Click here to switch to the map view.

The map label for this gene is pyrF

Identifier: 50085432

GI number: 50085432

Start: 2305660

End: 2306343

Strand: Reverse

Name: pyrF

Synonym: ACIAD2341

Alternate gene names: 50085432

Gene position: 2306343-2305660 (Counterclockwise)

Preceding gene: 50085433

Following gene: 50085429

Centisome position: 64.09

GC content: 42.54

Gene sequence:

>684_bases
GTGAGTATTATTGTTGCCTTAGACGCAAAGAGCCAATATGACGCTTTGAGCATTGCTGATCAGCTCGATCCATCACTTTG
TCGTGTAAAAGTGGGCAAAGAGCTTTTTACTCATGATGGTCCACAAACAGTAAGAATCTTACAAGATCGTGGTTTTGAGT
TGTTTTTAGACCTTAAATTTCATGACATTCCAAACACCACAGCACAGGCTGTATGTGCTGCGGCAGACTTAGGTGTATGG
ATGGTCAATGTTCATGCGTCTGGCGGTCGTAAAATGATGGAAACCTGTGTTGAACGACTTAAAGCTGGAAATTACCGTAC
TCAACTTATTGCGGTAACGGTATTAACCTCTATGGGGCGTGAAGATTTACGTGACATTGGTTTAGATGTTGAACCTTTTG
AGCAAGTTAAACGTCTGGCACAGCTAACGCAAGAAAGCGGTCTGGATGGTGTGGTATGTTCTGCTCAAGAAGCAAAAATG
TTACGTGATTTACTAGGAACAGAATTTGCTTTGGTCACTCCAGGTATTCGACCTGAGGGTGCAAATGCAGATGACCAAAA
ACGCATTGTGACGCCAAAGCAGGCAATGTTGGATGGTTCGACTCATCTAGTCATTGGTCGTCCAATTACAAAATCCGAAC
GACCAAATGAAATGTTGAAACAGATTTTATCGAGTCTGGCTTAA

Upstream 100 bases:

>100_bases
TCATAATTTGGGCTTTTTTTCATAAAAGCGATATTCCTGCTTTGTCGAATACGTGAATCCATCTATAATTAGCCGAATTT
TAAGCTGTGGAAATAGATTC

Downstream 100 bases:

>100_bases
GCTATTAAAATTAGGCTGATCATGATTGGAGCCAGTAAGCTTAAGATCACGCCGCTGACCATAGCATGTGGTACATAATG
CGTACCACATGATTGTTTCA

Product: orotidine 5'-phosphate decarboxylase

Products: NA

Alternate protein names: OMP decarboxylase; OMPDCase; OMPdecase

Number of amino acids: Translated: 227; Mature: 226

Protein sequence:

>227_residues
MSIIVALDAKSQYDALSIADQLDPSLCRVKVGKELFTHDGPQTVRILQDRGFELFLDLKFHDIPNTTAQAVCAAADLGVW
MVNVHASGGRKMMETCVERLKAGNYRTQLIAVTVLTSMGREDLRDIGLDVEPFEQVKRLAQLTQESGLDGVVCSAQEAKM
LRDLLGTEFALVTPGIRPEGANADDQKRIVTPKQAMLDGSTHLVIGRPITKSERPNEMLKQILSSLA

Sequences:

>Translated_227_residues
MSIIVALDAKSQYDALSIADQLDPSLCRVKVGKELFTHDGPQTVRILQDRGFELFLDLKFHDIPNTTAQAVCAAADLGVW
MVNVHASGGRKMMETCVERLKAGNYRTQLIAVTVLTSMGREDLRDIGLDVEPFEQVKRLAQLTQESGLDGVVCSAQEAKM
LRDLLGTEFALVTPGIRPEGANADDQKRIVTPKQAMLDGSTHLVIGRPITKSERPNEMLKQILSSLA
>Mature_226_residues
SIIVALDAKSQYDALSIADQLDPSLCRVKVGKELFTHDGPQTVRILQDRGFELFLDLKFHDIPNTTAQAVCAAADLGVWM
VNVHASGGRKMMETCVERLKAGNYRTQLIAVTVLTSMGREDLRDIGLDVEPFEQVKRLAQLTQESGLDGVVCSAQEAKML
RDLLGTEFALVTPGIRPEGANADDQKRIVTPKQAMLDGSTHLVIGRPITKSERPNEMLKQILSSLA

Specific function: Catalyzes the decarboxylation of orotidine 5'- monophosphate (OMP) to uridine 5'-monophosphate (UMP)

COG id: COG0284

COG function: function code F; Orotidine-5'-phosphate decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the OMP decarboxylase family. Type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1787537, Length=225, Percent_Identity=59.5555555555556, Blast_Score=253, Evalue=6e-69,

Paralogues:

None

Copy number: 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): PYRF_ACIAD (Q6F9Z3)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_046942.1
- ProteinModelPortal:   Q6F9Z3
- SMR:   Q6F9Z3
- STRING:   Q6F9Z3
- GeneID:   2879567
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD2341
- NMPDR:   fig|62977.3.peg.2229
- eggNOG:   COG0284
- HOGENOM:   HBG625253
- OMA:   TVHAYPQ
- PhylomeDB:   Q6F9Z3
- ProtClustDB:   PRK00230
- BioCyc:   ASP62977:ACIAD2341-MONOMER
- HAMAP:   MF_01200_B
- InterPro:   IPR013785
- InterPro:   IPR014732
- InterPro:   IPR018089
- InterPro:   IPR001754
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00934
- TIGRFAMs:   TIGR01740

Pfam domain/function: PF00215 OMPdecase; SSF51366 RibP_bind_barrel

EC number: =4.1.1.23

Molecular weight: Translated: 24887; Mature: 24756

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: PS00156 OMPDECASE

Important sites: ACT_SITE 59-59 BINDING 8-8 BINDING 30-30 BINDING 116-116 BINDING 177-177 BINDING 186-186 BINDING 206-206 BINDING 207-207

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIIVALDAKSQYDALSIADQLDPSLCRVKVGKELFTHDGPQTVRILQDRGFELFLDLKF
CEEEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEEEEEEE
HDIPNTTAQAVCAAADLGVWMVNVHASGGRKMMETCVERLKAGNYRTQLIAVTVLTSMGR
CCCCCHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCH
EDLRDIGLDVEPFEQVKRLAQLTQESGLDGVVCSAQEAKMLRDLLGTEFALVTPGIRPEG
HHHHHCCCCCCHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHCCCEEEECCCCCCCC
ANADDQKRIVTPKQAMLDGSTHLVIGRPITKSERPNEMLKQILSSLA
CCCCCCCEECCCHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
SIIVALDAKSQYDALSIADQLDPSLCRVKVGKELFTHDGPQTVRILQDRGFELFLDLKF
EEEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEEEEEEE
HDIPNTTAQAVCAAADLGVWMVNVHASGGRKMMETCVERLKAGNYRTQLIAVTVLTSMGR
CCCCCHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCH
EDLRDIGLDVEPFEQVKRLAQLTQESGLDGVVCSAQEAKMLRDLLGTEFALVTPGIRPEG
HHHHHCCCCCCHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHCCCEEEECCCCCCCC
ANADDQKRIVTPKQAMLDGSTHLVIGRPITKSERPNEMLKQILSSLA
CCCCCCCEECCCHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA