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The map label for this gene is kdsB

Identifier: 50085453

GI number: 50085453

Start: 2320519

End: 2321280

Strand: Reverse

Name: kdsB

Synonym: ACIAD2362

Alternate gene names: 50085453

Gene position: 2321280-2320519 (Counterclockwise)

Preceding gene: 50085454

Following gene: 50085452

Centisome position: 64.5

GC content: 45.41

Gene sequence:

>762_bases
ATGAAACATATCGTCATCCCGGCGCGTTTTTCAAGCACTCGCTTGCCAGGCAAACCTTTACTTGAAATTCATGGGCGCGC
CATGATTTTACGTGTTGTCGATCAGGCAAAAAAAGTGCAGGGTTTTGATGACTTGTGTGTGGCAACAGACAATGAGCGAA
TCATGCAGCTGTGTCAGGCAGAAGGTGTTGATGTGGTGCTGACGAGTCCAGACCATCCCTCAGGCACAGATCGTTTGAGT
GAAGTGGCTCGATTAAAGGGCTGGTCTGAAGATGATATTATTGTCAATGTGCAAGGTGACGAGCCTTTATTGCCAGCAAC
GTTGGTTCAGCAGGTAGCAGAGTTGCTGGTTGCAAAACCAAATTGTTCGATGTCGACATTATGTGAACCGATCCATACGC
TAGACGAATTTCAGCGTGATAGTATTGTAAAAGTCGTCATGTCCCAATATCAGGAAGCATTATATTTTAGCCGTGCAACG
ATTCCATATGATCGAGATGCTGCTCAAACACAACAGTCATCACGTTTCGAGCATGCTTATCGGCATTTAGGTCTATATGC
ATATCGAGTTGGCCTGTTGCAAGAGTATGTGGCTTGGCAGCAAGGTGTTTTAGAAAAACTGGAAAGTCTTGAGCAGCTTC
GGGTTCTGGAAAATGGTCATCGGATTGCAATTGCATTGGCTGAGGTCAATTTACCGCCAGGTGTCGATACGCCAGCCGAT
TTAGAGCGTTTGAATGCTTTGCCTCGTTCTTATTTTGAATAA

Upstream 100 bases:

>100_bases
TTGGGTGGTGCCTGTCGAGGCTGTCATGTCTGACGCATGTTACATCCGACTTGAACAACAGTTAGCACAGTTGGGTGTTT
GCTTTTCTTAGGAACTAATC

Downstream 100 bases:

>100_bases
GATGGATTGGTGAGTTTGAATAAACAAGAAAATCTGGTGTATCCATGGCAGGAAAATGTCTGGACAACGCTTACCCGCCG
TTTCCCGCAAGTGGGGCATG

Product: 3-deoxy-manno-octulosonate cytidylyltransferase

Products: NA

Alternate protein names: CMP-2-keto-3-deoxyoctulosonic acid synthase; CKS; CMP-KDO synthase

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MKHIVIPARFSSTRLPGKPLLEIHGRAMILRVVDQAKKVQGFDDLCVATDNERIMQLCQAEGVDVVLTSPDHPSGTDRLS
EVARLKGWSEDDIIVNVQGDEPLLPATLVQQVAELLVAKPNCSMSTLCEPIHTLDEFQRDSIVKVVMSQYQEALYFSRAT
IPYDRDAAQTQQSSRFEHAYRHLGLYAYRVGLLQEYVAWQQGVLEKLESLEQLRVLENGHRIAIALAEVNLPPGVDTPAD
LERLNALPRSYFE

Sequences:

>Translated_253_residues
MKHIVIPARFSSTRLPGKPLLEIHGRAMILRVVDQAKKVQGFDDLCVATDNERIMQLCQAEGVDVVLTSPDHPSGTDRLS
EVARLKGWSEDDIIVNVQGDEPLLPATLVQQVAELLVAKPNCSMSTLCEPIHTLDEFQRDSIVKVVMSQYQEALYFSRAT
IPYDRDAAQTQQSSRFEHAYRHLGLYAYRVGLLQEYVAWQQGVLEKLESLEQLRVLENGHRIAIALAEVNLPPGVDTPAD
LERLNALPRSYFE
>Mature_253_residues
MKHIVIPARFSSTRLPGKPLLEIHGRAMILRVVDQAKKVQGFDDLCVATDNERIMQLCQAEGVDVVLTSPDHPSGTDRLS
EVARLKGWSEDDIIVNVQGDEPLLPATLVQQVAELLVAKPNCSMSTLCEPIHTLDEFQRDSIVKVVMSQYQEALYFSRAT
IPYDRDAAQTQQSSRFEHAYRHLGLYAYRVGLLQEYVAWQQGVLEKLESLEQLRVLENGHRIAIALAEVNLPPGVDTPAD
LERLNALPRSYFE

Specific function: Activates KDO (a required 8-carbon sugar) for incorporation into bacterial lipopolysaccharide in Gram-negative bacteria

COG id: COG1212

COG function: function code M; CMP-2-keto-3-deoxyoctulosonic acid synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the kdsB family

Homologues:

Organism=Escherichia coli, GI1787147, Length=243, Percent_Identity=48.1481481481481, Blast_Score=226, Evalue=1e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KDSB_ACIAD (Q6F9X2)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_046963.1
- HSSP:   P04951
- ProteinModelPortal:   Q6F9X2
- SMR:   Q6F9X2
- STRING:   Q6F9X2
- GeneID:   2879979
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD2362
- NMPDR:   fig|62977.3.peg.2250
- eggNOG:   COG1212
- HOGENOM:   HBG637773
- OMA:   FSRAPLP
- PhylomeDB:   Q6F9X2
- ProtClustDB:   PRK05450
- BioCyc:   ASP62977:ACIAD2362-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00057
- InterPro:   IPR003329
- InterPro:   IPR004528
- TIGRFAMs:   TIGR00466

Pfam domain/function: PF02348 CTP_transf_3

EC number: =2.7.7.38

Molecular weight: Translated: 28446; Mature: 28446

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHIVIPARFSSTRLPGKPLLEIHGRAMILRVVDQAKKVQGFDDLCVATDNERIMQLCQA
CCEEEEECCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHH
EGVDVVLTSPDHPSGTDRLSEVARLKGWSEDDIIVNVQGDEPLLPATLVQQVAELLVAKP
CCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCC
NCSMSTLCEPIHTLDEFQRDSIVKVVMSQYQEALYFSRATIPYDRDAAQTQQSSRFEHAY
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
RHLGLYAYRVGLLQEYVAWQQGVLEKLESLEQLRVLENGHRIAIALAEVNLPPGVDTPAD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCHHH
LERLNALPRSYFE
HHHHHHHHHHHCC
>Mature Secondary Structure
MKHIVIPARFSSTRLPGKPLLEIHGRAMILRVVDQAKKVQGFDDLCVATDNERIMQLCQA
CCEEEEECCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHH
EGVDVVLTSPDHPSGTDRLSEVARLKGWSEDDIIVNVQGDEPLLPATLVQQVAELLVAKP
CCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCC
NCSMSTLCEPIHTLDEFQRDSIVKVVMSQYQEALYFSRATIPYDRDAAQTQQSSRFEHAY
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
RHLGLYAYRVGLLQEYVAWQQGVLEKLESLEQLRVLENGHRIAIALAEVNLPPGVDTPAD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCHHH
LERLNALPRSYFE
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA