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The map label for this gene is ykoW [H]

Identifier: 50085506

GI number: 50085506

Start: 2385310

End: 2386755

Strand: Reverse

Name: ykoW [H]

Synonym: ACIAD2421

Alternate gene names: 50085506

Gene position: 2386755-2385310 (Counterclockwise)

Preceding gene: 50085507

Following gene: 161349962

Centisome position: 66.32

GC content: 36.17

Gene sequence:

>1446_bases
ATGCGTATGAATCTGATGAATGCTCGTCATCTTGCTTACTACGATCTTGATTTAATGCCACATGCAACACAACTATCTAG
ACAGCCTCATCATACTCCTGTACTGGGTAGCCATAACGTGAATTTTCCAAGCTCAATTCCGCTTGCAGAACGCTCAGATA
AAAATAGTGAAATAGATCGACTTACTGGACTACCTAATCAGTCAGGATTTAAGAAGCAACTTCATACCCTCATTGCATCT
ATTCATCAGAAAAAAAATTACAATTTTGGATTGATCCTATTTGATTTTGACTATTTTAATCAGATCAATACTAGTCATGG
TCATATTGTGGGCGATCATATTTTACAAACAATTGCCTCAAGAATTAAAAAAGTATTACCACAAGAAGTGCTTTTATCGC
GTTTGGGAAGTGACGAGTTTGCGATTTTGATTCCTAATGTGTCTTCAGATATAAGTTTAATGAAGATAGGATCACTAGTG
CAGCAACAGGTTTCTTTACCTTTATATTATCGTGATCATATTATTGAAAGTAATATCAGTGCTGGAGGTGCAATTTATCC
TCGTGATATTAATCAAGACGTTAATATTGTTGAAGCGGCAGATATTGCATTGTGTTATTTGAAACAATCTGGTCGTGGTG
GATGTTCTATTTTTCGATCTGAAATGCTGGTTGAACAAAATATGATTGCCCAACAATTTAATCGTGCTAGACATATCTTA
CGTCATAATTTAGTGCGTCCCTTTTATCAGCCAAAATTTTATTTAGAAAGTGGCAAACTTGCAGGCTTTGAAGCATTACT
GCGCTGGTATGATGAAGATAATCAAATACAATTGCCAAAATTGATTATAAATGCGTTTGAAGACTATAGTCTTGCGAGTC
GTTTGAGCGAGAAGATGCAATTAAATATCTTTAAAGATATGGCAAGCTGGATCAATCAGGGTTTACAACTCGTTCCTGTA
TCCATCAATGCTGCGCCAGTTGAGTTTTTAAAGGACAACTATGCAGAATTATTATTAGAACGCTTAAATCAATTTGAGAT
TCCATATCATTTGATTGAAATTGAAATTACTGAACATTTGTTAGATGATCAAGGGAAGAACTTTATTTCCCGTGCACTGG
CTAAATTGAAGCAAAATGGTATTCGAATCGCTCTGGATGATTTTGGTACTGGCCATTCATCCTTGACTCGCCTCGCAAAT
TTGCCTGTAGATTGCCTAAAAATAGACTGTGATTTTGTACAACGTATGGAACAGGAAAAGTTTATTTCAGCACTCGTGCA
ATCTGTTATTTTAATGAGTAATAATCTCGGCATTGATGTGGTTGCAGAAGGTATTGAAAGTCAGGAACAGCTTGAATTAC
TAAAACAACAGGGGTGTGAAGTAGGACAGGGTTTTTTGTTTAGTCAGGCTTTAGCACCTGAAAACGCTCGTGCATTGTTG
TCATAA

Upstream 100 bases:

>100_bases
ATAAGTGTACAAACCAAATGTAAATTGCGTCACATTTTAGTTTTATAATTAACAAAAGCGTACATTTGGCTTATTTCTAT
TTTGTCTGTAAACAGGAGGG

Downstream 100 bases:

>100_bases
ATCAAATTATATGAAAGCGCTTTTATAAATCTGAACAGACTCTATAAAAACGCTATCCTTCTTGTTTAGGTCAAATAATA
ATTAAAGTGCGTTCTTGATA

Product: diguanylate cyclase/phosphodiesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 481; Mature: 481

Protein sequence:

