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The map label for this gene is gidB

Identifier: 50085458

GI number: 50085458

Start: 2326039

End: 2326677

Strand: Reverse

Name: gidB

Synonym: ACIAD2368

Alternate gene names: 50085458

Gene position: 2326677-2326039 (Counterclockwise)

Preceding gene: 50085459

Following gene: 50085457

Centisome position: 64.65

GC content: 40.38

Gene sequence:

>639_bases
ATGCATCCATTTTTGCAAGAGTTAAAACAAGGCAGTCAGGCATTAGGTCTGGATCTGAGTGATGAAGTACTTGGATTGTT
ATTAAAATATCAAGATGCTTTGGTGTTGTGGAACAAAGCATATAATTTGACTGCGATACGCGATCCTAAAGAAATGCTGG
TTAAACATCTATTGGATAGCTTAAGTATTTTAAAAGATCTGCCGCAAGGTCGCCTGTTGGATGTGGGAACAGGTGGCGGT
ATGCCAGGTATGATTATTGCGCTATGTCAGCCTGAACGCAATTGTGTTTTGCTCGACTCAAATGGTAAGAAAATTCGCTT
TCTCAAGCAATTTATTGCAGATTTGAAGCTTAAGAATGTGGTTGCTGTGCAAACACGTGTTGAAAATGAAGAGTCTATTC
AAGAGCTTGGGAAGTTTGATGTCATCACCAGTCGTGCGTTTGCCTCGTTGCTCGATTTTGTCGATGCAGCACGCCCTTAT
ATGTATGAATCAACCATTATTGCCGCGATGAAAGGTCTTGTGCCGACTGAAGAAATGCAACAACTTAAAGCAGAATTTTC
TTGTCAGGTCATTGGATTGCAAGTTCCGCGTTTGGATGAGCAGCGTCATTTGCTTTTACTTCAACAAATAAAAAATTAA

Upstream 100 bases:

>100_bases
GGGTTTATATGCTTAACTGGGTATTCACTAATCGGCAGAGACTAGGCGTAGACCGAGAAATCGGTATACTTCCTCTAAAT
TTTTAAGATGTGAAGCGGAT

Downstream 100 bases:

>100_bases
GTTTGGGGTTATCATGGCTCAGATTATTGCAATTGCAAACCAGAAAGGTGGCGTAGGTAAAACGACGACAGCTGTGAATC
TGGCTGCCTCTTTAGCTGTA

Product: 16S rRNA methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MHPFLQELKQGSQALGLDLSDEVLGLLLKYQDALVLWNKAYNLTAIRDPKEMLVKHLLDSLSILKDLPQGRLLDVGTGGG
MPGMIIALCQPERNCVLLDSNGKKIRFLKQFIADLKLKNVVAVQTRVENEESIQELGKFDVITSRAFASLLDFVDAARPY
MYESTIIAAMKGLVPTEEMQQLKAEFSCQVIGLQVPRLDEQRHLLLLQQIKN

Sequences:

>Translated_212_residues
MHPFLQELKQGSQALGLDLSDEVLGLLLKYQDALVLWNKAYNLTAIRDPKEMLVKHLLDSLSILKDLPQGRLLDVGTGGG
MPGMIIALCQPERNCVLLDSNGKKIRFLKQFIADLKLKNVVAVQTRVENEESIQELGKFDVITSRAFASLLDFVDAARPY
MYESTIIAAMKGLVPTEEMQQLKAEFSCQVIGLQVPRLDEQRHLLLLQQIKN
>Mature_212_residues
MHPFLQELKQGSQALGLDLSDEVLGLLLKYQDALVLWNKAYNLTAIRDPKEMLVKHLLDSLSILKDLPQGRLLDVGTGGG
MPGMIIALCQPERNCVLLDSNGKKIRFLKQFIADLKLKNVVAVQTRVENEESIQELGKFDVITSRAFASLLDFVDAARPY
MYESTIIAAMKGLVPTEEMQQLKAEFSCQVIGLQVPRLDEQRHLLLLQQIKN

Specific function: Specifically methylates the N7 position of guanosine in position 527 of 16S rRNA

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family

Homologues:

Organism=Escherichia coli, GI1790179, Length=197, Percent_Identity=44.1624365482233, Blast_Score=159, Evalue=9e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMG_ACIAD (Q6F9W7)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_046968.1
- ProteinModelPortal:   Q6F9W7
- SMR:   Q6F9W7
- STRING:   Q6F9W7
- GeneID:   2881135
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD2368
- NMPDR:   fig|62977.3.peg.2255
- eggNOG:   COG0357
- HOGENOM:   HBG686577
- OMA:   YELLVEW
- PhylomeDB:   Q6F9W7
- ProtClustDB:   PRK00107
- BioCyc:   ASP62977:ACIAD2368-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00074
- InterPro:   IPR003682
- PIRSF:   PIRSF003078
- TIGRFAMs:   TIGR00138

Pfam domain/function: PF02527 GidB

EC number: =2.1.1.170

Molecular weight: Translated: 23802; Mature: 23802

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHPFLQELKQGSQALGLDLSDEVLGLLLKYQDALVLWNKAYNLTAIRDPKEMLVKHLLDS
CCHHHHHHHCCCHHCCCCCHHHHHHHHHHHHHHHEEEECCCCEEEECCHHHHHHHHHHHH
LSILKDLPQGRLLDVGTGGGMPGMIIALCQPERNCVLLDSNGKKIRFLKQFIADLKLKNV
HHHHHHCCCCCEEEECCCCCCCCEEEEEECCCCCEEEECCCCCCHHHHHHHHHHHHHHHH
VAVQTRVENEESIQELGKFDVITSRAFASLLDFVDAARPYMYESTIIAAMKGLVPTEEMQ
HEEHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHH
QLKAEFSCQVIGLQVPRLDEQRHLLLLQQIKN
HHHHHHCEEEEEEECCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MHPFLQELKQGSQALGLDLSDEVLGLLLKYQDALVLWNKAYNLTAIRDPKEMLVKHLLDS
CCHHHHHHHCCCHHCCCCCHHHHHHHHHHHHHHHEEEECCCCEEEECCHHHHHHHHHHHH
LSILKDLPQGRLLDVGTGGGMPGMIIALCQPERNCVLLDSNGKKIRFLKQFIADLKLKNV
HHHHHHCCCCCEEEECCCCCCCCEEEEEECCCCCEEEECCCCCCHHHHHHHHHHHHHHHH
VAVQTRVENEESIQELGKFDVITSRAFASLLDFVDAARPYMYESTIIAAMKGLVPTEEMQ
HEEHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHH
QLKAEFSCQVIGLQVPRLDEQRHLLLLQQIKN
HHHHHHCEEEEEEECCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA