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The map label for this gene is prfA

Identifier: 161349962

GI number: 161349962

Start: 2382061

End: 2383149

Strand: Reverse

Name: prfA

Synonym: ACIAD2418

Alternate gene names: 161349962

Gene position: 2383149-2382061 (Counterclockwise)

Preceding gene: 50085506

Following gene: 50085502

Centisome position: 66.22

GC content: 44.08

Gene sequence:

>1089_bases
ATGAAAGAATCGCTACGTTTACGATTAGATCAATTGTGTGACCGTCATGAAGAATTAACAGCATTGCTAGCAGATGCTGA
AGTTATTTCAGACAATAAACGTTTTCGTAAATTATCACGTGAACATAGTGATTTAAATGAAATTGTTGACGTGTGGAGTA
AATATCGTCAAGCGGAAGAAGACATTGAAACAGCCGAGTCGATGCTGAGTGATCCTGACTTTAAGGATATGGCTCAAGAA
GAAATTAAAGAAAACCGTGCTTTGATTGAGCAGCTTGAAGGTGATCTGAACATTCTGATGATTCCTAAAGACCCCAATGA
CGCCAATGCGGCTTATCTGGAAGTTCGCGCAGGAACGGGGGGAGATGAAGCTGCTATTTTCTCAGGCGATTTATTTCGCA
TGTACAGTAAATATGCCGAGTCTCGGGGATGGCGCATTGAAGTACTTTCAGAAAATGAAGGTGAGCATGGCGGTTATAAG
GAAGTGATTTGTCGCGTTGATGGCGATGGTGTATATGGTCGTTTAAAATTTGAAAGTGGTGCACATCGTGTCCAACGTGT
TCCAGCCACCGAATCGCAAGGGCGTGTGCATACTTCGGCGTGTACTGTAGCAATTTTACCTGAGGTTGATGTCGATACGA
CTGTTGAAATTAATCCAGCCGATTTGCGTATTGATACTTATCGCGCCTCTGGTGCTGGTGGTCAGCACATTAACAAGACT
GACTCGGCTGTTCGTATCACACACGTACCTACGGGTGTGGTAGTTGAATGTCAAGAAGAGCGTTCTCAGCATAAAAACAA
GGCCAAGGCCATGGCTTTACTGGTGTCGCGTTTAGAAAATGCCAAACGTGCAGCACAGGAAACGGCGACTTCTGAAATGC
GTCGTGACTTGGTGGGATCTGGTGACCGTTCGGAACGTATCCGTACATATAACTACCCACAAGGACGAATGACTGATCAC
CGTATTAATCTAACACTGTATAAGTTAGATGCAATTATGGAAGGTGATTTGACTGAACTGCTGGATAGTTTACACCGTGA
ATATCAGGCTGATCAGTTGGCTATGTTGGCACAAGAGAATGGTGGTTAA

Upstream 100 bases:

>100_bases
ATGCGCAATTTTAGGCTTTAACCTGTACCTACATGACTGATTCATCATATTTGTTGTCATGAGTGAGAGCAGGAATTTAT
TTATTGAGATTTTTCTTCCT

Downstream 100 bases:

>100_bases
TGAATATCGGACAAGCACTCAACTTACGTGGTGAATCTGACAGTTATGAACGTCAGGAAGCAATGTGGTTGCTTGAACAT
ATTTTAGAGTTGAACAGTCT

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 362; Mature: 362

Protein sequence:

>362_residues
MKESLRLRLDQLCDRHEELTALLADAEVISDNKRFRKLSREHSDLNEIVDVWSKYRQAEEDIETAESMLSDPDFKDMAQE
EIKENRALIEQLEGDLNILMIPKDPNDANAAYLEVRAGTGGDEAAIFSGDLFRMYSKYAESRGWRIEVLSENEGEHGGYK
EVICRVDGDGVYGRLKFESGAHRVQRVPATESQGRVHTSACTVAILPEVDVDTTVEINPADLRIDTYRASGAGGQHINKT
DSAVRITHVPTGVVVECQEERSQHKNKAKAMALLVSRLENAKRAAQETATSEMRRDLVGSGDRSERIRTYNYPQGRMTDH
RINLTLYKLDAIMEGDLTELLDSLHREYQADQLAMLAQENGG

Sequences:

