHHCCCCCCEEEEECCCCEEECCCCCHHHHHHCCCCCCCCCCCCC
YSPAPVVTADVFERVMNEIMRPTVDGMAQDGHVYTGFLYAGLMIDESGQPRVIEFNCRFG
CCCCCEEHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEEECCCCCCEEEEEEECCC
DPETQPIMMRLKSSLVDLVQAGLEGQLPSEAEWDSRKSIGIVLAAEGYPETVRKGDVISG
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCHHHHCCCHHHC
LGQSPEDTKIFHAGTATTEDGHIITAGGRVLCVTALGDTVLEAQINALEVCGQVTFTGMQ
CCCCCCCCEEEECCCCCCCCCEEEEECCCEEEEEECCCHHHHHHHHHHHHHCCEEEECEE
YRSDIGYRAIAREQA
HHCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]