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The map label for this gene is holB [H]

Identifier: 50085452

GI number: 50085452

Start: 2319517

End: 2320512

Strand: Reverse

Name: holB [H]

Synonym: ACIAD2361

Alternate gene names: 50085452

Gene position: 2320512-2319517 (Counterclockwise)

Preceding gene: 50085453

Following gene: 50085451

Centisome position: 64.48

GC content: 40.06

Gene sequence:

>996_bases
TTGGTGAGTTTGAATAAACAAGAAAATCTGGTGTATCCATGGCAGGAAAATGTCTGGACAACGCTTACCCGCCGTTTCCC
GCAAGTGGGGCATGGGCTTTTATTTTATGGAAAGAAGGGTTGCGGTAAAGATGCTTTCACACAGCATTTTCTGGCGTGGG
TGCTGTGTACCCATAAAAAACCAGAGGGTGCATGTGGTGAGTGTGTAAGTTGTCAGTGGCTTAAGTCGGATACACATCCC
AATTATGTGCATATCACTACAGATGAAGACAATAAAAAGCAAAATGCCAAAATCAAGATAGAAAAAATACGTGAACTTCT
ACCTTTTGTGCAGCAAACGGTAGATGGTTGGCGTGTCATTGTGATTGAACCCGCAGAAGCACTAAACATCGCTTCATCTA
ATGCACTGTTGAAAACTCTTGAAGAGCCAGGCGAGCGTATTTTGATTGTGTTGCTGGCAGACCACTATTTAAAACTTCCT
GCCACGATTCGTAGTCGTTTACAGCACTTCTCTCTGGATCGGGTTTCTACTGTTCAGGCACATGACTATCTACAGCAGCA
CCTTGACAATCTACCTTCTGAGCAAGCACAGCTTTTACTTAATCTGACCAATAATATGCCGCTTGAGGCGATTGAGGTTG
CCAAATCTGATTGGTTTGCTAAACGTGAAGCATTTTTGATGGACTGGATCAAGCTGGTACATCAAAAAAATATGCCGTTA
TTCTATTCATCAAAGTGGCAAAAAGAGCTGGTTTTTTCAGAATGGTTGACATTATTTGAATACTTGTTGTGTGATTTAAT
TTGTGTCAAGCTAGATCAGGTCTGTAAGAATACTGATCTGGATTTAAAGGATTTAGCATCAAATTATACATTGGAAGTGC
TTTTTGATTTCTATGCCGATTTACAACATGCCAAACAGTTGATTGAACAAAATGTGCAGTCAAATCTTGTAATGGATCAG
TTGTTTATTAAACTTATGAATGTGGATGGTGGATAA

Upstream 100 bases:

>100_bases
AATTGCATTGGCTGAGGTCAATTTACCGCCAGGTGTCGATACGCCAGCCGATTTAGAGCGTTTGAATGCTTTGCCTCGTT
CTTATTTTGAATAAGATGGA

Downstream 100 bases:

>100_bases
ACATGGATTCAAGAATCAATGGTGGTCTGATTCATGTCAATATTCCTGATCGCGTGACGCTGCAAGCCAGCTATATGAGT
TTTATACAAGGTGGTGGCTT

Product: DNA polymerase III delta'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MVSLNKQENLVYPWQENVWTTLTRRFPQVGHGLLFYGKKGCGKDAFTQHFLAWVLCTHKKPEGACGECVSCQWLKSDTHP
NYVHITTDEDNKKQNAKIKIEKIRELLPFVQQTVDGWRVIVIEPAEALNIASSNALLKTLEEPGERILIVLLADHYLKLP
ATIRSRLQHFSLDRVSTVQAHDYLQQHLDNLPSEQAQLLLNLTNNMPLEAIEVAKSDWFAKREAFLMDWIKLVHQKNMPL
FYSSKWQKELVFSEWLTLFEYLLCDLICVKLDQVCKNTDLDLKDLASNYTLEVLFDFYADLQHAKQLIEQNVQSNLVMDQ
LFIKLMNVDGG

Sequences:

