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The map label for this gene is purH [H]

Identifier: 50085531

GI number: 50085531

Start: 2410701

End: 2412275

Strand: Direct

Name: purH [H]

Synonym: ACIAD2447

Alternate gene names: 50085531

Gene position: 2410701-2412275 (Clockwise)

Preceding gene: 50085530

Following gene: 50085532

Centisome position: 66.99

GC content: 42.92

Gene sequence:

>1575_bases
ATGACTATTAAACGTGCTTTGATTTCTGTTTCCGACAAAACAGGAATTGTAGAATTTGCTCAAAATCTTGCGAAACTTGG
GGTAGAAATTTTATCTACTGGTGGCACTTACAAGTTGCTCAAAGACAATAATATTGCCGTAGTGGAAGTTTCTGAACATA
CTGGCTTTCCTGAAATGATGGATGGTCGTGTAAAAACACTTCACCCTAAAATCCATGGTGGTATTCTAGCTCGCCGCGGT
CTTGATGAAGCTGTTATGCAAGAACACAATATCGATCCGATTGATCTGGTTGTTGTGAATCTCTATCCTTTTGCTGCAAC
TGTGGCAAAACCGAATTGCTCACTTGCAGACGCCATTGAAAATATCGACATTGGTGGCCCGACGATGGTTCGTGCTGCTG
CAAAAAATCATGCCTCTGTTGGCATCGTCGTTAACGCAAGTGATTACGATACCATTATCGCTGAGCTTGAGCAAAATGGT
GGTTTAAGTTACGAAACTCGTTTTGATCTTGCAGTAAAAGCATTTGAACATACTGCTCAATACGACGGTATGATCGCTTC
TTATTTAGGTGCGCGTGTTGGTAAAGCCGAAGGTGAAGCGGACTTGTTCCCTCGTACTTTTAATACGCAACTGGTCAAGG
CTCAGGACTTACGTTACGGTGAAAATCCGCATCAGTCGGCTGCCTTCTATGTTGATTCAAATGCATCTGAAGCATCTGTT
TCAACCTCTAAACAGCTTCAAGGTAAAGAGTTATCTTATAACAACATTGCAGATACAGATGCAGCACTTGAATGCGTAAA
ATCATTTGCAAAACCTGCGTGTGTGATTGTGAAACATGCCAACCCATGTGGTGTTGCTGTTTCTCTTGATGGTATTAAAG
CAGCTTACGACCTGGCTTATGCTACAGATACAGAATCTGCTTTTGGTGGCATTATTGCATTCAACCGTGAACTCGATGTT
GAAACAGCACAAGCCATTGTTGATCGTCAGTTTGTCGAAGTTATTATTGCACCTAGCATTGCTGATGGCGTACTTGAGGT
TACTGGTGCGAAGAAAAATGTTCGCGTACTCGTATGTGGTGAACTTCCAGCAATTGATGAACGTGCAGCACAACTCGACT
ACAAACGTGTCAATGGTGGACTGCTCGTTCAAGATCAAGATCTTGGTATGATCAAGGCAGCTGACCTTAAAGTTGTCACT
GAACTTGCACCAACAGAGCAAGAAATTGACGACCTGATCTTTGCATGGAAAGTGGCTAAGTATGTGAAATCAAATGCCAT
TGTTTATGCAAAAAATCGCCAAACCATTGGTGTGGGTGCCGGCCAAATGAGTCGTGTTAACTCTGCTCGTATTGCAGCAA
TCAAAGCTGAACATGCAGGTTTAGTGGTTGAAGGTGCAGTTATGGCATCAGATGCATTTTTCCCGTTCCGCGATGGAATT
GATAATGCAGCGAAAGCAGGAATTAAGTGTATTATTCAACCTGGTGGTTCTATGCGTGATGATGAAGTCATTGCTGCTGC
GAACGAACATGGGATTGCCATGGTATTCACCGGAATGCGTCATTTCCGTCATTAA

Upstream 100 bases:

>100_bases
CGGATTGATGAGTGAATAAATGATAAAATGCTCACGTCCTCGTGGGCATTTTTTATGCCTATTTGTTTTAATCTGTTGAA
CCTAAACCGTTGACTTAATC

Downstream 100 bases:

>100_bases
TCAATATCCTTAAAGCACAAGAAACTTTCCGCGCTTTAGTTTTGTTTTATTACCCTCTCTTATAGAGAGGGTCTTGTACA
AAAGAAATAAACTAAAAGTC

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 524; Mature: 523

Protein sequence:

>524_residues
MTIKRALISVSDKTGIVEFAQNLAKLGVEILSTGGTYKLLKDNNIAVVEVSEHTGFPEMMDGRVKTLHPKIHGGILARRG
LDEAVMQEHNIDPIDLVVVNLYPFAATVAKPNCSLADAIENIDIGGPTMVRAAAKNHASVGIVVNASDYDTIIAELEQNG
GLSYETRFDLAVKAFEHTAQYDGMIASYLGARVGKAEGEADLFPRTFNTQLVKAQDLRYGENPHQSAAFYVDSNASEASV
STSKQLQGKELSYNNIADTDAALECVKSFAKPACVIVKHANPCGVAVSLDGIKAAYDLAYATDTESAFGGIIAFNRELDV
ETAQAIVDRQFVEVIIAPSIADGVLEVTGAKKNVRVLVCGELPAIDERAAQLDYKRVNGGLLVQDQDLGMIKAADLKVVT
ELAPTEQEIDDLIFAWKVAKYVKSNAIVYAKNRQTIGVGAGQMSRVNSARIAAIKAEHAGLVVEGAVMASDAFFPFRDGI
DNAAKAGIKCIIQPGGSMRDDEVIAAANEHGIAMVFTGMRHFRH

Sequences:

>Translated_524_residues
MTIKRALISVSDKTGIVEFAQNLAKLGVEILSTGGTYKLLKDNNIAVVEVSEHTGFPEMMDGRVKTLHPKIHGGILARRG
LDEAVMQEHNIDPIDLVVVNLYPFAATVAKPNCSLADAIENIDIGGPTMVRAAAKNHASVGIVVNASDYDTIIAELEQNG
GLSYETRFDLAVKAFEHTAQYDGMIASYLGARVGKAEGEADLFPRTFNTQLVKAQDLRYGENPHQSAAFYVDSNASEASV
STSKQLQGKELSYNNIADTDAALECVKSFAKPACVIVKHANPCGVAVSLDGIKAAYDLAYATDTESAFGGIIAFNRELDV
ETAQAIVDRQFVEVIIAPSIADGVLEVTGAKKNVRVLVCGELPAIDERAAQLDYKRVNGGLLVQDQDLGMIKAADLKVVT
ELAPTEQEIDDLIFAWKVAKYVKSNAIVYAKNRQTIGVGAGQMSRVNSARIAAIKAEHAGLVVEGAVMASDAFFPFRDGI
DNAAKAGIKCIIQPGGSMRDDEVIAAANEHGIAMVFTGMRHFRH
>Mature_523_residues
TIKRALISVSDKTGIVEFAQNLAKLGVEILSTGGTYKLLKDNNIAVVEVSEHTGFPEMMDGRVKTLHPKIHGGILARRGL
DEAVMQEHNIDPIDLVVVNLYPFAATVAKPNCSLADAIENIDIGGPTMVRAAAKNHASVGIVVNASDYDTIIAELEQNGG
LSYETRFDLAVKAFEHTAQYDGMIASYLGARVGKAEGEADLFPRTFNTQLVKAQDLRYGENPHQSAAFYVDSNASEASVS
TSKQLQGKELSYNNIADTDAALECVKSFAKPACVIVKHANPCGVAVSLDGIKAAYDLAYATDTESAFGGIIAFNRELDVE
TAQAIVDRQFVEVIIAPSIADGVLEVTGAKKNVRVLVCGELPAIDERAAQLDYKRVNGGLLVQDQDLGMIKAADLKVVTE
LAPTEQEIDDLIFAWKVAKYVKSNAIVYAKNRQTIGVGAGQMSRVNSARIAAIKAEHAGLVVEGAVMASDAFFPFRDGID
NAAKAGIKCIIQPGGSMRDDEVIAAANEHGIAMVFTGMRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=516, Percent_Identity=38.5658914728682, Blast_Score=276, Evalue=2e-74,
Organism=Escherichia coli, GI1790439, Length=527, Percent_Identity=69.2599620493359, Blast_Score=747, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71985564, Length=511, Percent_Identity=36.5949119373777, Blast_Score=267, Evalue=1e-71,
Organism=Caenorhabditis elegans, GI71985574, Length=343, Percent_Identity=29.7376093294461, Blast_Score=102, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI71985556, Length=60, Percent_Identity=51.6666666666667, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6323768, Length=484, Percent_Identity=37.1900826446281, Blast_Score=268, Evalue=1e-72,
Organism=Saccharomyces cerevisiae, GI6323056, Length=487, Percent_Identity=36.5503080082135, Blast_Score=266, Evalue=7e-72,
Organism=Drosophila melanogaster, GI24649832, Length=511, Percent_Identity=37.573385518591, Blast_Score=280, Evalue=2e-75,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 56139; Mature: 56007

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIKRALISVSDKTGIVEFAQNLAKLGVEILSTGGTYKLLKDNNIAVVEVSEHTGFPEMM
CCCHHEEEEECCCCCHHHHHHHHHHHCHHEECCCCEEEEEECCCEEEEEECCCCCCHHHH
DGRVKTLHPKIHGGILARRGLDEAVMQEHNIDPIDLVVVNLYPFAATVAKPNCSLADAIE
CCCEEEECCHHHCCHHHHCCCCHHHHHHCCCCCEEEEEEEEECCEEEECCCCCCHHHHHH
NIDIGGPTMVRAAAKNHASVGIVVNASDYDTIIAELEQNGGLSYETRFDLAVKAFEHTAQ
CCCCCCCHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHH
YDGMIASYLGARVGKAEGEADLFPRTFNTQLVKAQDLRYGENPHQSAAFYVDSNASEASV
HCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCEEEEEECCCCCCCC
STSKQLQGKELSYNNIADTDAALECVKSFAKPACVIVKHANPCGVAVSLDGIKAAYDLAY
CHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCEEEEECCCCHHHEEEE
ATDTESAFGGIIAFNRELDVETAQAIVDRQFVEVIIAPSIADGVLEVTGAKKNVRVLVCG
ECCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHEEECCCCCCCEEEECCCCCCEEEEEEC
ELPAIDERAAQLDYKRVNGGLLVQDQDLGMIKAADLKVVTELAPTEQEIDDLIFAWKVAK
CCCCCCHHHHHCCCEECCCCEEEEECCCCEEEECCEEEEECCCCCHHHHHHHHHHHHHHH
YVKSNAIVYAKNRQTIGVGAGQMSRVNSARIAAIKAEHAGLVVEGAVMASDAFFPFRDGI
HHHCCEEEEEECCEEEEECCCCHHHCCCCEEEEEEECCCCEEEECEEEECCCCCCHHCCC
DNAAKAGIKCIIQPGGSMRDDEVIAAANEHGIAMVFTGMRHFRH
CHHHCCCEEEEECCCCCCCCCCEEEECCCCCEEEEEECHHHHCC
>Mature Secondary Structure 
TIKRALISVSDKTGIVEFAQNLAKLGVEILSTGGTYKLLKDNNIAVVEVSEHTGFPEMM
CCHHEEEEECCCCCHHHHHHHHHHHCHHEECCCCEEEEEECCCEEEEEECCCCCCHHHH
DGRVKTLHPKIHGGILARRGLDEAVMQEHNIDPIDLVVVNLYPFAATVAKPNCSLADAIE
CCCEEEECCHHHCCHHHHCCCCHHHHHHCCCCCEEEEEEEEECCEEEECCCCCCHHHHHH
NIDIGGPTMVRAAAKNHASVGIVVNASDYDTIIAELEQNGGLSYETRFDLAVKAFEHTAQ
CCCCCCCHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHH
YDGMIASYLGARVGKAEGEADLFPRTFNTQLVKAQDLRYGENPHQSAAFYVDSNASEASV
HCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCEEEEEECCCCCCCC
STSKQLQGKELSYNNIADTDAALECVKSFAKPACVIVKHANPCGVAVSLDGIKAAYDLAY
CHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCEEEEECCCCHHHEEEE
ATDTESAFGGIIAFNRELDVETAQAIVDRQFVEVIIAPSIADGVLEVTGAKKNVRVLVCG
ECCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHEEECCCCCCCEEEECCCCCCEEEEEEC
ELPAIDERAAQLDYKRVNGGLLVQDQDLGMIKAADLKVVTELAPTEQEIDDLIFAWKVAK
CCCCCCHHHHHCCCEECCCCEEEEECCCCEEEECCEEEEECCCCCHHHHHHHHHHHHHHH
YVKSNAIVYAKNRQTIGVGAGQMSRVNSARIAAIKAEHAGLVVEGAVMASDAFFPFRDGI
HHHCCEEEEEECCEEEEECCCCHHHCCCCEEEEEEECCCCEEEECEEEECCCCCCHHCCC
DNAAKAGIKCIIQPGGSMRDDEVIAAANEHGIAMVFTGMRHFRH
CHHHCCCEEEEECCCCCCCCCCEEEECCCCCEEEEEECHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA