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The map label for this gene is rsmE [H]

Identifier: 50085384

GI number: 50085384

Start: 2255364

End: 2256095

Strand: Direct

Name: rsmE [H]

Synonym: ACIAD2284

Alternate gene names: 50085384

Gene position: 2255364-2256095 (Clockwise)

Preceding gene: 50085383

Following gene: 50085385

Centisome position: 62.67

GC content: 36.89

Gene sequence:

>732_bases
ATGAATCGTTTTTATATTGAAGCCGATCTAAGTGTAAACACAGATACTCTCTTAACTGAATCTGTGTTTCATCATTGGGT
AAGAGTACTTCGTGCTCAGGTAAATGACTGTGCTATTTTCTTCAATGGTCAAGGTGGTGAATATCAAGTCACCCTGCAAG
AAATTAATAAAAAAAATGCCACGGTCTCTATCGATCAATTTAATCCTGTAGATCGTACACCAGCGTTTAAAGCCATACTT
GGACAAGTCATGAGCAAGGGTGATCGCATGGATTATGCAATTCAAAAAGCAACTGAATTAGGCGTATCGAAAATTCAGTT
GTTGACCAGTGAACGCTGTGAAATGCGTTTAAAATATGATCGTGACCAAAAAAAAATTGATCATTGGCAAAGTATTGCAA
TTGCAGCATGTGAACAATGTGGAATGAATCGAGTACCTGAAATTTTACCTCCTGTTTCTTTAAATGAATGGATTAACACA
GCTACACTTCCTAATACAAAACTTGTGTTAGCCCCAGAAAAAGAACAACAAAATGTACTCAAAAATGCACAAAGTGAACT
TGCGCTTCTTATTGGACCTGAAGGCGGATTGAGCGAAAATGAAATCATCGCAGCAAACAACAAAGGCTTTATAAATTGGT
GTATTGGTGAACGAGTCTTACGTACAGAAACAGCTCCTGTCGTTGCACTTTCTATTTTAAATTACCGTTTTTTATCAGAA
CTTGAATTGTGA

Upstream 100 bases:

>100_bases
GCTCCTAGCCTGCATTTCTATTCAATGAATAACACAGAACCAACTCGTCAACTTGTGATTGACTTAGGTTTGAATTAATT
TTTAAAAGAGCAATGCCCCA

Downstream 100 bases:

>100_bases
ATATTGATTTTAAAATGTGACTGAGTGAATTAGAATTTGATAATTGATATATCAGTAATGATCAGGAAACCCTACTGATG
TTGAACTTATCATCTCATTT

Product: hypothetical protein

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MNRFYIEADLSVNTDTLLTESVFHHWVRVLRAQVNDCAIFFNGQGGEYQVTLQEINKKNATVSIDQFNPVDRTPAFKAIL
GQVMSKGDRMDYAIQKATELGVSKIQLLTSERCEMRLKYDRDQKKIDHWQSIAIAACEQCGMNRVPEILPPVSLNEWINT
ATLPNTKLVLAPEKEQQNVLKNAQSELALLIGPEGGLSENEIIAANNKGFINWCIGERVLRTETAPVVALSILNYRFLSE
LEL

Sequences:

>Translated_243_residues
MNRFYIEADLSVNTDTLLTESVFHHWVRVLRAQVNDCAIFFNGQGGEYQVTLQEINKKNATVSIDQFNPVDRTPAFKAIL
GQVMSKGDRMDYAIQKATELGVSKIQLLTSERCEMRLKYDRDQKKIDHWQSIAIAACEQCGMNRVPEILPPVSLNEWINT
ATLPNTKLVLAPEKEQQNVLKNAQSELALLIGPEGGLSENEIIAANNKGFINWCIGERVLRTETAPVVALSILNYRFLSE
LEL
>Mature_243_residues
MNRFYIEADLSVNTDTLLTESVFHHWVRVLRAQVNDCAIFFNGQGGEYQVTLQEINKKNATVSIDQFNPVDRTPAFKAIL
GQVMSKGDRMDYAIQKATELGVSKIQLLTSERCEMRLKYDRDQKKIDHWQSIAIAACEQCGMNRVPEILPPVSLNEWINT
ATLPNTKLVLAPEKEQQNVLKNAQSELALLIGPEGGLSENEIIAANNKGFINWCIGERVLRTETAPVVALSILNYRFLSE
LEL

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=236, Percent_Identity=40.2542372881356, Blast_Score=161, Evalue=4e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 27394; Mature: 27394

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRFYIEADLSVNTDTLLTESVFHHWVRVLRAQVNDCAIFFNGQGGEYQVTLQEINKKNA
CCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEEEHHHCCCCCC
TVSIDQFNPVDRTPAFKAILGQVMSKGDRMDYAIQKATELGVSKIQLLTSERCEMRLKYD
EEEEECCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHHHHHHHHHEEECCC
RDQKKIDHWQSIAIAACEQCGMNRVPEILPPVSLNEWINTATLPNTKLVLAPEKEQQNVL
CHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCCHHHHHCCCCCCCCEEEECCCHHHHHHH
KNAQSELALLIGPEGGLSENEIIAANNKGFINWCIGERVLRTETAPVVALSILNYRFLSE
HCCCCCEEEEECCCCCCCCCCEEEECCCCEEEEECCCHHHHCCCCHHHHHHHHHHHHHHH
LEL
CCC
>Mature Secondary Structure
MNRFYIEADLSVNTDTLLTESVFHHWVRVLRAQVNDCAIFFNGQGGEYQVTLQEINKKNA
CCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEEEHHHCCCCCC
TVSIDQFNPVDRTPAFKAILGQVMSKGDRMDYAIQKATELGVSKIQLLTSERCEMRLKYD
EEEEECCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHHHHHHHHHEEECCC
RDQKKIDHWQSIAIAACEQCGMNRVPEILPPVSLNEWINTATLPNTKLVLAPEKEQQNVL
CHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCCHHHHHCCCCCCCCEEEECCCHHHHHHH
KNAQSELALLIGPEGGLSENEIIAANNKGFINWCIGERVLRTETAPVVALSILNYRFLSE
HCCCCCEEEEECCCCCCCCCCEEEECCCCEEEEECCCHHHHCCCCHHHHHHHHHHHHHHH
LEL
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8332061 [H]