>Translated_331_residues
MVSLNKQENLVYPWQENVWTTLTRRFPQVGHGLLFYGKKGCGKDAFTQHFLAWVLCTHKKPEGACGECVSCQWLKSDTHP
NYVHITTDEDNKKQNAKIKIEKIRELLPFVQQTVDGWRVIVIEPAEALNIASSNALLKTLEEPGERILIVLLADHYLKLP
ATIRSRLQHFSLDRVSTVQAHDYLQQHLDNLPSEQAQLLLNLTNNMPLEAIEVAKSDWFAKREAFLMDWIKLVHQKNMPL
FYSSKWQKELVFSEWLTLFEYLLCDLICVKLDQVCKNTDLDLKDLASNYTLEVLFDFYADLQHAKQLIEQNVQSNLVMDQ
LFIKLMNVDGG
>Mature_331_residues
MVSLNKQENLVYPWQENVWTTLTRRFPQVGHGLLFYGKKGCGKDAFTQHFLAWVLCTHKKPEGACGECVSCQWLKSDTHP
NYVHITTDEDNKKQNAKIKIEKIRELLPFVQQTVDGWRVIVIEPAEALNIASSNALLKTLEEPGERILIVLLADHYLKLP
ATIRSRLQHFSLDRVSTVQAHDYLQQHLDNLPSEQAQLLLNLTNNMPLEAIEVAKSDWFAKREAFLMDWIKLVHQKNMPL
FYSSKWQKELVFSEWLTLFEYLLCDLICVKLDQVCKNTDLDLKDLASNYTLEVLFDFYADLQHAKQLIEQNVQSNLVMDQ
LFIKLMNVDGG

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786676, Length=179, Percent_Identity=30.1675977653631, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003959
- InterPro:   IPR004622 [H]

Pfam domain/function: PF00004 AAA [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 38333; Mature: 38333

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSLNKQENLVYPWQENVWTTLTRRFPQVGHGLLFYGKKGCGKDAFTQHFLAWVLCTHKK
CCCCCCCCCEECCCHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHCCC
PEGACGECVSCQWLKSDTHPNYVHITTDEDNKKQNAKIKIEKIRELLPFVQQTVDGWRVI
CCCCCCCCCCCEEECCCCCCCEEEEECCCCCCCCCCCEEHHHHHHHHHHHHHHCCCCEEE
VIEPAEALNIASSNALLKTLEEPGERILIVLLADHYLKLPATIRSRLQHFSLDRVSTVQA
EECCHHHHHCCCHHHHHHHHHCCCCEEEEEEECCHHHCCCHHHHHHHHHCCCHHHHHHHH
HDYLQQHLDNLPSEQAQLLLNLTNNMPLEAIEVAKSDWFAKREAFLMDWIKLVHQKNMPL
HHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
FYSSKWQKELVFSEWLTLFEYLLCDLICVKLDQVCKNTDLDLKDLASNYTLEVLFDFYAD
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCHHHHHHHHHHH
LQHAKQLIEQNVQSNLVMDQLFIKLMNVDGG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MVSLNKQENLVYPWQENVWTTLTRRFPQVGHGLLFYGKKGCGKDAFTQHFLAWVLCTHKK
CCCCCCCCCEECCCHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHCCC
PEGACGECVSCQWLKSDTHPNYVHITTDEDNKKQNAKIKIEKIRELLPFVQQTVDGWRVI
CCCCCCCCCCCEEECCCCCCCEEEEECCCCCCCCCCCEEHHHHHHHHHHHHHHCCCCEEE
VIEPAEALNIASSNALLKTLEEPGERILIVLLADHYLKLPATIRSRLQHFSLDRVSTVQA
EECCHHHHHCCCHHHHHHHHHCCCCEEEEEEECCHHHCCCHHHHHHHHHCCCHHHHHHHH
HDYLQQHLDNLPSEQAQLLLNLTNNMPLEAIEVAKSDWFAKREAFLMDWIKLVHQKNMPL
HHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
FYSSKWQKELVFSEWLTLFEYLLCDLICVKLDQVCKNTDLDLKDLASNYTLEVLFDFYAD
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCHHHHHHHHHHH
LQHAKQLIEQNVQSNLVMDQLFIKLMNVDGG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8550441; 10984043 [H]