>481_residues
MRMNLMNARHLAYYDLDLMPHATQLSRQPHHTPVLGSHNVNFPSSIPLAERSDKNSEIDRLTGLPNQSGFKKQLHTLIAS
IHQKKNYNFGLILFDFDYFNQINTSHGHIVGDHILQTIASRIKKVLPQEVLLSRLGSDEFAILIPNVSSDISLMKIGSLV
QQQVSLPLYYRDHIIESNISAGGAIYPRDINQDVNIVEAADIALCYLKQSGRGGCSIFRSEMLVEQNMIAQQFNRARHIL
RHNLVRPFYQPKFYLESGKLAGFEALLRWYDEDNQIQLPKLIINAFEDYSLASRLSEKMQLNIFKDMASWINQGLQLVPV
SINAAPVEFLKDNYAELLLERLNQFEIPYHLIEIEITEHLLDDQGKNFISRALAKLKQNGIRIALDDFGTGHSSLTRLAN
LPVDCLKIDCDFVQRMEQEKFISALVQSVILMSNNLGIDVVAEGIESQEQLELLKQQGCEVGQGFLFSQALAPENARALL
S

Sequences:

>Translated_481_residues
MRMNLMNARHLAYYDLDLMPHATQLSRQPHHTPVLGSHNVNFPSSIPLAERSDKNSEIDRLTGLPNQSGFKKQLHTLIAS
IHQKKNYNFGLILFDFDYFNQINTSHGHIVGDHILQTIASRIKKVLPQEVLLSRLGSDEFAILIPNVSSDISLMKIGSLV
QQQVSLPLYYRDHIIESNISAGGAIYPRDINQDVNIVEAADIALCYLKQSGRGGCSIFRSEMLVEQNMIAQQFNRARHIL
RHNLVRPFYQPKFYLESGKLAGFEALLRWYDEDNQIQLPKLIINAFEDYSLASRLSEKMQLNIFKDMASWINQGLQLVPV
SINAAPVEFLKDNYAELLLERLNQFEIPYHLIEIEITEHLLDDQGKNFISRALAKLKQNGIRIALDDFGTGHSSLTRLAN
LPVDCLKIDCDFVQRMEQEKFISALVQSVILMSNNLGIDVVAEGIESQEQLELLKQQGCEVGQGFLFSQALAPENARALL
S
>Mature_481_residues
MRMNLMNARHLAYYDLDLMPHATQLSRQPHHTPVLGSHNVNFPSSIPLAERSDKNSEIDRLTGLPNQSGFKKQLHTLIAS
IHQKKNYNFGLILFDFDYFNQINTSHGHIVGDHILQTIASRIKKVLPQEVLLSRLGSDEFAILIPNVSSDISLMKIGSLV
QQQVSLPLYYRDHIIESNISAGGAIYPRDINQDVNIVEAADIALCYLKQSGRGGCSIFRSEMLVEQNMIAQQFNRARHIL
RHNLVRPFYQPKFYLESGKLAGFEALLRWYDEDNQIQLPKLIINAFEDYSLASRLSEKMQLNIFKDMASWINQGLQLVPV
SINAAPVEFLKDNYAELLLERLNQFEIPYHLIEIEITEHLLDDQGKNFISRALAKLKQNGIRIALDDFGTGHSSLTRLAN
LPVDCLKIDCDFVQRMEQEKFISALVQSVILMSNNLGIDVVAEGIESQEQLELLKQQGCEVGQGFLFSQALAPENARALL
S

Specific function: Probable signaling protein whose physiological role is not yet known [H]

COG id: COG5001

COG function: function code T; Predicted signal transduction protein containing a membrane domain, an EAL and a GGDEF domain

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1787541, Length=420, Percent_Identity=28.8095238095238, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI87081921, Length=429, Percent_Identity=27.5058275058275, Blast_Score=171, Evalue=9e-44,
Organism=Escherichia coli, GI1790496, Length=251, Percent_Identity=29.8804780876494, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI87081743, Length=227, Percent_Identity=34.3612334801762, Blast_Score=128, Evalue=9e-31,
Organism=Escherichia coli, GI226510982, Length=429, Percent_Identity=24.009324009324, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI87081980, Length=233, Percent_Identity=31.7596566523605, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI87081845, Length=239, Percent_Identity=29.2887029288703, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1786507, Length=239, Percent_Identity=30.5439330543933, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788502, Length=225, Percent_Identity=30.6666666666667, Blast_Score=95, Evalue=9e-21,
Organism=Escherichia coli, GI1788381, Length=422, Percent_Identity=21.8009478672986, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI87082096, Length=214, Percent_Identity=26.1682242990654, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1787055, Length=257, Percent_Identity=26.8482490272374, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI1788849, Length=246, Percent_Identity=25.609756097561, Blast_Score=69, Evalue=8e-13,
Organism=Escherichia coli, GI1787802, Length=192, Percent_Identity=27.6041666666667, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1788956, Length=128, Percent_Identity=32.8125, Blast_Score=66, Evalue=6e-12,
Organism=Escherichia coli, GI1787410, Length=155, Percent_Identity=29.6774193548387, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR001633
- InterPro:   IPR005330
- InterPro:   IPR000014 [H]

Pfam domain/function: PF00563 EAL; PF00990 GGDEF; PF03707 MHYT [H]

EC number: NA

Molecular weight: Translated: 54494; Mature: 54494

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS50883 EAL ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRMNLMNARHLAYYDLDLMPHATQLSRQPHHTPVLGSHNVNFPSSIPLAERSDKNSEIDR
CCCCCCCCCEEEEEECCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
LTGLPNQSGFKKQLHTLIASIHQKKNYNFGLILFDFDYFNQINTSHGHIVGDHILQTIAS
HCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECHHHHCCCCCCCCCHHHHHHHHHHH
RIKKVLPQEVLLSRLGSDEFAILIPNVSSDISLMKIGSLVQQQVSLPLYYRDHIIESNIS
HHHHHCCHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCC
AGGAIYPRDINQDVNIVEAADIALCYLKQSGRGGCSIFRSEMLVEQNMIAQQFNRARHIL
CCCCCCCCCCCCCCCEEHHHHHHEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RHNLVRPFYQPKFYLESGKLAGFEALLRWYDEDNQIQLPKLIINAFEDYSLASRLSEKMQ
HHHHHCCCCCCCEEECCCCCHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHH
LNIFKDMASWINQGLQLVPVSINAAPVEFLKDNYAELLLERLNQFEIPYHLIEIEITEHL
HHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHHH
LDDQGKNFISRALAKLKQNGIRIALDDFGTGHSSLTRLANLPVDCLKIDCDFVQRMEQEK
HCCCCHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHCCCHHHEEECHHHHHHHHHHH
FISALVQSVILMSNNLGIDVVAEGIESQEQLELLKQQGCEVGQGFLFSQALAPENARALL
HHHHHHHHHHHHCCCCCHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHCCCCCHHHHC
S
C
>Mature Secondary Structure
MRMNLMNARHLAYYDLDLMPHATQLSRQPHHTPVLGSHNVNFPSSIPLAERSDKNSEIDR
CCCCCCCCCEEEEEECCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
LTGLPNQSGFKKQLHTLIASIHQKKNYNFGLILFDFDYFNQINTSHGHIVGDHILQTIAS
HCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECHHHHCCCCCCCCCHHHHHHHHHHH
RIKKVLPQEVLLSRLGSDEFAILIPNVSSDISLMKIGSLVQQQVSLPLYYRDHIIESNIS
HHHHHCCHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCC
AGGAIYPRDINQDVNIVEAADIALCYLKQSGRGGCSIFRSEMLVEQNMIAQQFNRARHIL
CCCCCCCCCCCCCCCEEHHHHHHEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RHNLVRPFYQPKFYLESGKLAGFEALLRWYDEDNQIQLPKLIINAFEDYSLASRLSEKMQ
HHHHHCCCCCCCEEECCCCCHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHH
LNIFKDMASWINQGLQLVPVSINAAPVEFLKDNYAELLLERLNQFEIPYHLIEIEITEHL
HHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHHH
LDDQGKNFISRALAKLKQNGIRIALDDFGTGHSSLTRLANLPVDCLKIDCDFVQRMEQEK
HCCCCHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHCCCHHHEEECHHHHHHHHHHH
FISALVQSVILMSNNLGIDVVAEGIESQEQLELLKQQGCEVGQGFLFSQALAPENARALL
HHHHHHHHHHHHCCCCCHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHCCCCCHHHHC
S
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 11728710 [H]