>Translated_362_residues
MKESLRLRLDQLCDRHEELTALLADAEVISDNKRFRKLSREHSDLNEIVDVWSKYRQAEEDIETAESMLSDPDFKDMAQE
EIKENRALIEQLEGDLNILMIPKDPNDANAAYLEVRAGTGGDEAAIFSGDLFRMYSKYAESRGWRIEVLSENEGEHGGYK
EVICRVDGDGVYGRLKFESGAHRVQRVPATESQGRVHTSACTVAILPEVDVDTTVEINPADLRIDTYRASGAGGQHINKT
DSAVRITHVPTGVVVECQEERSQHKNKAKAMALLVSRLENAKRAAQETATSEMRRDLVGSGDRSERIRTYNYPQGRMTDH
RINLTLYKLDAIMEGDLTELLDSLHREYQADQLAMLAQENGG
>Mature_362_residues
MKESLRLRLDQLCDRHEELTALLADAEVISDNKRFRKLSREHSDLNEIVDVWSKYRQAEEDIETAESMLSDPDFKDMAQE
EIKENRALIEQLEGDLNILMIPKDPNDANAAYLEVRAGTGGDEAAIFSGDLFRMYSKYAESRGWRIEVLSENEGEHGGYK
EVICRVDGDGVYGRLKFESGAHRVQRVPATESQGRVHTSACTVAILPEVDVDTTVEINPADLRIDTYRASGAGGQHINKT
DSAVRITHVPTGVVVECQEERSQHKNKAKAMALLVSRLENAKRAAQETATSEMRRDLVGSGDRSERIRTYNYPQGRMTDH
RINLTLYKLDAIMEGDLTELLDSLHREYQADQLAMLAQENGG

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=306, Percent_Identity=47.3856209150327, Blast_Score=271, Evalue=8e-73,
Organism=Homo sapiens, GI34577120, Length=331, Percent_Identity=40.1812688821752, Blast_Score=237, Evalue=1e-62,
Organism=Homo sapiens, GI166795305, Length=207, Percent_Identity=45.8937198067633, Blast_Score=184, Evalue=1e-46,
Organism=Escherichia coli, GI1787462, Length=359, Percent_Identity=62.116991643454, Blast_Score=474, Evalue=1e-135,
Organism=Escherichia coli, GI2367172, Length=340, Percent_Identity=39.1176470588235, Blast_Score=235, Evalue=4e-63,
Organism=Caenorhabditis elegans, GI17542784, Length=297, Percent_Identity=33.6700336700337, Blast_Score=170, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6321295, Length=317, Percent_Identity=39.7476340694006, Blast_Score=248, Evalue=1e-66,
Organism=Drosophila melanogaster, GI19921226, Length=321, Percent_Identity=38.9408099688474, Blast_Score=213, Evalue=2e-55,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_ACIAD (Q6F9S2)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_047013.2
- ProteinModelPortal:   Q6F9S2
- SMR:   Q6F9S2
- STRING:   Q6F9S2
- GeneID:   2880039
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD2418
- NMPDR:   fig|62977.3.peg.2300
- eggNOG:   COG0216
- HOGENOM:   HBG629764
- PhylomeDB:   Q6F9S2
- ProtClustDB:   PRK00591
- BioCyc:   ASP62977:ACIAD2418-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 40725; Mature: 40725

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKESLRLRLDQLCDRHEELTALLADAEVISDNKRFRKLSREHSDLNEIVDVWSKYRQAEE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DIETAESMLSDPDFKDMAQEEIKENRALIEQLEGDLNILMIPKDPNDANAAYLEVRAGTG
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEEEEECCCC
GDEAAIFSGDLFRMYSKYAESRGWRIEVLSENEGEHGGYKEVICRVDGDGVYGRLKFESG
CCCCEEECHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHEEEEECCCCEEEEEEECCC
AHRVQRVPATESQGRVHTSACTVAILPEVDVDTTVEINPADLRIDTYRASGAGGQHINKT
CHHHHCCCCCCCCCCEEECEEEEEEECCCCCCCEEEECCCCEEEEEEECCCCCCCCCCCC
DSAVRITHVPTGVVVECQEERSQHKNKAKAMALLVSRLENAKRAAQETATSEMRRDLVGS
CCEEEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GDRSERIRTYNYPQGRMTDHRINLTLYKLDAIMEGDLTELLDSLHREYQADQLAMLAQEN
CCCCCCCEECCCCCCCCCCEEEEEEEEEECHHHCCCHHHHHHHHHHHHHHHHHHHHHHCC
GG
CC
>Mature Secondary Structure
MKESLRLRLDQLCDRHEELTALLADAEVISDNKRFRKLSREHSDLNEIVDVWSKYRQAEE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DIETAESMLSDPDFKDMAQEEIKENRALIEQLEGDLNILMIPKDPNDANAAYLEVRAGTG
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEEEEECCCC
GDEAAIFSGDLFRMYSKYAESRGWRIEVLSENEGEHGGYKEVICRVDGDGVYGRLKFESG
CCCCEEECHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHEEEEECCCCEEEEEEECCC
AHRVQRVPATESQGRVHTSACTVAILPEVDVDTTVEINPADLRIDTYRASGAGGQHINKT
CHHHHCCCCCCCCCCEEECEEEEEEECCCCCCCEEEECCCCEEEEEEECCCCCCCCCCCC
DSAVRITHVPTGVVVECQEERSQHKNKAKAMALLVSRLENAKRAAQETATSEMRRDLVGS
CCEEEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GDRSERIRTYNYPQGRMTDHRINLTLYKLDAIMEGDLTELLDSLHREYQADQLAMLAQEN
CCCCCCCEECCCCCCCCCCEEEEEEEEEECHHHCCCHHHHHHHHHHHHHHHHHHHHHHCC
GG
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA